0433-0009

3-[2-[2-(N-acetylanilino)ethenyl]-6-ethoxycarbonyl-3-ethylbenzimidazol-3-ium-1-yl]propane-1-sulfonate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_76
Screen concentration 400.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 2841569
SMILES CC[N+]1=C(N(C2=C1C=CC(=C2)C(=O)OCC)CCCS(=O)(=O)[O-])C=CN(C3=CC=CC=C3)C(=O)C
Standardized SMILES CCOC(=O)c1ccc2c(c1)n(CCCS(=O)(=O)[O-])c(C=CN(C(=O)C)c3ccccc3)[n+]2CC
Molecular weight 499.5793
ALogP 1.02
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.56
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2841569
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.53||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CCT4(YDL143W)|FD-Score:5.75|P-value:4.49E-9|Clearance:0.53||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GPI11(YDR302W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.53||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:GPI13(YLL031C)|FD-Score:-3.9|P-value:4.82E-5|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:NOP56(YLR197W)|FD-Score:5.3|P-value:5.80E-8|Clearance:0.53||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP145(YGL092W)|FD-Score:4.89|P-value:5.05E-7|Clearance:0.53||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PRP28(YDR243C)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.53||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RPB10(YOR210W)|FD-Score:4|P-value:3.20E-5|Clearance:0.53||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RRP12(YPL012W)|FD-Score:3.74|P-value:9.36E-5|Clearance:0.53||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SEN1(YLR430W)|FD-Score:-3.11|P-value:9.40E-4|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:TIF6(YPR016C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.53||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TLG1(YDR468C)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p Gene:VRG4(YGL225W)|FD-Score:4.33|P-value:7.37E-6|Clearance:0.53||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDL016C(YDL016C_d)|FD-Score:5.21|P-value:9.21E-8|Clearance:0.53||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YGR073C(YGR073C_d)|FD-Score:4.82|P-value:7.05E-7|Clearance:0.53||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:ATM1(YMR301C)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.53||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CCT4(YDL143W)|FD-Score:5.75|P-value:4.49E-9|Clearance:0.53||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GPI11(YDR302W)|FD-Score:3.61|P-value:1.53E-4|Clearance:0.53||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:GPI13(YLL031C)|FD-Score:-3.9|P-value:4.82E-5|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:NOP56(YLR197W)|FD-Score:5.3|P-value:5.80E-8|Clearance:0.53||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP145(YGL092W)|FD-Score:4.89|P-value:5.05E-7|Clearance:0.53||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PRP28(YDR243C)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.53||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RPB10(YOR210W)|FD-Score:4|P-value:3.20E-5|Clearance:0.53||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RRP12(YPL012W)|FD-Score:3.74|P-value:9.36E-5|Clearance:0.53||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SEN1(YLR430W)|FD-Score:-3.11|P-value:9.40E-4|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:TIF6(YPR016C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.53||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TLG1(YDR468C)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p Gene:VRG4(YGL225W)|FD-Score:4.33|P-value:7.37E-6|Clearance:0.53||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDL016C(YDL016C_d)|FD-Score:5.21|P-value:9.21E-8|Clearance:0.53||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YGR073C(YGR073C_d)|FD-Score:4.82|P-value:7.05E-7|Clearance:0.53||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2841569
Download HOP data (tab-delimited text)  (excel)
Gene:AIM46(YHR199C)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BIM1(YER016W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BUD30(YDL151C_d)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCP1(YKR066C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CRF1(YDR223W)|FD-Score:4.77|P-value:9.39E-7||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CRG1(YHR209W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:ELF1(YKL160W)|FD-Score:-4.3|P-value:8.70E-6||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:FMP30(YPL103C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FRE4(YNR060W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GAT3(YLR013W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GIR2(YDR152W)|FD-Score:3.74|P-value:9.10E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLG1(YKR058W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:HIS6(YIL020C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HOT13(YKL084W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HSE1(YHL002W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IRC23(YOR044W)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IXR1(YKL032C)|FD-Score:-5.6|P-value:1.07E-8||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LYP1(YNL268W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MNT2(YGL257C)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MPD1(YOR288C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MPM1(YJL066C)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRPS9(YBR146W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC3(YGL226W)|FD-Score:4.78|P-value:8.57E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MVP1(YMR004W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NAM7(YMR080C)|FD-Score:4.77|P-value:9.35E-7||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:PAP2(YOL115W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PDH1(YPR002W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PET494(YNR045W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX1(YKL197C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:REG2(YBR050C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease Gene:RIM4(YHL024W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPA49(YNL248C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:RNA polymerase I subunit A49 Gene:RPL29(YFR032C-A)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL35A(YDL191W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:SNO2(YNL334C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SNT309(YPR101W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPO12(YHR152W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:TAL1(YLR354C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TRM732(YMR259C_p)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UPS3(YDR185C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VPS17(YOR132W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YCR099C(YCR099C_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function Gene:YIP4(YGL198W)|FD-Score:-4.48|P-value:3.66E-6||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YMR124W(YMR124W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR141C(YMR141C_d)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL228W(YNL228W_d)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPL080C(YPL080C_d)|FD-Score:7.94|P-value:1.00E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPT35(YHR105W)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:AIM46(YHR199C)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:BIM1(YER016W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BUD30(YDL151C_d)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCP1(YKR066C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CRF1(YDR223W)|FD-Score:4.77|P-value:9.39E-7||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CRG1(YHR209W)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:ELF1(YKL160W)|FD-Score:-4.3|P-value:8.70E-6||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:FMP30(YPL103C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FRE4(YNR060W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GAT3(YLR013W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GIR2(YDR152W)|FD-Score:3.74|P-value:9.10E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLG1(YKR058W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:HIS6(YIL020C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HOT13(YKL084W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HSE1(YHL002W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IRC23(YOR044W)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IXR1(YKL032C)|FD-Score:-5.6|P-value:1.07E-8||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LYP1(YNL268W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MNT2(YGL257C)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MPD1(YOR288C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MPM1(YJL066C)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRPS9(YBR146W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC3(YGL226W)|FD-Score:4.78|P-value:8.57E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MVP1(YMR004W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NAM7(YMR080C)|FD-Score:4.77|P-value:9.35E-7||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:PAP2(YOL115W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PDH1(YPR002W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PET494(YNR045W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX1(YKL197C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:REG2(YBR050C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease Gene:RIM4(YHL024W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPA49(YNL248C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:RNA polymerase I subunit A49 Gene:RPL29(YFR032C-A)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL35A(YDL191W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:SNO2(YNL334C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SNT309(YPR101W)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPO12(YHR152W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:TAL1(YLR354C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TRM732(YMR259C_p)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UPS3(YDR185C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VPS17(YOR132W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YCR099C(YCR099C_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function