1436-0052

1-(4-fluorophenyl)-3-(2-hydroxy-5-methoxyphenyl)propane-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_730
Screen concentration 41.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 768764
SMILES COC1=CC(=C(C=C1)O)C(=O)CC(=O)C2=CC=C(C=C2)F
Standardized SMILES COc1ccc(O)c(c1)C(=O)CC(=O)c2ccc(F)cc2
Molecular weight 288.2704
ALogP 3.1
H-bond donor count 1
H-bond acceptor count 5
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 31.16
% growth inhibition (Hom. pool) 7.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 768764
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.05||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT3(YJL014W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDS1(YBR029C)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CLP1(YOR250C)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DBP2(YNL112W)|FD-Score:5.61|P-value:1.02E-8|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DIM1(YPL266W)|FD-Score:16.9|P-value:2.60E-64|Clearance:6.09||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0.08||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FOL3(YMR113W)|FD-Score:14.4|P-value:2.97E-47|Clearance:6.09||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HEM12(YDR047W)|FD-Score:6.18|P-value:3.24E-10|Clearance:0.49||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:5.08|P-value:1.92E-7|Clearance:0.28||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:ILV5(YLR355C)|FD-Score:5.69|P-value:6.24E-9|Clearance:0.09||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IMP3(YHR148W)|FD-Score:-3.83|P-value:6.49E-5|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MET30(YIL046W)|FD-Score:5.31|P-value:5.53E-8|Clearance:0.15||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP8(YOL144W)|FD-Score:12.3|P-value:7.09E-35|Clearance:6.09||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PKC1(YBL105C)|FD-Score:5.58|P-value:1.22E-8|Clearance:0.27||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE10(YOR362C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RIM2(YBR192W)|FD-Score:15.1|P-value:7.28E-52|Clearance:6.09||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL25(YOL127W)|FD-Score:3.89|P-value:4.99E-5|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:SEC63(YOR254C)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.3||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC98(YNL126W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.1||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPN1(YPR133C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.18||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SQT1(YIR012W)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.16||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SRB4(YER022W)|FD-Score:5.61|P-value:1.03E-8|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TUB4(YLR212C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.08||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:4.8|P-value:7.91E-7|Clearance:0.56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:BET4(YJL031C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.05||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT3(YJL014W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDS1(YBR029C)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CLP1(YOR250C)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DBP2(YNL112W)|FD-Score:5.61|P-value:1.02E-8|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DIM1(YPL266W)|FD-Score:16.9|P-value:2.60E-64|Clearance:6.09||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0.08||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FOL3(YMR113W)|FD-Score:14.4|P-value:2.97E-47|Clearance:6.09||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HEM12(YDR047W)|FD-Score:6.18|P-value:3.24E-10|Clearance:0.49||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:5.08|P-value:1.92E-7|Clearance:0.28||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:ILV5(YLR355C)|FD-Score:5.69|P-value:6.24E-9|Clearance:0.09||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IMP3(YHR148W)|FD-Score:-3.83|P-value:6.49E-5|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MET30(YIL046W)|FD-Score:5.31|P-value:5.53E-8|Clearance:0.15||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NOP8(YOL144W)|FD-Score:12.3|P-value:7.09E-35|Clearance:6.09||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PKC1(YBL105C)|FD-Score:5.58|P-value:1.22E-8|Clearance:0.27||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE10(YOR362C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RIM2(YBR192W)|FD-Score:15.1|P-value:7.28E-52|Clearance:6.09||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL25(YOL127W)|FD-Score:3.89|P-value:4.99E-5|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:SEC63(YOR254C)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.3||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC98(YNL126W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.1||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPN1(YPR133C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.18||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SQT1(YIR012W)|FD-Score:3.26|P-value:5.57E-4|Clearance:0.16||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SRB4(YER022W)|FD-Score:5.61|P-value:1.03E-8|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TUB4(YLR212C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.08||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:4.8|P-value:7.91E-7|Clearance:0.56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 768764
Download HOP data (tab-delimited text)  (excel)
