1575-0012

4-[bis(diethoxyphosphoryl)methylidene]-2,6-ditert-butylcyclohexa-2,5-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_790
Screen concentration 79.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 378741
SMILES CCOP(=O)(C(=C1C=C(C(=O)C(=C1)C(C)(C)C)C(C)(C)C)P(=O)(OCC)OCC)OCC
Standardized SMILES CCOP(=O)(OCC)C(=C1C=C(C(=O)C(=C1)C(C)(C)C)C(C)(C)C)P(=O)(OCC)OCC
Molecular weight 490.507
ALogP 4
H-bond donor count 0
H-bond acceptor count 7
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.88
% growth inhibition (Hom. pool) 11.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 378741
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:3.47|P-value:2.58E-4|Clearance:0.04||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BDP1(YNL039W)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DML1(YMR211W)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.09||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GLN4(YOR168W)|FD-Score:-3.83|P-value:6.42E-5|Clearance:0||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:HEM12(YDR047W)|FD-Score:3.34|P-value:4.16E-4|Clearance:0.15||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MAS1(YLR163C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.03||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MED7(YOL135C)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.07||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NUD1(YOR373W)|FD-Score:-4.68|P-value:1.43E-6|Clearance:0||SGD DESC:Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance Gene:RPF2(YKR081C)|FD-Score:-3.84|P-value:6.19E-5|Clearance:0||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPN6(YDL097C)|FD-Score:4.73|P-value:1.14E-6|Clearance:1.25||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.89|P-value:4.92E-7|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:-3.86|P-value:5.76E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC13(YLR208W)|FD-Score:14.1|P-value:1.13E-45|Clearance:7.13||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:7|P-value:1.27E-12|Clearance:2.03||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN2(YLR105C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:YJL202C(YJL202C_d)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Gene:YPD1(YDL235C)|FD-Score:-4.25|P-value:1.06E-5|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YTH1(YPR107C)|FD-Score:4.98|P-value:3.26E-7|Clearance:0.08||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ASA1(YPR085C)|FD-Score:3.47|P-value:2.58E-4|Clearance:0.04||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BDP1(YNL039W)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DML1(YMR211W)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.09||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GLN4(YOR168W)|FD-Score:-3.83|P-value:6.42E-5|Clearance:0||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:HEM12(YDR047W)|FD-Score:3.34|P-value:4.16E-4|Clearance:0.15||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MAS1(YLR163C)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.03||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MED7(YOL135C)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.07||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NUD1(YOR373W)|FD-Score:-4.68|P-value:1.43E-6|Clearance:0||SGD DESC:Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance Gene:RPF2(YKR081C)|FD-Score:-3.84|P-value:6.19E-5|Clearance:0||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPN6(YDL097C)|FD-Score:4.73|P-value:1.14E-6|Clearance:1.25||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:4.89|P-value:4.92E-7|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:-3.86|P-value:5.76E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC13(YLR208W)|FD-Score:14.1|P-value:1.13E-45|Clearance:7.13||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:7|P-value:1.27E-12|Clearance:2.03||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEN2(YLR105C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:YJL202C(YJL202C_d)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Gene:YPD1(YDL235C)|FD-Score:-4.25|P-value:1.06E-5|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YTH1(YPR107C)|FD-Score:4.98|P-value:3.26E-7|Clearance:0.08||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 378741
Download HOP data (tab-delimited text)  (excel)
Gene:ALF1(YNL148C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANT1(YPR128C)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APL6(YGR261C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARL1(YBR164C)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG27(YJL178C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BTN2(YGR142W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CHO2(YGR157W)|FD-Score:-4.32|P-value:7.63E-6||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:COG7(YGL005C)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPT1(YNL130C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CYB2(YML054C)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:CYC2(YOR037W)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DPP1(YDR284C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ECM10(YEL030W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:FIT2(YOR382W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GAL11(YOL051W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GIP3(YPL137C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GOS1(YHL031C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GUP1(YGL084C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HDA3(YPR179C)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:IES3(YLR052W)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:INM1(YHR046C)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IPK1(YDR315C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRA2(YOL081W)|FD-Score:5.26|P-value:7.37E-8||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU1(YPL135W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:LDH1(YBR204C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LGE1(YPL055C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LRS4(YDR439W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAC1(YMR021C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRT4(YKL009W)|FD-Score:4.37|P-value:6.28E-6||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MUS81(YDR386W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