0159-0059

ethyl (2Z)-2-[(4-nitrophenyl)hydrazinylidene]propanoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_75
Screen concentration 199.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5377270
SMILES CCOC(=O)C(=NNC1=CC=C(C=C1)[N+](=O)[O-])C
Standardized SMILES CCOC(=O)C(=NNc1ccc(cc1)[N+](=O)[O-])C
Molecular weight 251.2386
ALogP 2
H-bond donor count 1
H-bond acceptor count 6
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.58
% growth inhibition (Hom. pool) 5.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5377270
Download HIP data (tab-delimited text)  (excel)
Gene:APC2(YLR127C)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.04||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CAK1(YFL029C)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.06||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CBK1(YNL161W)|FD-Score:-3.09|P-value:9.97E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC9(YDL164C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:DIS3(YOL021C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.01||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DPS1(YLL018C)|FD-Score:-3.81|P-value:7.03E-5|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ENP1(YBR247C)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.04||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:GDI1(YER136W)|FD-Score:3.86|P-value:5.63E-5|Clearance:0.22||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPI13(YLL031C)|FD-Score:-5.11|P-value:1.58E-7|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:HEM4(YOR278W)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.17||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MCM10(YIL150C)|FD-Score:-3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MPP10(YJR002W)|FD-Score:3.22|P-value:6.30E-4|Clearance:0.1||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:MPS2(YGL075C)|FD-Score:-5.35|P-value:4.51E-8|Clearance:0||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NDC1(YML031W)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NSA2(YER126C)|FD-Score:4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:ORC4(YPR162C)|FD-Score:5.27|P-value:6.88E-8|Clearance:0.77||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:ORC6(YHR118C)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.1||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RPP1(YHR062C)|FD-Score:-4.24|P-value:1.12E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.04||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP36(YOR287C)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.17||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:RRP45(YDR280W)|FD-Score:5.53|P-value:1.64E-8|Clearance:0.77||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP9(YPR137W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.01||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RVB1(YDR190C)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.07||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:4.5|P-value:3.46E-6|Clearance:0.26||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SPC3(YLR066W)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.11||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SPC34(YKR037C)|FD-Score:-3.87|P-value:5.44E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SRP1(YNL189W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TFB1(YDR311W)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:YDR053W(YDR053W_d)|FD-Score:6.29|P-value:1.55E-10|Clearance:0.77||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YKL111C(YKL111C_d)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.07||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:APC2(YLR127C)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.04||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CAK1(YFL029C)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.06||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CBK1(YNL161W)|FD-Score:-3.09|P-value:9.97E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC9(YDL164C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:DIS3(YOL021C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.01||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DPS1(YLL018C)|FD-Score:-3.81|P-value:7.03E-5|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ENP1(YBR247C)|FD-Score:3.94|P-value:4.11E-5|Clearance:0.04||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:GDI1(YER136W)|FD-Score:3.86|P-value:5.63E-5|Clearance:0.22||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPI13(YLL031C)|FD-Score:-5.11|P-value:1.58E-7|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:HEM4(YOR278W)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.17||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MCM10(YIL150C)|FD-Score:-3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MPP10(YJR002W)|FD-Score:3.22|P-value:6.30E-4|Clearance:0.1||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:MPS2(YGL075C)|FD-Score:-5.35|P-value:4.51E-8|Clearance:0||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NDC1(YML031W)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NSA2(YER126C)|FD-Score:4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:ORC4(YPR162C)|FD-Score:5.27|P-value:6.88E-8|Clearance:0.77||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:ORC6(YHR118C)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.1||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RPP1(YHR062C)|FD-Score:-4.24|P-value:1.12E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.04||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP36(YOR287C)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.17||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:RRP45(YDR280W)|FD-Score:5.53|P-value:1.64E-8|Clearance:0.77||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP9(YPR137W)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.01||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RVB1(YDR190C)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.07||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:4.5|P-value:3.46E-6|Clearance:0.26||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SPC3(YLR066W)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.11||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SPC34(YKR037C)|FD-Score:-3.87|P-value:5.44E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SRP1(YNL189W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TFB1(YDR311W)|FD-Score:4.23|P-value:1.16E-5|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:YDR053W(YDR053W_d)|FD-Score:6.29|P-value:1.55E-10|Clearance:0.77||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YKL111C(YKL111C_d)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.07||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5377270
