1322-0200

2-hydroxy-N-[(Z)-(5-nitrofuran-2-yl)methylideneamino]benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_753
Screen concentration 289.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5403907
SMILES C1=CC=C(C(=C1)C(=O)NN=CC2=CC=C(O2)[N+](=O)[O-])O
Standardized SMILES Oc1ccccc1C(=O)NN=Cc2oc(cc2)[N+](=O)[O-]
Molecular weight 275.217
ALogP 1.86
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.28
% growth inhibition (Hom. pool) 5.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5403907
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCA1(YER168C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.12||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CHS2(YBR038W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:GLN4(YOR168W)|FD-Score:3.39|P-value:3.47E-4|Clearance:0.37||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:IQG1(YPL242C)|FD-Score:-3.76|P-value:8.53E-5|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KRR1(YCL059C)|FD-Score:4.87|P-value:5.49E-7|Clearance:1.13||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:MPS3(YJL019W)|FD-Score:3.74|P-value:9.15E-5|Clearance:0.23||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:RPL10(YLR075W)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPP0(YLR340W)|FD-Score:-4.36|P-value:6.38E-6|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC20(YDR498C)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:ARC40(YBR234C)|FD-Score:-3.52|P-value:2.19E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CCA1(YER168C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.12||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CHS2(YBR038W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:GLN4(YOR168W)|FD-Score:3.39|P-value:3.47E-4|Clearance:0.37||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:IQG1(YPL242C)|FD-Score:-3.76|P-value:8.53E-5|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KRR1(YCL059C)|FD-Score:4.87|P-value:5.49E-7|Clearance:1.13||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:MPS3(YJL019W)|FD-Score:3.74|P-value:9.15E-5|Clearance:0.23||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:RPL10(YLR075W)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPP0(YLR340W)|FD-Score:-4.36|P-value:6.38E-6|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC20(YDR498C)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5403907
Download HOP data (tab-delimited text)  (excel)
Gene:AIR2(YDL175C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:5.51|P-value:1.78E-8||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG17(YLR423C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BCK2(YER167W)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BST1(YFL025C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CGI121(YML036W)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COX14(YML129C)|FD-Score:-4.08|P-value:2.28E-5||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CUE5(YOR042W)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ECT1(YGR007W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ERT1(YBR239C_p)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:FIG4(YNL325C)|FD-Score:-3.85|P-value:5.80E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FLO11(YIR019C)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FUB1(YCR076C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:GCN20(YFR009W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GDH1(YOR375C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTT2(YLL060C)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HIT1(YJR055W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IRC2(YDR112W_d)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITR2(YOL103W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:MAE1(YKL029C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MET32(YDR253C)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MRPL23(YOR150W)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MXR2(YCL033C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:PMS1(YNL082W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAD18(YCR066W)|FD-Score:6|P-value:1.00E-9||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RBK1(YCR036W)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Putative ribokinase Gene:RCK2(YLR248W)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RDL2(YOR286W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RGI2(YIL057C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RHO2(YNL090W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RKM5(YLR137W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RPL21B(YPL079W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RSR1(YGR152C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAS4(YDR181C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SCY1(YGL083W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SEG1(YMR086W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SNF2(YOR290C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPG1(YGR236C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:STF1(YDL130W-A)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA8(YAL064C-A_p)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TDH1(YJL052W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TRM12(YML005W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRS85(YDR108W)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VAM10(YOR068C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:WSS1(YHR134W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBR284W(YBR284W_p)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YBT1(YLL048C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR186C(YDR186C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YER079W(YER079W_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function Gene:YIL055C(YIL055C_p)|FD-Score:5.27|P-value:6.88E-8||SGD DESC:Putative protein of unknown function Gene:YJR056C(YJR056C_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR070W(YKR070W_p)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL020C(YLL020C_d)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLL044W(YLL044W_d)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YML084W(YML084W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR099C(YMR099C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL035C(YNL035C_p)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YPK1(YKL126W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YUH1(YJR099W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p Gene:AIR2(YDL175C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:5.51|P-value:1.78E-8||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG17(YLR423C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BCK2(YER167W)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BST1(YFL025C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CGI121(YML036W)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COX14(YML129C)|FD-Score:-4.08|P-value:2.28E-5||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CUE5(YOR042W)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ECT1(YGR007W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ERT1(YBR239C_p)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:FIG4(YNL325C)|FD-Score:-3.85|P-value:5.80E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FLO11(YIR019C)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FUB1(YCR076C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:GCN20(YFR009W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GDH1(YOR375C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTT2(YLL060C)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HIT1(YJR055W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IRC2(YDR112W_d)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITR2(YOL103W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:MAE1(YKL029C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MET32(YDR253C)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MRPL23(YOR150W)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MXR2(YCL033C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:PMS1(YNL082W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAD18(YCR066W)|FD-Score:6|P-value:1.00E-9||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RBK1(YCR036W)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Putative