k014-0057

N-[4-[(5-bromo-2-oxoindol-3-yl)amino]-2-nitrophenyl]-4-nitrobenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_740
Screen concentration 261.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4012779
SMILES C1=CC(=CC=C1C(=O)NC2=C(C=C(C=C2)NC3=C4C=C(C=CC4=NC3=O)Br)[N+](=O)[O-])[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(cc1)C(=O)Nc2ccc(cc2[N+](=O)[O-])N=C3C(=O)Nc4ccc(Br)cc34
Molecular weight 510.2539
ALogP 3.23
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.53
% growth inhibition (Hom. pool) 8.71


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4012779
Download HIP data (tab-delimited text)  (excel)
Gene:BRF1(YGR246C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.02||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:BRL1(YHR036W)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.04||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CSE4(YKL049C)|FD-Score:5.61|P-value:1.02E-8|Clearance:2.07||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:DHR2(YKL078W)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.04||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DIS3(YOL021C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ESP1(YGR098C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.01||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GPI16(YHR188C)|FD-Score:3.1|P-value:9.68E-4|Clearance:0.17||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:GPN2(YOR262W)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.01||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HRR25(YPL204W)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.02||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSP60(YLR259C)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.02||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:LSM5(YER146W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.02||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM6(YGL201C)|FD-Score:-3.26|P-value:5.60E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:POP3(YNL282W)|FD-Score:-4.24|P-value:1.11E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPN6(YDL097C)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SEC7(YDR170C)|FD-Score:3.26|P-value:5.59E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SUA5(YGL169W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.07||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TAF10(YDR167W)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.08||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF12(YDR145W)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.03||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFA1(YKL028W)|FD-Score:3.37|P-value:3.83E-4|Clearance:0.05||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF11(YMR260C)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.02||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:BRF1(YGR246C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.02||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:BRL1(YHR036W)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.04||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CSE4(YKL049C)|FD-Score:5.61|P-value:1.02E-8|Clearance:2.07||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:DHR2(YKL078W)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.04||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DIS3(YOL021C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ESP1(YGR098C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.01||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GPI16(YHR188C)|FD-Score:3.1|P-value:9.68E-4|Clearance:0.17||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:GPN2(YOR262W)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.01||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HRR25(YPL204W)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.02||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSP60(YLR259C)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.02||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:LSM5(YER146W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.02||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM6(YGL201C)|FD-Score:-3.26|P-value:5.60E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:POP3(YNL282W)|FD-Score:-4.24|P-value:1.11E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPN6(YDL097C)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SEC7(YDR170C)|FD-Score:3.26|P-value:5.59E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SUA5(YGL169W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.07||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TAF10(YDR167W)|FD-Score:3.23|P-value:6.12E-4|Clearance:0.08||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF12(YDR145W)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.03||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFA1(YKL028W)|FD-Score:3.37|P-value:3.83E-4|Clearance:0.05||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF11(YMR260C)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.02||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4012779
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ARG4(YHR018C)|FD-Score:-4.49|P-value:3.57E-6||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ATG13(YPR185W)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATG21(YPL100W)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BNA4(YBL098W)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CIT3(YPR001W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:COA2(YPL189C-A)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CSF1(YLR087C)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTR1(YPR124W)|FD-Score:7.13|P-value:5.13E-13||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EFT2(YDR385W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:ELG1(YOR144C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FMP21(YBR269C_p)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP25(YLR077W)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV6(YNL133C)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAL11(YOL051W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCG1(YER163C_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GTT2(YLL060C)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HMO1(YDR174W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:KEL2(YGR238C)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KES1(YPL145C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:KEX1(YGL203C)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:LST7(YGR057C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYS12(YIL094C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAC1(YMR021C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MSB1(YOR188W)|FD-Score:7.23|P-value:2.43E-13||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSB2(YGR014W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSC2(YDR205W)|FD-Score:-6.6|P-value:2.02E-11||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSM1(YGR171C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:NAT3(YPR131C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NCR1(YPL006W)|FD-Score:-3.95|P-value:3.98E-5||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NGG1(YDR176W)|FD-Score:4.88|P-value:5.43E-7||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PET54(YGR222W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PFK26(YIL107C)|FD-Score:5|P-value:2.90E-7||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PIG2(YIL045W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PKP1(YIL042C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p