1594-0068

5-(4-chlorophenyl)-1,3,4-thiadiazol-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_744
Screen concentration 283.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 668392
SMILES C1=CC(=CC=C1C2=NN=C(S2)N)Cl
Standardized SMILES Nc1nnc(s1)c2ccc(Cl)cc2
Molecular weight 211.6713
ALogP 2.15
H-bond donor count 1
H-bond acceptor count 4
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.96
% growth inhibition (Hom. pool) 12.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 668392
Download HIP data (tab-delimited text)  (excel)
Gene:BRL1(YHR036W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.11||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:DIS3(YOL021C)|FD-Score:3.18|P-value:7.30E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:LAS1(YKR063C)|FD-Score:-3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:NUP49(YGL172W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.04||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUT2(YPR168W)|FD-Score:3.58|P-value:1.75E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:PAB1(YER165W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.48||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE4(YFR050C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.16||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPB8(YOR224C)|FD-Score:4.36|P-value:6.56E-6|Clearance:0.17||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:4.51|P-value:3.32E-6|Clearance:0.15||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.03||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.08||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPO31(YOR116C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.17||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:7.37|P-value:8.35E-14|Clearance:2.87||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SDH3(YKL141W)|FD-Score:-3.42|P-value:3.09E-4|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:TPT1(YOL102C)|FD-Score:-6|P-value:9.68E-10|Clearance:0||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide Gene:BRL1(YHR036W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.11||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:DIS3(YOL021C)|FD-Score:3.18|P-value:7.30E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:LAS1(YKR063C)|FD-Score:-3.82|P-value:6.63E-5|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:NUP49(YGL172W)|FD-Score:3.38|P-value:3.59E-4|Clearance:0.04||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUT2(YPR168W)|FD-Score:3.58|P-value:1.75E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:PAB1(YER165W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.48||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE4(YFR050C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.16||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPB8(YOR224C)|FD-Score:4.36|P-value:6.56E-6|Clearance:0.17||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:4.51|P-value:3.32E-6|Clearance:0.15||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.03||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.08||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPO31(YOR116C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.17||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:7.37|P-value:8.35E-14|Clearance:2.87||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SDH3(YKL141W)|FD-Score:-3.42|P-value:3.09E-4|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:TPT1(YOL102C)|FD-Score:-6|P-value:9.68E-10|Clearance:0||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 668392
Download HOP data (tab-delimited text)  (excel)
Gene:ABM1(YJR108W)|FD-Score:-5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACS1(YAL054C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ATP11(YNL315C)|FD-Score:5.85|P-value:2.49E-9||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP12(YJL180C)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP14(YLR295C)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP17(YDR377W)|FD-Score:4.79|P-value:8.35E-7||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP2(YJR121W)|FD-Score:5.27|P-value:6.69E-8||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD31(YCR063W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CBT1(YKL208W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CIN8(YEL061C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CIS3(YJL158C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:CLB2(YPR119W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG6(YNL041C)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX14(YML129C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CTF8(YHR191C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CYS4(YGR155W)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:10.2|P-value:7.21E-25||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ELG1(YOR144C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FPS1(YLL043W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FRE7(YOL152W)|FD-Score:-3.73|P-value:9.55E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GEP4(YHR100C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLC8(YMR311C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HMX1(YLR205C)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:HPR1(YDR138W)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HTA1(YDR225W)|FD-Score:7.55|P-value:2.24E-14||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IKI1(YHR187W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:LAS21(YJL062W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LCB5(YLR260W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LCL2(YLR104W_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:MRE11(YMR224C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS17(YMR188C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSW1(YDR268W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTF2(YDL044C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NBP2(YDR162C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PAD1(YDR538W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PDR11(YIL013C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:RNH70(YGR276C)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RNP1(YLL046C)|FD-Score:-5.41|P-value:3.15E-8||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPA34(YJL148W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPB9(YGL070C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL1A(YPL220W)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RSC1(YGR056W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTC3(YHR087W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SET4(YJL105W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIN4(YNL236W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKI3(YPR189W)|FD-Score:4.78|P-value:8.87E-7||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SSD1(YDR293C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STR2(YJR130C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:SUV3(YPL029W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWS2(YNL081C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAX4(YJL083W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRP3(YKL211C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS85(YDR108W)|FD-Score:9.6|P-value:3.95E-22||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TUB3(YML124C)|FD-Score:5.2|P-value:9.97E-8||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:URA7(YBL039C)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:YAL004W(YAL004W_d)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function Gene:YDR095C(YDR095C_d)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:3.86|P-value:5.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER134C(YER134C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YER156C(YER156C_p)|FD-Score:4.95|P-value:3.79E-7||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL149W(YGL149W_d)|FD-Score:-3.75|P-value:8.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YIL028W(YIL028W_d)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function Gene:YMR090W(YMR090W_p)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR175W-A(YMR175W-A_p)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Putative protein of unknown function Gene:YNL211C(YNL211C_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL099C(YOL099C_d)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YOL118C(YOL118C_d)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR345C(YOR345C_d)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPR099C(YPR099C_d)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YSP1(YHR155W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YTA12(YMR089C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:YTP1(YNL237W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZRT2(YLR130C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:ABM1(YJR108W)|FD-Score:-5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACS1(YAL054C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ATP11(YNL315C)|FD-Score:5.85|P-value:2.49E-9||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP12(YJL180C)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP14(YLR295C)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP17(YDR377W)|FD-Score:4.79|P-value:8.35E-7||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP2(YJR121W)|FD-Score:5.27|P-value:6.69E-8||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD31(YCR063W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CBT1(YKL208W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CIN8(YEL061C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CIS3(YJL158C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:CLB2(YPR119W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG6(YNL041C)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX14(YML129C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CTF8(YHR191C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CYS4(YGR155W)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:10.2|P-value:7.21E-25||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUN1(YDL101C)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ELG1(YOR144C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FPS1(YLL043W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FRE7(YOL152W)|FD-Score:-3.73|P-value:9.55E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GEP4(YHR100C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLC8(YMR311C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HMX1(YLR205C)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:HPR1(YDR138W)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HTA1(YDR225W)|FD-Score:7.55|P-value:2.24E-14||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IKI1(YHR187W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:LAS21(YJL062W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LCB5(YLR260W)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LCL2(YLR104W_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:MRE11(YMR224C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS17(YMR188C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSW1(YDR268W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTF2(YDL044C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NBP2(YDR162C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PAD1(YDR538W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PDR11(YIL013C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:RNH70(YGR276C)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RNP1(YLL046C)|FD-Score:-5.41|P-value:3.15E-8||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPA34(YJL148W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPB9(YGL070C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL1A(YPL220W)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RSC1(YGR056W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTC3(YHR087W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SET4(YJL105W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIN4(YNL236W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKI3(YPR189W)|FD-Score:4.78|P-value:8.87E-7||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SSD1(YDR293C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STR2(YJR130C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:SUV3(YPL029W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWS2(YNL081C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAX4(YJL083W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRP3(YKL211C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS85(YDR108W)|FD-Score:9.6|P-value:3.95E-22||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TUB3(YML124C)|FD-Score:5.2|P-value:9.97E-8||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:URA7(YBL039C)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:YAL004W(YAL004W_d)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function Gene:YDR095C(YDR095C_d)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR509W(YDR509W_d)|FD-Score:3.86|P-value:5.