OPI3(YJR073C)|FD-Score:6.71|P-value:9.86E-12||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC11(YDR488C)|FD-Score:5.3|P-value:5.88E-8||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PAL1(YDR348C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAU23(YLR037C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEP7(YDR323C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PKP2(YGL059W)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:POA1(YBR022W)|FD-Score:5.97|P-value:1.16E-9||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PPH22(YDL188C)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PST1(YDR055W)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:QCR6(YFR033C)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAX2(YLR084C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RGL1(YPL066W)|FD-Score:5.32|P-value:5.05E-8||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:ROT2(YBR229C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPN4(YDL020C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC1(YKL212W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:7.22|P-value:2.52E-13||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SKT5(YBL061C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SNF8(YPL002C)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPF1(YEL031W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPR3(YGR059W)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SWI5(YDR146C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TOF1(YNL273W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TPS2(YDR074W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP4(YDR354W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSA2(YDR453C)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:32.3|P-value:1.98E-229||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UTR2(YEL040W)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:VMA7(YGR020C)|FD-Score:5.62|P-value:9.66E-9||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS21(YOR089C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:6.44|P-value:5.86E-11||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS75(YNL246W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAL067W-A(YAL067W-A_p)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBP1(YBR216C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication Gene:YER034W(YER034W_p)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL117W(YGL117W_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YKE2(YLR200W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR278C(YLR278C_p)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-4.38|P-value:5.80E-6||SGD DESC:Putative protein of unknown function Gene:YLR446W(YLR446W_p)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Putative protein of unknown function Gene:YPK2(YMR104C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPQ1(YOL092W_p)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:ZAP1(YJL056C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ALF1(YNL148C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ANT1(YPR128C)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APL6(YGR261C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARL1(YBR164C)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG27(YJL178C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BTN2(YGR142W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CHO2(YGR157W)|FD-Score:-4.32|P-value:7.63E-6||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:COG7(YGL005C)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPT1(YNL130C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CYB2(YML054C)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:CYC2(YOR037W)|FD-Score:3.73|P-value:9.45E-5||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DPP1(YDR284C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:ECM10(YEL030W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:FIT2(YOR382W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GAL11(YOL051W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GIP3(YPL137C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GOS1(YHL031C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GUP1(YGL084C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HDA3(YPR179C)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:IES3(YLR052W)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:INM1(YHR046C)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IPK1(YDR315C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRA2(YOL081W)|FD-Score:5.26|P-value:7.37E-8||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU1(YPL135W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:LDH1(YBR204C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LGE1(YPL055C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LRS4(YDR439W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAC1(YMR021C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRT4(YKL009W)|FD-Score:4.37|P-value:6.28E-6||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MUS81(YDR386W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:OPI3(YJR073C)|FD-Score:6.71|P-value:9.86E-12||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC11(YDR488C)|FD-Score:5.3|P-value:5.88E-8||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PAL1(YDR348C)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAU23(YLR037C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEP7(YDR323C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PKP2(YGL059W)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:POA1(YBR022W)|FD-Score:5.97|P-value:1.16E-9||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PPH22(YDL188C)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PST1(YDR055W)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:QCR6(YFR033C)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAX2(YLR084C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RGL1(YPL066W)|FD-Score:5.32|P-value:5.05E-8||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:ROT2(YBR229C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPN4(YDL020C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC1(YKL212W)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:7.22|P-value:2.52E-13||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SKT5(YBL061C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SNF8(YPL002C)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPF1(YEL031W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPR3(YGR059W)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SWI5(YDR146C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:TOF1(YNL273W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TPS2(YDR074W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP4(YDR354W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSA2(YDR453C)|FD-Score:4.3|P-value:8.66E-6||SGD DESC:Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:32.3|P-value:1.98E-229||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UTR2(YEL040W)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:VMA7(YGR020C)|FD-Score:5.62|P-value:9.66E-9||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS21(YOR089C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:6.44|P-value:5.86E-11||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS75(YNL246W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAL067W-A(YAL067W-A_p)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBP1(YBR216C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication Gene:YER034W(YER034W_p)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL117W(YGL117W_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YKE2(YLR200W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR278C(YLR278C_p)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-4.38|P-value:5.80E-6||SGD DESC:Putative protein of unknown function Gene:YLR446W(YLR446W_p)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Putative protein of unknown function Gene:YPK2(YMR104C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPQ1(YOL092W_p)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:ZAP1(YJL056C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W14.101.13E-457.13SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YDL195W7.001.27E-122.03SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPR107C4.983.26E-70.08YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YHR062C4.894.92E-70.17RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDL097C4.731.14E-61.25RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YPR085C3.472.58E-40.04ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YMR211W3.442.94E-40.09DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YDR047W3.344.16E-40.15HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YLR163C3.197.13E-40.03MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YOL135C3.158.04E-40.07MED7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YOR117W3.090.001010.03RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YJL086C_d3.060.001120.01YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YDL017W3.050.001150.04CDC7DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p
YKR079C3.010.001307.55E-4TRZ1tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
YHR040W3.010.001310.05BCD1Essential protein required for the accumulation of box C/D snoRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A32.301.98E-229TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOR035C7.222.52E-13SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YJR073C6.719.86E-12OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YDR495C6.445.86E-11VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YBR022W5.971.16E-9POA1Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing
YPR128C5.677.13E-9ANT1Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
YGR020C5.629.66E-9VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YPL066W5.325.05E-8RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YDR488C5.305.88E-8PAC11Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8
YOL081W5.267.37E-8IRA2GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
YFR033C5.181.10E-7QCR6Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
YAL067W-A_p4.904.88E-7YAL067W-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YNL148C4.631.82E-6ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YDR323C4.572.42E-6PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YPL002C4.483.72E-6SNF8Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression

GO enrichment analysis for SGTC_790
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5430SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0333333TSC3-RPN4
0.5290SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0821918TSC3-RPN4
0.5100SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0666667TSC3-RPN4
0.4960SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.125TSC3-RPN4
0.4920SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.116667TSC3-RPN4
0.4620SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0666667TSC3-RPN4
0.4533.63E-297SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0714286TSC3-RPN4
0.4508.60E-293SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.101695TSC3-RPN4
0.4495.35E-291SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0769231TSC3-RPN4
0.4245.20E-257SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0877193TSC3-RPN4
0.4243.70E-256SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0555556TSC3-RPN4
0.4197.49E-250SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.115942
0.4026.85E-228SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.15493TSC3-RPN4
0.3935.79E-217SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.0923077TSC3-RPN4
0.3899.88E-212SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.105263

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12220366-00741.18 μM0.196078286520ChemDiv (Drug-like library)245.230741.59204TSC3-RPN4
SGTC_2406ethylparaben405.7 μM0.1956528434Miscellaneous166.17391.79213
SGTC_8221487-0950154 μM0.1904761958375ChemDiv (Drug-like library)379.1755423.39216
SGTC_1871st05744867.3 μM0.189655691747TimTec (Natural product derivative library)297.101462.81204
SGTC_22137173844200 μM0.186441892728Chembridge (Fragment library)259.300382.62204
SGTC_21715739187198.08 μM0.1860472262761Chembridge (Fragment library)222.283520.90324copper-dependent oxidative stress
SGTC_1900st06020072.8 μM0.185185688945TimTec (Natural product derivative library)204.221882.67803
SGTC_1902st06021270.5 μM0.185185688944TimTec (Natural product derivative library)204.221882.42203
SGTC_1904st06022578 μM0.18518566595TimTec (Natural product derivative library)204.221882.67803
SGTC_1907st06021083.7 μM0.185185688793TimTec (Natural product derivative library)204.221882.42203