4069-0007

3,5-dichloro-N-[(Z)-[4-(dimethylamino)phenyl]methylideneamino]pyridin-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_733
Screen concentration 56.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 5717425
SMILES CN(C)C1=CC=C(C=C1)C=NNC2=C(C=C(C=N2)Cl)Cl
Standardized SMILES CN(C)c1ccc(C=NNc2ncc(Cl)cc2Cl)cc1
Molecular weight 309.1938
ALogP 4.33
H-bond donor count 1
H-bond acceptor count 4
Response signature ERAD & cell cycle

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.47
% growth inhibition (Hom. pool) 21.05


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5717425
Download HIP data (tab-delimited text)  (excel)
Gene:CDC20(YGL116W)|FD-Score:5.47|P-value:2.20E-8|Clearance:0.09||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC23(YHR166C)|FD-Score:-4.29|P-value:8.97E-6|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC39(YCR093W)|FD-Score:7.25|P-value:2.09E-13|Clearance:0.89||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:ERG11(YHR007C)|FD-Score:8.29|P-value:5.85E-17|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS1(YKL182W)|FD-Score:3.38|P-value:3.69E-4|Clearance:0.05||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HSH49(YOR319W)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.25||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRA1(YBR140C)|FD-Score:5.35|P-value:4.50E-8|Clearance:0.07||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:IRR1(YIL026C)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.02||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MGE1(YOR232W)|FD-Score:9.13|P-value:3.43E-20|Clearance:0.84||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NFS1(YCL017C)|FD-Score:-4.64|P-value:1.76E-6|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:OLE1(YGL055W)|FD-Score:7.93|P-value:1.09E-15|Clearance:0.68||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PDC2(YDR081C)|FD-Score:4.87|P-value:5.63E-7|Clearance:0.29||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRP24(YMR268C)|FD-Score:5.28|P-value:6.52E-8|Clearance:0.39||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RET2(YFR051C)|FD-Score:4.89|P-value:5.02E-7|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPL25(YOL127W)|FD-Score:8.28|P-value:5.92E-17|Clearance:0.35||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN11(YFR004W)|FD-Score:-6.33|P-value:1.27E-10|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RSC8(YFR037C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SAM50(YNL026W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.05||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC13(YLR208W)|FD-Score:4.47|P-value:3.82E-6|Clearance:0.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC21(YNL287W)|FD-Score:21.4|P-value:3.45E-102|Clearance:6.42||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.11||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC31(YDL195W)|FD-Score:4.87|P-value:5.48E-7|Clearance:0.01||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN1(YLR430W)|FD-Score:5.38|P-value:3.72E-8|Clearance:0.03||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SGV1(YPR161C)|FD-Score:3.83|P-value:6.38E-5|Clearance:0.15||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:STH1(YIL126W)|FD-Score:6.36|P-value:1.03E-10|Clearance:0.88||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFC6(YDR362C)|FD-Score:4.27|P-value:9.81E-6|Clearance:0.04||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIM17(YJL143W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.05||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM40(YMR203W)|FD-Score:15|P-value:3.05E-51|Clearance:5.88||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:3.92|P-value:4.36E-5|Clearance:0.09||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR526C(YDR526C_d)|FD-Score:4.58|P-value:2.33E-6|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:CDC20(YGL116W)|FD-Score:5.47|P-value:2.20E-8|Clearance:0.09||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC23(YHR166C)|FD-Score:-4.29|P-value:8.97E-6|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC39(YCR093W)|FD-Score:7.25|P-value:2.09E-13|Clearance:0.89||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:ERG11(YHR007C)|FD-Score:8.29|P-value:5.85E-17|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS1(YKL182W)|FD-Score:3.38|P-value:3.69E-4|Clearance:0.05||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HSH49(YOR319W)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.25||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRA1(YBR140C)|FD-Score:5.35|P-value:4.50E-8|Clearance:0.07||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:IRR1(YIL026C)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.02||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MGE1(YOR232W)|FD-Score:9.13|P-value:3.43E-20|Clearance:0.84||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NFS1(YCL017C)|FD-Score:-4.64|P-value:1.76E-6|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:OLE1(YGL055W)|FD-Score:7.93|P-value:1.09E-15|Clearance:0.68||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PDC2(YDR081C)|FD-Score:4.87|P-value:5.63E-7|Clearance:0.29||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRP24(YMR268C)|FD-Score:5.28|P-value:6.52E-8|Clearance:0.39||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RET2(YFR051C)|FD-Score:4.89|P-value:5.02E-7|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPL25(YOL127W)|FD-Score:8.28|P-value:5.92E-17|Clearance:0.35||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN11(YFR004W)|FD-Score:-6.33|P-value:1.27E-10|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RSC8(YFR037C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SAM50(YNL026W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.05||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC13(YLR208W)|FD-Score:4.47|P-value:3.82E-6|Clearance:0.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC21(YNL287W)|FD-Score:21.4|P-value:3.45E-102|Clearance:6.42||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.11||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC31(YDL195W)|FD-Score:4.87|P-value:5.48E-7|Clearance:0.01||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN1(YLR430W)|FD-Score:5.38|P-value:3.72E-8|Clearance:0.03||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SGV1(YPR161C)|FD-Score:3.83|P-value:6.38E-5|Clearance:0.15||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:STH1(YIL126W)|FD-Score:6.36|P-value:1.03E-10|Clearance:0.88||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFC6(YDR362C)|FD-Score:4.27|P-value:9.81E-6|Clearance:0.04||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIM17(YJL143W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.05||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM40(YMR203W)|FD-Score:15|P-value:3.05E-51|Clearance:5.88||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:3.92|P-value:4.36E-5|Clearance:0.09||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR526C(YDR526C_d)|FD-Score:4.58|P-value:2.33E-6|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5717425
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ASC1(YMR116C)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATP15(YPL271W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIO4(YNR057C)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BSD2(YBR290W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:BST1(YFL025C)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CBF1(YJR060W)|FD-Score:5.28|P-value:6.54E-8||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CBS2(YDR197W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CCR4(YAL021C)|FD-Score:7.41|P-value:6.42E-14||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CDC50(YCR094W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:3.87|P-value:5.33E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSG2(YBR036C)|FD-Score:9|P-value:1.14E-19||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSR1(YLR380W)|FD-Score:10.6|P-value:1.59E-26||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CUE1(YMR264W)|FD-Score:8.04|P-value:4.40E-16||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DAL2(YIR029W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP1(YPL170W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBF2(YGR092W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:ECM8(YBR076W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Non-essential protein of unknown function Gene:ECT1(YGR007W)|FD-Score:6.85|P-value:3.61E-12||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ENV10(YLR065C)|FD-Score:6.28|P-value:1.64E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV9(YOR246C)|FD-Score:23.6|P-value:8.01E-124||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FAR10(YLR238W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FAR11(YNL127W)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FAR3(YMR052W)|FD-Score:7.59|P-value:1.60E-14||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FAR7(YFR008W)|FD-Score:5.29|P-value:6.02E-8||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FAR8(YMR029C)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FCJ1(YKR016W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FET4(YMR319C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FUM1(YPL262W)|FD-Score:-3.78|P-value:7.99E-5||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAL83(YER027C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCN5(YGR252W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:9.06|P-value:6.33E-20||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDT1(YBR187W_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GET1(YGL020C)|FD-Score:9.55|P-value:6.42E-22||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GET2(YER083C)|FD-Score:7.99|P-value:6.59E-16||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:10.7|P-value:5.82E-27||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLO3(YER122C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GRR1(YJR090C)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTT2(YLL060C)|FD-Score:-4.82|P-value:7.36E-7||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GUP2(YPL189W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:GYP1(YOR070C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HHT1(YBR010W)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HIT1(YJR055W)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HST2(YPL015C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXK2(YGL253W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITC1(YGL133W)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:IZH2(YOL002C)|FD-Score:18.5|P-value:4.25E-77||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KIN4(YOR233W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:KRE2(YDR483W)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:-3.73|P-value:9.41E-5||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LOA1(YPR139C)|FD-Score:4.34|P-value:7.00E-6||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM1(YJL124C)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MND2(YIR025W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNL2(YLR057W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MON1(YGL124C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MPM1(YJL066C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRPS17(YMR188C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSN1(YOL116W)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NBP2(YDR162C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NEM1(YHR004C)|FD-Score:9.7|P-value:1.52E-22||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:4.78|P-value:8.59E-7||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUP2(YLR335W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:PAU23(YLR037C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEP7(YDR323C)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX1(YKL197C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PEX21(YGR239C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PGD1(YGL025C)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:10.1|P-value:1.70E-24||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PHR1(YOR386W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIM1(YBL022C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN2(YOR104W)|FD-Score:4.35|P-value:6.84E-6||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PKH1(YDR490C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:POP2(YNR052C)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC1(YDL006W)|FD-Score:6.66|P-value:1.40E-11||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTM1(YKL039W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:PUB1(YNL016W)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QCR8(YJL166W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RCY1(YJL204C)|FD-Score:13.3|P-value:2.21E-40||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO5(YNL180C)|FD-Score:5.38|P-value:3.72E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RIM101(YHL027W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:5.32|P-value:5.26E-8||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:7.02|P-value:1.13E-12||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:6.58|P-value:2.35E-11||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL17B(YJL177W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:15.3|P-value:4.19E-53||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS25A(YGR027C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RTF1(YGL244W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC3(YDR159W)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAC7(YDR389W)|FD-Score:9.34|P-value:4.68E-21||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEC22(YLR268W)|FD-Score:13.5|P-value:7.99E-42||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SNF3(YDL194W)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:10.5|P-value:2.62E-26||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:7.16|P-value:4.05E-13||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPO7(YAL009W)|FD-Score:6.79|P-value:5.71E-12||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT21(YMR179W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SRF1(YDL133W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSE1(YPL106C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSM4(YIL030C)|FD-Score:35.4|P-value:1.55E-274||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STB5(YHR178W)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SUR4(YLR372W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUV3(YPL029W)|FD-Score:-3.86|P-value:5.70E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SVF1(YDR346C)|FD-Score:7.45|P-value:4.67E-14||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SWA2(YDR320C)|FD-Score:4.58|P-value:2.38E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWC5(YBR231C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TIF2(YJL138C)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TIM8(YJR135W-A)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TOM7(YNL070W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRI1(YMR233W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM9(YML014W)|FD-Score:18|P-value:4.65E-73||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:5.68|P-value:6.84E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS33(YOR115C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS85(YDR108W)|FD-Score:7.48|P-value:3.69E-14||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBC7(YMR022W)|FD-Score:20.3|P-value:1.57E-91||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBX2(YML013W)|FD-Score:5.87|P-value:2.17E-9||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGX2(YDL169C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VAM7(YGL212W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS20(YMR077C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:6.56|P-value:2.69E-11||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS5(YOR069W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS63(YLR261C_d)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS64(YDR200C)|FD-Score:10.3|P-value:4.82E-25||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS74(YDR372C)|FD-Score:6.09|P-value:5.62E-10||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:VTS1(YOR359W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:WHI3(YNL197C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YDL041W(YDL041W_d)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR186C(YDR186C)|FD-Score:12.3|P-value:5.32E-35||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR199W(YDR199W_d)|FD-Score:6.05|P-value:7.39E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR455C(YDR455C_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR535C(YDR535C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER084W(YER084W_p)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YGR054W(YGR054W)|FD-Score:6.89|P-value:2.80E-12||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YGR122W(YGR122W)|FD-Score:5.27|P-value:6.83E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR228W(YGR228W_d)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YGR242W(YGR242W_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YGR266W(YGR266W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YGR273C(YGR273C_p)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YJL160C(YJL160C_p)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YKL136W(YKL136W_d)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLL007C(YLL007C_p)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR031W(YLR031W_p)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Putative protein of unknown function Gene:YLR111W(YLR111W_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:9.53|P-value:7.63E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML037C(YML037C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YML122C(YML122C_d)|FD-Score:-5.01|P-value:2.67E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR007W(YMR007W_d)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:6.24|P-value:2.15E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL146C-A(YNL146C-A_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL088W(YPL088W_p)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPT52(YKR014C)|FD-Score:6.57|P-value:2.57E-11||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ACB1(YGR037C)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ASC1(YMR116C)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATP15(YPL271W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIO4(YNR057C)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BSD2(YBR290W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:BST1(YFL025C)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CBF1(YJR060W)|FD-Score:5.28|P-value:6.54E-8||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CBS2(YDR197W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CCR4(YAL021C)|FD-Score:7.41|P-value:6.42E-14||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CDC50(YCR094W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:3.87|P-value:5.33E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSG2(YBR036C)|FD-Score:9|P-value:1.14E-19||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSR1(YLR380W)|FD-Score:10.6|P-value:1.59E-26||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CUE1(YMR264W)|FD-Score:8.04|P-value:4.40E-16||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DAL2(YIR029W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP1(YPL170W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBF2(YGR092W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:ECM8(YBR076W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Non-essential protein of unknown function Gene:ECT1(YGR007W)|FD-Score:6.85|P-value:3.61E-12||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ENV10(YLR065C)|FD-Score:6.28|P-value:1.64E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV9(YOR246C)|FD-Score:23.6|P-value:8.01E-124||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FAR10(YLR238W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FAR11(YNL127W)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FAR3(YMR052W)|FD-Score:7.59|P-value:1.60E-14||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FAR7(YFR008W)|FD-Score:5.29|P-value:6.02E-8||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FAR8(YMR029C)|FD-Score:5.92|P-value:1.60E-9||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FCJ1(YKR016W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FET4(YMR319C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FUM1(YPL262W)|FD-Score:-3.78|P-value:7.99E-5||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAL83(YER027C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCN5(YGR252W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:9.06|P-value:6.33E-20||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDT1(YBR187W_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GET1(YGL020C)|FD-Score:9.55|P-value:6.42E-22||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GET2(YER083C)|FD-Score:7.99|P-value:6.59E-16||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:10.7|P-value:5.82E-27||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLO3(YER122C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GRR1(YJR090C)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTT2(YLL060C)|FD-Score:-4.82|P-value:7.36E-7||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GUP2(YPL189W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:GYP1(YOR070C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HHT1(YBR010W)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HIT1(YJR055W)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HST2(YPL015C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXK2(YGL253W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITC1(YGL133W)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:IZH2(YOL002C)|FD-Score:18.5|P-value:4.25E-77||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KIN4(YOR233W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:KRE2(YDR483W)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:-3.73|P-value:9.41E-5||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LOA1(YPR139C)|FD-Score:4.34|P-value:7.00E-6||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM1(YJL124C)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MND2(YIR025W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNL2(YLR057W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MON1(YGL124C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MPM1(YJL066C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRPS17(YMR188C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSN1(YOL116W)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NBP2(YDR162C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NEM1(YHR004C)|FD-Score:9.7|P-value:1.52E-22||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:4.78|P-value:8.59E-7||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUP2(YLR335W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:PAU23(YLR037C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEP7(YDR323C)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX1(YKL197C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PEX21(YGR239C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PGD1(YGL025C)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:10.1|P-value:1.70E-24||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PHR1(YOR386W)|FD-Score:3.86|P-value:5.57E-5||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIM1(YBL022C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN2(YOR104W)|FD-Score:4.35|P-value:6.84E-6||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PKH1(YDR490C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:POP2(YNR052C)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC1(YDL006W)|FD-Score:6.66|P-value:1.40E-11||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTM1(YKL039W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:PUB1(YNL016W)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QCR8(YJL166W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RCY1(YJL204C)|FD-Score:13.3|P-value:2.21E-40||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO5(YNL180C)|FD-Score:5.38|P-value:3.72E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RIM101(YHL027W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:5.32|P-value:5.26E-8||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:7.02|P-value:1.13E-12||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:6.58|P-value:2.35E-11||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL17B(YJL177W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:15.3|P-value:4.19E-53||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS25A(YGR027C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RTF1(YGL244W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC3(YDR159W)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAC7(YDR389W)|FD-Score:9.34|P-value:4.68E-21||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEC22(YLR268W)|FD-Score:13.5|P-value:7.99E-42||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SNF3(YDL194W)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:10.5|P-value:2.62E-26||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:7.16|P-value:4.05E-13||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPO7(YAL009W)|FD-Score:6.79|P-value:5.71E-12||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT21(YMR179W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SRF1(YDL133W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSE1(YPL106C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSM4(YIL030C)|FD-Score:35.4|P-value:1.55E-274||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:STB5(YHR178W)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SUR4(YLR372W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUV3(YPL029W)|FD-Score:-3.86|P-value:5.70E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SVF1(YDR346C)|FD-Score:7.45|P-value:4.67E-14||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SWA2(YDR320C)|FD-Score:4.58|P-value:2.38E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWC5(YBR231C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TIF2(YJL138C)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TIM8(YJR135W-A)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TOM7(YNL070W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRI1(YMR233W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM9(YML014W)|FD-Score:18|P-value:4.65E-73||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:5.68|P-value:6.84E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS33(YOR115C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS85(YDR108W)|FD-Score:7.48|P-value:3.69E-14||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBC7(YMR022W)|FD-Score:20.3|P-value:1.57E-91||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBX2(YML013W)|FD-Score:5.87|P-value:2.17E-9||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGX2(YDL169C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VAM7(YGL212W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS20(YMR077C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:6.56|P-value:2.69E-11||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS5(YOR069W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS63(YLR261C_d)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS64(YDR200C)|FD-Score:10.3|P-value:4.82E-25||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS74(YDR372C)|FD-Score:6.09|P-value:5.62E-10||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:VTS1(YOR359W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:WHI3(YNL197C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YDL041W(YDL041W_d)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR186C(YDR186C)|FD-Score:12.3|P-value:5.32E-35||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR199W(YDR199W_d)|FD-Score:6.05|P-value:7.39E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR455C(YDR455C_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR535C(YDR535C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER084W(YER084W_p)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YGR054W(YGR054W)|FD-Score:6.89|P-value:2.80E-12||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YGR122W(YGR122W)|FD-Score:5.27|P-value:6.83E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR228W(YGR228W_d)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YGR242W(YGR242W_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YGR266W(YGR266W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YGR273C(YGR273C_p)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YJL160C(YJL160C_p)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YKL136W(YKL136W_d)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLL007C(YLL007C_p)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR031W(YLR031W_p)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Putative