0848-0141

2-[(3,5-dibromo-6-oxocyclohexa-2,4-dien-1-ylidene)methylamino]-4,5-dimethylthiophene-3-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_783
Screen concentration 92.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 6755076
SMILES CC1=C(SC(=C1C#N)NC=C2C=C(C=C(C2=O)Br)Br)C
Standardized SMILES Cc1sc(N=Cc2cc(Br)cc(Br)c2O)c(C#N)c1C
Molecular weight 414.115
ALogP 3.3
H-bond donor count 1
H-bond acceptor count 4
Response signature RSC & ERG11

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.16
% growth inhibition (Hom. pool) 11.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6755076
Download HIP data (tab-delimited text)  (excel)
Gene:APC11(YDL008W)|FD-Score:3.76|P-value:8.57E-5|Clearance:0.14||SGD DESC:Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity Gene:ARP7(YPR034W)|FD-Score:7.72|P-value:6.02E-15|Clearance:0.85||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:6.4|P-value:7.56E-11|Clearance:0.17||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC25(YLR310C)|FD-Score:4.38|P-value:6.03E-6|Clearance:0.03||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC53(YDL132W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.03||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CHS2(YBR038W)|FD-Score:3.1|P-value:9.64E-4|Clearance:0.02||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DML1(YMR211W)|FD-Score:4.43|P-value:4.80E-6|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:16.7|P-value:3.48E-63|Clearance:9.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS2(YPL231W)|FD-Score:3.87|P-value:5.35E-5|Clearance:0.12||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GLC7(YER133W)|FD-Score:4.43|P-value:4.75E-6|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSH49(YOR319W)|FD-Score:6.86|P-value:3.36E-12|Clearance:0.46||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MCM6(YGL201C)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.1||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MED7(YOL135C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOT1(YPL082C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.06||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MPS2(YGL075C)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.08||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NOG2(YNR053C)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:POL30(YBR088C)|FD-Score:5.65|P-value:8.05E-9|Clearance:0.42||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:POP1(YNL221C)|FD-Score:-3.46|P-value:2.68E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PSF2(YJL072C)|FD-Score:3.14|P-value:8.56E-4|Clearance:0.04||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RKI1(YOR095C)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.01||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RSC58(YLR033W)|FD-Score:18.1|P-value:9.25E-74|Clearance:9.02||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.72|P-value:5.23E-9|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.23|P-value:8.38E-8|Clearance:0.8||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:STH1(YIL126W)|FD-Score:4.34|P-value:6.98E-6|Clearance:0.31||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUB2(YDL084W)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.11||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SUP35(YDR172W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:SWP1(YMR149W)|FD-Score:3.89|P-value:5.08E-5|Clearance:0.01||SGD DESC:Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:TFC3(YAL001C)|FD-Score:4.03|P-value:2.79E-5|Clearance:0.14||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YPL238C(YPL238C_d)|FD-Score:6.24|P-value:2.25E-10|Clearance:0.51||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:APC11(YDL008W)|FD-Score:3.76|P-value:8.57E-5|Clearance:0.14||SGD DESC:Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity Gene:ARP7(YPR034W)|FD-Score:7.72|P-value:6.02E-15|Clearance:0.85||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:6.4|P-value:7.56E-11|Clearance:0.17||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC25(YLR310C)|FD-Score:4.38|P-value:6.03E-6|Clearance:0.03||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC53(YDL132W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.03||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CHS2(YBR038W)|FD-Score:3.1|P-value:9.64E-4|Clearance:0.02||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:DML1(YMR211W)|FD-Score:4.43|P-value:4.80E-6|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:16.7|P-value:3.48E-63|Clearance:9.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS2(YPL231W)|FD-Score:3.87|P-value:5.35E-5|Clearance:0.12||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GLC7(YER133W)|FD-Score:4.43|P-value:4.75E-6|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSH49(YOR319W)|FD-Score:6.86|P-value:3.36E-12|Clearance:0.46||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MCM6(YGL201C)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.1||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MED7(YOL135C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOT1(YPL082C)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.06||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:MPS2(YGL075C)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.08||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NOG2(YNR053C)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:POL30(YBR088C)|FD-Score:5.65|P-value:8.05E-9|Clearance:0.42||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:POP1(YNL221C)|FD-Score:-3.46|P-value:2.68E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PSF2(YJL072C)|FD-Score:3.14|P-value:8.56E-4|Clearance:0.04||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RKI1(YOR095C)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.01||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RSC58(YLR033W)|FD-Score:18.1|P-value:9.25E-74|Clearance:9.02||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.72|P-value:5.23E-9|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.23|P-value:8.38E-8|Clearance:0.8||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:STH1(YIL126W)|FD-Score:4.34|P-value:6.98E-6|Clearance:0.31||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUB2(YDL084W)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.11||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SUP35(YDR172W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:SWP1(YMR149W)|FD-Score:3.89|P-value:5.08E-5|Clearance:0.01||SGD DESC:Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:TFC3(YAL001C)|FD-Score:4.03|P-value:2.79E-5|Clearance:0.14||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YPL238C(YPL238C_d)|FD-Score:6.24|P-value:2.25E-10|Clearance:0.51||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6755076
