0109-0199

4-methyl-2-phenyl-1H-benzimidazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_780
Screen concentration 192.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 610414
SMILES CC1=C2C(=CC=C1)NC(=N2)C3=CC=CC=C3
Standardized SMILES Cc1cccc2[nH]c(nc12)c3ccccc3
Molecular weight 208.2585
ALogP 3.76
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.33
% growth inhibition (Hom. pool) 9.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 610414
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:-3.16|P-value:7.82E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:-4.66|P-value:1.56E-6|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:ECO1(YFR027W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.02||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:FHL1(YPR104C)|FD-Score:-3.27|P-value:5.46E-4|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:KAE1(YKR038C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.2||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:LAS17(YOR181W)|FD-Score:6.94|P-value:2.02E-12|Clearance:2.08||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MOT1(YPL082C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NOG1(YPL093W)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.07||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NUF2(YOL069W)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.01||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:POP1(YNL221C)|FD-Score:4.85|P-value:6.12E-7|Clearance:0.54||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PTI1(YGR156W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.17||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPT4(YOR259C)|FD-Score:-4.66|P-value:1.56E-6|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RVB2(YPL235W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.04||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC7(YDR170C)|FD-Score:4.31|P-value:8.12E-6|Clearance:0.49||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SGV1(YPR161C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.11||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:TFC3(YAL001C)|FD-Score:-5.34|P-value:4.55E-8|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TFC8(YPL007C)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.02||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:THI80(YOR143C)|FD-Score:-4.92|P-value:4.25E-7|Clearance:0||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:YKL036C(YKL036C_d)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YKL153W(YKL153W_d)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPL238C(YPL238C_d)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:APC5(YOR249C)|FD-Score:-3.16|P-value:7.82E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:-4.66|P-value:1.56E-6|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:ECO1(YFR027W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.02||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:FHL1(YPR104C)|FD-Score:-3.27|P-value:5.46E-4|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:KAE1(YKR038C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.2||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:LAS17(YOR181W)|FD-Score:6.94|P-value:2.02E-12|Clearance:2.08||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MOT1(YPL082C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NOG1(YPL093W)|FD-Score:3.42|P-value:3.13E-4|Clearance:0.07||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NUF2(YOL069W)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.01||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:POP1(YNL221C)|FD-Score:4.85|P-value:6.12E-7|Clearance:0.54||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PTI1(YGR156W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.17||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPT4(YOR259C)|FD-Score:-4.66|P-value:1.56E-6|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RVB2(YPL235W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.04||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC7(YDR170C)|FD-Score:4.31|P-value:8.12E-6|Clearance:0.49||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SGV1(YPR161C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.11||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:TFC3(YAL001C)|FD-Score:-5.34|P-value:4.55E-8|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TFC8(YPL007C)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.02||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:THI80(YOR143C)|FD-Score:-4.92|P-value:4.25E-7|Clearance:0||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:YKL036C(YKL036C_d)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YKL153W(YKL153W_d)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPL238C(YPL238C_d)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 610414
Download HOP data (tab-delimited text)  (excel)
Gene:ADI1(YMR009W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:ATG8(YBL078C)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCH1(YMR237W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BOI1(YBL085W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:CAJ1(YER048C)|FD-Score:7.35|P-value:1.00E-13||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CDH1(YGL003C)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CLB4(YLR210W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COG6(YNL041C)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYC1(YJR048W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAA4(YMR246W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FCY1(YPR062W)|FD-Score:-5.55|P-value:1.41E-8||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:GIR2(YDR152W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:HAC1(YFL031W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HIR1(YBL008W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HOS4(YIL112W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:ICY1(YMR195W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IRC15(YPL017C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LAP2(YNL045W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LRS4(YDR439W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MET14(YKL001C)|FD-Score:3.73|P-value:9.44E-5||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MGT1(YDL200C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:NGL2(YMR285C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NPR1(YNL183C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NVJ2(YPR091C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments Gene:PBS2(YJL128C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PEX3(YDR329C)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PEX31(YGR004W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGU1(YJR153W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins Gene:PMP1(YCR024C-A)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PSP2(YML017W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:QRI5(YLR204W)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD34(YDR314C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:REV7(YIL139C)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RPL22A(YLR061W)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RTC4(YNL254C)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:RUP1(YOR138C)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SCD6(YPR129W)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SCP160(YJL080C)|FD-Score:4.41|P-value:5.25E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCS7(YMR272C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEH1(YGL100W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFB2(YNL049C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SGF11(YPL047W)|FD-Score:5.74|P-value:4.64E-9||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SKT5(YBL061C)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:VHR2(YER064C_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:WSC3(YOL105C)|FD-Score:-4.83|P-value:6.80E-7||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis Gene:YBR051W(YBR051W_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YCR090C(YCR090C_p)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR132C(YDR132C_p)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR250C(YDR250C_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YGL176C(YGL176C_p)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHI9(YHR029C)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHL017W(YHL017W_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YHR180W(YHR180W_d)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL163C(YIL163C_p)|FD-Score:4.96|P-value:3.48E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL027W(YKL027W)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YML082W(YML082W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR160W(YMR160W_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNR065C(YNR065C_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOL099C(YOL099C_d)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YOL118C(YOL118C_d)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP2(YDR326C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ADI1(YMR009W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions Gene:ATG8(YBL078C)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCH1(YMR237W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BOI1(YBL085W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:CAJ1(YER048C)|FD-Score:7.35|P-value:1.00E-13||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CDH1(YGL003C)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CLB4(YLR210W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COG6(YNL041C)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYC1(YJR048W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAA4(YMR246W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FCY1(YPR062W)|FD-Score:-5.55|P-value:1.41E-8||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:GIR2(YDR152W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:HAC1(YFL031W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HIR1(YBL008W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HOS4(YIL112W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:ICY1(YMR195W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IRC15(YPL017C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LAP2(YNL045W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LRS4(YDR439W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MET14(YKL001C)|FD-Score:3.73|P-value:9.44E-5||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MGT1(YDL200C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:NGL2(YMR285C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NPR1(YNL183C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NVJ2(YPR091C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments Gene:PBS2(YJL128C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PEX3(YDR329C)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PEX31(YGR004W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGU1(YJR153W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins Gene:PMP1(YCR024C-A)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PSP2(YML017W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:QRI5(YLR204W)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD34(YDR314C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:REV7(YIL139C)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RPL22A(YLR061W)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RTC4(YNL254C)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:RUP1(YOR138C)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SCD6(YPR129W)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SCP160(YJL080C)|FD-Score:4.41|P-value:5.25E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCS7(YMR272C)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEH1(YGL100W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFB2(YNL049C)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SGF11(YPL047W)|FD-Score:5.74|P-value:4.64E-9||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SKT5(YBL061C)|FD-Score:-3.1|P-value:9.63E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:VHR2(YER064C_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:WSC3(YOL105C)|FD-Score:-4.83|P-value:6.80E-7||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis Gene:YBR051W(YBR051W_d)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YCR090C(YCR090C_p)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR132C(YDR132C_p)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR250C(YDR250C_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YGL176C(YGL176C_p)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YHI9(YHR029C)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHL017W(YHL017W_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YHR180W(YHR180W_d)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR182W(YHR182W_p)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL163C(YIL163C_p)|FD-Score:4.96|P-value:3.48E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL027W(YKL027W)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YML082W(YML082W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR160W(YMR160W_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNR065C(YNR065C_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOL099C(YOL099C_d)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YOL118C(YOL118C_d)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP2(YDR326C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR181W6.942.02E-122.08LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YNL221C4.856.12E-70.54POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YDR170C4.318.12E-60.48SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
YGR156W3.836.51E-50.17PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YPL235W3.661.28E-40.04RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YKR038C3.621.47E-40.20KAE1Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
YPL093W3.423.13E-40.07NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YFR027W3.354.09E-40.02ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YPL007C3.324.42E-40.02TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YOL069W3.314.74E-40.01NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YPR161C3.304.85E-40.11SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YKL153W_d3.197.09E-40.08YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YPL238C_d3.119.27E-40.08YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YAL038W3.030.001210.01CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YJL035C3.030.001240.04TAD2Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER048C7.351.00E-13CAJ1Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
YPL047W5.744.64E-9SGF11Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
YIL163C_p4.963.48E-7YIL163C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YKL027W4.691.37E-6YKL027WProtein of unknown function, localized to the mitochondrial outer membrane
YBL078C4.661.60E-6ATG8Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
YHR159W_p4.562.54E-6TDA11_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
YDR439W4.424.90E-6LRS4Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
YJL080C4.415.25E-6SCP160Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
YMR272C4.131.85E-5SCS7Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
YMR195W4.013.03E-5ICY1Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation
YMR009W3.983.41E-5ADI1Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions
YML017W3.973.52E-5PSP2Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
YER064C_p3.963.68E-5VHR2_pNon-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YIL139C3.934.27E-5REV7Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
YPR129W3.855.82E-5SCD6Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress

GO enrichment analysis for SGTC_780
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1126.76E-18SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)166676790.0285714RNA pol III & RNase P/MRP
0.0911.90E-12SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.0925926Golgi
0.0881.05E-11SGTC_7771315-0167 517.0 μMChemDiv (Drug-like library)42217670.169231Golgi
0.0822.27E-10SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.0793651Golgi
0.0822.64E-10SGTC_999SEC7.066 22.7 μMChemDiv (Drug-like library)7405110.101695Golgi
0.0791.48E-9SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.101695PDR1
0.0707.59E-8SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0410959RPP1 & pyrimidine depletion
0.0709.05E-8SGTC_12410364-0022 50.8 μMChemDiv (Drug-like library)45638950.0847458ERG2
0.0681.60E-7SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.148148Golgi
0.0672.83E-7SGTC_220964-0034 14.5 μMChemDiv (Drug-like library)1384020.0652174unfolded protein response
0.0664.20E-7SGTC_30049076122 71.4 μMChembridge (Drug-like library)447825510.111111
0.0647.25E-7SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.111111ERG2
0.0648.20E-7SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.22449ERG2
0.0604.29E-6SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.0735294
0.0604.31E-6SGTC_12161165-0504 9.7 μMChemDiv (Drug-like library)63040040.115385

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8380109-020371.4 μM0.5759422ChemDiv (Drug-like library)208.258483.75611
SGTC_660109-018742.4 μM0.410256759417ChemDiv (Drug-like library)228.676963.93511
SGTC_5382922-083853.3 μM0.355556777039ChemDiv (Drug-like library)259.305223.43222Golgi
SGTC_3303912561840.58 μM0.30188722695467Chembridge (Drug-like library)332.422144.32714
SGTC_2591skatole100 μM0.2972976736Microsource (Natural product library)131.174462.60910
SGTC_20084012253172 μM0.292683603940Chembridge (Fragment library)185.225142.05513
SGTC_6990375-0592111 μM0.291667762578ChemDiv (Drug-like library)251.326283.71822iron homeostasis
SGTC_8390122-0028230 μM0.288889759448ChemDiv (Drug-like library)265.149023.91711RSC complex & mRNA processing
SGTC_1838st05536984.5 μM0.282609676036TimTec (Natural product derivative library)236.265283.62202
SGTC_1516st013856110 μM0.279075281404TimTec (Pure natural product library)182.22122.45511