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER134C(YER134C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YER156C(YER156C_p)|FD-Score:4.95|P-value:3.79E-7||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL149W(YGL149W_d)|FD-Score:-3.75|P-value:8.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YIL028W(YIL028W_d)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Putative protein of unknown function Gene:YMR090W(YMR090W_p)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR175W-A(YMR175W-A_p)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Putative protein of unknown function Gene:YNL211C(YNL211C_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YOL099C(YOL099C_d)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YOL118C(YOL118C_d)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR345C(YOR345C_d)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPR099C(YPR099C_d)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YSP1(YHR155W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YTA12(YMR089C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:YTP1(YNL237W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZRT2(YLR130C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C7.378.35E-142.87RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YFR004W4.513.32E-60.15RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YOR224C4.366.56E-60.17RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YER165W4.191.40E-50.48PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YER021W3.711.02E-40.03RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YHR036W3.691.13E-40.11BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YPR168W3.581.75E-40.02NUT2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
YOR116C3.551.92E-40.17RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YGL172W3.383.59E-40.04NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YFR050C3.344.17E-40.16PRE4Beta 7 subunit of the 20S proteasome
YOL021C3.187.30E-40.03DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDL147W3.158.16E-40.08RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YMR208W3.070.001090.04ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR172W3.020.001260.03SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YDR449C2.990.001380.07UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W10.207.21E-25DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR108W9.603.95E-22TRS85Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
YDR225W7.552.24E-14HTA1Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
YNL315C5.852.49E-9ATP11Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase
YJL180C5.611.01E-8ATP12Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
YJR121W5.276.69E-8ATP2Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YML124C5.209.97E-8TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YBL039C5.121.52E-7URA7Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication
YNL041C5.052.25E-7COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YOL118C_d4.983.14E-7YOL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YER156C_p4.953.79E-7YER156C_pPutative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
YDR377W4.798.35E-7ATP17Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YPR189W4.788.87E-7SKI3Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YML021C4.661.61E-6UNG1Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
YMR175W-A_p4.621.88E-6YMR175W-A_pPutative protein of unknown function

GO enrichment analysis for SGTC_744
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1912.36E-49SGTC_24605552655 174.2 μMMiscellaneous54019890.176471amide catabolism
0.1823.74E-45SGTC_486niguldipine 82.0 μMMiscellaneous12360.0344828amide catabolism
0.1758.99E-42SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.05amide catabolism
0.1552.72E-33SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.0731707RNA pol III & RNase P/MRP
0.1482.97E-30SGTC_389tpck 16.7 μMMiscellaneous4396470.0677966
0.1483.48E-30SGTC_31679103034 49.5 μMChembridge (Drug-like library)51278590.0508475RPP1 & pyrimidine depletion
0.1461.25E-29SGTC_8800833-0487 87.2 μMChemDiv (Drug-like library)760750.25641mitochondrial processes
0.1403.57E-27SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.156863amide catabolism
0.1372.96E-26SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.233333amide catabolism
0.1374.39E-26SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.114754amide catabolism
0.1361.32E-25SGTC_2680butyl paraben 22.2 μMMicrosource (Natural product library)71840.0612245amide catabolism
0.1352.92E-25SGTC_1853st056710 91.2 μMTimTec (Natural product derivative library)2691670.0612245amide catabolism
0.1287.69E-23SGTC_23709071495 200.0 μMChembridge (Fragment library)217911030.116279mitochondrial processes
0.1279.93E-23SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.0535714RNA pol III & RNase P/MRP
0.1262.34E-22SGTC_1751st044839 27.2 μMTimTec (Natural product derivative library)52913260.0447761Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21275253358187.91 μM0.4062520612Chembridge (Fragment library)221.646321.77825
SGTC_12380333-028748.8 μM0.3611117226ChemDiv (Drug-like library)184.6462.37313Golgi
SGTC_9871497-1013404 μM0.2884621676435ChemDiv (Drug-like library)362.87526.58603
SGTC_9332914-0560114 μM0.285714463887ChemDiv (Drug-like library)234.704682.91302
SGTC_10124112-352218 μM0.2857143112398ChemDiv (Drug-like library)322.836243.81326
SGTC_13131220-0047237 μM0.2857143528706ChemDiv (Drug-like library)231.67763.15212
SGTC_660109-018742.4 μM0.275759417ChemDiv (Drug-like library)228.676963.93511
SGTC_8800833-048787.2 μM0.2564176075ChemDiv (Drug-like library)252.334183.54513mitochondrial processes
SGTC_270485-000132.62 μM0.2558142054479ChemDiv (Drug-like library)266.360764.32313RPP1 & pyrimidine depletion
SGTC_222nsc-31034210.67 μM0.25432556Miscellaneous268.722662.25525