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE6(YGR061C)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ANB1(YJR047C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:APS3(YJL024C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ASN2(YGR124W)|FD-Score:-4.8|P-value:8.09E-7||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BUD2(YKL092C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:COG6(YNL041C)|FD-Score:4.98|P-value:3.10E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.4|P-value:7.75E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:6.16|P-value:3.64E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERV46(YAL042W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FBP1(YLR377C)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMP52(YER004W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:HBT1(YDL223C)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:IMA5(YJL216C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IME1(YJR094C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:INO1(YJL153C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRS4(YKR019C)|FD-Score:5.79|P-value:3.42E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LIP2(YLR239C)|FD-Score:6.91|P-value:2.46E-12||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MKK1(YOR231W)|FD-Score:6.74|P-value:8.04E-12||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MPP6(YNR024W)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MUD2(YKL074C)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:MUS81(YDR386W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OPI10(YOL032W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:OPI3(YJR073C)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCL8(YPL219W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEX17(YNL214W)|FD-Score:-4.36|P-value:6.49E-6||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PFK1(YGR240C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PLB1(YMR008C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PMR1(YGL167C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PNG1(YPL096W)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPH22(YDL188C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PYC2(YBR218C)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:REV7(YIL139C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFM1(YOR279C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIM21(YNL294C)|FD-Score:-4.28|P-value:9.19E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16B(YDL083C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SNQ2(YDR011W)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPO23(YBR250W)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SPS2(YDR522C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SVL3(YPL032C)|FD-Score:4.87|P-value:5.53E-7||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SYP1(YCR030C)|FD-Score:-5.88|P-value:2.07E-9||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TIR3(YIL011W)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TMA16(YOR252W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TOS3(YGL179C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:TPK1(YJL164C)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP4(YDR354W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRX3(YCR083W)|FD-Score:-3.92|P-value:4.48E-5||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VID22(YLR373C)|FD-Score:4.37|P-value:6.34E-6||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBR013C(YBR013C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR138C(YBR138C_p)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YBR292C(YBR292C_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:-4.5|P-value:3.32E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDR008C(YDR008C_d)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR193W(YDR193W_d)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGL242C(YGL242C_p)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR122W(YGR122W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR153W(YGR153W_p)|FD-Score:-6.95|P-value:1.88E-12||SGD DESC:Putative protein of unknown function Gene:YHK8(YHR048W_p)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YJR011C(YJR011C_p)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YKL070W(YKL070W_p)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR061C(YNR061C_p)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YRR1(YOR162C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ACO1(YLR304C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE6(YGR061C)|FD-Score:-4.03|P-value:2.84E-5||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ANB1(YJR047C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:APS3(YJL024C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ASN2(YGR124W)|FD-Score:-4.8|P-value:8.09E-7||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BUD2(YKL092C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:COG6(YNL041C)|FD-Score:4.98|P-value:3.10E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.4|P-value:7.75E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:6.16|P-value:3.64E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERV46(YAL042W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FBP1(YLR377C)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMP52(YER004W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:HBT1(YDL223C)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:IMA5(YJL216C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IME1(YJR094C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:INO1(YJL153C)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRS4(YKR019C)|FD-Score:5.79|P-value:3.42E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LIP2(YLR239C)|FD-Score:6.91|P-value:2.46E-12||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MKK1(YOR231W)|FD-Score:6.74|P-value:8.04E-12||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MPP6(YNR024W)|FD-Score:-3.15|P-value:8.24E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MUD2(YKL074C)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:MUS81(YDR386W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OPI10(YOL032W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:OPI3(YJR073C)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCL8(YPL219W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEX17(YNL214W)|FD-Score:-4.36|P-value:6.49E-6||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PFK1(YGR240C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PLB1(YMR008C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol Gene:PMR1(YGL167C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PNG1(YPL096W)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPH22(YDL188C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PYC2(YBR218C)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:REV7(YIL139C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFM1(YOR279C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIM21(YNL294C)|FD-Score:-4.28|P-value:9.19E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16B(YDL083C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SNQ2(YDR011W)|FD-Score:-3.94|P-value:4.00E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPO23(YBR250W)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SPS2(YDR522C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SVL3(YPL032C)|FD-Score:4.87|P-value:5.53E-7||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SYP1(YCR030C)|FD-Score:-5.88|P-value:2.07E-9||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TIR3(YIL011W)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TMA16(YOR252W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TOS3(YGL179C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:TPK1(YJL164C)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRP4(YDR354W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRX3(YCR083W)|FD-Score:-3.92|P-value:4.48E-5||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VID22(YLR373C)|FD-Score:4.37|P-value:6.34E-6||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBR013C(YBR013C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR138C(YBR138C_p)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YBR292C(YBR292C_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:-4.5|P-value:3.32E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDR008C(YDR008C_d)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR193W(YDR193W_d)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGL242C(YGL242C_p)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR122W(YGR122W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR153W(YGR153W_p)|FD-Score:-6.95|P-value:1.88E-12||SGD DESC:Putative protein of unknown function Gene:YHK8(YHR048W_p)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YJR011C(YJR011C_p)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YKL070W(YKL070W_p)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR061C(YNR061C_p)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YRR1(YOR162C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W18.109.25E-749.02RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YHR007C16.703.48E-639.02ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YPR034W7.726.02E-150.85ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR319W6.863.36E-120.46HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YMR033W6.407.56E-110.17ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YPL238C_d6.242.25E-100.51YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YFR037C5.725.23E-90.07RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YBR088C5.658.05E-90.42POL30Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
YML127W5.238.38E-80.80RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YER133W4.434.75E-60.00GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YMR211W4.434.80E-60.05DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YLR310C4.386.03E-60.03CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YIL126W4.346.98E-60.32STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YAL001C4.032.79E-50.14TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YMR149W3.895.08E-50.01SWP1Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR239C6.912.46E-12LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YOR231W6.748.04E-12MKK1MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
YGL005C6.407.75E-11COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YML071C6.163.64E-10COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YKR019C5.793.42E-9IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YLR377C5.022.60E-7FBP1Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p
YNL041C4.983.10E-7COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YPL032C4.875.53E-7SVL3Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress
YPL096W4.611.97E-6PNG1Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p
YLR373C4.376.34E-6VID22Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles
YOR162C4.121.91E-5YRR1Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
YER076C_p3.983.43E-5YER076C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
YDL223C3.934.21E-5HBT1Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
YDL020C3.924.48E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YDR386W3.914.64E-5MUS81Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p

GO enrichment analysis for SGTC_783
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4990SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.396552RSC & ERG11
0.3475.36E-167SGTC_590k048-0076 22.4 μMChemDiv (Drug-like library)68328140.392857RSC & ERG11
0.3454.47E-165SGTC_329k048-0121 21.3 μMChemDiv (Drug-like library)68329020.415094RSC & ERG11
0.3331.03E-152SGTC_203k066-0004 30.9 μMChemDiv (Drug-like library)67954120.366667RSC & ERG11
0.3121.32E-133SGTC_7461095-0025 46.6 μMChemDiv (Drug-like library)67492860.509804RSC & ERG11
0.3122.13E-133SGTC_185k048-0037 25.5 μMChemDiv (Drug-like library)68275890.322581RSC & ERG11
0.3102.36E-131SGTC_322k048-0136 19.3 μMChemDiv (Drug-like library)68232820.365079RSC & ERG11
0.3075.00E-129SGTC_3241315-0088 19.6 μMChemDiv (Drug-like library)67874210.448276RSC & ERG11
0.3034.28E-126SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.289855Golgi
0.2995.57E-122SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.197368RSC & ERG11
0.2904.22E-115SGTC_5660958-0068 16.5 μMChemDiv (Drug-like library)68153650.323529RSC & ERG11
0.2843.67E-110SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.038961RSC complex & mRNA processing
0.2711.03E-99SGTC_331k048-0133 24.5 μMChemDiv (Drug-like library)68135820.4RSC & ERG11
0.2694.55E-98SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.415094Golgi
0.2645.30E-95SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.0634921RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7461095-002546.6 μM0.5098046749286ChemDiv (Drug-like library)377.055081.73915RSC & ERG11
SGTC_3241315-008819.6 μM0.4482766787421ChemDiv (Drug-like library)440.152284.60214RSC & ERG11
SGTC_13531498-12068.11 μM0.4313736756808ChemDiv (Drug-like library)371.024062.76223mitochondrial response to ROS
SGTC_329k048-012121.3 μM0.4150946832902ChemDiv (Drug-like library)480.921193.58212RSC & ERG11
SGTC_1162k048-013020.1 μM0.4150946797406ChemDiv (Drug-like library)431.120624.52312Golgi
SGTC_342k048-008823.3 μM0.4137936820570ChemDiv (Drug-like library)413.060743.1224RSC & ERG11
SGTC_331k048-013324.5 μM0.46813582ChemDiv (Drug-like library)356.012721.85413RSC & ERG11
SGTC_643k048-010628.4 μM0.3965526763920ChemDiv (Drug-like library)419.49573.65213RSC & ERG11
SGTC_590k048-007622.4 μM0.3928576832814ChemDiv (Drug-like library)407.4601833.87413RSC & ERG11
SGTC_203k066-000430.89 μM0.3666676795412ChemDiv (Drug-like library)501.165862.52816RSC & ERG11