Gene:PMS1(YNL082W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUS9(YDL036C)|FD-Score:-3.8|P-value:7.34E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:RCF2(YNR018W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RDS1(YCR106W)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RGD2(YFL047W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RHO2(YNL090W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPL17B(YJL177W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL1A(YPL220W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPN4(YDL020C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16B(YDL083C)|FD-Score:-5.06|P-value:2.12E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS26B(YER131W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:SAM4(YPL273W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SDC1(YDR469W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SET4(YJL105W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SET5(YHR207C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SGO1(YOR073W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHU2(YDR078C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIN4(YNL236W)|FD-Score:5.35|P-value:4.42E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SNQ2(YDR011W)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOD1(YJR104C)|FD-Score:5.37|P-value:3.91E-8||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOH1(YGL127C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRY1(YKL218C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:THI6(YPL214C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TKL1(YPR074C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:YBL055C(YBL055C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGR125W(YGR125W_p)|FD-Score:5.2|P-value:9.84E-8||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR127W(YGR127W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR153W(YGR153W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function Gene:YHC3(YJL059W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIM1(YMR152W)|FD-Score:4.36|P-value:6.58E-6||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YLR041W(YLR041W_d)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative protein of unknown function Gene:YLR374C(YLR374C_d)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR316C-B(YMR316C-B_d)|FD-Score:5.78|P-value:3.71E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL235C(YNL235C_d)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function Gene:YNR066C(YNR066C_p)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative membrane-localized protein of unknown function Gene:YPL205C(YPL205C_d)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YRA2(YKL214C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:ZWF1(YNL241C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AAD3(YCR107W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ARG4(YHR018C)|FD-Score:-4.49|P-value:3.57E-6||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ATG13(YPR185W)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATG21(YPL100W)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BNA4(YBL098W)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CIT3(YPR001W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:COA2(YPL189C-A)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CSF1(YLR087C)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTR1(YPR124W)|FD-Score:7.13|P-value:5.13E-13||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EFT2(YDR385W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:ELG1(YOR144C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FMP21(YBR269C_p)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP25(YLR077W)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV6(YNL133C)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAL11(YOL051W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCG1(YER163C_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GTT2(YLL060C)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HMO1(YDR174W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:KEL2(YGR238C)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KES1(YPL145C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:KEX1(YGL203C)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:LST7(YGR057C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYS12(YIL094C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAC1(YMR021C)|FD-Score:3.81|P-value:7.02E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MSB1(YOR188W)|FD-Score:7.23|P-value:2.43E-13||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSB2(YGR014W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSC2(YDR205W)|FD-Score:-6.6|P-value:2.02E-11||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSM1(YGR171C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:NAT3(YPR131C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met Gene:NCR1(YPL006W)|FD-Score:-3.95|P-value:3.98E-5||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NGG1(YDR176W)|FD-Score:4.88|P-value:5.43E-7||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PET54(YGR222W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PFK26(YIL107C)|FD-Score:5|P-value:2.90E-7||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PIG2(YIL045W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PKP1(YIL042C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p