3076-0345

3-[(2,3-dimethylcyclohexyl)amino]-1-(4-methylphenyl)pyrrolidine-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_735
Screen concentration 636.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2888990
SMILES CC1CCCC(C1C)NC2CC(=O)N(C2=O)C3=CC=C(C=C3)C
Standardized SMILES CC1CCCC(NC2CC(=O)N(C2=O)c3ccc(C)cc3)C1C
Molecular weight 314.4219
ALogP 3.4
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.42
% growth inhibition (Hom. pool) 12.22


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2888990
Download HIP data (tab-delimited text)  (excel)
Gene:CDC28(YBR160W)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.06||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:COF1(YLL050C)|FD-Score:-3.39|P-value:3.51E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:IRA1(YBR140C)|FD-Score:-3.87|P-value:5.34E-5|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LAS1(YKR063C)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:NOC4(YPR144C)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.1||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:RET1(YOR207C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.1||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC53(YDL150W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.08||SGD DESC:RNA polymerase III subunit C53 Gene:RPP0(YLR340W)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:8.87|P-value:3.62E-19|Clearance:4.92||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SDH3(YKL141W)|FD-Score:-3.39|P-value:3.43E-4|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SUI3(YPL237W)|FD-Score:3.11|P-value:9.23E-4|Clearance:0.04||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:YBR190W(YBR190W_d)|FD-Score:3.87|P-value:5.40E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL152W(YDL152W_d)|FD-Score:3.79|P-value:7.59E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR187C(YDR187C_d)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YPL238C(YPL238C_d)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:CDC28(YBR160W)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.06||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:COF1(YLL050C)|FD-Score:-3.39|P-value:3.51E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:IRA1(YBR140C)|FD-Score:-3.87|P-value:5.34E-5|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LAS1(YKR063C)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:NOC4(YPR144C)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.1||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:RET1(YOR207C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.1||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC53(YDL150W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.08||SGD DESC:RNA polymerase III subunit C53 Gene:RPP0(YLR340W)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:8.87|P-value:3.62E-19|Clearance:4.92||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SDH3(YKL141W)|FD-Score:-3.39|P-value:3.43E-4|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SUI3(YPL237W)|FD-Score:3.11|P-value:9.23E-4|Clearance:0.04||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:YBR190W(YBR190W_d)|FD-Score:3.87|P-value:5.40E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL152W(YDL152W_d)|FD-Score:3.79|P-value:7.59E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR187C(YDR187C_d)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YPL238C(YPL238C_d)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2888990
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE3(YGR204W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AEP2(YMR282C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM3(YBR108W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALD3(YMR169C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:BIT2(YBR270C_p)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBR1(YIL043C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCE1(YKL011C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:COA3(YJL062W-A)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:DBP3(YGL078C)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DSS1(YMR287C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM30(YLR436C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:EFG1(YGR271C-A)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELP4(YPL101W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ELP6(YMR312W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FAA1(YOR317W)|FD-Score:4.94|P-value:3.81E-7||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FYV6(YNL133C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GID8(YMR135C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GPT2(YKR067W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GUT2(YIL155C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HSP26(YBR072W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HTD2(YHR067W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:IES5(YER092W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC3(YDR332W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KGD2(YDR148C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:LTE1(YAL024C)|FD-Score:5.87|P-value:2.21E-9||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS1(YIR034C)|FD-Score:-3.92|P-value:4.52E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:LYS9(YNR050C)|FD-Score:5.56|P-value:1.34E-8||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MRP51(YPL118W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL17(YNL252C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MSK1(YNL073W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MSN1(YOL116W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSS51(YLR203C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MUD1(YBR119W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:OCT1(YKL134C)|FD-Score:3.86|P-value:5.56E-5||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PDR16(YNL231C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX29(YDR479C)|FD-Score:5.87|P-value:2.16E-9||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFD1(YJL179W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PHO88(YBR106W)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POP2(YNR052