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:11.5|P-value:3.87E-31||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ASI1(YMR119W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG36(YJL185C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:AVT7(YIL088C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI4(YNL233W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCC2(YDR270W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CCP1(YKR066C)|FD-Score:-5.81|P-value:3.16E-9||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CIN2(YPL241C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:COA6(YMR244C-A_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:DCW1(YKL046C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:FEN2(YCR028C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FLC2(YAL053W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GCS1(YDL226C)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDH2(YDL215C)|FD-Score:5.35|P-value:4.48E-8||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GPM3(YOL056W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HAP5(YOR358W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:ISC1(YER019W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:IST3(YIR005W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:JSN1(YJR091C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:NBA1(YOL070C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:OCA1(YNL099C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PEX3(YDR329C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PKH2(YOL100W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PLB3(YOL011W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PRS5(YOL061W)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PTC3(YBL056W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RBL2(YOR265W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RCY1(YJL204C)|FD-Score:8.38|P-value:2.60E-17||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RME1(YGR044C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL23B(YER117W)|FD-Score:6.09|P-value:5.69E-10||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:SCH9(YHR205W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SGE1(YPR198W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHE2(YKL130C)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SKG6(YHR149C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SNA3(YJL151C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF12(YNR023W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNZ2(YNL333W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPS2(YDR522C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SSH4(YKL124W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SUR4(YLR372W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TMA108(YIL137C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:UTP30(YKR060W)|FD-Score:4.39|P-value:5.61E-6||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VAB2(YEL005C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VID22(YLR373C)|FD-Score:7.41|P-value:6.13E-14||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA1(YDL185W)|FD-Score:5.08|P-value:1.85E-7||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:5.7|P-value:6.06E-9||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS65(YLR322W_d)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YCR006C(YCR006C_d)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR064C(YCR064C_d)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR018C(YDR018C_p)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR336W(YDR336W_p)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YDR379C-A(YDR379C-A)|FD-Score:-3.86|P-value:5.63E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YER119C-A(YER119C-A_d)|FD-Score:5.62|P-value:9.55E-9||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFR056C(YFR056C_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Putative protein of unknown function Gene:YGR228W(YGR228W_d)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR112C(YHR112C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YJL055W(YJL055W_p)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL033W-A(YKL033W-A_p)|FD-Score:5.25|P-value:7.78E-8||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR104W(YKR104W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YMR310C(YMR310C_p)|FD-Score:5.83|P-value:2.83E-9||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.92|P-value:4.24E-7||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL077C(YPL077C_p)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL119C-A(YPL119C-A_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR150W(YPR150W_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:ACB1(YGR037C)|FD-Score:11.5|P-value:3.87E-31||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ASI1(YMR119W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG36(YJL185C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:AVT7(YIL088C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BNI4(YNL233W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCC2(YDR270W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CCP1(YKR066C)|FD-Score:-5.81|P-value:3.16E-9||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CIN2(YPL241C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:COA6(YMR244C-A_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:DCW1(YKL046C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:FEN2(YCR028C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FLC2(YAL053W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GCS1(YDL226C)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDH2(YDL215C)|FD-Score:5.35|P-value:4.48E-8||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GPM3(YOL056W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HAP5(YOR358W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:ISC1(YER019W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:IST3(YIR005W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:JSN1(YJR091C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:NBA1(YOL070C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:OCA1(YNL099C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PEX3(YDR329C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PKH2(YOL100W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PLB3(YOL011W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PRS5(YOL061W)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PTC3(YBL056W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RBL2(YOR265W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RCY1(YJL204C)|FD-Score:8.38|P-value:2.60E-17||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RME1(YGR044C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL23B(YER117W)|FD-Score:6.09|P-value:5.69E-10||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:SCH9(YHR205W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SGE1(YPR198W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHE2(YKL130C)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SKG6(YHR149C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SNA3(YJL151C)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles Gene:SNF12(YNR023W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNZ2(YNL333W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPS2(YDR522C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SSH4(YKL124W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SUR4(YLR372W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TMA108(YIL137C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:UTP30(YKR060W)|FD-Score:4.39|P-value:5.61E-6||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VAB2(YEL005C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VID22(YLR373C)|FD-Score:7.41|P-value:6.13E-14||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA1(YDL185W)|FD-Score:5.08|P-value:1.85E-7||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:5.7|P-value:6.06E-9||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS65(YLR322W_d)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YCR006C(YCR006C_d)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR064C(YCR064C_d)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR018C(YDR018C_p)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR336W(YDR336W_p)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YDR379C-A(YDR379C-A)|FD-Score:-3.86|P-value:5.63E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YER119C-A(YER119C-A_d)|FD-Score:5.62|P-value:9.55E-9||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFR056C(YFR056C_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Putative protein of unknown function Gene:YGR228W(YGR228W_d)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR112C(YHR112C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YJL055W(YJL055W_p)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL033W-A(YKL033W-A_p)|FD-Score:5.25|P-value:7.78E-8||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR104W(YKR104W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YMR310C(YMR310C_p)|FD-Score:5.83|P-value:2.83E-9||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.92|P-value:4.24E-7||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL077C(YPL077C_p)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL119C-A(YPL119C-A_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR150W(YPR150W_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR053W_d6.291.55E-100.77YDR053W_dPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
YDR280W5.531.64E-80.77RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YPR162C5.276.88E-80.77ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YGR195W4.503.46E-60.27SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YDR311W4.231.16E-50.01TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YER126C4.221.21E-50.00NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YLR127C4.221.23E-50.04APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
YOR287C4.181.45E-50.17RRP36Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
YKL111C_d4.013.02E-50.07YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YBR247C3.944.11E-50.04ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YLR167W3.904.77E-50.04RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YER136W3.865.63E-50.22GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YDL164C3.641.35E-40.00CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YDR190C3.641.36E-40.07RVB1ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YFL029C3.571.76E-40.06CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR037C11.503.87E-31ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YJL204C8.382.60E-17RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YLR373C7.416.13E-14VID22Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles
YER117W6.095.69E-10RPL23BRibosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
YMR310C_p5.832.83E-9YMR310C_pPutative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene
YPR036W5.706.06E-9VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YER119C-A_d5.629.55E-9YER119C-A_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
YNL333W5.601.07E-8SNZ2Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p
YDL215C5.354.48E-8GDH2NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels
YKL033W-A_p5.257.78E-8YKL033W-A_pPutative protein of unknown function; similar to uncharacterized proteins from other fungi
YDL185W5.081.85E-7VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YOR034C-A_p4.924.24E-7YOR034C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YDL226C4.661.55E-6GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YJL055W_p4.542.85E-6YJL055W_pPutative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU
YKR060W4.395.61E-6UTP30Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit

GO enrichment analysis for SGTC_75
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1256.95E-22SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.0869565fatty acid desaturase (OLE1)
0.1224.80E-21SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.351852fatty acid desaturase (OLE1)
0.1201.57E-20SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.316667fatty acid desaturase (OLE1)
0.1156.38E-19SGTC_258haloperidol 50.8 μMMiscellaneous35590.0945946fatty acid desaturase (OLE1)
0.1141.29E-18SGTC_2416tofa 880.0 nMMiscellaneous1151750.166667sphingolipid biosynthesis & PDR1
0.1133.39E-18SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.0533333endomembrane recycling
0.1128.09E-18SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.0833333fatty acid desaturase (OLE1)
0.1063.76E-16SGTC_218alverine citrate 93.8 μMMiscellaneous217180.111111fatty acid desaturase (OLE1)
0.1054.38E-16SGTC_1660st012960 28.1 μMTimTec (Natural product derivative library)242072660.144928iron homeostasis
0.1054.84E-16SGTC_2725haloperidol 53.2 μMMiscellaneous35590.0945946plasma membrane duress
0.1056.67E-16SGTC_6880527-0193 473.0 μMChemDiv (Drug-like library)40071540.327586fatty acid desaturase (OLE1)
0.1031.77E-15SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.126761fatty acid desaturase (OLE1)
0.1001.72E-14SGTC_13943474-0001 65.7 μMChemDiv (Drug-like library)50176660.0857143endomembrane recycling
0.0991.90E-14SGTC_6104215-0184 155.0 μMChemDiv (Drug-like library)56050150.1exosome
0.0992.22E-14SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.0909091fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1030844-010451.84 μM0.6585375375804ChemDiv (Drug-like library)206.241062.10514ERAD & cell cycle
SGTC_3730844-010425.9 μM0.6585375375804ChemDiv (Drug-like library)206.241062.10514ERAD & cell cycle
SGTC_6371181-05197.26 μM0.5652176252091ChemDiv (Drug-like library)250.253860.70925fatty acid desaturase (OLE1)
SGTC_8340312-000875.7 μM0.46385573ChemDiv (Drug-like library)210.660142.29613NEO1
SGTC_850868-0259160.06 μM0.3883173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_13281441-002569.2 μM0.370374562601ChemDiv (Drug-like library)287.270722.2724
SGTC_990109-0120622.8 μM0.36759414ChemDiv (Drug-like library)321.126163.25713
SGTC_5681193-00977.48 μM0.363646150ChemDiv (Drug-like library)242.658941.98724ERAD & cell cycle
SGTC_8750709-021025.7 μM0.3518526251364ChemDiv (Drug-like library)281.266143.07815fatty acid desaturase (OLE1)
SGTC_22727805186180 μM0.3518522956062Chembridge (Fragment library)268.335342.54524cell wall signaling
SGTC_6880527-0193473 μM0.3275864007154ChemDiv (Drug-like library)286.24293.10827fatty acid desaturase (OLE1)