3030-5151

7-methyl-2-(3-phenylmethoxyphenyl)-1,2,3,5,6,8-hexahydropyrido[2,3]thieno[2,4-d]pyrimidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_728
Screen concentration 22.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 3125388
SMILES CN1CCC2=C(C1)SC3=C2C(=O)NC(N3)C4=CC(=CC=C4)OCC5=CC=CC=C5
Standardized SMILES CN1CCc2c(C1)sc3NC(NC(=O)c23)c4cccc(OCc5ccccc5)c4
Molecular weight 405.5126
ALogP 4.03
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.53
% growth inhibition (Hom. pool) 2.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3125388
Download HIP data (tab-delimited text)  (excel)
Gene:ERG1(YGR175C)|FD-Score:3.8|P-value:7.34E-5|Clearance:0.44||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:NMD3(YHR170W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.44||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PDS1(YDR113C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.36||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP21(YJL203W)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.44||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:SAS10(YDL153C)|FD-Score:4.33|P-value:7.61E-6|Clearance:0.44||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:ERG1(YGR175C)|FD-Score:3.8|P-value:7.34E-5|Clearance:0.44||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:NMD3(YHR170W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.44||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PDS1(YDR113C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.36||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP21(YJL203W)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.44||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:SAS10(YDL153C)|FD-Score:4.33|P-value:7.61E-6|Clearance:0.44||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3125388
Download HOP data (tab-delimited text)  (excel)
Gene:ALG6(YOR002W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APM1(YPL259C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:BLS1(YLR408C_p)|FD-Score:-4.85|P-value:6.30E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BNA5(YLR231C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CAT8(YMR280C)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CNA1(YLR433C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:DOT6(YER088C)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DSK2(YMR276W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EDE1(YBL047C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELP6(YMR312W)|FD-Score:5.58|P-value:1.17E-8||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FMP43(YGR243W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GBP2(YCL011C)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GRX3(YDR098C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:IML2(YJL082W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC21(YMR073C)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JJJ1(YNL227C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:KAR4(YCL055W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KCC4(YCL024W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:LYS21(YDL131W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MDV1(YJL112W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MET22(YOL064C)|FD-Score:5.21|P-value:9.70E-8||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MRP49(YKL167C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:PMT2(YAL023C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:REI1(YBR267W)|FD-Score:5.56|P-value:1.32E-8||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGM1(YMR182C)|FD-Score:4.39|P-value:5.59E-6||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:6.35|P-value:1.06E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:SBE22(YHR103W)|FD-Score:-5.74|P-value:4.76E-9||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SMI1(YGR229C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:STM1(YLR150W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:YDR442W(YDR442W_d)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL067C(YIL067C_p)|FD-Score:5.41|P-value:3.23E-8||SGD DESC:Uncharacterized protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:-4.57|P-value:2.41E-6||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL195C(YNL195C_p)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL085C(YOL085C_d)|FD-Score:4.45|P-value:4.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR052C(YOR052C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR059C(YOR059C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YPL229W(YPL229W_p)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR089W(YPR089W_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:ALG6(YOR002W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APM1(YPL259C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:BLS1(YLR408C_p)|FD-Score:-4.85|P-value:6.30E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BNA5(YLR231C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CAT8(YMR280C)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CNA1(YLR433C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:DOT6(YER088C)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DSK2(YMR276W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EDE1(YBL047C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELP6(YMR312W)|FD-Score:5.58|P-value:1.17E-8||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FMP43(YGR243W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GBP2(YCL011C)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GRX3(YDR098C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Gene:IML2(YJL082W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC21(YMR073C)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JJJ1(YNL227C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:KAR4(YCL055W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KCC4(YCL024W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:LYS21(YDL131W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MDV1(YJL112W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MET22(YOL064C)|FD-Score:5.21|P-value:9.70E-8||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MRP49(YKL167C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:PMT2(YAL023C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:REI1(YBR267W)|FD-Score:5.56|P-value:1.32E-8||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGM1(YMR182C)|FD-Score:4.39|P-value:5.59E-6||SGD DESC:Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:6.35|P-value:1.06E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:SBE22(YHR103W)|FD-Score:-5.74|P-value:4.76E-9||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SMI1(YGR229C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:STM1(YLR150W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:YDR442W(YDR442W_d)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL067C(YIL067C_p)|FD-Score:5.41|P-value:3.23E-8||SGD DESC:Uncharacterized protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:-4.57|P-value:2.41E-6||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL195C(YNL195C_p)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL085C(YOL085C_d)|FD-Score:4.45|P-value:4.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR052C(YOR052C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR059C(YOR059C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YPL229W(YPL229W_p)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR089W(YPR089W_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL153C4.337.61E-60.44SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YHR170W4.211.25E-50.44NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGR175C3.807.34E-50.44ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YJL203W3.591.66E-40.44PRP21Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YDR113C3.148.32E-40.36PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YPL142C_d2.790.002650.00YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YOL146W2.790.002660.02PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YMR093W2.770.002820.07UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YPR161C2.700.003480.02SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YAR019C2.680.003710.12CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YNL251C2.550.005330.01NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YBR091C2.540.005470.06TIM12Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
YGR172C2.480.006560.01YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YOR254C2.470.006760.11SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YIL150C2.360.009050.01MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL090C6.351.06E-10RPS6AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
YMR312W5.581.17E-8ELP6Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
YBR267W5.561.32E-8REI1Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
YIL067C_p5.413.23E-8YIL067C_pUncharacterized protein of unknown function
YOL064C5.219.70E-8MET22Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway
YMR073C4.953.69E-7IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YGR229C4.691.38E-6SMI1Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
YOR323C4.661.61E-6PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YOL085C_d4.454.28E-6YOL085C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A
YMR182C4.395.59E-6RGM1Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication
YLR231C4.052.54E-5BNA5Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YOR002W4.013.06E-5ALG6Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
YLR433C3.973.65E-5CNA1Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
YNL195C_p3.914.68E-5YNL195C_pPutative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YIL002C3.904.80E-5INP51Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth

GO enrichment analysis for SGTC_728
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0992.59E-14SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.045977
0.0849.07E-11SGTC_14073909-7909 12.3 μMChemDiv (Drug-like library)44117800.094736860S ribosome export
0.0757.64E-9SGTC_14924158-0558 28.5 μMChemDiv (Drug-like library)39672680.13978560S ribosome export
0.0732.43E-8SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.12359660S ribosome export
0.0691.12E-7SGTC_22697935389 174.4 μMChembridge (Fragment library)976050.0853659tubulin folding & SWR complex
0.0681.38E-7SGTC_7294092-0585 94.1 μMChemDiv (Drug-like library)21690610.211765
0.0672.61E-7SGTC_30879117406 49.5 μMChembridge (Drug-like library)425460930.108911
0.0673.05E-7SGTC_7313966-0296 387.0 μMChemDiv (Drug-like library)6609560.147727
0.0663.39E-7SGTC_9483448-0295 75.3 μMChemDiv (Drug-like library)6785690.0588235
0.0664.21E-7SGTC_9031134-0042 114.0 μMChemDiv (Drug-like library)7010090.0759494
0.0649.19E-7SGTC_24675474540 200.0 μMMiscellaneous54135190.0777778
0.0631.23E-6SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.13636460S ribosome export
0.0622.03E-6SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.10784360S ribosome export
0.0622.19E-6SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.10784360S ribosome export
0.0612.76E-6SGTC_1729st035615 46.0 μMTimTec (Natural product derivative library)173854510.130952

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1613st00238152.1 μM0.304878551167TimTec (Natural product derivative library)363.45283.83913
SGTC_2790542397071.43 μM0.2948721376240Chembridge (Drug-like library)389.486785.09604endomembrane recycling
SGTC_2697mebhydrolin72.4 μM0.26666722530TimTec (Natural product derivative library)276.37554.06601NEO1
SGTC_13893405-018719 μM0.256757799890ChemDiv (Drug-like library)311.441145.15102
SGTC_1801st05008928.44 μM0.2560985008493TimTec (Natural product derivative library)389.486785.27914NEO1-PIK1
SGTC_309k408-00511.48 μM0.2446813640463ChemDiv (Drug-like library)505.650043.32218
SGTC_24045161160200 μM0.2439022831543Miscellaneous437.285984.61714
SGTC_2868903560832.47 μM0.2409646462614Chembridge (Drug-like library)366.238483.82703
SGTC_2007401153971.82 μM0.23287745595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_5564058-006351.5 μM0.2317076615692ChemDiv (Drug-like library)417.276424.07924