4073-0104

1-(2-chlorophenyl)-5-(3-methoxy-4-phenylmethoxyphenyl)-1,2,4-triazole

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_750
Screen concentration 132.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 22330957
SMILES COC1=C(C=CC(=C1)C2=NC=NN2C3=CC=CC=C3Cl)OCC4=CC=CC=C4
Standardized SMILES COc1cc(ccc1OCc2ccccc2)c3ncnn3c4ccccc4Cl
Molecular weight 391.8502
ALogP 5.51
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.22
% growth inhibition (Hom. pool) 8.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 22330957
Download HIP data (tab-delimited text)  (excel)
Gene:CDC16(YKL022C)|FD-Score:-5.03|P-value:2.52E-7|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC23(YHR166C)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COF1(YLL050C)|FD-Score:-5.45|P-value:2.52E-8|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CWC24(YLR323C)|FD-Score:-3.51|P-value:2.20E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:ECO1(YFR027W)|FD-Score:-4.3|P-value:8.46E-6|Clearance:0||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:FAF1(YIL019W)|FD-Score:-5.91|P-value:1.73E-9|Clearance:0||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:GPI1(YGR216C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:GRC3(YLL035W)|FD-Score:-3.94|P-value:4.01E-5|Clearance:0||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:HEM13(YDR044W)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HSP60(YLR259C)|FD-Score:-4.65|P-value:1.70E-6|Clearance:0||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:IFH1(YLR223C)|FD-Score:-5.44|P-value:2.64E-8|Clearance:0||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:KRS1(YDR037W)|FD-Score:-4.95|P-value:3.73E-7|Clearance:0||SGD DESC:Lysyl-tRNA synthetase Gene:LTO1(YNL260C_p)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:NCP1(YHR042W)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NSL1(YPL233W)|FD-Score:-4.41|P-value:5.25E-6|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUT2(YPR168W)|FD-Score:-3.76|P-value:8.54E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:OKP1(YGR179C)|FD-Score:-3.2|P-value:6.96E-4|Clearance:0||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:OST1(YJL002C)|FD-Score:-3.78|P-value:7.69E-5|Clearance:0||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PDC2(YDR081C)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.1||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRO3(YER023W)|FD-Score:-4.96|P-value:3.54E-7|Clearance:0||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:PSE1(YMR308C)|FD-Score:-4.54|P-value:2.81E-6|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:RAD3(YER171W)|FD-Score:-4.69|P-value:1.40E-6|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RFA2(YNL312W)|FD-Score:-5.33|P-value:4.92E-8|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPA135(YPR010C)|FD-Score:-4.6|P-value:2.14E-6|Clearance:0||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RSA4(YCR072C)|FD-Score:-5.27|P-value:6.82E-8|Clearance:0||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:RSP5(YER125W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SDH3(YKL141W)|FD-Score:-5.38|P-value:3.66E-8|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SRP21(YKL122C)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:STT4(YLR305C)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:SWP1(YMR149W)|FD-Score:-3.51|P-value:2.20E-4|Clearance:0||SGD DESC:Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:TAO3(YIL129C)|FD-Score:-3.95|P-value:3.89E-5|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TIM12(YBR091C)|FD-Score:-3.96|P-value:3.77E-5|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TOM20(YGR082W)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins Gene:YGL069C(YGL069C_d)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.25||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:CDC16(YKL022C)|FD-Score:-5.03|P-value:2.52E-7|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC23(YHR166C)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COF1(YLL050C)|FD-Score:-5.45|P-value:2.52E-8|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CWC24(YLR323C)|FD-Score:-3.51|P-value:2.20E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:ECO1(YFR027W)|FD-Score:-4.3|P-value:8.46E-6|Clearance:0||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:FAF1(YIL019W)|FD-Score:-5.91|P-value:1.73E-9|Clearance:0||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:GPI1(YGR216C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:GRC3(YLL035W)|FD-Score:-3.94|P-value:4.01E-5|Clearance:0||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:HEM13(YDR044W)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HSP60(YLR259C)|FD-Score:-4.65|P-value:1.70E-6|Clearance:0||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:IFH1(YLR223C)|FD-Score:-5.44|P-value:2.64E-8|Clearance:0||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:KRS1(YDR037W)|FD-Score:-4.95|P-value:3.73E-7|Clearance:0||SGD DESC:Lysyl-tRNA synthetase Gene:LTO1(YNL260C_p)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:NCP1(YHR042W)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NSL1(YPL233W)|FD-Score:-4.41|P-value:5.25E-6|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUT2(YPR168W)|FD-Score:-3.76|P-value:8.54E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:OKP1(YGR179C)|FD-Score:-3.2|P-value:6.96E-4|Clearance:0||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:OST1(YJL002C)|FD-Score:-3.78|P-value:7.69E-5|Clearance:0||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PDC2(YDR081C)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.1||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRO3(YER023W)|FD-Score:-4.96|P-value:3.54E-7|Clearance:0||SGD