3453-1109

3-chloro-1-(2,4-dimethylphenyl)-4-(prop-2-enylamino)pyrrole-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_763
Screen concentration 128.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1560353
SMILES CC1=CC(=C(C=C1)N2C(=O)C(=C(C2=O)Cl)NCC=C)C
Standardized SMILES Cc1ccc(N2C(=O)C(=C(NCC=C)C2=O)Cl)c(C)c1
Molecular weight 290.7448
ALogP 2.58
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.81
% growth inhibition (Hom. pool) 9.76


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1560353
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:BRR2(YER172C)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD Gene:CCT3(YJL014W)|FD-Score:4.42|P-value:4.98E-6|Clearance:0.39||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GPI1(YGR216C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.12||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:PRE6(YOL038W)|FD-Score:3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP39(YML046W)|FD-Score:3.09|P-value:9.86E-4|Clearance:0.11||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:RET2(YFR051C)|FD-Score:6.48|P-value:4.63E-11|Clearance:2.06||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPP1(YHR062C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCD5(YOR329C)|FD-Score:3.15|P-value:8.03E-4|Clearance:0.04||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:SRB4(YER022W)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STU1(YBL034C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.16||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:SUA7(YPR086W)|FD-Score:-3.69|P-value:1.13E-4|Clearance:0||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:TBF1(YPL128C)|FD-Score:3.74|P-value:9.27E-5|Clearance:0.11||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TID3(YIL144W)|FD-Score:-3.44|P-value:2.87E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YCG1(YDR325W)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.2||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YLR317W(YLR317W_d)|FD-Score:3.81|P-value:6.91E-5|Clearance:0.07||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:ZPR1(YGR211W)|FD-Score:4.03|P-value:2.75E-5|Clearance:0.02||SGD DESC:Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress Gene:BMS1(YPL217C)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:BRR2(YER172C)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.02||SGD DESC:RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD Gene:CCT3(YJL014W)|FD-Score:4.42|P-value:4.98E-6|Clearance:0.39||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GPI1(YGR216C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.12||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:PRE6(YOL038W)|FD-Score:3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP39(YML046W)|FD-Score:3.09|P-value:9.86E-4|Clearance:0.11||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:RET2(YFR051C)|FD-Score:6.48|P-value:4.63E-11|Clearance:2.06||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPP1(YHR062C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCD5(YOR329C)|FD-Score:3.15|P-value:8.03E-4|Clearance:0.04||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:SRB4(YER022W)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STU1(YBL034C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.16||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:SUA7(YPR086W)|FD-Score:-3.69|P-value:1.13E-4|Clearance:0||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:TBF1(YPL128C)|FD-Score:3.74|P-value:9.27E-5|Clearance:0.11||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TID3(YIL144W)|FD-Score:-3.44|P-value:2.87E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YCG1(YDR325W)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.2||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YLR317W(YLR317W_d)|FD-Score:3.81|P-value:6.91E-5|Clearance:0.07||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:ZPR1(YGR211W)|FD-Score:4.03|P-value:2.75E-5|Clearance:0.02||SGD DESC:Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1560353
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARF1(YDL192W)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:BUD30(YDL151C_d)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBS1(YDL069C)|FD-Score:-4.28|P-value:9.28E-6||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CIR1(YGR207C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:CLA4(YNL298W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CTS1(YLR286C)|FD-Score:4|P-value:3.18E-5||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:CYS3(YAL012W)|FD-Score:3.9|P-value:4.71E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DHH1(YDL160C)|FD-Score:5.81|P-value:3.04E-9||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DIA1(YMR316W)|FD-Score:4.96|P-value:3.45E-7||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DPL1(YDR294C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:ECM2(YBR065C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ERC1(YHR032W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FUI1(YBL042C)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAS3(YMR215W)|FD-Score:5.12|P-value:1.57E-7||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GDH2(YDL215C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GPR1(YDL035C)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GTO1(YGR154C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HRQ1(YDR291W_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IMP2'(YIL154C)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP1(YMR204C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC10(YOL015W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTI11(YBL071W-A)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MGR3(YMR115W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MSH1(YHR120W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MVP1(YMR004W)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NOT5(YPR072W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NSI1(YDR026C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:PEX19(YDL065C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO84(YML123C)|FD-Score:6.85|P-value:3.73E-12||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PLB2(YMR006C)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PPN1(YDR452W)|FD-Score:-5.62|P-value:9.29E-9||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:RCK1(YGL158W)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RGD1(YBR260C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:ROM2(YLR371W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL37B(YDR500C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7A(YOR096W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SEC22(YLR268W)|FD-Score:-5.55|P-value:1.41E-8||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEC66(YBR171W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIP2(YGL208W)|FD-Score:4.39|P-value:5.69E-6||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SMK1(YPR054W)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:TRP1(YDR007W)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSA1(YML028W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:URA5(YML106W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:WSS1(YHR134W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAL037W(YAL037W_p)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YBR138C(YBR138C_p)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YIP4(YGL198W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL063C(YKL063C_p)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YKR018C(YKR018C_p)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR444C(YLR444C_d)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Putative protein of unknown function Gene:YMR316C-B(YMR316C-B_d)|FD-Score:8.2|P-value:1.16E-16||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YTP1(YNL237W)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZRG17(YNR039C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency Gene:ADE8(YDR408C)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARF1(YDL192W)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:BUD30(YDL151C_d)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBS1(YDL069C)|FD-Score:-4.28|P-value:9.28E-6||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CIR1(YGR207C)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:CLA4(YNL298W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CTS1(YLR286C)|FD-Score:4|P-value:3.18E-5||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:CYS3(YAL012W)|FD-Score:3.9|P-value:4.71E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DHH1(YDL160C)|FD-Score:5.81|P-value:3.04E-9||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DIA1(YMR316W)|FD-Score:4.96|P-value:3.45E-7||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DPL1(YDR294C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:ECM2(YBR065C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ERC1(YHR032W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FUI1(YBL042C)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAS3(YMR215W)|FD-Score:5.12|P-value:1.57E-7||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GDH2(YDL215C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GPR1(YDL035C)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GTO1(YGR154C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HRQ1(YDR291W_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IMP2'(YIL154C)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP1(YMR204C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC10(YOL015W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTI11(YBL071W-A)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MGR3(YMR115W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MSH1(YHR120W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MVP1(YMR004W)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NOT5(YPR072W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NSI1(YDR026C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:PEX19(YDL065C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO84(YML123C)|FD-Score:6.85|P-value:3.73E-12||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PLB2(YMR006C)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PPN1(YDR452W)|FD-Score:-5.62|P-value:9.29E-9||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:RCK1(YGL158W)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RGD1(YBR260C)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:ROM2(YLR371W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL37B(YDR500C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7A(YOR096W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SEC22(YLR268W)|FD-Score:-5.55|P-value:1.41E-8||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEC66(YBR171W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIP2(YGL208W)|FD-Score:4.39|P-value:5.69E-6||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SMK1(YPR054W)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:TRP1(YDR007W)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSA1(YML028W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:URA5(YML106W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:WSS1(YHR134W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAL037W(YAL037W_p)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YBR138C(YBR138C_p)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YIP4(YGL198W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL063C(YKL063C_p)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YKR018C(YKR018C_p)|FD-Score:3.5|P-value:2.28E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR444C(YLR444C_d)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Putative protein of unknown function Gene:YMR316C-B(YMR316C-B_d)|FD-Score:8.2|P-value:1.16E-16||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YTP1(YNL237W)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZRG17(YNR039C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR051C6.484.63E-112.06RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YJL014W4.424.98E-60.39CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR211W4.032.75E-50.02ZPR1Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress
YDR325W4.013.02E-50.20YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YLR317W_d3.816.91E-50.07YLR317W_dDubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
YPL128C3.749.27E-50.11TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YOL038W3.631.44E-40.00PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YHR062C3.621.45E-40.05RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YBL034C3.571.77E-40.16STU1Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
YER022W3.413.22E-40.13SRB4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
YGR216C3.285.20E-40.12GPI1Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
YOR329C3.158.03E-40.04SCD5Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
YER172C3.119.24E-40.02BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
YML046W3.099.86E-40.11PRP39U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
YGR140W2.990.001410.00CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR316C-B_d8.201.16E-16YMR316C-B_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YML123C6.853.73E-12PHO84High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
YDL160C5.813.04E-9DHH1Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
YMR215W5.121.57E-7GAS3Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation
YPR054W4.983.20E-7SMK1Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p
YMR316W4.963.45E-7DIA1Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YBL042C4.817.54E-7FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YPR072W4.691.39E-6NOT5Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
YGL208W4.395.69E-6SIP2One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane
YKR019C4.376.09E-6IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YKL063C_p4.181.47E-5YKL063C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
YDL011C_d4.042.68E-5YDL011C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W
YGR154C4.032.76E-5GTO1Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
YLR286C4.003.18E-5CTS1Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
YGR207C3.983.49E-5CIR1Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response

