3970-1727

(5E)-5-[(2-ethoxynaphthalen-1-yl)methylidene]-1-(4-methoxyphenyl)-1,3-diazinane-2,4,6-trione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_732
Screen concentration 52.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 1275699
SMILES CCOC1=C(C2=CC=CC=C2C=C1)C=C3C(=O)NC(=O)N(C3=O)C4=CC=C(C=C4)OC
Standardized SMILES CCOc1ccc2ccccc2c1C=C3C(=O)NC(=O)N(C3=O)c4ccc(OC)cc4
Molecular weight 416.426
ALogP 3.87
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.16
% growth inhibition (Hom. pool) 10.13


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1275699
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:-3.36|P-value:3.90E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT3(YJL014W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.07||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG29(YMR134W_p)|FD-Score:5.7|P-value:6.16E-9|Clearance:1.49||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GLC7(YER133W)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.12||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NMD3(YHR170W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.27||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PSF3(YOL146W)|FD-Score:3.28|P-value:5.22E-4|Clearance:0.19||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPP1(YHR062C)|FD-Score:3.79|P-value:7.53E-5|Clearance:0.41||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC13(YLR208W)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.01||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPT5(YML010W)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.02||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:YGR114C(YGR114C_d)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ARP9(YMR033W)|FD-Score:-3.36|P-value:3.90E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT3(YJL014W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.07||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG29(YMR134W_p)|FD-Score:5.7|P-value:6.16E-9|Clearance:1.49||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GLC7(YER133W)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.12||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NMD3(YHR170W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.27||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PSF3(YOL146W)|FD-Score:3.28|P-value:5.22E-4|Clearance:0.19||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPP1(YHR062C)|FD-Score:3.79|P-value:7.53E-5|Clearance:0.41||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC13(YLR208W)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.01||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPT5(YML010W)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.02||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:YGR114C(YGR114C_d)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1275699
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AIM9(YER080W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AMA1(YGR225W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:BLS1(YLR408C_p)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:CTF4(YPR135W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DCW1(YKL046C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DIG1(YPL049C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DIG2(YDR480W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DPH1(YIL103W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM31(YBR176W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EHD3(YDR036C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:ERJ5(YFR041C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:EUG1(YDR518W)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:HHY1(YEL059W_d)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:MET22(YOL064C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MNT4(YNR059W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MPA43(YNL249C)|FD-Score:-3.78|P-value:7.73E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NFI1(YOR156C)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:OPT2(YPR194C)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PRS2(YER099C)|FD-Score:5.22|P-value:9.03E-8||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RLF2(YPR018W)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL29(YFR032C-A)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL34B(YIL052C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:SDS24(YBR214W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication Gene:SEE1(YIL064W)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SLM5(YCR024C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SRO77(YBL106C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:TDA11(YHR159W_p)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:UBP9(YER098W)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:YBR053C(YBR053C_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YCL042W(YCL042W_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR108C(YLR108C_p)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YMR034C(YMR034C_p)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL046W(YNL046W_p)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YNL195C(YNL195C_p)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL085C(YOL085C_d)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR082C(YOR082C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR238W(YOR238W_p)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPK1(YKL126W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:11.7|P-value:9.28E-32||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ACM1(YPL267W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AIM9(YER080W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AMA1(YGR225W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:BLS1(YLR408C_p)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:CTF4(YPR135W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DCW1(YKL046C)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DIG1(YPL049C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DIG2(YDR480W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DPH1(YIL103W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM31(YBR176W)|FD-Score:-3.13|P-value:8.85E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:EHD3(YDR036C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:ERJ5(YFR041C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:EUG1(YDR518W)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:HHY1(YEL059W_d)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:MET22(YOL064C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MNT4(YNR059W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MPA43(YNL249C)|FD-Score:-3.78|P-value:7.73E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NFI1(YOR156C)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:OPT2(YPR194C)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PRS2(YER099C)|FD-Score:5.22|P-value:9.03E-8||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:RLF2(YPR018W)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL29(YFR032C-A)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL34B(YIL052C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:SDS24(YBR214W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication Gene:SEE1(YIL064W)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SLM5(YCR024C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SRO77(YBL106C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:TDA11(YHR159W_p)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:UBP9(YER098W)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:YBR053C(YBR053C_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YCL042W(YCL042W_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR108C(YLR108C_p)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YMR034C(YMR034C_p)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YNL046W(YNL046W_p)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YNL195C(YNL195C_p)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL085C(YOL085C_d)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR082C(YOR082C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR238W(YOR238W_p)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPK1(YKL126W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:11.7|P-value:9.28E-32||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR134W_p5.706.16E-91.49ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YGR114C_d4.211.29E-50.03YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YHR170W4.181.45E-50.28NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YER133W3.914.69E-50.12GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YHR062C3.797.53E-50.41RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJL014W3.383.59E-40.07CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR208W3.314.67E-40.01SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YML010W3.304.88E-40.02SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YOL146W3.285.22E-40.19PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YDL195W3.099.93E-40.06SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YJL111W3.030.001210.04CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YMR093W2.990.001370.00UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YBL014C2.990.001390.06RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YAL034C-B_d2.930.001710.05YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL243W2.880.002020.08SRP68Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR262C11.709.28E-32YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YER099C5.229.03E-8PRS25-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
YLR408C_p4.904.77E-7BLS1_pPutative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
YER098W4.875.47E-7UBP9Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
YDR518W4.552.73E-6EUG1Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
YHR159W_p4.327.93E-6TDA11_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
YIL064W4.289.23E-6SEE1Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
YLR108C_p3.934.18E-5YLR108C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
YCR024C3.924.40E-5SLM5Mitochondrial asparaginyl-tRNA synthetase
YPR194C3.885.30E-5OPT2Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles
YFR032C-A3.797.53E-5RPL29Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
YKL046C3.797.67E-5DCW1Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p
YOL064C3.711.02E-4MET22Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway
YPR135W3.492.40E-4CTF4Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
YOR082C_d3.472.58E-4YOR082C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W

GO enrichment analysis for SGTC_732
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1611.63E-35SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.11236RPP1 & pyrimidine depletion
0.1292.08E-23SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.107143
0.1271.38E-22SGTC_162k030-0015 37.8 μMChemDiv (Drug-like library)18099200.1iron homeostasis
0.1181.00E-19SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0864198
0.1156.64E-19SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0740741
0.1095.62E-17SGTC_12670828-0272 9.5 μMChemDiv (Drug-like library)7453340.0740741
0.1062.72E-16SGTC_7560508-1999 28.3 μMChemDiv (Drug-like library)67538530.0888889RPP1 & pyrimidine depletion
0.1056.44E-16SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.315789
0.1049.11E-16SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.0952381
0.1023.25E-15SGTC_7161683-5115 658.0 μMChemDiv (Drug-like library)7235970.123457
0.1016.61E-15SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.141026
0.0992.09E-14SGTC_482fipronil 114.0 μMICCB bioactive library33520.0322581
0.0944.06E-13SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.0886076
0.0945.23E-13SGTC_410478-0632 82.6 μMChemDiv (Drug-like library)37504240.0769231
0.0921.28E-12SGTC_2811672-3440 14.9 μMChemDiv (Drug-like library)68330000.0537634Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7263448-439134.2 μM0.5873021356692ChemDiv (Drug-like library)388.439044.43814
SGTC_22037227154166.4 μM0.344262896304Chembridge (Fragment library)202.249062.46612
SGTC_9113456-4043236 μM0.342857914281ChemDiv (Drug-like library)280.321143.06312mitochondrial processes
SGTC_5991935-005286.5 μM0.3333339557311ChemDiv (Drug-like library)342.388982.07115
SGTC_1994st07294740.9 μM0.3157895286565TimTec (Natural product derivative library)397.44914.74823
SGTC_22236903751107.57 μM0.3055565598863Chembridge (Fragment library)299.2566032.23724superoxide
SGTC_1776st04924945.9 μM0.2820511822314TimTec (Natural product derivative library)435.473944.73613
SGTC_5602332-1943155 μM0.27710821177716ChemDiv (Drug-like library)403.430543.67314
SGTC_5491661-1316519 μM0.2643682857282ChemDiv (Drug-like library)385.455024.90324
SGTC_23207373003200 μM0.2631585415828Chembridge (Fragment library)291.325621.29425