0833-0568

[(E)-1-(3-oxobenzo[f]chromen-2-yl)ethylideneamino]thiourea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_751
Screen concentration 350.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6954252
SMILES CC(=NNC(=S)N)C1=CC2=C(C=CC3=CC=CC=C32)OC1=O
Standardized SMILES CC(=NNC(=S)N)C1=Cc2c(OC1=O)ccc3ccccc23
Molecular weight 311.3583
ALogP 2.71
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.53
% growth inhibition (Hom. pool) 4.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6954252
Download HIP data (tab-delimited text)  (excel)
Gene:BOS1(YLR078C)|FD-Score:-3.54|P-value:2.03E-4|Clearance:0||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CEG1(YGL130W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.09||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:COG4(YPR105C)|FD-Score:-3.19|P-value:7.05E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:MCM10(YIL150C)|FD-Score:3.9|P-value:4.89E-5|Clearance:0.33||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MNP1(YGL068W)|FD-Score:-3.95|P-value:3.90E-5|Clearance:0||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:RPA135(YPR010C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.16||SGD DESC:RNA polymerase I second largest subunit A135 Gene:SED5(YLR026C)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.35||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TOM22(YNL131W)|FD-Score:-3.19|P-value:7.06E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:BOS1(YLR078C)|FD-Score:-3.54|P-value:2.03E-4|Clearance:0||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CEG1(YGL130W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.09||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:COG4(YPR105C)|FD-Score:-3.19|P-value:7.05E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:MCM10(YIL150C)|FD-Score:3.9|P-value:4.89E-5|Clearance:0.33||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MNP1(YGL068W)|FD-Score:-3.95|P-value:3.90E-5|Clearance:0||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:RPA135(YPR010C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.16||SGD DESC:RNA polymerase I second largest subunit A135 Gene:SED5(YLR026C)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.35||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TOM22(YNL131W)|FD-Score:-3.19|P-value:7.06E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6954252
Download HOP data (tab-delimited text)  (excel)
Gene:AIM21(YIR003W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:AIM24(YJR080C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:AIM29(YKR074W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALF1(YNL148C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASI2(YNL159C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATG2(YNL242W)|FD-Score:5.97|P-value:1.18E-9||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:CHD1(YER164W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CMC4(YMR194C-B)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CTF18(YMR078C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTK1(YKL139W)|FD-Score:-3.54|P-value:1.96E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CUE5(YOR042W)|FD-Score:-5.46|P-value:2.32E-8||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DSE3(YOR264W)|FD-Score:5.76|P-value:4.26E-9||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUN1(YDL101C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EKI1(YDR147W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:FUB1(YCR076C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV12(YOR183W_p)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAD1(YMR250W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCV3(YAL044C)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GYL1(YMR192W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:IRC2(YDR112W_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:JLP1(YLL057C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:KIN4(YOR233W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LDB18(YLL049W)|FD-Score:3.77|P-value:8.00E-5||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:MAC1(YMR021C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDL1(YLR188W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MND2(YIR025W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPS17(YMR188C)|FD-Score:-3.89|P-value:4.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MTC3(YGL226W)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NPR1(YNL183C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NUP120(YKL057C)|FD-Score:4.32|P-value:7.83E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OCA1(YNL099C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:POS5(YPL188W)|FD-Score:3.83|P-value:6.34E-5||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PPR1(YLR014C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PRM1(YNL279W)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:RAD18(YCR066W)|FD-Score:5.03|P-value:2.49E-7||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RPL12A(YEL054C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL1A(YPL220W)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL21B(YPL079W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL37A(YLR185W)|FD-Score:5.33|P-value:4.79E-8||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RPL9B(YNL067W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS14A(YCR031C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RTS3(YGR161C_p)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAC3(YDR159W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SMA1(YPL027W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNF8(YPL002C)|FD-Score:-3.76|P-value:8.64E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SRY1(YKL218C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:TAX4(YJL083W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TDA6(YPR157W_p)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TRE1(YPL176C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VTA1(YLR181C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YAR029W(YAR029W_p)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL071C(YBL071C_d)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Putative protein of unknown function Gene:YCP4(YCR004C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR115W(YDR115W_p)|FD-Score:-5.25|P-value:7.50E-8||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR209C(YDR209C_d)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YGL015C(YGL015C_p)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGR266W(YGR266W)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YKL053W(YKL053W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKL069W(YKL069W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLL059C(YLL059C_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR345W(YLR345W_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YMR007W(YMR007W_d)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:4.98|P-value:3.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL107W(YOL107W_p)|FD-Score:4.94|P-value:4.00E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YPT10(YBR264C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:AIM21(YIR003W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:AIM24(YJR080C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:AIM29(YKR074W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALF1(YNL148C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ASI2(YNL159C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATG2(YNL242W)|FD-Score:5.97|P-value:1.18E-9||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:CHD1(YER164W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CMC4(YMR194C-B)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CTF18(YMR078C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTK1(YKL139W)|FD-Score:-3.54|P-value:1.96E-4||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:CUE5(YOR042W)|FD-Score:-5.46|P-value:2.32E-8||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DSE3(YOR264W)|FD-Score:5.76|P-value:4.26E-9||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUN1(YDL101C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EKI1(YDR147W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:FUB1(YCR076C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV12(YOR183W_p)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAD1(YMR250W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCV3(YAL044C)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GYL1(YMR192W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:IRC2(YDR112W_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:JLP1(YLL057C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:KIN4(YOR233W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LDB18(YLL049W)|FD-Score:3.77|P-value:8.00E-5||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:MAC1(YMR021C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDL1(YLR188W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MND2(YIR025W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPS17(YMR188C)|FD-Score:-3.89|P-value:4.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MTC3(YGL226W)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NPR1(YNL183C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NUP120(YKL057C)|FD-Score:4.32|P-value:7.83E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OCA1(YNL099C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:POS5(YPL188W)|FD-Score:3.83|P-value:6.34E-5||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PPR1(YLR014C)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p Gene:PRM1(YNL279W)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:RAD18(YCR066W)|FD-Score:5.03|P-value:2.49E-7||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RPL12A(YEL054C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL1A(YPL220W)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL21B(YPL079W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL24A(YGL031C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPL37A(YLR185W)|FD-Score:5.33|P-value:4.79E-8||SGD DESC:Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication Gene:RPL9B(YNL067W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS14A(YCR031C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RTS3(YGR161C_p)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAC3(YDR159W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SMA1(YPL027W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNF8(YPL002C)|FD-Score:-3.76|P-value:8.64E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SRY1(YKL218C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:TAX4(YJL083W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TDA6(YPR157W_p)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TRE1(YPL176C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VTA1(YLR181C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YAR029W(YAR029W_p)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL071C(YBL071C_d)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Putative protein of unknown function Gene:YCP4(YCR004C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR115W(YDR115W_p)|FD-Score:-5.25|P-value:7.50E-8||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR209C(YDR209C_d)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YGL015C(YGL015C_p)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGR266W(YGR266W)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YKL053W(YKL053W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YKL069W(YKL069W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YLL059C(YLL059C_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR345W(YLR345W_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YMR007W(YMR007W_d)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:4.98|P-value:3.