ribokinase Gene:RCK2(YLR248W)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RDL2(YOR286W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:RGI2(YIL057C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RHO2(YNL090W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RKM5(YLR137W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species Gene:RPL21B(YPL079W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RRI1(YDL216C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:RSR1(YGR152C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAS4(YDR181C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SCY1(YGL083W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SEG1(YMR086W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SNF2(YOR290C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPG1(YGR236C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:STF1(YDL130W-A)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA8(YAL064C-A_p)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TDH1(YJL052W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TRM12(YML005W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRS85(YDR108W)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VAM10(YOR068C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:WSS1(YHR134W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBR284W(YBR284W_p)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YBT1(YLL048C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR186C(YDR186C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YER079W(YER079W_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function Gene:YIL055C(YIL055C_p)|FD-Score:5.27|P-value:6.88E-8||SGD DESC:Putative protein of unknown function Gene:YJR056C(YJR056C_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR070W(YKR070W_p)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL020C(YLL020C_d)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLL044W(YLL044W_d)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YML084W(YML084W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR099C(YMR099C)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL035C(YNL035C_p)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YPK1(YKL126W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YUH1(YJR099W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCL059C4.875.49E-71.13KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YJL019W3.749.15E-50.23MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YER168C3.512.22E-40.12CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YOR168W3.393.47E-40.37GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YDL132W3.020.001250.02CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YHR069C3.000.001340.28RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YMR211W2.730.003190.14DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YJR076C2.590.004810.03CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YGR065C2.560.005210.04VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YLR129W2.520.005840.01DIP2Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
YDR325W2.510.006070.02YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YOR256C2.480.006510.06TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YAR019C2.420.007700.02CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YGR103W2.400.008200.04NOP7Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
YOR057W2.360.009180.02SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR066W6.001.00E-9RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YPL061W5.511.78E-8ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YIL055C_p5.276.88E-8YIL055C_pPutative protein of unknown function
YCR036W4.934.03E-7RBK1Putative ribokinase
YBR284W_p4.631.86E-6YBR284W_pPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
YLL020C_d4.562.61E-6YLL020C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1
YDR253C4.483.66E-6MET32Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication
YJR090C4.454.35E-6GRR1F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification
YLR248W4.444.46E-6RCK2Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
YDR112W_d4.308.63E-6IRC2_dDubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci
YDL175C4.251.09E-5AIR2RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication
YGR007W4.221.23E-5ECT1Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases
YLR137W4.141.75E-5RKM5Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species
YKL029C4.062.43E-5MAE1Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
YOR290C4.062.47E-5SNF2Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p

GO enrichment analysis for SGTC_753
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0814.17E-10SGTC_20315180455 200.0 μMChembridge (Fragment library)299490.0384615
0.0741.35E-8SGTC_5504123-0385 25.7 μMChemDiv (Drug-like library)57311230.308824TSC3-RPN4
0.0681.51E-7SGTC_24835729050 27.2 μMMiscellaneous28651800.1375
0.0664.29E-7SGTC_25354',4'-dimethoxydalbergione 51.2 μMMicrosource (Natural product library)3641060.0694444DNA damage response
0.0647.88E-7SGTC_22277312221 64.1 μMChembridge (Fragment library)8871880.134328
0.0649.60E-7SGTC_1936st074407 74.3 μMTimTec (Natural product derivative library)8098130.0649351DNA damage response
0.0612.49E-6SGTC_22997976368 114.9 μMChembridge (Fragment library)29797180.0895522
0.0596.00E-6SGTC_391gliotoxin 8.3 μMMiscellaneous62230.0465116
0.0588.53E-6SGTC_482fipronil 114.0 μMICCB bioactive library33520.0365854
0.0589.67E-6SGTC_245methotrexate 289.6 μMMiscellaneous1269410.0978261
0.0571.06E-5SGTC_32899119912 71.4 μMChembridge (Drug-like library)170148290.107143
0.0571.17E-5SGTC_32179129673 49.5 μMChembridge (Drug-like library)172483120.0921053
0.0534.17E-5SGTC_23799035213 200.0 μMChembridge (Fragment library)6465510.0833333
0.0534.72E-5SGTC_2677mitomycin C 100.0 μMMiscellaneous57460.0449438DNA damage response
0.0535.10E-5SGTC_14254048-4433 7.3 μMChemDiv (Drug-like library)237349460.238806copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_11971601-0112267 μM0.4406787350313ChemDiv (Drug-like library)299.281422.31625
SGTC_6541124-027180.9 μM0.4310345403748ChemDiv (Drug-like library)309.115462.58524
SGTC_1700st0308407.93 μM0.4142865499307TimTec (Natural product derivative library)359.333382.92417PDR1
SGTC_12111056-003332.3 μM0.4090916741922ChemDiv (Drug-like library)324.290881.04235
SGTC_13231348-135111.2 μM0.406256989654ChemDiv (Drug-like library)336.304882.617
SGTC_30833-058795.58 μM0.4038465382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_1731st03596463 μM0.3833336739575TimTec (Natural product derivative library)317.253640.26547
SGTC_8981000-084928.3 μM0.3666676751807ChemDiv (Drug-like library)356.374042.72334
SGTC_1166k206-0078225 μM0.3650795404595ChemDiv (Drug-like library)325.749083.40214
SGTC_13051181-0477144 μM0.3559326252086ChemDiv (Drug-like library)282.337023.39423