0488-0545

1-(2,4-dinitrophenyl)pyrrolidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_793
Screen concentration 106.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 292548
SMILES C1CCN(C1)C2=C(C=C(C=C2)[N+](=O)[O-])[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(N2CCCC2)c(c1)[N+](=O)[O-]
Molecular weight 237.212
ALogP 2.24
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.31
% growth inhibition (Hom. pool) 11.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 292548
Download HIP data (tab-delimited text)  (excel)
Gene:BBP1(YPL255W)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.11||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:CDC7(YDL017W)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p Gene:NAF1(YNL124W)|FD-Score:3.83|P-value:6.35E-5|Clearance:0.39||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NIP7(YPL211W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.39||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:PAN1(YIR006C)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PFS2(YNL317W)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.14||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:RER2(YBR002C)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.39||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:-4.57|P-value:2.47E-6|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC37(YKR025W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C37 Gene:TFC6(YDR362C)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.1||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:BBP1(YPL255W)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.11||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:CDC7(YDL017W)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p Gene:NAF1(YNL124W)|FD-Score:3.83|P-value:6.35E-5|Clearance:0.39||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NIP7(YPL211W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.39||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:PAN1(YIR006C)|FD-Score:-3.35|P-value:4.01E-4|Clearance:0||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PFS2(YNL317W)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.14||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:RER2(YBR002C)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.39||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RLP24(YLR009W)|FD-Score:-4.57|P-value:2.47E-6|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPC37(YKR025W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C37 Gene:TFC6(YDR362C)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.1||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 292548
Download HOP data (tab-delimited text)  (excel)
Gene:ADH2(YMR303C)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AHC2(YCR082W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM26(YKL037W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APN1(YKL114C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG3(YJL088W)|FD-Score:5.77|P-value:4.05E-9||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ATG10(YLL042C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BAG7(YOR134W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BAP3(YDR046C)|FD-Score:-3.74|P-value:9.06E-5||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BSC1(YDL037C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BTS1(YPL069C)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD17(YNR027W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CBP1(YJL209W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCP1(YKR066C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CNE1(YAL058W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:COA2(YPL189C-A)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CRG1(YHR209W)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DBF2(YGR092W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:ERI1(YPL096C-A)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:GDH3(YAL062W)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HNT1(YDL125C)|FD-Score:6.3|P-value:1.51E-10||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HSL1(YKL101W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:ISC10(YER180C)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MCH5(YOR306C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MGM101(YJR144W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MYO4(YAL029C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NAM2(YLR382C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OCA1(YNL099C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PCA1(YBR295W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDC5(YLR134W)|FD-Score:-6.54|P-value:3.00E-11||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PDH1(YPR002W)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR18(YNR070W_p)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PHO84(YML123C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PTH1(YHR189W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCN1(YKL159C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REV3(YPL167C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:ROM2(YLR371W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23B(YER117W)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:SCS22(YBL091C-A)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SLF1(YDR515W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SMY1(YKL079W)|FD-Score:4.45|P-value:4.39E-6||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SPG1(YGR236C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPO11(YHL022C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPO19(YPL130W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:SSA4(YER103W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:TFB6(YOR352W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIM21(YGR033C)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TPN1(YGL186C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPS3(YMR261C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP3(YER151C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:WAR1(YML076C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-5.32|P-value:5.15E-8||SGD DESC:Putative protein of unknown function Gene:YBR027C(YBR027C_d)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function Gene:YDL144C(YDL144C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YFR039C(YFR039C_p)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL149W(YGL149W_d)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR126W(YGR126W_p)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR201C(YGR201C_p)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Putative protein of unknown function Gene:YIL029C(YIL029C_p)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL150W(YJL150W_d)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR057C(YMR057C_d)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR014W(YNR014W_p)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YPR147C(YPR147C_p)|FD-Score:-4.1|P-value:2.05E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YSP1(YHR155W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ADH2(YMR303C)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AHC2(YCR082W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM26(YKL037W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APN1(YKL114C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARG3(YJL088W)|FD-Score:5.77|P-value:4.05E-9||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ATG10(YLL042C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BAG7(YOR134W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BAP3(YDR046C)|FD-Score:-3.74|P-value:9.06E-5||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BSC1(YDL037C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BTS1(YPL069C)|FD-Score:5.28|P-value:6.49E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD17(YNR027W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CBP1(YJL209W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CCP1(YKR066C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CNE1(YAL058W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:COA2(YPL189C-A)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CRG1(YHR209W)|FD-Score:5.17|P-value:1.19E-7||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DBF2(YGR092W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:ERI1(YPL096C-A)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:GDH3(YAL062W)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HNT1(YDL125C)|FD-Score:6.3|P-value:1.51E-10||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HSL1(YKL101W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:ISC10(YER180C)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MCH5(YOR306C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MGM101(YJR144W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MYO4(YAL029C)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NAM2(YLR382C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OCA1(YNL099C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PCA1(YBR295W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDC5(YLR134W)|FD-Score:-6.54|P-value:3.00E-11||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PDH1(YPR002W)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR18(YNR070W_p)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PHO84(YML123C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PTH1(YHR189W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RCN1(YKL159C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REV3(YPL167C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:ROM2(YLR371W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23B(YER117W)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:SCS22(YBL091C-A)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SLF1(YDR515W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SMY1(YKL079W)|FD-Score:4.45|P-value:4.39E-6||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SPG1(YGR236C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPO11(YHL022C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SPO19(YPL130W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:SSA4(YER103W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:TFB6(YOR352W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TIM21(YGR033C)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TPN1(YGL186C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPS3(YMR261C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP3(YER151C)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:WAR1(YML076C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-5.32|P-value:5.15E-8||SGD DESC:Putative protein of unknown function Gene:YBR027C(YBR027C_d)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR100C(YCR100C_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function Gene:YDL144C(YDL144C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YFR039C(YFR039C_p)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL149W(YGL149W_d)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR126W(YGR126W_p)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR201C(YGR201C_p)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Putative protein of unknown function Gene:YIL029C(YIL029C_p)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL150W(YJL150W_d)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR057C(YMR057C_d)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR014W(YNR014W_p)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YPR147C(YPR147C_p)|FD-Score:-4.1|P-value:2.05E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YSP1(YHR155W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL211W4.171.54E-50.39NIP7Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
YBR002C4.151.64E-50.39RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YNL124W3.836.35E-50.39NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YDR362C3.442.92E-40.10TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YNL317W3.344.25E-40.14PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YKR025W3.197.06E-40.02RPC37RNA polymerase III subunit C37
YPL255W3.177.53E-40.11BBP1Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p
YHR005C-A3.070.001080.02TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YER126C3.050.001140.13NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YNL118C2.920.001770.03DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
YMR208W2.890.001930.01ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YNR026C2.880.002010.01SEC12Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
YDR407C2.870.002080.02TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YGR156W2.850.002220.00PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YML093W2.840.002256.31E-4UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL125C6.301.51E-10HNT1Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress
YBR027C_d5.891.98E-9YBR027C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER117W5.842.67E-9RPL23BRibosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
YJL088W5.774.05E-9ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YPL069C5.286.49E-8BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YHR209W5.171.19E-7CRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
YGL084C4.592.21E-6GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YNL099C4.552.65E-6OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YMR303C4.552.68E-6ADH2Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1
YKL079W4.454.39E-6SMY1Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins
YCR082W4.151.68E-5AHC2Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex
YGR126W_p4.121.91E-5YGR126W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YGR092W4.111.96E-5DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YER180C3.983.48E-5ISC10Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
YGR150C3.983.48E-5CCM1Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport

GO enrichment analysis for SGTC_793
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0881.42E-11SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.255814
0.0849.86E-11SGTC_1614065-0411 162.1 μMChemDiv (Drug-like library)19062810.047619
0.0831.76E-10SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.08TRP & mitochondrial translation
0.0791.39E-9SGTC_2489cantharidin 73.4 μMICCB bioactive library25450.0638298cell wall signaling
0.0764.50E-9SGTC_140354-0009 295.0 μMChemDiv (Drug-like library)17135070.0833333
0.0756.88E-9SGTC_8580438-0351 2.8 μMChemDiv (Drug-like library)27991190.222222copper-dependent oxidative stress
0.0715.83E-8SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.0638298cell wall signaling
0.0707.48E-8SGTC_1778st046819 58.9 μMTimTec (Natural product derivative library)6717150.184615
0.0681.58E-7SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0483871
0.0663.90E-7SGTC_1892st058411 7.4 μMTimTec (Natural product derivative library)39347380.0793651
0.0655.16E-7SGTC_1548st061468 90.8 μMTimTec (Pure natural product library)3907990.0714286
0.0647.57E-7SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.08TRP & mitochondrial translation
0.0631.48E-6SGTC_13983558-0053 112.0 μMChemDiv (Drug-like library)45323690.1
0.0612.79E-6SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0819672
0.0613.22E-6SGTC_2961222-0039 74.9 μMChemDiv (Drug-like library)59841760.0714286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7680384-003377.3 μM0.3863643965305ChemDiv (Drug-like library)275.216962.43216fatty acid desaturase (OLE1)
SGTC_260302-0163425.16 μM0.3777783100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_300r073-00115.04 μM0.3752735780ChemDiv (Drug-like library)282.683043.03806
SGTC_1893970-07422.12 μM0.3720932891807ChemDiv (Drug-like library)297.06172.03104
SGTC_225nsc-1226571.02 μM0.369565275685Miscellaneous322.29332.72806
SGTC_6260214-000933 μM0.3617023437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_230213-001140 μM0.357143627608ChemDiv (Drug-like library)304.215123.06317copper-dependent oxidative stress
SGTC_1360097-0015404.48 μM0.3571434861337ChemDiv (Drug-like library)494.448642.62701160S ribosome export
SGTC_3710195-00067.36 μM0.353928593ChemDiv (Drug-like library)253.209682.70204
SGTC_244nsc-2078951.3 μM0.34615442640Miscellaneous279.25202-1.27707DNA damage response