protein of unknown function Gene:YLR111W(YLR111W_d)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:9.53|P-value:7.63E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML037C(YML037C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YML122C(YML122C_d)|FD-Score:-5.01|P-value:2.67E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR007W(YMR007W_d)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:6.24|P-value:2.15E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL146C-A(YNL146C-A_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL088W(YPL088W_p)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPT52(YKR014C)|FD-Score:6.57|P-value:2.57E-11||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL287W21.403.45E-1026.42SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YMR203W15.003.05E-515.88TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YOR232W9.133.43E-200.84MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YHR007C8.295.85E-170.00ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YOL127W8.285.92E-170.35RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YGL055W7.931.09E-150.68OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YCR093W7.252.09E-130.89CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YIL126W6.361.03E-100.88STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YGL116W5.472.20E-80.09CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YLR430W5.383.72E-80.03SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YBR140C5.354.50E-80.07IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YMR268C5.286.52E-80.39PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YFR051C4.895.02E-70.02RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YDL195W4.875.48E-70.01SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YDR081C4.875.63E-70.29PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL030C35.401.55E-274SSM4Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
YOR246C23.608.01E-124ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YMR022W20.301.57E-91UBC7Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
YOL002C18.504.25E-77IZH2Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
YML014W18.004.65E-73TRM9tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
YDL020C15.304.19E-53RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR268W13.507.99E-42SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YJL204C13.302.21E-40RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YDR186C12.305.32E-35YDR186CPutative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YDL100C10.705.82E-27GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
YLR380W10.601.59E-26CSR1Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
YLR025W10.502.62E-26SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YDR200C10.304.82E-25VPS64Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
YBR106W10.101.70E-24PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YHR004C9.701.52E-22NEM1Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard

GO enrichment analysis for SGTC_733
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5800SGTC_384k007-0144 5.2 μMChemDiv (Drug-like library)68248860.157143ERAD & cell cycle
0.5760SGTC_3730844-0104 25.9 μMChemDiv (Drug-like library)53758040.112903ERAD & cell cycle
0.5570SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.190476ERAD & cell cycle
0.5140SGTC_1161k007-0610 110.0 μMChemDiv (Drug-like library)58438500.119403ERAD & cell cycle
0.4840SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.0952381ERAD & cell cycle
0.4153.17E-244SGTC_352k048-0007 5.1 μMChemDiv (Drug-like library)68119110.225806ERAD & cell cycle
0.3893.71E-212SGTC_4260988-0037 14.6 μMChemDiv (Drug-like library)53830080.171875ERAD & cell cycle
0.3856.20E-208SGTC_1030844-0104 51.8 μMChemDiv (Drug-like library)53758040.112903ERAD & cell cycle
0.3695.00E-190SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.267857
0.3632.55E-183SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.106061fatty acid desaturase (OLE1)
0.3332.32E-152SGTC_5681193-0097 7.5 μMChemDiv (Drug-like library)36461500.131148ERAD & cell cycle
0.3241.46E-144SGTC_23227413319 38.2 μMChembridge (Fragment library)18404000.163934ERAD & cell cycle
0.3083.52E-130SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0714286
0.2612.78E-92SGTC_64000l-0018 272.1 μMChemDiv (Drug-like library)68111190.101266ubiquinone biosynthesis & proteasome
0.2601.86E-91SGTC_18845633444 25.0 μMMiscellaneous57242390.164179redox potentiating

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_774k060-0086136 μM0.4259265911235ChemDiv (Drug-like library)280.324443.67815
SGTC_208k072-009812.37 μM0.3461545948901ChemDiv (Drug-like library)231.68092.96213heme biosynthesis & mitochondrial translocase
SGTC_7051124-0212310 μM0.3035719554485ChemDiv (Drug-like library)293.1484.01212
SGTC_8650488-0499301 μM0.3018879634651ChemDiv (Drug-like library)203.200661.58426
SGTC_24605552655174.22 μM0.2982465401989Miscellaneous259.6912.62513amide catabolism
SGTC_7981287-0182476 μM0.2923081649755ChemDiv (Drug-like library)420.301825.78704
SGTC_630099-024226.7 μM0.283898502ChemDiv (Drug-like library)265.372744.0501mitochondrial stress
SGTC_287k060-003929.6 μM0.2786895861974ChemDiv (Drug-like library)305.1624.83423
SGTC_13631636-006310.4 μM0.2711866072185ChemDiv (Drug-like library)344.815323.95914Golgi
SGTC_1165k072-027530.3 μM0.2678576153523ChemDiv (Drug-like library)211.262422.78413