Gene:YIP4(YGL198W)|FD-Score:-4.48|P-value:3.66E-6||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YMR124W(YMR124W_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR141C(YMR141C_d)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL228W(YNL228W_d)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPL080C(YPL080C_d)|FD-Score:7.94|P-value:1.00E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPT35(YHR105W)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL143W5.754.49E-90.53CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR197W5.305.80E-80.53NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDL016C_d5.219.21E-80.53YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YGL092W4.895.05E-70.53NUP145Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
YGR073C_d4.827.05E-70.53YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YMR301C4.691.35E-60.53ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YGL225W4.337.37E-60.53VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YOR210W4.003.20E-50.53RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YDR243C3.807.37E-50.53PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YPL012W3.749.36E-50.53RRP12Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
YDR302W3.611.53E-40.53GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YPR016C3.591.66E-40.53TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YKL111C_d3.060.001120.11YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YDL208W2.950.001610.09NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YPL143W2.860.002150.00RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL080C_d7.941.00E-15YPL080C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL226W4.788.57E-7MTC3Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
YMR080C4.779.35E-7NAM7ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
YDR223W4.779.39E-7CRF1Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain
YHL024W4.731.12E-6RIM4Putative RNA-binding protein required for the expression of early and middle sporulation genes
YBR146W4.691.38E-6MRPS9Mitochondrial ribosomal protein of the small subunit
YJL066C4.473.83E-6MPM1Mitochondrial intermembrane space protein of unknown function
YMR141C_d4.366.55E-6YMR141C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNR060W4.211.27E-5FRE4Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
YPR002W4.211.28E-5PDH1Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
YKR058W4.151.69E-5GLG1Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication
YNL334C4.082.22E-5SNO2Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
YMR259C_p4.042.67E-5TRM732_pInteracts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene
YPL103C3.953.92E-5FMP30Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
YHR199C3.846.03E-5AIM46Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss

GO enrichment analysis for SGTC_76
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0831.95E-10SGTC_10462001-0062 216.0 μMChemDiv (Drug-like library)7397780.142857
0.0672.29E-7SGTC_22147179297 48.3 μMChembridge (Fragment library)7697440.0689655superoxide
0.0655.00E-7SGTC_2757clomiphene 4.2 μMMiscellaneous15489530.16092fatty acid desaturase (OLE1)
0.0612.66E-6SGTC_1517deoxycholic acid methyl ester 49.2 μMTimTec (Pure natural product library)2293460.0535714
0.0596.18E-6SGTC_30559094636 49.5 μMChembridge (Drug-like library)252361760.125
0.0571.34E-5SGTC_29929074591 71.4 μMChembridge (Drug-like library)177413210.122449
0.0543.47E-5SGTC_2768sulfasalazine 50.2 μMMiscellaneous53840010.11
0.0526.99E-5SGTC_15372',6'-dihydroxyflavanone 78.0 μMTimTec (Pure natural product library)51067870.0744681
0.0519.42E-5SGTC_1842st055362 61.2 μMTimTec (Natural product derivative library)6760320.0721649
0.0501.09E-4SGTC_32759138069 49.5 μMChembridge (Drug-like library)176170070.127907
0.0501.21E-4SGTC_2653dihydrofissinolide 100.0 μMMicrosource (Natural product library)68577460.0806452
0.0482.26E-4SGTC_2495cafestol 54.5 μMMicrosource (Natural product library)166676970.0458716Golgi
0.0472.88E-4SGTC_322k048-0136 19.3 μMChemDiv (Drug-like library)68232820.0891089RSC & ERG11
0.0463.82E-4SGTC_5371334-0398 209.0 μMChemDiv (Drug-like library)58469860.119565
0.0463.84E-4SGTC_30589093861 49.5 μMChembridge (Drug-like library)172174770.125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8560433-001611.3 μM0.3736263911780ChemDiv (Drug-like library)711.484362.93308
SGTC_150266-008627.1 μM0.2808991712902ChemDiv (Drug-like library)378.50725.23603endomembrane recycling
SGTC_1610st00179948.1 μM0.263736621897TimTec (Natural product derivative library)415.459543.54906
SGTC_2406ethylparaben405.7 μM0.2535218434Miscellaneous166.17391.79213
SGTC_7161683-5115658 μM0.240964723597ChemDiv (Drug-like library)303.740263.48313
SGTC_1612st00223345.4 μM0.2340431035021TimTec (Natural product derivative library)431.322924.99304
SGTC_12220366-00741.18 μM0.233766286520ChemDiv (Drug-like library)245.230741.59204TSC3-RPN4
SGTC_3047909212249.47 μM0.22727317173831Chembridge (Drug-like library)274.271961.84715
SGTC_3008907891271.43 μM0.22352916648731Chembridge (Drug-like library)287.310482.21314
SGTC_13291447-169750.6 μM0.2234042840515ChemDiv (Drug-like library)286.325722.57623