Gene:ARG82(YDR173C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:AST2(YER101C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BNA7(YDR428C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BRE2(YLR015W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BSC4(YNL269W)|FD-Score:-3.91|P-value:4.58E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BUB2(YMR055C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CKA2(YOR061W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CNN1(YFR046C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:DAS2(YDR020C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DSD1(YGL196W)|FD-Score:-5.02|P-value:2.53E-7||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EGD1(YPL037C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ERG5(YMR015C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:IRC21(YMR073C)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISR1(YPR106W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:JJJ1(YNL227C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:LAS21(YJL062W)|FD-Score:4.83|P-value:6.72E-7||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MAC1(YMR021C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET14(YKL001C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MET22(YOL064C)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MTC1(YJL123C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:NNF2(YGR089W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUR1(YDL089W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PEP3(YLR148W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PUT2(YHR037W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD61(YDR014W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RMD8(YFR048W)|FD-Score:5.4|P-value:3.36E-8||SGD DESC:Cytosolic protein required for sporulation Gene:RPS10B(YMR230W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SPO20(YMR017W)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SPR6(YER115C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPS1(YDR523C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis Gene:SPS19(YNL202W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SSN2(YDR443C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TUL1(YKL034W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:VAC7(YNL054W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YAP3(YHL009C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBR071W(YBR071W)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YCF1(YDR135C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YER091C-A(YER091C-A_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR242W(YGR242W_d)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR210C(YHR210C_p)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YJL007C(YJL007C_d)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:4.78|P-value:8.74E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR407W(YLR407W_p)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR034C(YMR034C_p)|FD-Score:-4.01|P-value:3.01E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNR014W(YNR014W_p)|FD-Score:-3.72|P-value:9.87E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YPL067C(YPL067C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL068C(YPL068C_p)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:ARG82(YDR173C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:AST2(YER101C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BNA7(YDR428C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BRE2(YLR015W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BSC4(YNL269W)|FD-Score:-3.91|P-value:4.58E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BUB2(YMR055C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CKA2(YOR061W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CNN1(YFR046C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:DAS2(YDR020C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DSD1(YGL196W)|FD-Score:-5.02|P-value:2.53E-7||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EGD1(YPL037C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ERG5(YMR015C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:IRC21(YMR073C)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISR1(YPR106W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:JJJ1(YNL227C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:LAS21(YJL062W)|FD-Score:4.83|P-value:6.72E-7||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MAC1(YMR021C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET14(YKL001C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MET22(YOL064C)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MTC1(YJL123C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:NNF2(YGR089W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NUR1(YDL089W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PEP3(YLR148W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PUT2(YHR037W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD61(YDR014W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RMD8(YFR048W)|FD-Score:5.4|P-value:3.36E-8||SGD DESC:Cytosolic protein required for sporulation Gene:RPS10B(YMR230W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SPO20(YMR017W)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SPR6(YER115C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPS1(YDR523C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis Gene:SPS19(YNL202W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SSN2(YDR443C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TUL1(YKL034W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:VAC7(YNL054W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YAP3(YHL009C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBR071W(YBR071W)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YCF1(YDR135C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YER091C-A(YER091C-A_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR242W(YGR242W_d)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR210C(YHR210C_p)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YJL007C(YJL007C_d)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:4.78|P-value:8.74E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR407W(YLR407W_p)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR034C(YMR034C_p)|FD-Score:-4.01|P-value:3.01E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNR014W(YNR014W_p)|FD-Score:-3.72|P-value:9.87E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YPL067C(YPL067C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL068C(YPL068C_p)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL266W16.902.60E-646.09DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YBR192W15.107.28E-526.09RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YMR113W14.402.97E-476.09FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YOL144W12.307.09E-356.09NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YDR047W6.183.24E-100.48HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YLR355C5.696.24E-90.09ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YNL112W5.611.02E-85.35E-4DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YER022W5.611.03E-80.03SRB4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
YBL105C5.581.22E-80.27PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YIL046W5.315.53E-80.15MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YMR211W5.151.28E-70.08DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YDL205C5.081.92E-70.28HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YJL086C_d4.807.91E-70.56YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YDL196W_d4.241.13E-50.35YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YOL127W3.894.99E-50.09RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFR048W5.403.36E-8RMD8Cytosolic protein required for sporulation
YHR210C_p4.973.34E-7YHR210C_pPutative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions
YJL062W4.836.72E-7LAS21Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity
YLR374C_d4.788.74E-7YLR374C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
YKL034W4.731.10E-6TUL1Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
YPL068C_p4.572.46E-6YPL068C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS
YMR017W4.318.20E-6SPO20Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog
YGR242W_d4.279.58E-6YGR242W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
YNL227C4.231.19E-5JJJ1Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
YDR173C4.111.96E-5ARG82Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
YFR046C4.082.29E-5CNN1Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
YMR015C4.052.60E-5ERG5C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YLR015W3.924.42E-5BRE2Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
YAL067W-A_p3.885.16E-5YAL067W-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YLR148W3.885.19E-5PEP3Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis

GO enrichment analysis for SGTC_730
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4209.15E-251SGTC_24755812728 15.0 μMMiscellaneous7761600.510204mitochondrial processes
0.4076.16E-234SGTC_24715466956 24.8 μMMiscellaneous7244190.425532mitochondrial processes
0.3701.51E-190SGTC_24595811157 9.2 μMMiscellaneous28706840.458333mitochondrial processes
0.2229.20E-67SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0416667heme biosynthesis & mitochondrial translocase
0.2042.45E-56SGTC_21395578692 56.5 μMChembridge (Fragment library)6509910.0454545superoxide
0.1983.70E-53SGTC_630099-0242 26.7 μMChemDiv (Drug-like library)38985020.0677966mitochondrial stress
0.1824.59E-45SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.118644heme biosynthesis & mitochondrial translocase
0.1799.03E-44SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.153846mitochondrial processes
0.1667.77E-38SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.0869565heme biosynthesis & mitochondrial translocase
0.1631.88E-36SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.0857143mitochondrial processes
0.1604.54E-35SGTC_8510394-0702 453.0 μMChemDiv (Drug-like library)7626460.0606061mitochondrial processes
0.1532.28E-32SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0434783heme biosynthesis & mitochondrial translocase
0.1532.56E-32SGTC_24495812982 45.8 μMMiscellaneous7761850.48
0.1527.29E-32SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0666667heme biosynthesis & mitochondrial translocase
0.1511.72E-31SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.22807

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2477596768625.23 μM0.756098776118Miscellaneous270.279962.89614
SGTC_2481547894845.16 μM0.62790777792Miscellaneous270.279962.89614PDR1
SGTC_2475581272815.03 μM0.510204776160Miscellaneous272.2710233.60414mitochondrial processes
SGTC_2449581298245.77 μM0.48776185Miscellaneous292.6895033.78214
SGTC_245958111579.22 μM0.4583332870684Miscellaneous258.2444433.11814mitochondrial processes
SGTC_2491paeonol100 μM0.43181811092Microsource (Natural product library)166.17391.31113
SGTC_2471546695624.79 μM0.425532724419Miscellaneous274.699043.57713mitochondrial processes
SGTC_24825981709142.65 μM0.42877755Miscellaneous333.176624.14713PDR1
SGTC_2610ononetin44.17 μM0.392157259632Microsource (Natural product library)258.269262.76824
SGTC_25792',5'-dihydroxy-4-methoxychalcone13.23 μM0.3773585355888Microsource (Natural product library)270.279963.20124