k781-0471

4-[(4-chlorophenyl)methylamino]-3-nitrobenzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_797
Screen concentration 270.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 23738129
SMILES C1=CC(=CC=C1CNC2=C(C=C(C=C2)C(=O)O)[N+](=O)[O-])Cl
Standardized SMILES OC(=O)c1ccc(NCc2ccc(Cl)cc2)c(c1)[N+](=O)[O-]
Molecular weight 306.7011
ALogP 3.41
H-bond donor count 2
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.68
% growth inhibition (Hom. pool) 7.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 23738129
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.2||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CSL4(YNL232W)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:GLC7(YER133W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GWT1(YJL091C)|FD-Score:3.22|P-value:6.50E-4|Clearance:0.02||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:LSM5(YER146W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NDC1(YML031W)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.01||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NUT2(YPR168W)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:QRI1(YDL103C)|FD-Score:-3.1|P-value:9.69E-4|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RFA1(YAR007C)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:TIF6(YPR016C)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRM112(YNR046W)|FD-Score:4.43|P-value:4.70E-6|Clearance:0.76||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:UTP22(YGR090W)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.09||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YDR053W(YDR053W_d)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.01||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YRA1(YDR381W)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.14||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:ARC40(YBR234C)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.2||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CSL4(YNL232W)|FD-Score:3.38|P-value:3.62E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:GLC7(YER133W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GWT1(YJL091C)|FD-Score:3.22|P-value:6.50E-4|Clearance:0.02||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:LSM5(YER146W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NDC1(YML031W)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.01||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NUT2(YPR168W)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:QRI1(YDL103C)|FD-Score:-3.1|P-value:9.69E-4|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RFA1(YAR007C)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:TIF6(YPR016C)|FD-Score:-3.25|P-value:5.86E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TRM112(YNR046W)|FD-Score:4.43|P-value:4.70E-6|Clearance:0.76||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:UTP22(YGR090W)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.09||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YDR053W(YDR053W_d)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.01||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YRA1(YDR381W)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.14||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 23738129
Download HOP data (tab-delimited text)  (excel)
Gene:AIM14(YGL160W)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM19(YIL087C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ATC1(YDR184C)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG27(YJL178C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BTS1(YPL069C)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CIT3(YPR001W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CKB2(YOR039W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CSF1(YLR087C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM2(YIL132C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CTK3(YML112W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCS2(YOR173W)|FD-Score:5.01|P-value:2.70E-7||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:ELP4(YPL101W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG4(YGL012W)|FD-Score:12.8|P-value:1.47E-37||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP3(YDL018C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERV2(YPR037C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:EST2(YLR318W)|FD-Score:4.36|P-value:6.48E-6||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAR8(YMR029C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FRE3(YOR381W)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GPA2(YER020W)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPG1(YGL121C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HRB1(YNL004W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HUA1(YGR268C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:ICY2(YPL250C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:ILV1(YER086W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IRC13(YOR235W_d)|FD-Score:6.45|P-value:5.50E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:LAS21(YJL062W)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LRP1(YHR081W)|FD-Score:4|P-value:3.12E-5||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:LSC1(YOR142W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:LYS20(YDL182W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication Gene:MFA1(YDR461W)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MFT1(YML062C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MMT1(YMR177W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MON1(YGL124C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPS12(YNR036C)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MXR1(YER042W)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:OCA5(YHL029C_p)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PDR17(YNL264C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PFK2(YMR205C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMP1(YCR024C-A)|FD-Score:3.72|P-value:9.85E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RCN1(YKL159C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REC114(YMR133W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:RIM9(YMR063W)|FD-Score:5.83|P-value:2.70E-9||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPS23A(YGR118W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SCS7(YMR272C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SHE4(YOR035C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SSA3(YBL075C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SUS1(YBR111W-A)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWC5(YBR231C)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWT1(YOR166C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYS1(YJL004C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:UFD2(YDL190C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:UGA2(YBR006W)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS17(YOR132W)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS72(YDR485C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YDL172C(YDL172C_d)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL218W(YDL218W_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR239C(YDR239C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.83|P-value:6.34E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YET2(YMR040W)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YFL015C(YFL015C_d)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YFL042C(YFL042C_p)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL149W(YGL149W_d)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR021W(YGR021W_p)|FD-Score:-4.59|P-value:2.17E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR050C(YGR050C_p)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR269W(YGR269W_d)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YLF2(YHL014C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YME2(YMR302C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative protein of unknown function Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Putative protein of unknown function Gene:YNR061C(YNR061C_p)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YNR073C(YNR073C_p)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YPL041C(YPL041C_p)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPL080C(YPL080C_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:AIM14(YGL160W)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:AIM19(YIL087C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ATC1(YDR184C)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG27(YJL178C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BTS1(YPL069C)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CIT3(YPR001W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CKB2(YOR039W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CSF1(YLR087C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM2(YIL132C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CTK3(YML112W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCS2(YOR173W)|FD-Score:5.01|P-value:2.70E-7||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:ELP4(YPL101W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG4(YGL012W)|FD-Score:12.8|P-value:1.47E-37||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP3(YDL018C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERV2(YPR037C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:EST2(YLR318W)|FD-Score:4.36|P-value:6.48E-6||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAR8(YMR029C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FRE3(YOR381W)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GPA2(YER020W)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPG1(YGL121C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HRB1(YNL004W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HUA1(YGR268C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:ICY2(YPL250C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:ILV1(YER086W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IRC13(YOR235W_d)|FD-Score:6.45|P-value:5.50E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:LAS21(YJL062W)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LRP1(YHR081W)|FD-Score:4|P-value:3.12E-5||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:LSC1(YOR142W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:LYS20(YDL182W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication Gene:MFA1(YDR461W)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MFT1(YML062C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MMT1(YMR177W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MON1(YGL124C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPS12(YNR036C)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MXR1(YER042W)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:OCA5(YHL029C_p)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PDR17(YNL264C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PFK2(YMR205C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMP1(YCR024C-A)|FD-Score:3.72|P-value:9.85E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RCN1(YKL159C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REC114(YMR133W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:RIM9(YMR063W)|FD-Score:5.83|P-value:2.70E-9||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPS23A(YGR118W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SCS7(YMR272C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SHE4(YOR035C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SSA3(YBL075C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SUS1(YBR111W-A)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWC5(YBR231C)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWT1(YOR166C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYS1(YJL004C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:UFD2(YDL190C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:UGA2(YBR006W)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS17(YOR132W)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS72(YDR485C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YDL172C(YDL172C_d)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL218W(YDL218W_p)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR239C(YDR239C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.83|P-value:6.34E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YET2(YMR040W)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YFL015C(YFL015C_d)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YFL042C(YFL042C_p)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL149W(YGL149W_d)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR021W(YGR021W_p)|FD-Score:-4.59|P-value:2.17E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR050C(YGR050C_p)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR269W(YGR269W_d)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YLF2(YHL014C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YME2(YMR302C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative protein of unknown function Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Putative protein of unknown function Gene:YNR061C(YNR061C_p)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YNR073C(YNR073C_p)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YPL041C(YPL041C_p)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPL080C(YPL080C_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNR046W4.434.70E-60.76TRM112Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
YER146W3.671.20E-40.10LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YBR234C3.581.74E-40.20ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YNL232W3.383.62E-40.02CSL4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
YER133W3.363.90E-40.01GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YGR090W3.354.02E-40.09UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YPR168W3.265.54E-40.02NUT2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
YML031W3.246.01E-40.01NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YDR053W_d3.236.20E-40.01YDR053W_dPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
YJL091C3.226.50E-40.02GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YDR381W3.206.98E-40.14YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YNL251C3.050.001140.01NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YDR168W3.040.001170.12CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YGR065C2.920.001750.01VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YGR002C2.910.001810.05SWC4Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL012W12.801.47E-37ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YOR235W_d6.455.50E-11IRC13_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci
YMR063W5.832.70E-9RIM9Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
YNR036C5.581.20E-8MRPS12Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
YOR173W5.012.70E-7DCS2Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance
YBL075C4.865.94E-7SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YLR087C4.631.86E-6CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGR269W_d4.582.37E-6YGR269W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C
YPL069C4.532.98E-6BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YLR318W4.366.48E-6EST2Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia
YBR231C4.279.81E-6SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YOR166C4.261.01E-5SWT1RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
YMR242W-A_p4.241.12E-5YMR242W-A_pPutative protein of unknown function
YKL159C4.161.60E-5RCN1Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region
YHL029C_p4.141.72E-5OCA5_pCytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts

GO enrichment analysis for SGTC_797
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1353.10E-25SGTC_120rhodamine 6G 52.3 μMChemDiv (Drug-like library)652110.122222mitochondrial processes
0.1315.72E-24SGTC_466geldanamycin 89.2 μMICCB bioactive library52883820.0571429RPP1 & pyrimidine depletion
0.1247.60E-22SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.0615385tubulin folding & SWR complex
0.1241.13E-21SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.0238095Golgi
0.1165.32E-19SGTC_1850st056238 17.0 μMTimTec (Natural product derivative library)53931770.117647RPP1 & pyrimidine depletion
0.1155.82E-19SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.0634921
0.1157.14E-19SGTC_11483909-7663 27.7 μMChemDiv (Drug-like library)40845260.0405405
0.1041.53E-15SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.19697mitochondrial processes
0.1023.19E-15SGTC_3474534-1311 26.2 μMChemDiv (Drug-like library)28620160.217391RPP1 & pyrimidine depletion
0.1025.35E-15SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.0675676
0.1001.31E-14SGTC_23639053361 176.0 μMChembridge (Fragment library)64697970.241379Golgi
0.0984.93E-14SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.0810811tubulin folding & SWR complex
0.0961.63E-13SGTC_550767-0285 6.6 μMChemDiv (Drug-like library)31161660.0740741
0.0952.23E-13SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.0444444calcium & mitochondrial duress
0.0945.59E-13SGTC_2743auranofin 4.3 μMMiscellaneous166676690.0526316RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_30601327-002712.5 μM0.43103416000455ChemDiv (Drug-like library)454.123145.74815
SGTC_30641327-002710 μM0.43103416000455ChemDiv (Drug-like library)454.123145.74815
SGTC_3039909027049.47 μM0.35087717173750Chembridge (Drug-like library)303.740263.07113RPP1 & pyrimidine depletion
SGTC_458nppb133 μM0.3442624549ICCB bioactive library300.309243.52325
SGTC_9252064-127259.6 μM0.343756815817ChemDiv (Drug-like library)333.726481.44825
SGTC_7251497-096319 μM0.3392863549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress
SGTC_1340269-00186.92 μM0.3333334378915ChemDiv (Drug-like library)411.121283.04617
SGTC_7600568-072582.9 μM0.3333333916171ChemDiv (Drug-like library)380.010345.16613
SGTC_1240136-02177.12 μM0.3181823976905ChemDiv (Drug-like library)476.223245.16417mitochondrial stress
SGTC_6880527-0193473 μM0.3114754007154ChemDiv (Drug-like library)286.24293.10827fatty acid desaturase (OLE1)
SGTC_260302-0163425.16 μM0.3035713100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase