k216-0224

2,3-bis(4-fluorophenyl)quinoxaline-6-carboxylic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_761
Screen concentration 480.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1340404
SMILES C1=CC(=CC=C1C2=NC3=C(C=C(C=C3)C(=O)O)N=C2C4=CC=C(C=C4)F)F
Standardized SMILES OC(=O)c1ccc2nc(c3ccc(F)cc3)c(nc2c1)c4ccc(F)cc4
Molecular weight 362.329
ALogP 5.23
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.78
% growth inhibition (Hom. pool) 7.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1340404
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:3.78|P-value:7.77E-5|Clearance:0.01||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC16(YKL022C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.05||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC28(YBR160W)|FD-Score:4.41|P-value:5.28E-6|Clearance:0.3||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:ERG9(YHR190W)|FD-Score:3.14|P-value:8.39E-4|Clearance:0.02||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FCF1(YDR339C)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.33||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:ORC4(YPR162C)|FD-Score:3.77|P-value:8.01E-5|Clearance:0.11||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PKC1(YBL105C)|FD-Score:3.22|P-value:6.49E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RRP17(YDR412W)|FD-Score:3.13|P-value:8.88E-4|Clearance:0.01||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SEC23(YPR181C)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.08||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SMX2(YFL017W-A)|FD-Score:3.22|P-value:6.52E-4|Clearance:0.02||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPC110(YDR356W)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.07||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SPP381(YBR152W)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.06||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SPP41(YDR464W)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.16||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SSC1(YJR045C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.01||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:SUB2(YDL084W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.12||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UBA2(YDR390C)|FD-Score:6.58|P-value:2.39E-11|Clearance:1.92||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:USE1(YGL098W)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.01||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p Gene:YCG1(YDR325W)|FD-Score:6.33|P-value:1.26E-10|Clearance:1.92||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YNL150W(YNL150W_d)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:APC5(YOR249C)|FD-Score:3.78|P-value:7.77E-5|Clearance:0.01||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC16(YKL022C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.05||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC28(YBR160W)|FD-Score:4.41|P-value:5.28E-6|Clearance:0.3||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:ERG9(YHR190W)|FD-Score:3.14|P-value:8.39E-4|Clearance:0.02||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:FCF1(YDR339C)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.33||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:ORC4(YPR162C)|FD-Score:3.77|P-value:8.01E-5|Clearance:0.11||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PKC1(YBL105C)|FD-Score:3.22|P-value:6.49E-4|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RRP17(YDR412W)|FD-Score:3.13|P-value:8.88E-4|Clearance:0.01||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SEC23(YPR181C)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.08||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SMX2(YFL017W-A)|FD-Score:3.22|P-value:6.52E-4|Clearance:0.02||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPC110(YDR356W)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.07||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SPP381(YBR152W)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.06||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SPP41(YDR464W)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.16||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SSC1(YJR045C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.01||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:SUB2(YDL084W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.12||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UBA2(YDR390C)|FD-Score:6.58|P-value:2.39E-11|Clearance:1.92||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:USE1(YGL098W)|FD-Score:3.32|P-value:4.56E-4|Clearance:0.01||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p Gene:YCG1(YDR325W)|FD-Score:6.33|P-value:1.26E-10|Clearance:1.92||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YNL150W(YNL150W_d)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1340404
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ATG18(YFR021W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP14(YLR295C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BEM2(YER155C)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BLI1(YKL061W_p)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNA7(YDR428C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BPH1(YCR032W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD14(YAR014C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:COG8(YML071C)|FD-Score:-5.72|P-value:5.22E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX9(YDL067C)|FD-Score:6.55|P-value:2.93E-11||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYB5(YNL111C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:EDS1(YBR033W_p)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMC5(YIL027C)|FD-Score:-4.82|P-value:7.13E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ERG5(YMR015C)|FD-Score:4.28|P-value:9.52E-6||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAT3(YKL187C_p)|FD-Score:4.99|P-value:3.04E-7||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:GIN4(YDR507C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:HPA3(YEL066W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:LAP3(YNL239W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LIN1(YHR156C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAM33(YIL070C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MDM20(YOL076W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MID2(YLR332W)|FD-Score