DESC:Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis Gene:PSE1(YMR308C)|FD-Score:-4.54|P-value:2.81E-6|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:RAD3(YER171W)|FD-Score:-4.69|P-value:1.40E-6|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RFA2(YNL312W)|FD-Score:-5.33|P-value:4.92E-8|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPA135(YPR010C)|FD-Score:-4.6|P-value:2.14E-6|Clearance:0||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RSA4(YCR072C)|FD-Score:-5.27|P-value:6.82E-8|Clearance:0||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:RSP5(YER125W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SDH3(YKL141W)|FD-Score:-5.38|P-value:3.66E-8|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SRP21(YKL122C)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:STT4(YLR305C)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:SWP1(YMR149W)|FD-Score:-3.51|P-value:2.20E-4|Clearance:0||SGD DESC:Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:TAO3(YIL129C)|FD-Score:-3.95|P-value:3.89E-5|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TIM12(YBR091C)|FD-Score:-3.96|P-value:3.77E-5|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TOM20(YGR082W)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins Gene:YGL069C(YGL069C_d)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.25||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 22330957
Download HOP data (tab-delimited text)  (excel)
Gene:ABM1(YJR108W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACH1(YBL015W)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ACS1(YAL054C)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ALB1(YJL122W)|FD-Score:-3.9|P-value:4.90E-5||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARG5,6(YER069W)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARN1(YHL040C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:ATP10(YLR393W)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BAP3(YDR046C)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BDH1(YAL060W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BDH2(YAL061W_p)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BIO4(YNR057C)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BOP2(YLR267W_p)|FD-Score:-6.26|P-value:1.97E-10||SGD DESC:Protein of unknown function Gene:BUD8(YLR353W)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole Gene:CIN8(YEL061C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COA2(YPL189C-A)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COQ10(YOL008W)|FD-Score:-3.82|P-value:6.65E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COY1(YKL179C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CSM3(YMR048W)|FD-Score:-6.75|P-value:7.21E-12||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:CTK1(YKL139W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DBP1(YPL119C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DBP3(YGL078C)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DGR2(YKL121W_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DJP1(YIR004W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DMC1(YER179W)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DUS3(YLR401C)|FD-Score:-5.36|P-value:4.19E-8||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:EAF3(YPR023C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:EMP24(YGL200C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:EMP70(YLR083C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ENT4(YLL038C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome Gene:FAT3(YKL187C_p)|FD-Score:-3.74|P-value:9.23E-5||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FCY2(YER056C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FCY22(YER060W-A)|FD-Score:-4.46|P-value:4.15E-6||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FIT1(YDR534C)|FD-Score:-4.35|P-value:6.68E-6||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP25(YLR077W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FOB1(YDR110W)|FD-Score:-4.82|P-value:7.18E-7||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FRE2(YKL220C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:FRE6(YLL051C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GDH3(YAL062W)|FD-Score:-3.68|P-value:1.14E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GEM1(YAL048C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GLT1(YDL171C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GSC2(YGR032W)|FD-Score:-5.78|P-value:3.78E-9||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GTT2(YLL060C)|FD-Score:-5.7|P-value:6.17E-9||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GYP8(YFL027C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:GZF3(YJL110C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HFM1(YGL251C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HMX1(YLR205C)|FD-Score:-7.5|P-value:3.24E-14||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:HPT1(YDR399W)|FD-Score:-4.96|P-value:3.59E-7||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:ICL1(YER065C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:IZH3(YLR023C)|FD-Score:-5.15|P-value:1.32E-7||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