3032-0951

[[amino-(4-amino-1,2,5-oxadiazol-3-yl)methylidene]amino] 2-chlorobenzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_727
Screen concentration 202.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3652836
SMILES C1=CC=C(C(=C1)C(=O)ON=C(C2=NON=C2N)N)Cl
Standardized SMILES NC(=NOC(=O)c1ccccc1Cl)c2nonc2N
Molecular weight 281.6552
ALogP 1.08
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.16
% growth inhibition (Hom. pool) 13.08


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3652836
Download HIP data (tab-delimited text)  (excel)
Gene:ERG12(YMR208W)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.03||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRS1(YLR060W)|FD-Score:4.58|P-value:2.28E-6|Clearance:1.28||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:IMP3(YHR148W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:PTI1(YGR156W)|FD-Score:5.1|P-value:1.66E-7|Clearance:1.28||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:SEC13(YLR208W)|FD-Score:4.76|P-value:9.67E-7|Clearance:1.28||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TIF11(YMR260C)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.2||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:ERG12(YMR208W)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.03||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRS1(YLR060W)|FD-Score:4.58|P-value:2.28E-6|Clearance:1.28||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:IMP3(YHR148W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:PTI1(YGR156W)|FD-Score:5.1|P-value:1.66E-7|Clearance:1.28||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:SEC13(YLR208W)|FD-Score:4.76|P-value:9.67E-7|Clearance:1.28||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TIF11(YMR260C)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.2||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3652836
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM18(YHR198C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALE1(YOR175C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ANT1(YPR128C)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APT2(YDR441C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ARL1(YBR164C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO3(YDR035W)|FD-Score:6.26|P-value:1.87E-10||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG13(YPR185W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:BAT2(YJR148W)|FD-Score:-4.03|P-value:2.75E-5||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BTS1(YPL069C)|FD-Score:8.83|P-value:5.24E-19||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DIG2(YDR480W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOT6(YER088C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:FLC2(YAL053W)|FD-Score:4.27|P-value:9.67E-6||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP16(YDR070C_p)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FUN26(YAL022C)|FD-Score:-4.48|P-value:3.69E-6||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GCN2(YDR283C)|FD-Score:5.29|P-value:6.09E-8||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.15|P-value:1.27E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP3(YOR205C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GND2(YGR256W)|FD-Score:4.46|P-value:4.14E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GPG1(YGL121C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HCR1(YLR192C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IRA2(YOL081W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MGA2(YIR033W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MIC23(YBL107C_p)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MRC1(YCL061C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL22(YNL177C)|FD-Score:5.6|P-value:1.09E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC5(YDR128W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NAB6(YML117W)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NFI1(YOR156C)|FD-Score:5.85|P-value:2.45E-9||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NRT1(YOR071C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OCT1(YKL134C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI7(YDR360W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PDA1(YER178W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PHO81(YGR233C)|FD-Score:5.57|P-value:1.31E-8||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIF1(YML061C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PTC1(YDL006W)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAV1(YJR033C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RLF2(YPR018W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RTC6(YPL183W-A)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTF1(YGL244W)|FD-Score:6.84|P-value:3.86E-12||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT10(YPL183C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SCP1(YOR367W)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SDC1(YDR469W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SHE3(YBR130C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SOV1(YMR066W)|FD-Score:5.75|P-value:4.49E-9||SGD DESC:Mitochondrial protein of unknown function Gene:SWI4(YER111C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYS1(YJL004C)|FD-Score:9.26|P-value:9.99E-21||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TCO89(YPL180W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TIP41(YPR040W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:7.19|P-value:3.28E-13||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.25|P-value:2.07E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS65(YLR322W_d)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCL042W(YCL042W_p)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:5.64|P-value:8.27E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR467C(YDR467C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR034C(YMR034C_p)|FD-Score:-5.31|P-value:5.51E-8||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL285W(YNL285W_d)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR014C(YPR014C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:5.78|P-value:3.65E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ADO1(YJR105W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM18(YHR198C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALE1(YOR175C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ANT1(YPR128C)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APT2(YDR441C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ARL1(YBR164C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO3(YDR035W)|FD-Score:6.26|P-value:1.87E-10||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG13(YPR185W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:BAT2(YJR148W)|FD-Score:-4.03|P-value:2.75E-5||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BTS1(YPL069C)|FD-Score:8.83|P-value:5.24E-19||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DIG2(YDR480W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOT6(YER088C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:FLC2(YAL053W)|FD-Score:4.27|P-value:9.67E-6||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP16(YDR070C_p)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FUN26(YAL022C)|FD-Score:-4.48|P-value:3.69E-6||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GCN2(YDR283C)|FD-Score:5.29|P-value:6.09E-8||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.15|P-value:1.27E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP3(YOR205C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GND2(YGR256W)|FD-Score:4.46|P-value:4.14E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GPG1(YGL121C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HCR1(YLR192C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IRA2(YOL081W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MGA2(YIR033W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MIC23(YBL107C_p)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MRC1(YCL061C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL22(YNL177C)|FD-Score:5.6|P-value:1.09E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC5(YDR128W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NAB6(YML117W)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NFI1(YOR156C)|FD-Score:5.85|P-value:2.45E-9||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NRT1(YOR071C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OCT1(YKL134C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI7(YDR360W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PDA1(YER178W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PHO81(YGR233C)|FD-Score:5.57|P-value:1.31E-8||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIF1(YML061C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PTC1(YDL006W)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAV1(YJR033C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RLF2(YPR018W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RTC6(YPL183W-A)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTF1(YGL244W)|FD-Score:6.84|P-value:3.86E-12||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT10(YPL183C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SCP1(YOR367W)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SDC1(YDR469W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SHE3(YBR130C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SOV1(YMR066W)|FD-Score:5.75|P-value:4.49E-9||SGD DESC:Mitochondrial protein of unknown function Gene:SWI4(YER111C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYS1(YJL004C)|FD-Score:9.26|P-value:9.99E-21||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TCO89(YPL180W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TIP41(YPR040W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:7.19|P-value:3.28E-13||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.25|P-value:2.07E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS65(YLR322W_d)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCL042W(YCL042W_p)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:5.64|P-value:8.27E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR467C(YDR467C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR034C(YMR034C_p)|FD-Score:-5.31|P-value:5.51E-8||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL285W(YNL285W_d)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR014C(YPR014C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:5.78|P-value:3.65E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR156W5.101.66E-71.28PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YLR208W4.769.67E-71.28SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YLR060W4.582.28E-61.28FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YMR260C3.314.71E-40.20TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YMR208W3.119.36E-40.03ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YER165W3.080.001040.02PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YDR037W3.060.001120.23KRS1Lysyl-tRNA synthetase
YFL039C2.820.002370.02ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YKL035W2.800.002540.07UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YOL139C2.730.003130.04CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YJL032W_d2.700.003500.01YJL032W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
YNL006W2.690.003590.15LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
YOR224C2.530.005640.03RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YPL094C2.500.006160.07SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YDR164C2.440.007390.03SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL004C9.269.99E-21SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YPL069C8.835.24E-19BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDR007W7.193.28E-13TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YGL244W6.843.86E-12RTF1Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
YDR035W6.261.87E-10ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YKL211C6.252.07E-10TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YOR156C5.852.45E-9NFI1SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length
YPR123C_d5.783.65E-9YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YMR066W5.754.49E-9SOV1Mitochondrial protein of unknown function
YDR008C_d5.648.27E-9YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNL177C5.601.09E-8MRPL22Mitochondrial ribosomal protein of the large subunit
YGR233C5.571.31E-8PHO81Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress
YDR283C5.296.09E-8GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YEL009C5.151.27E-7GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YER090W4.865.99E-7TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p

GO enrichment analysis for SGTC_727
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3052.53E-127SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0864198
0.2959.56E-119SGTC_24885268135 45.7 μMMiscellaneous12703560.0909091
0.2885.38E-113SGTC_18825615643 20.0 μMMiscellaneous22530750.0843373TRP & mitochondrial translation
0.2871.12E-112SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0714286
0.2857.63E-111SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0108696
0.2859.04E-111SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0508475
0.2843.70E-110SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.04
0.2757.62E-103SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.134328
0.2723.06E-100SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.04
0.2671.26E-96SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.140625
0.2661.65E-96SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0967742TRP & mitochondrial translation
0.2651.58E-95SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.0882353
0.2645.66E-95SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0875
0.2646.85E-95SGTC_18815652484 16.0 μMMiscellaneous22551290.0595238TRP & mitochondrial translation
0.2637.24E-94SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.123077

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_420133-008660.65 μM0.314815162923ChemDiv (Drug-like library)310.573663.912
SGTC_3194911190349.47 μM0.30508517087251Chembridge (Drug-like library)303.740263.71313
SGTC_15024534-428227.7 μM0.3015873278949ChemDiv (Drug-like library)305.716382.16415
SGTC_3474534-131126.2 μM0.2727272862016ChemDiv (Drug-like library)348.780883.05315RPP1 & pyrimidine depletion
SGTC_8650488-0499301 μM0.2678579634651ChemDiv (Drug-like library)203.200661.58426
SGTC_2879904181571.43 μM0.2622954942971Chembridge (Drug-like library)289.756744.13512
SGTC_3278913960549.47 μM0.2586217958859Chembridge (Drug-like library)267.6585263.6913
SGTC_2832799276251.95 μM0.2580652985177Chembridge (Drug-like library)299.71183.75204
SGTC_3031908876249.47 μM0.254237205253Chembridge (Drug-like library)349.254324.11302
SGTC_8780828-028913.7 μM0.25745350ChemDiv (Drug-like library)298.720443.90303