0508-1999

6-[(2-benzoyl-4-methylanilino)methylidene]-4-bromocyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_756
Screen concentration 28.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 6753853
SMILES CC1=CC(=C(C=C1)NC=C2C=C(C=CC2=O)Br)C(=O)C3=CC=CC=C3
Standardized SMILES Cc1ccc(N=Cc2cc(Br)ccc2O)c(c1)C(=O)c3ccccc3
Molecular weight 394.2612
ALogP 4.44
H-bond donor count 1
H-bond acceptor count 3
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.19
% growth inhibition (Hom. pool) 7.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6753853
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:4.78|P-value:8.57E-7|Clearance:0.41||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC37(YDR168W)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.13||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:ERG29(YMR134W_p)|FD-Score:5.06|P-value:2.06E-7|Clearance:0.28||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GCD11(YER025W)|FD-Score:3.79|P-value:7.51E-5|Clearance:0.16||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:NIP1(YMR309C)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:OKP1(YGR179C)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.42||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PBN1(YCL052C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.05||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RAP1(YNL216W)|FD-Score:-4.04|P-value:2.71E-5|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPC17(YJL011C)|FD-Score:3.12|P-value:8.94E-4|Clearance:0.06||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:7.1|P-value:6.46E-13|Clearance:1.22||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRB1(YMR131C)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP46(YGR095C)|FD-Score:3.83|P-value:6.40E-5|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC31(YDL195W)|FD-Score:4.37|P-value:6.18E-6|Clearance:0.02||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SSY5(YJL156C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SUA5(YGL169W)|FD-Score:3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI3(YPL237W)|FD-Score:4.35|P-value:6.80E-6|Clearance:0.01||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF34(YMR146C)|FD-Score:11.4|P-value:2.54E-30|Clearance:4.29||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:5.88|P-value:2.09E-9|Clearance:0.81||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS23(YDR246W)|FD-Score:3.14|P-value:8.57E-4|Clearance:0.01||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YBL077W(YBL077W_d)|FD-Score:3.63|P-value:1.39E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YOR146W(YOR146W_d)|FD-Score:4.34|P-value:7.15E-6|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR136C(YPR136C_d)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:APC5(YOR249C)|FD-Score:4.78|P-value:8.57E-7|Clearance:0.41||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC37(YDR168W)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.13||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:ERG29(YMR134W_p)|FD-Score:5.06|P-value:2.06E-7|Clearance:0.28||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:GCD11(YER025W)|FD-Score:3.79|P-value:7.51E-5|Clearance:0.16||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:NIP1(YMR309C)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:OKP1(YGR179C)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.42||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PBN1(YCL052C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.05||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RAP1(YNL216W)|FD-Score:-4.04|P-value:2.71E-5|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPC17(YJL011C)|FD-Score:3.12|P-value:8.94E-4|Clearance:0.06||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:7.1|P-value:6.46E-13|Clearance:1.22||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRB1(YMR131C)|FD-Score:-3.29|P-value:5.06E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRP46(YGR095C)|FD-Score:3.83|P-value:6.40E-5|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC31(YDL195W)|FD-Score:4.37|P-value:6.18E-6|Clearance:0.02||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SSY5(YJL156C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SUA5(YGL169W)|FD-Score:3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI3(YPL237W)|FD-Score:4.35|P-value:6.80E-6|Clearance:0.01||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF34(YMR146C)|FD-Score:11.4|P-value:2.54E-30|Clearance:4.29||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:5.88|P-value:2.09E-9|Clearance:0.81||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS23(YDR246W)|FD-Score:3.14|P-value:8.57E-4|Clearance:0.01||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YBL077W(YBL077W_d)|FD-Score:3.63|P-value:1.39E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YOR146W(YOR146W_d)|FD-Score:4.34|P-value:7.15E-6|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL238C(YPL238C_d)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR136C(YPR136C_d)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6753853
Download HOP data (tab-delimited text)  (excel)
Gene:ADD66(YKL206C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:APE2(YKL157W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:AZF1(YOR113W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BDS1(YOL164W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BNA3(YJL060W)|FD-Score:4.32|P-value:7.64E-6||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC2(YDR275W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BUD26(YDR241W_d)|FD-Score:5|P-value:2.84E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:DNF1(YER166W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ERG4(YGL012W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA4(YMR246W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FKH1(YIL131C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FUM1(YPL262W)|FD-Score:-3.78|P-value:7.77E-5||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GET2(YER083C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GND2(YGR256W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GUT2(YIL155C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HPF1(YOL155C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HUG1(YML058W-A)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:KGD2(YDR148C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:MAL33(YBR297W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MET17(YLR303W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MNN2(YBR015C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP21(YBL090W)|FD-Score:6.37|P-value:9.76E-11||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MSL1(YIR009W