:KHA1(YJL094C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:LOT6(YLR011W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:LSB3(YFR024C-A)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MAD3(YJL013C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MAF1(YDR005C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MAP1(YLR244C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MBF1(YOR298C-A)|FD-Score:-3.86|P-value:5.76E-5||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MCK1(YNL307C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDS3(YGL197W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MEC3(YLR288C)|FD-Score:-6.03|P-value:8.18E-10||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MET14(YKL001C)|FD-Score:-3.73|P-value:9.67E-5||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MIH1(YMR036C)|FD-Score:-4.95|P-value:3.70E-7||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MRS1(YIR021W)|FD-Score:-5.31|P-value:5.42E-8||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSB1(YOR188W)|FD-Score:4.4|P-value:5.46E-6||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSS51(YLR203C)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NDE1(YMR145C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NRM1(YNR009W)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NST1(YNL091W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NUP188(YML103C)|FD-Score:-4.72|P-value:1.15E-6||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:PAU2(YEL049W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PBA1(YLR199C)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:PCL10(YGL134W)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PDX1(YGR193C)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PEX4(YGR133W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PHM7(YOL084W)|FD-Score:-3.72|P-value:9.99E-5||SGD DESC:Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress Gene:PIC2(YER053C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PIR1(YKL164C)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PMA2(YPL036W)|FD-Score:-4.99|P-value:2.96E-7||SGD DESC:Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential Gene:PML1(YLR016C)|FD-Score:-4.05|P-value:2.52E-5||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:POR2(YIL114C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:POT1(YIL160C)|FD-Score:-5.28|P-value:6.40E-8||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPM2(YOL141W)|FD-Score:-6.01|P-value:9.22E-10||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PRR1(YKL116C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:QRI7(YDL104C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RAD18(YCR066W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-3.78|P-value:7.80E-5||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RGT2(YDL138W)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication Gene:RIM11(YMR139W)|FD-Score:-4.64|P-value:1.70E-6||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RMA1(YKL132C)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RNP1(YLL046C)|FD-Score:-8.85|P-value:4.41E-19||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL37A(YLR185W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RSF2(YJR127C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHH3(YMR118C_p)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLT2(YHR030C)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT2(YER161C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:STB4(YMR019W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STB6(YKL072W)|FD-Score:-5.38|P-value:3.71E-8||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:STP2(YHR006W)|FD-Score:-4.5|P-value:3.39E-6||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SUR7(YML052W)|FD-Score:-8.02|P-value:5.36E-16||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:SYN8(YAL014C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TAD1(YGL243W)|FD-Score:-6.6|P-value:1.99E-11||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:TAX4(YJL083W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TDA2(YER071C_p)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TEF2(YBR118W)|FD-Score:-6.92|P-value:2.23E-12||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TGL1(YKL140W)|FD-Score:-5.57|P-value:1.25E-8||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:TIR1(YER011W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TIR2(YOR010C)|FD-Score:-5.72|P-value:5.27E-9||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:TKL1(YPR074C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA17(YDL110C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TPA1(YER049W)|FD-Score:-3.84|P-value:6.17E-5||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TRP3(YKL211C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSA1(YML028W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:UBP6(YFR010W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VHR2(YER064C_p)|FD-Score:-4.84|P-value:6.63E-7||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VPS21(YOR089C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS5(YOR069W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:WHI4(YDL224C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:WHI5(YOR083W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 Gene:YBL036C(YBL036C)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL011C(YDL011C_d)|FD-Score:-3.75|P-value:8.