:4.94|P-value:3.94E-7||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MIS1(YBR084W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MMM1(YLL006W)|FD-Score:5.46|P-value:2.37E-8||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL37(YBR268W)|FD-Score:6.81|P-value:4.75E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL40(YPL173W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS1(YIR021W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSC7(YHR039C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NCS2(YNL119W)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PET111(YMR257C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET117(YER058W)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PIN2(YOR104W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:RBS1(YDL189W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:ROX3(YBL093C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL16A(YIL133C)|FD-Score:-4.01|P-value:3.09E-5||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS6A(YPL090C)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RUP1(YOR138C)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SGE1(YPR198W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLK19(YOR195W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SLS1(YLR139C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMI1(YGR229C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNA2(YDR525W-A)|FD-Score:4|P-value:3.22E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNQ2(YDR011W)|FD-Score:6.56|P-value:2.63E-11||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOV1(YMR066W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SSK1(YLR006C)|FD-Score:-5.61|P-value:9.98E-9||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TDA1(YMR291W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VIK1(YPL253C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS24(YKL041W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS75(YNL246W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WWM1(YFL010C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YAF9(YNL107W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR220C(YBR220C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YCR099C(YCR099C_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Putative protein of unknown function Gene:YDL011C(YDL011C_d)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR010C(YDR010C_d)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER134C(YER134C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR219W(YGR219W_d)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YJR084W(YJR084W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.95|P-value:3.65E-7||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR046C(YLR046C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR297W(YLR297W_p)|FD-Score:4.4|P-value:5.29E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YMC1(YPR058W)|FD-Score:4.34|P-value:7.14E-6||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YMR245W(YMR245W_d)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL013C(YNL013C_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL058C(YNL058C_p)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:ZTA1(YBR046C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:ACO1(YLR304C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ATG18(YFR021W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP14(YLR295C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BEM2(YER155C)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BLI1(YKL061W_p)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNA7(YDR428C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BPH1(YCR032W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD14(YAR014C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:COG8(YML071C)|FD-Score:-5.72|P-value:5.22E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX9(YDL067C)|FD-Score:6.55|P-value:2.93E-11||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYB5(YNL111C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:EDS1(YBR033W_p)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMC5(YIL027C)|FD-Score:-4.82|P-value:7.13E-7||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ERG5(YMR015C)|FD-Score:4.28|P-value:9.52E-6||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAT3(YKL187C_p)|FD-Score:4.99|P-value:3.04E-7||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:GIN4(YDR507C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:HPA3(YEL066W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:LAP3(YNL239W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LIN1(YHR156C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MAM33(YIL070C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MDM20(YOL076W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MID2(YLR332W)|FD-Score:4.94|P-value:3.94E-7||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MIS1(YBR084W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MMM1(YLL006W)|FD-Score:5.46|P-value:2.37E-8||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL37(YBR268W)|FD-Score:6.81|P-value:4.75E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL40(YPL173W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS1(YIR021W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:MSC7(YHR039C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NCS2(YNL119W)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PET111(YMR257C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET117(YER058W)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PIN2(YOR104W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:RBS1(YDL189W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:ROX3(YBL093C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL16A(YIL133C)|FD-Score:-4.01|P-value:3.09E-5||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS6A(YPL090C)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RUP1(YOR138C)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SGE1(YPR198W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLK19(YOR195W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SLS1(YLR139C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMI1(YGR229C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNA2(YDR525W-A)|FD-Score:4|P-value:3.22E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNQ2(YDR011W)|FD-Score:6.56|P-value:2.63E-11||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOV1(YMR066W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SSK1(YLR006C)|FD-Score:-5.61|P-value:9.98E-9||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TDA1(YMR291W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VIK1(YPL253C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VPS24(YKL041W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS75(YNL246W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WWM1(YFL010C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:YAF9(YNL107W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR220C(YBR220C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YCR099C(YCR099C_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Putative