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MSS18(YPR134W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NSI1(YDR026C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:OCH1(YGL038C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:ORM2(YLR350W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PFK1(YGR240C)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGC1(YPL206C)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO5(YBR093C)|FD-Score:5.28|P-value:6.38E-8||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:POP2(YNR052C)|FD-Score:5.77|P-value:4.05E-9||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RTS1(YOR014W)|FD-Score:6.66|P-value:1.40E-11||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT109(YLL002W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SBH1(YER087C-B)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SDH4(YDR178W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SER3(YER081W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SHE9(YDR393W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SIP2(YGL208W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SIP4(YJL089W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SNX4(YJL036W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPS19(YNL202W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SRY1(YKL218C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSD1(YDR293C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWT1(YOR166C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TGL3(YMR313C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TIF1(YKR059W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIP41(YPR040W)|FD-Score:4.91|P-value:4.50E-7||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRM2(YKR056W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:UAF30(YOR295W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:4.38|P-value:6.07E-6||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBP9(YER098W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VMA8(YEL051W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS51(YKR020W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS72(YDR485C)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAF9(YNL107W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YAL018C(YAL018C_p)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Putative protein of unknown function Gene:YBR284W(YBR284W_p)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR061W(YCR061W_p)|FD-Score:5.62|P-value:9.57E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YGR250C(YGR250C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHC3(YJL059W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIL025C(YIL025C_d)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL222C(YKL222C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function Gene:YMR085W(YMR085W_p)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR144W(YMR144W_p)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YMR245W(YMR245W_d)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL266W(YNL266W_d)|FD-Score:-3.16|P-value:7.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YNR025C(YNR025C_d)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR114W(YOR114W_p)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPR064W(YPR064W_d)|FD-Score:4.32|P-value:7.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR126C(YPR126C_d)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YTA12(YMR089C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:YVH1(YIR026C)|FD-Score:5.38|P-value:3.75E-8||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ADD66(YKL206C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:APE2(YKL157W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:AZF1(YOR113W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BDS1(YOL164W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BNA3(YJL060W)|FD-Score:4.32|P-value:7.64E-6||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC2(YDR275W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BUD26(YDR241W_d)|FD-Score:5|P-value:2.84E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:DNF1(YER166W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ERG4(YGL012W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA4(YMR246W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FKH1(YIL131C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:FUM1(YPL262W)|FD-Score:-3.78|P-value:7.77E-5||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GET2(YER083C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GND2(YGR256W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GUT2(YIL155C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HPF1(YOL155C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HUG1(YML058W-A)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:KGD2(YDR148C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:MAL33(YBR297W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MET17(YLR303W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MNN2(YBR015C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP21(YBL090W)|FD-Score:6.37|P-value:9.76E-11||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MSL1(YIR009W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MSS18(YPR134W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NSI1(YDR026C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:OCH1(YGL038C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:ORM2(YLR350W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PFK1(YGR240C)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGC1(YPL206C)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO5(YBR093C)|FD-Score:5.28|P-value:6.38E-8||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:POP2(YNR052C)|FD-Score:5.77|P-value:4.05E-9||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RTS1(YOR014W)|FD-Score:6.66|P-value:1.40E-11||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT109(YLL002W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SBH1(YER087C-B)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SDH4(YDR178W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SER3(YER081W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SHE9(YDR393W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SIP2(YGL208W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SIP4(YJL089W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SNX4(YJL036W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPS19(YNL202W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SRY1(YKL218C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSD1(YDR293C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWT1(YOR166C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TGL3(YMR313C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TIF1(YKR059W)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIP41(YPR040W)|FD-Score:4.91|P-value:4.50E-7||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRM2(YKR056W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:UAF30(YOR295W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:4.38|P-value:6.07E-6||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBP9(YER098W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VMA8(YEL051W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS51(YKR020W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS72(YDR485C)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAF9(YNL107W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YAL018C(YAL018C_p)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Putative