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL107W(YOL107W_p)|FD-Score:4.94|P-value:4.00E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YPT10(YBR264C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR026C4.241.11E-50.35SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YIL150C3.904.89E-50.33MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YPR010C3.561.83E-40.16RPA135RNA polymerase I second largest subunit A135
YGL130W3.403.33E-40.09CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YAL034C-B_d3.314.69E-40.25YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGR175C3.060.001120.02ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YOL135C3.030.001200.17MED7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YLR051C2.870.002070.05FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YMR146C2.820.002430.02TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YNL137C2.800.002590.00NAM9Mitochondrial ribosomal component of the small subunit
YNL282W2.790.002600.02POP3Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL012W2.770.002810.04PRP40U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
YDR236C2.730.003160.02FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YFL017C2.710.003330.01GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YAL034W-A2.700.003460.01MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL242W5.971.18E-9ATG2Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
YOR264W5.764.26E-9DSE3Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDR495C5.344.71E-8VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YLR185W5.334.79E-8RPL37ARibosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication
YCR066W5.032.49E-7RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YNL235C_d4.983.23E-7YNL235C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
YNL099C4.963.51E-7OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YOL107W_p4.944.00E-7YOL107W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein
YPL079W4.731.11E-6RPL21BRibosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
YIR025W4.444.41E-6MND2Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
YKL057C4.327.83E-6NUP120Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
YNL159C4.251.06E-5ASI2Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
YNL183C4.221.23E-5NPR1Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
YMR194C-B4.181.48E-5CMC4Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
YOR233W4.161.62E-5KIN4Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_751
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0594.80E-6SGTC_12460422-0085 105.0 μMChemDiv (Drug-like library)2642950.114754
0.0561.67E-5SGTC_31249127567 49.5 μMChembridge (Drug-like library)175920300.0714286DNA damage response
0.0518.85E-5SGTC_10451348-1485 165.0 μMChemDiv (Drug-like library)68106140.1
0.0519.59E-5SGTC_1936st074407 74.3 μMTimTec (Natural product derivative library)8098130.103896DNA damage response
0.0501.04E-4SGTC_32529135871 49.5 μMChembridge (Drug-like library)176828380.135135
0.0501.19E-4SGTC_3234013-0906 34.3 μMChemDiv (Drug-like library)29100860.0659341ERG2
0.0491.76E-4SGTC_9631269-2853 151.0 μMChemDiv (Drug-like library)36682230.114286
0.0482.48E-4SGTC_32579139206 33.0 μMChembridge (Drug-like library)49100410.0487805
0.0482.58E-4SGTC_20124023501 200.0 μMChembridge (Fragment library)34774600.09375
0.0472.64E-4SGTC_1938st074717 56.4 μMTimTec (Natural product derivative library)172509790.109756
0.0464.09E-4SGTC_1871st057448 67.3 μMTimTec (Natural product derivative library)6917470.238806
0.0464.16E-4SGTC_395anisomycin 6.2 μMICCB bioactive library66103090.123288
0.0464.59E-4SGTC_7461095-0025 46.6 μMChemDiv (Drug-like library)67492860.0519481RSC & ERG11
0.0456.17E-4SGTC_25354',4'-dimethoxydalbergione 51.2 μMMicrosource (Natural product library)3641060.0958904DNA damage response
0.0456.22E-4SGTC_31149122766 49.5 μMChembridge (Drug-like library)171769850.125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1220236-0016402.4 μM0.4411766808838ChemDiv (Drug-like library)361.458523.09835RNA processing & uracil transport
SGTC_8810833-0569170 μM0.4406785418700ChemDiv (Drug-like library)306.315362.68414
SGTC_2898905627058.44 μM0.43939419154699Chembridge (Drug-like library)352.38381.80215
SGTC_2435hr heat shock (37°C) + splitomicin1 μM0.3035715269Miscellaneous198.21732.83102heat shock/prefoldin
SGTC_424splitomicin100 mM0.3035715269Miscellaneous198.21732.83102
SGTC_1859st05599820 μM0.285714676301TimTec (Natural product derivative library)288.296783.80213
SGTC_1842st05536261.2 μM0.272727676032TimTec (Natural product derivative library)304.296183.5624
SGTC_1821st05346565.6 μM0.269841675086TimTec (Natural product derivative library)282.090121.81613
SGTC_1628st00425277.7 μM0.261538692968TimTec (Natural product derivative library)257.28452.4713
SGTC_22047233102200 μM0.2615382172480Chembridge (Fragment library)229.231341.6841360S ribosome export