protein of unknown function Gene:YDL011C(YDL011C_d)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR010C(YDR010C_d)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER134C(YER134C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR219W(YGR219W_d)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YJR084W(YJR084W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.95|P-value:3.65E-7||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR046C(YLR046C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR297W(YLR297W_p)|FD-Score:4.4|P-value:5.29E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YMC1(YPR058W)|FD-Score:4.34|P-value:7.14E-6||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YMR245W(YMR245W_d)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL013C(YNL013C_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL058C(YNL058C_p)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:ZTA1(YBR046C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR390C6.582.39E-111.92UBA2Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YDR325W6.331.26E-101.92YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YBR160W4.415.28E-60.30CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YDR339C4.112.00E-50.33FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YOR249C3.787.77E-50.01APC5Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
YPR162C3.778.01E-50.11ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YDL084W3.661.25E-40.12SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDR464W3.551.96E-40.16SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YDR356W3.393.51E-40.07SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YGL098W3.324.56E-40.01USE1Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p
YPR181C3.314.70E-40.08SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YJR045C3.236.29E-40.01SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YBL105C3.226.49E-40.00PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YFL017W-A3.226.52E-40.02SMX2Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
YKL022C3.197.06E-40.05CDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR268W6.814.75E-12MRPL37Mitochondrial ribosomal protein of the large subunit
YDR011W6.562.63E-11SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YDL067C6.552.93E-11COX9Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YDR010C_d5.531.56E-8YDR010C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLL006W5.462.37E-8MMM1ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth
YAR014C5.072.00E-7BUD14Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
YKL187C_p4.993.04E-7FAT3_pProtein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
YKL068W-A_p4.953.65E-7YKL068W-A_pPutative protein of unknown function; identified by homology to Ashbya gossypii
YLR332W4.943.94E-7MID2O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
YLR297W_p4.405.29E-6YLR297W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
YPR058W4.347.14E-6YMC1Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication
YMR015C4.289.52E-6ERG5C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YBR220C_p4.261.03E-5YBR220C_pPutative protein of unknown function; YBR220C is not an essential gene
YPL173W4.181.45E-5MRPL40Mitochondrial ribosomal protein of the large subunit
YJR113C4.131.85E-5RSM7Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein

GO enrichment analysis for SGTC_761
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1489.12E-30SGTC_20422-0095 29.0 μMChemDiv (Drug-like library)67908400.0847458redox potentiating
0.1001.21E-14SGTC_8590439-0102 54.6 μMChemDiv (Drug-like library)6101430.127273redox potentiating
0.0912.87E-12SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)54979110.0634921calcium & mitochondrial duress
0.0913.21E-12SGTC_7621165-0429 115.0 μMChemDiv (Drug-like library)59677930.0689655TSC3-RPN4
0.0897.00E-12SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.125
0.0881.17E-11SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.116667
0.0881.18E-11SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0735294TSC3-RPN4
0.0864.41E-11SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.0810811mitochondrial stress
0.0841.28E-10SGTC_550767-0285 6.6 μMChemDiv (Drug-like library)31161660.111111
0.0831.53E-10SGTC_1340269-0018 6.9 μMChemDiv (Drug-like library)43789150.133333
0.0831.81E-10SGTC_28pentachloropyridine N-oxide 1.6 μMChemDiv (Drug-like library)1402500.0227273TSC3-RPN4
0.0813.58E-10SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.131148TRP & mitochondrial translation
0.0813.69E-10SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0923077TSC3-RPN4
0.0806.14E-10SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0754717TRP & mitochondrial translation
0.0808.67E-10SGTC_5961155-0152 116.0 μMChemDiv (Drug-like library)2803130.0909091TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1025k007-073170 μM0.3617026256118ChemDiv (Drug-like library)272.2743233.26425
SGTC_23689071271200 μM0.30434827209959Chembridge (Fragment library)190.1737831.73313
SGTC_2812797028158.44 μM0.2857142233435Chembridge (Drug-like library)371.22794.04604
SGTC_7301436-005241.2 μM0.277778768764ChemDiv (Drug-like library)288.2704233.10115mitochondrial processes
SGTC_6133914-0030117 μM0.2758621145395ChemDiv (Drug-like library)358.189383.89425
SGTC_1603st00204567.8 μM0.274515887985TimTec (Natural product derivative library)295.1357635.23602TSC3-RPN4
SGTC_2414st07723292.9 μM0.27451729801TimTec (Natural product derivative library)215.247823.13622
SGTC_3170910305449.47 μM0.27118616432970Chembridge (Drug-like library)343.3537633.8021460S ribosome export
SGTC_14494112-4002201 μM0.2666672059539ChemDiv (Drug-like library)331.3183033.1526RPP1 & pyrimidine depletion
SGTC_1036k216-021533.5 μM0.265306622330ChemDiv (Drug-like library)266.681883.90803mitochondrial response to ROS