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR491C(YDR491C_d)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEA4(YEL004W)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER Gene:YEH1(YLL012W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:YER067C-A(YER067C-A_d)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YFL063W(YFL063W_d)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YGL081W(YGL081W_p)|FD-Score:-5.22|P-value:8.84E-8||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGL149W(YGL149W_d)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGL218W(YGL218W_d)|FD-Score:4.36|P-value:6.65E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR127W(YGR127W_p)|FD-Score:-5.98|P-value:1.13E-9||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR149W(YGR149W_p)|FD-Score:-3.75|P-value:8.78E-5||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YGR228W(YGR228W_d)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR003C(YHR003C)|FD-Score:-6.37|P-value:9.26E-11||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YJL068C(YJL068C)|FD-Score:-3.78|P-value:7.94E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL169W(YJL169W_d)|FD-Score:-6.44|P-value:5.81E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR056C(YJR056C_p)|FD-Score:-6.97|P-value:1.53E-12||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YJR142W(YJR142W_p)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL131W(YKL131W_d)|FD-Score:-3.87|P-value:5.50E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL151C(YKL151C)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YKR023W(YKR023W_p)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL020C(YLL020C_d)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR041W(YLR041W_d)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YLR283W(YLR283W_p)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-4.4|P-value:5.36E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YLR434C(YLR434C_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML053C(YML053C_p)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene Gene:YML083C(YML083C_p)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YML116W-A(YML116W-A_d)|FD-Score:-5.2|P-value:1.01E-7||SGD DESC:Putative protein of unknown function Gene:YMR007W(YMR007W_d)|FD-Score:-7.55|P-value:2.13E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR082C(YMR082C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR090W(YMR090W_p)|FD-Score:6.68|P-value:1.21E-11||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR099C(YMR099C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR111C(YMR111C_p)|FD-Score:-4.86|P-value:5.90E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR147W(YMR147W_p)|FD-Score:-5.78|P-value:3.74E-9||SGD DESC:Putative protein of unknown function Gene:YMR155W(YMR155W_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR182W-A(YMR182W-A_p)|FD-Score:-5.24|P-value:7.98E-8||SGD DESC:Putative protein of unknown function Gene:YMR210W(YMR210W)|FD-Score:-5.53|P-value:1.62E-8||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YNL043C(YNL043C_d)|FD-Score:4.44|P-value:4.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL303W(YNL303W_d)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Putative protein of unknown function Gene:YOR022C(YOR022C_p)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR062C(YOR062C_p)|FD-Score:-3.84|P-value:6.06E-5||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR300W(YOR300W_d)|FD-Score:-3.9|P-value:4.71E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOR345C(YOR345C_d)|FD-Score:-7.63|P-value:1.14E-14||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL107W(YPL107W_p)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL245W(YPL245W_p)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YRA2(YKL214C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YVC1(YOR087W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:ABM1(YJR108W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACH1(YBL015W)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ACS1(YAL054C)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ALB1(YJL122W)|FD-Score:-3.9|P-value:4.90E-5||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARG5,6(YER069W)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARN1(YHL040C)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:ATP10(YLR393W)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BAP3(YDR046C)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BDH1(YAL060W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BDH2(YAL061W_p)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BIO4(YNR057C)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BOP2(YLR267W_p)|FD-Score:-6.26|P-value:1.97E-10||SGD DESC:Protein of unknown function Gene:BUD8(YLR353W)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole Gene:CIN8(YEL061C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COA2(YPL189C-A)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COQ10(YOL008W)|FD-Score:-3.82|P-value:6.65E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COY1(YKL179C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CSM3(YMR048W)|FD-Score:-6.75|P-value:7.21E-12||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:CTK1(YKL139W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DBP1(YPL119C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DBP3(YGL078C)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DGR2(YKL121W_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DJP1(YIR004W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DMC1(YER179W)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DUS3(YLR401C)|FD-Score:-5.36|P-value:4.19E-8||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:EAF3(YPR023C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:EMP24(YGL200C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:EMP70(YLR083C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ENT4(YLL038C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome Gene:FAT3(YKL187C_p)|FD-Score:-3.74|P-value:9.23E-5||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FCY2(YER056C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FCY22(YER060W-A)|FD-Score:-4.46|P-value:4.15E-6||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FIT1(YDR534C)|FD-Score:-4.35|P-value:6.68E-6||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP25(YLR077W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FOB1(YDR110W)|FD-Score:-4.82|P-value:7.18E-7||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FRE2(YKL220C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:FRE6(YLL051C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GDH3(YAL062W)|FD-Score:-3.68|P-value:1.14E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GEM1(YAL048C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GLT1(YDL171C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GSC2(YGR032W)|FD-Score:-5.78|P-value:3.78E-9||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GTT2(YLL060C)|FD-Score:-5.7|P-value:6.17E-9||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:GYP8(YFL027C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:GZF3(YJL110C)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HFM1(YGL251C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HMX1(YLR205C)|FD-Score:-7.5|P-value:3.24E-14||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:HPT1(YDR399W)|FD-Score:-4.96|P-value:3.59E-7||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:ICL1(YER065C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:IZH3(YLR023C)|FD-Score:-5.15|P-value:1.32E-7||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:KHA1(YJL094C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:LOT6(YLR011W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:LSB3(YFR024C-A)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MAD3(YJL013C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MAF1(YDR005C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MAP1(YLR244C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MBF1(YOR298C-A)|FD-Score:-3.86|P-value:5.76E-5||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MCK1(YNL307C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDS3(YGL197W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MEC3(YLR288C)|FD-Score:-6.03|P-value:8.18E-10||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MET14(YKL001C)|FD-Score:-3.73|P-value:9.67E-5||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MIH1(YMR036C)|FD-Score:-4.95|P-value:3.70E-7||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MRS1(YIR021W)|FD-Score:-5.31|P-value:5.42E-8||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSB1(YOR188W)|FD-Score:4.4|P-value:5.46E-6||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSS51(YLR203C)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NDE1(YMR145C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NRM1(YNR009W)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NST1(YNL091W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NUP188(YML103C)|FD-Score:-4.72|P-value:1.15E-6||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:PAU2(YEL049W)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PBA1(YLR199C)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:PCL10(YGL134W)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PDX1(YGR193C)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PEX4(YGR133W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PHM7(YOL084W)|FD-Score:-3.72|P-value:9.99E-5||SGD DESC:Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress Gene:PIC2(YER053C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PIR1(YKL164C)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PMA2(YPL036W)|FD-Score:-4.99|P-value:2.96E-7||SGD DESC:Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential Gene:PML1(YLR016C)|FD-Score:-4.05|P-value:2.52E-5||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:POR2(YIL114C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:POT1(YIL160C)|FD-Score:-5.28|P-value:6.40E-8||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPM2(YOL141W)|FD-Score:-6.01|P-value:9.22E-10||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PRR1(YKL116C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:QRI7(YDL104C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RAD18(YCR066W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-3.78|P-value:7.80E-5||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RGT2(YDL138W)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication Gene:RIM11(YMR139W)|FD-Score:-4.64|P-value:1.70E-6||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RMA1(YKL132C)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RNP1(YLL046C)|FD-Score:-8.85|P-value:4.41E-19||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL37A(YLR185W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS1A(YLR441C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RSF2(YJR127C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHH3(YMR118C_p)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLT2(YHR030C)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT2(YER161C)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:STB4(YMR019W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STB6(YKL072W)|FD-Score:-5.38|P-value:3.71E-8||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:STP2(YHR006W)|FD-Score:-4.5|P-value:3.39E-6||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SUR7(YML052W)|FD-Score:-8.02|P-value:5.36E-16||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:SYN8(YAL014C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TAD1(YGL243W)|FD-Score:-6.6|P-value:1.99E-11||SGD DESC:tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala Gene:TAX4(YJL083W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TDA2(YER071C_p)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TEF2(YBR118W)|FD-Score:-6.92|P-value:2.23E-12||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TGL1(YKL140W)|FD-Score:-5.57|P-value:1.25E-8||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:TIR1(YER011W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TIR2(YOR010C)|FD-Score:-5.72|P-value:5.27E-9||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:TKL1(YPR074C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA17(YDL110C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TPA1(YER049W)|FD-Score:-3.84|P-value:6.17E-5||SGD DESC:Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate Gene:TRP3(YKL211C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSA1(YML028W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:UBP6(YFR010W)|FD-Score:-3.39|P-value:3.49E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VHR2(YER064C_p)|FD-Score:-4.84|P-value:6.63E-7||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VPS21(YOR089C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS5(YOR069W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:WHI4(YDL224C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:WHI5(YOR083W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 Gene:YBL036C(YBL036C)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL011C(YDL011C_d)|FD-Score:-3.75|P-value:8.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR491C(YDR491C_d)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEA4(YEL004W)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER Gene:YEH1(YLL012W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:YER067C-A(YER067C-A_d)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YFL063W(YFL063W_d)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YGL081W(YGL081W_p)|FD-Score:-5.22|P-value:8.84E-8||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGL149W(YGL149W_d)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGL218W(YGL218W_d)|FD-Score:4.36|P-value:6.65E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR127W(YGR127W_p)|FD-Score:-5.98|P-value:1.13E-9||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR149W(YGR149W_p)|FD-Score:-3.75|P-value:8.78E-5||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YGR228W(YGR228W_d)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR003C(YHR003C)|FD-Score:-6.37|P-value:9.26E-11||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YJL068C(YJL068C)|FD-Score:-3.78|P-value:7.94E-5||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJL169W(YJL169W_d)|FD-Score:-6.44|P-value:5.81E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR056C(YJR056C_p)|FD-Score:-6.97|P-value:1.53E-12||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YJR142W(YJR142W_p)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YKL131W(YKL131W_d)|FD-Score:-3.87|P-value:5.50E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL151C(YKL151C)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YKR023W(YKR023W_p)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL020C(YLL020C_d)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR041W(YLR041W_d)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Gene:YLR283W(YLR283W_p)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-4.4|P-value:5.36E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YLR434C(YLR434C_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML053C(YML053C_p)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene Gene:YML083C(YML083C_p)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YML116W-A(YML116W-A_d)|FD-Score:-5.2|P-value:1.01E-7||SGD DESC:Putative protein of unknown function Gene:YMR007W(YMR007W_d)|FD-Score:-7.55|P-value:2.13E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR082C(YMR082C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR090W(YMR090W_p)|FD-Score:6.68|P-value:1.21E-11||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR099C(YMR099C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR111C(YMR111C_p)|FD-Score:-4.86|P-value:5.90E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR147W(YMR147W_p)|FD-Score:-5.78|P-value:3.74E-9||SGD DESC:Putative protein of unknown function Gene:YMR155W(YMR155W_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR182W-A(YMR182W-A_p)|FD-Score:-5.24|P-value:7.98E-8||SGD DESC:Putative protein of unknown function Gene:YMR210W(YMR210W)|FD-Score:-5.53|P-value:1.62E-8||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YNL043C(YNL043C_d)|FD-Score:4.44|P-value:4.