C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RCO1(YMR075W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RLF2(YPR018W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RML2(YEL050C)|FD-Score:6.77|P-value:6.32E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RNP1(YLL046C)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:ROM2(YLR371W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL2B(YIL018W)|FD-Score:4.37|P-value:6.13E-6||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS19A(YOL121C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RPS9B(YBR189W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTR1(YER139C)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEA4(YBL104C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SMI1(YGR229C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNT309(YPR101W)|FD-Score:4.9|P-value:4.69E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:5.62|P-value:9.50E-9||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SRP40(YKR092C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:STF1(YDL130W-A)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:STO1(YMR125W)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUR4(YLR372W)|FD-Score:4.29|P-value:8.88E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA19(YKL056C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPS3(YMR261C)|FD-Score:6.07|P-value:6.40E-10||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRK2(YKR050W)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.41|P-value:3.10E-8||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YAL037W(YAL037W_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAR023C(YAR023C_p)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBR064W(YBR064W_d)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCR016W(YCR016W_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR049C(YCR049C_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL039W(YGL039W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YJR142W(YJR142W_p)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL169C(YKL169C_d)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLL056C(YLL056C_p)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR434C(YLR434C_d)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML122C(YML122C_d)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:4.93|P-value:4.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function Gene:YNL319W(YNL319W_d)|FD-Score:4|P-value:3.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR345C(YOR345C_d)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:ACO1(YLR304C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE3(YGR204W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AEP2(YMR282C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM3(YBR108W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALD3(YMR169C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:BIT2(YBR270C_p)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBR1(YIL043C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCE1(YKL011C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:COA3(YJL062W-A)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:DBP3(YGL078C)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DSS1(YMR287C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM30(YLR436C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:EFG1(YGR271C-A)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELP4(YPL101W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ELP6(YMR312W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FAA1(YOR317W)|FD-Score:4.94|P-value:3.81E-7||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FYV6(YNL133C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GID8(YMR135C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GPT2(YKR067W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GUT2(YIL155C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HSP26(YBR072W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:HTD2(YHR067W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:IES5(YER092W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC3(YDR332W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KGD2(YDR148C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:LTE1(YAL024C)|FD-Score:5.87|P-value:2.21E-9||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS1(YIR034C)|FD-Score:-3.92|P-value:4.52E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:LYS9(YNR050C)|FD-Score:5.56|P-value:1.34E-8||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MRP51(YPL118W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL17(YNL252C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MSK1(YNL073W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MSN1(YOL116W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSS51(YLR203C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MUD1(YBR119W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:OCT1(YKL134C)|FD-Score:3.86|P-value:5.56E-5||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PDR16(YNL231C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX29(YDR479C)|FD-Score:5.87|P-value:2.16E-9||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFD1(YJL179W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PHO88(YBR106W)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POP2(YNR052C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RCO1(YMR075W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p Gene:RLF2(YPR018W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RML2(YEL050C)|FD-Score:6.77|P-value:6.32E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RNP1(YLL046C)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:ROM2(YLR371W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL2B(YIL018W)|FD-Score:4.37|P-value:6.13E-6||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS19A(YOL121C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RPS9B(YBR189W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTR1(YER139C)|FD-Score:3.8|P-value:7.15E-5||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEA4(YBL104C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SMI1(YGR229C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNT309(YPR101W)|FD-Score:4.9|P-value:4.69E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:5.62|P-value:9.50E-9||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SRP40(YKR092C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:STF1(YDL130W-A)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:STO1(YMR125W)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUR4(YLR372W)|FD-Score:4.29|P-value:8.88E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA19(YKL056C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPS3(YMR261C)|FD-Score:6.07|P-value:6.40E-10||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRK2(YKR050W)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.41|P-value:3.10E-8||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:YAL037W(YAL037W_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAR023C(YAR023C_p)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBR064W(YBR064W_d)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCR016W(YCR016W_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR049C(YCR049C_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL039W(YGL039W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YJR142W(YJR142W_p)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL169C(YKL169C_d)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLL056C(YLL056C_p)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR434C(YLR434C_d)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML122C(YML122C_d)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:4.93|P-value:4.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function Gene:YNL319W(YNL319W_d)|FD-Score:4|P-value:3.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR345C(YOR345C_d)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C8.873.62E-194.92RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDL150W3.953.91E-50.08RPC53RNA polymerase III subunit C53
YBR190W_d3.875.40E-50.08YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YDL152W_d3.797.59E-50.14YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YOR207C3.641.35E-40.10RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YPL238C_d3.551.95E-40.01YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YPR144C3.532.05E-40.10NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YDR187C_d3.433.02E-40.26YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YBR160W3.177.52E-40.06CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YPL237W3.119.23E-40.04SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YGR119C3.070.001070.10NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YIL106W2.970.001490.00MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YDL147W2.970.001500.18RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YDR498C2.780.002680.12SEC20Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
YML031W2.660.003900.10NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL050C6.776.32E-12RML2Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
YMR261C6.076.40E-10TPS3Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
YDR479C5.872.16E-9PEX29Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
YAL024C5.872.21E-9LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YJR104C5.629.50E-9SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YNR050C5.561.34E-8LYS9Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
YMR125W5.472.30E-8STO1Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
YML124C5.413.10E-8TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YER097W_d5.161.24E-7YER097W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLL046C5.081.92E-7RNP1Ribonucleoprotein that contains two RNA recognition motifs (RRM)
YOR317W4.943.81E-7FAA1Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication
YNL043C_d4.934.04E-7YNL043C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
YPR101W4.904.69E-7SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
YOR345C_d4.885.35E-7YOR345C_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YLR287C-A4.671.53E-6RPS30AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_735
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1972.10E-52SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.25
0.1878.35E-48SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.1
0.1817.98E-45SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.128205
0.1791.00E-43SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.184615
0.1653.86E-37SGTC_503tyrphostin 8 294.0 μMICCB bioactive library929700.05TSC3-RPN4
0.1603.36E-35SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.381818
0.1575.59E-34SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0746269TSC3-RPN4
0.1553.45E-33SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.25TSC3-RPN4
0.1554.79E-33SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.12987
0.1512.37E-31SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0895522
0.1411.38E-27SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.0810811
0.1404.60E-27SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0895522TSC3-RPN4
0.1395.87E-27SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0571429TSC3-RPN4
0.1369.69E-26SGTC_5481436-0017 110.0 μMChemDiv (Drug-like library)96771370.0853659TSC3-RPN4
0.1351.76E-25SGTC_21615662504 8.5 μMChembridge (Fragment library)28593340.145161tubulin folding & SWR complex

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2474532157043.32 μM0.3818182841384Miscellaneous297.37153.55303
SGTC_5891227-007034.1 μM0.3818182841384ChemDiv (Drug-like library)297.37153.55303
SGTC_2469565501990.81 μM0.3548392788239Miscellaneous341.3813.18315
SGTC_21915911152200 μM0.3220342875902Chembridge (Fragment library)279.718861.92403
SGTC_1768st04430137.18 μM0.3026323931349TimTec (Natural product derivative library)419.516063.88514
SGTC_2123807-468238.77 μM0.2686573125501ChemDiv (Drug-like library)304.324381.35316tubulin folding & SWR complex
SGTC_6811642-000626.6 μM0.2686574446530ChemDiv (Drug-like library)461.599027.51215
SGTC_11791021-14319.29 μM0.2666672840958ChemDiv (Drug-like library)362.240983.81603
SGTC_21095483026200 μM0.264151759335Chembridge (Fragment library)190.19861.10812RSC complex & mRNA processing
SGTC_2465580924233.21 μM0.260872731748Miscellaneous357.38042.68116