protein of unknown function Gene:YBR284W(YBR284W_p)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR061W(YCR061W_p)|FD-Score:5.62|P-value:9.57E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YGR250C(YGR250C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHC3(YJL059W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YIL025C(YIL025C_d)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL222C(YKL222C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function Gene:YMR085W(YMR085W_p)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR144W(YMR144W_p)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YMR245W(YMR245W_d)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL266W(YNL266W_d)|FD-Score:-3.16|P-value:7.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YNR025C(YNR025C_d)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR114W(YOR114W_p)|FD-Score:3.9|P-value:4.91E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPR064W(YPR064W_d)|FD-Score:4.32|P-value:7.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR126C(YPR126C_d)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YTA12(YMR089C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:YVH1(YIR026C)|FD-Score:5.38|P-value:3.75E-8||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR146C11.402.54E-304.29TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YHR062C7.106.46E-131.22RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR429C5.882.09E-90.81TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YMR134W_p5.062.06E-70.28ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YOR249C4.788.57E-70.41APC5Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
YDL195W4.376.18E-60.02SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPL237W4.356.80E-60.01SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YOR146W_d4.347.15E-60.09YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YGR179C4.251.07E-50.42OKP1Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7
YGR095C3.836.40E-50.03RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YMR309C3.807.25E-50.01NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YER025W3.797.51E-50.16GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YBL077W_d3.631.39E-40.11YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YPL238C_d3.522.12E-40.14YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YDR168W3.383.61E-40.13CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR014W6.661.40E-11RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YBL090W6.379.76E-11MRP21Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
YNR052C5.774.05E-9POP2RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
YCR061W_p5.629.57E-9YCR061W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
YIR026C5.383.75E-8YVH1Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases
YBR093C5.286.38E-8PHO5Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
YPL206C5.131.43E-7PGC1Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs
YDR241W_d5.002.84E-7BUD26_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YPR040W4.914.50E-7TIP41Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress
YMR246W4.691.35E-6FAA4Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
YER098W4.631.82E-6UBP9Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
YGR240C4.592.19E-6PFK1Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YAL018C_p4.434.80E-6YAL018C_pPutative protein of unknown function
YHR111W4.386.07E-6UBA4Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
YJL060W4.327.64E-6BNA3Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate

GO enrichment analysis for SGTC_756
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1904.85E-49SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.27027
0.1402.25E-27SGTC_3301488-0319 80.1 μMChemDiv (Drug-like library)68466140.40625RPP1 & pyrimidine depletion
0.1395.16E-27SGTC_7700389-1121 67.2 μMChemDiv (Drug-like library)67531080.186047RPP1 & pyrimidine depletion
0.1255.99E-22SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.40625RPP1 & pyrimidine depletion
0.1151.24E-18SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.109756RPP1 & pyrimidine depletion
0.1062.72E-16SGTC_7323970-1727 52.7 μMChemDiv (Drug-like library)12756990.0888889
0.1055.65E-16SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.234375
0.0961.83E-13SGTC_6671498-1044 59.0 μMChemDiv (Drug-like library)67399820.322581RPP1 & pyrimidine depletion
0.0881.22E-11SGTC_1647st011722 22.4 μMTimTec (Natural product derivative library)28547830.138889
0.0881.42E-11SGTC_1644st010134 20.9 μMTimTec (Natural product derivative library)38181340.0945946mitochondrial processes
0.0863.01E-11SGTC_274sirtinol 40.1 μMMiscellaneous68364420.15
0.0857.56E-11SGTC_21725754775 163.9 μMChembridge (Fragment library)22633570.132353
0.0841.03E-10SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.0941176translation
0.0841.05E-10SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0958904
0.0841.23E-10SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.097561RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12700831-117017.3 μM0.7346946764716ChemDiv (Drug-like library)380.234664.25113
SGTC_310508-197457.24 μM0.7307696810525ChemDiv (Drug-like library)414.679724.6213
SGTC_130199-008765 μM0.4464294589825ChemDiv (Drug-like library)354.229326.15902ERG2
SGTC_608k048-0125159 μM0.4354846817652ChemDiv (Drug-like library)350.164082.16525
SGTC_80354-000170 μM0.4310344993152ChemDiv (Drug-like library)354.229326.15902
SGTC_13221348-107012 μM0.4307696758089ChemDiv (Drug-like library)402.308143.54224
SGTC_1320417-171525.81 μM0.406256769098ChemDiv (Drug-like library)409.299024.56114RPP1 & pyrimidine depletion
SGTC_3301488-031980.1 μM0.406256846614ChemDiv (Drug-like library)392.248663.99223RPP1 & pyrimidine depletion
SGTC_350133-005464.07 μM0.40384699677ChemDiv (Drug-like library)276.12863.23512
SGTC_8700573-221357.6 μM0.388063916321ChemDiv (Drug-like library)453.91636.78104ERG2