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL303W(YNL303W_d)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Putative protein of unknown function Gene:YOR022C(YOR022C_p)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR062C(YOR062C_p)|FD-Score:-3.84|P-value:6.06E-5||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR300W(YOR300W_d)|FD-Score:-3.9|P-value:4.71E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOR345C(YOR345C_d)|FD-Score:-7.63|P-value:1.14E-14||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL107W(YPL107W_p)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL245W(YPL245W_p)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YRA2(YKL214C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YVC1(YOR087W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL069C_d3.344.14E-40.25YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YDR081C3.099.98E-40.10PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YLR051C2.990.001400.06FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YPR086W2.930.001670.07SUA7Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
YOR294W2.860.002100.12RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YDL058W2.750.003010.10USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YBR202W2.640.004100.01MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YLR230W_d2.640.004200.05YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YKL042W2.580.004938.33E-4SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YGR120C2.580.004940.01COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL030W2.570.005060.19RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YPL012W2.380.008570.02RRP12Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
YPR165W2.360.009130.07RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YPL217C2.290.011000.12BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YOR278W2.170.014900.10HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR090W_p6.681.21E-11YMR090W_pPutative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
YPR074C5.181.09E-7TKL1Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
YHR030C5.171.15E-7SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YNR057C4.759.94E-7BIO4Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels
YLR441C4.444.49E-6RPS1ARibosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication
YNL043C_d4.444.59E-6YNL043C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
YOR188W4.405.46E-6MSB1Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGL218W_d4.366.65E-6YGL218W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance
YKL211C4.111.97E-5TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL251C4.112.01E-5HFM1Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
YGR228W_d4.102.03E-5YGR228W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
YER161C4.072.34E-5SPT2Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins
YGL078C3.953.94E-5DBP3RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype
YLR077W3.701.08E-4FMP25Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria
YLR185W3.661.26E-4RPL37ARibosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_750
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3366.48E-156SGTC_1107amlodipine 47.0 μMNIH Clinical Collection21620.164835
0.2486.94E-83SGTC_6133914-0030 117.0 μMChemDiv (Drug-like library)11453950.125
0.1271.24E-22SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.181818DNA intercalators
0.1224.02E-21SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0724638DNA intercalators
0.1111.61E-17SGTC_1947st076332 61.6 μMTimTec (Natural product derivative library)242084160.142857
0.0992.46E-14SGTC_6140462-0418 107.0 μMChemDiv (Drug-like library)51883030.112676
0.0976.64E-14SGTC_6304356-0227 183.0 μMChemDiv (Drug-like library)7793120.0769231
0.0936.82E-13SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0519481DNA intercalators
0.0822.57E-10SGTC_166k072-0058 166.4 μMChemDiv (Drug-like library)58462290.060241DNA intercalators
0.0765.83E-9SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.0923077redox potentiating
0.0731.83E-8SGTC_1943st076331 65.2 μMTimTec (Natural product derivative library)242084150.164557
0.0715.03E-8SGTC_1349flufenamic acid 69.0 μMChemDiv (Drug-like library)33710.0779221
0.0672.17E-7SGTC_9801473-0395 201.0 μMChemDiv (Drug-like library)7688760.164384
0.0672.72E-7SGTC_1614st002383 48.6 μMTimTec (Natural product derivative library)19472350.190476cell wall signaling
0.0663.29E-7SGTC_74000s-0487 160.3 μMChemDiv (Drug-like library)6775480.185714

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1631benzylvanillin81.9 μM0.42105375506TimTec (Natural product derivative library)242.269863.1403Golgi
SGTC_1708st03323431.83 μM0.307692674664TimTec (Natural product derivative library)321.165923.88803
SGTC_2790542397071.43 μM0.31376240Chembridge (Drug-like library)389.486785.09604endomembrane recycling
SGTC_1717st03314358.04 μM0.294118674616TimTec (Natural product derivative library)294.7053834.0104
SGTC_2797785909881.82 μM0.289855972681Chembridge (Drug-like library)342.802743.87805
SGTC_2870903852812.99 μM0.2835826463316Chembridge (Drug-like library)325.810443.17804
SGTC_1714st03323613.63 μM0.2676062916566TimTec (Natural product derivative library)355.610984.55203
SGTC_14033909-781562.9 μM0.2666676615483ChemDiv (Drug-like library)389.4222035.11216
SGTC_3333486-013140.1 μM0.2647066744690ChemDiv (Drug-like library)296.320541.31525amide catabolism
SGTC_3123912616249.47 μM0.26315825239884Chembridge (Drug-like library)367.424883.32107