GO enrichment analysis for SGTC_763
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1054.29E-16SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.117647
0.1041.56E-15SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.109091TRP & mitochondrial translation
0.0985.53E-14SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.119403
0.0961.34E-13SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.0833333
0.0952.43E-13SGTC_7661391-0721 126.0 μMChemDiv (Drug-like library)10209190.0823529
0.0943.99E-13SGTC_415dibenzyl ketone 100.0 μMMiscellaneous75930.0961538
0.0912.42E-12SGTC_9611252-1571 372.0 μMChemDiv (Drug-like library)68134980.0869565
0.0871.66E-11SGTC_1614065-0411 162.1 μMChemDiv (Drug-like library)19062810.0694444
0.0864.05E-11SGTC_18815652484 16.0 μMMiscellaneous22551290.253521TRP & mitochondrial translation
0.0841.27E-10SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.178082
0.0831.92E-10SGTC_7021488-0190 94.0 μMChemDiv (Drug-like library)9493570.114286amide catabolism
0.0806.88E-10SGTC_2717st081029 62.3 μMTimTec (Natural product derivative library)52928460.0886076
0.0773.00E-9SGTC_22967971656 126.5 μMChembridge (Fragment library)6665700.0641026
0.0764.09E-9SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0958904
0.0764.15E-9SGTC_28619024595 19.5 μMChembridge (Drug-like library)164828100.151899

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_20124023501200 μM0.33477460Chembridge (Fragment library)175.183961.36202
SGTC_2484580981812.04 μM0.2794122870548Miscellaneous355.407583.66915
SGTC_10483161-030024.5 μM0.2631582165101ChemDiv (Drug-like library)266.090722.24102
SGTC_2041543-018462.41 μM0.2571432252213ChemDiv (Drug-like library)356.4138834.76515TRP & mitochondrial translation
SGTC_23369001819198.65 μM0.2545452989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_1881565248416 μM0.2535212255129Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_1921574013650 μM0.2463771774243Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_2001402350257.66 μM0.245283108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_21405586273200 μM0.240741854421Chembridge (Fragment library)223.65562.1402RSC complex & mRNA processing
SGTC_295k015-002731 μM0.2343753887424ChemDiv (Drug-like library)296.277482.8404PDR1