Gene:PMS1(YNL082W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUS9(YDL036C)|FD-Score:-3.8|P-value:7.34E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:RCF2(YNR018W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RDS1(YCR106W)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RGD2(YFL047W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RHO2(YNL090W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPL17B(YJL177W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL1A(YPL220W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPN4(YDL020C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16B(YDL083C)|FD-Score:-5.06|P-value:2.12E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS26B(YER131W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:SAM4(YPL273W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SDC1(YDR469W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SET4(YJL105W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SET5(YHR207C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SGO1(YOR073W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHU2(YDR078C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIN4(YNL236W)|FD-Score:5.35|P-value:4.42E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SNQ2(YDR011W)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOD1(YJR104C)|FD-Score:5.37|P-value:3.91E-8||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOH1(YGL127C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRY1(YKL218C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:THI6(YPL214C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TKL1(YPR074C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:YBL055C(YBL055C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGR125W(YGR125W_p)|FD-Score:5.2|P-value:9.84E-8||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR127W(YGR127W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR153W(YGR153W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function Gene:YHC3(YJL059W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIM1(YMR152W)|FD-Score:4.36|P-value:6.58E-6||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YLR041W(YLR041W_d)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative protein of unknown function Gene:YLR374C(YLR374C_d)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR316C-B(YMR316C-B_d)|FD-Score:5.78|P-value:3.71E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL235C(YNL235C_d)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function Gene:YNR066C(YNR066C_p)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative membrane-localized protein of unknown function Gene:YPL205C(YPL205C_d)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YRA2(YKL214C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:ZWF1(YNL241C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL049C5.611.02E-82.07CSE4Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions
YGR246C3.542.02E-40.02BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YKL078W3.522.16E-40.04DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YGR098C3.482.48E-40.01ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YGL169W3.472.59E-40.07SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YLR259C3.403.33E-40.02HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YPL204W3.383.60E-40.02HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YKL028W3.373.83E-40.05TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YHR036W3.324.56E-40.04BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YOR262W3.275.32E-40.01GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YDR170C3.265.59E-40.00SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
YMR260C3.265.65E-40.02TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YDR167W3.236.12E-40.08TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YDR145W3.168.00E-40.03TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YER146W3.129.00E-40.02LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR188W7.232.43E-13MSB1Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
YPR124W7.135.13E-13CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YMR316C-B_d5.783.71E-9YMR316C-B_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJR104C5.373.91E-8SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YNL236W5.354.42E-8SIN4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
YGR125W_p5.209.84E-8YGR125W_pPutative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YIL107C5.002.90E-7PFK266-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A
YDR176W4.885.43E-7NGG1Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
YNL133C4.865.75E-7FYV6Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
YNR066C_p4.751.00E-6YNR066C_pPutative membrane-localized protein of unknown function
YBL098W4.651.70E-6BNA4Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease
YLR087C4.562.58E-6CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPL100W4.532.95E-6ATG21Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein
YCR106W4.523.15E-6RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YNL241C4.464.01E-6ZWF1Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_740
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1102.75E-17SGTC_7251497-0963 19.0 μMChemDiv (Drug-like library)35499770.3copper-dependent oxidative stress
0.1063.09E-16SGTC_8590439-0102 54.6 μMChemDiv (Drug-like library)6101430.102564redox potentiating
0.1022.91E-15SGTC_2248tofa 1.1 μMMiscellaneous1151750.0722892sphingolipid biosynthesis & PDR1
0.1024.37E-15SGTC_1000086-0128 49.6 μMChemDiv (Drug-like library)932970.2copper-dependent oxidative stress
0.1024.37E-15SGTC_2681menadione 3.2 μMMiscellaneous40550.084507superoxide
0.0921.51E-12SGTC_1563474-0145 5.6 μMChemDiv (Drug-like library)22654710.166667endomembrane recycling
0.0921.57E-12SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.0886076superoxide
0.0921.60E-12SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.0958904copper-dependent oxidative stress
0.0912.34E-12SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.0704225copper-dependent oxidative stress
0.0896.12E-12SGTC_1060092-0003 11.6 μMChemDiv (Drug-like library)10721480.16copper-dependent oxidative stress
0.0881.40E-11SGTC_6260214-0009 33.0 μMChemDiv (Drug-like library)34378740.28169copper-dependent oxidative stress
0.0864.67E-11SGTC_10004048-0884 8.7 μMChemDiv (Drug-like library)130400880.185185copper-dependent oxidative stress
0.0841.02E-10SGTC_27815567276 71.4 μMChembridge (Drug-like library)28532740.103448
0.0841.12E-10SGTC_25483',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin 19.9 μMMicrosource (Natural product library)40002430.0941176redox potentiating
0.0841.17E-10SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.0779221superoxide

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_11831254-0324198 μM0.5217395799357ChemDiv (Drug-like library)403.186941.49725
SGTC_1340269-00186.92 μM0.5081974378915ChemDiv (Drug-like library)411.121283.04617
SGTC_990109-0120622.8 μM0.42623759414ChemDiv (Drug-like library)321.126163.25713
SGTC_20545233173129 μM0.3333336889Chembridge (Fragment library)226.026861.41812mitochondrial response to ROS
SGTC_13031160-0052261 μM0.3055563562476ChemDiv (Drug-like library)281.266142.87413
SGTC_1240136-02177.12 μM0.3037973976905ChemDiv (Drug-like library)476.223245.16417mitochondrial stress
SGTC_196k015-002318.11 μM0.301375211998ChemDiv (Drug-like library)361.146963.10204PDR1
SGTC_7251497-096319 μM0.33549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress
SGTC_8680527-0199213 μM0.2894742849450ChemDiv (Drug-like library)365.138963.85727fatty acid desaturase (OLE1)
SGTC_6260214-000933 μM0.281693437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress