1287-0182

4-[5-[(4-bromophenyl)methylideneamino]-1,3-benzoxazol-2-yl]-N,N-dimethylaniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_798
Screen concentration 476.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1649755
SMILES CN(C)C1=CC=C(C=C1)C2=NC3=C(O2)C=CC(=C3)N=CC4=CC=C(C=C4)Br
Standardized SMILES CN(C)c1ccc(cc1)c2oc3ccc(cc3n2)N=Cc4ccc(Br)cc4
Molecular weight 420.3018
ALogP 5.79
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.35
% growth inhibition (Hom. pool) 8.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1649755
Download HIP data (tab-delimited text)  (excel)
Gene:RPN2(YIL075C)|FD-Score:3.8|P-value:7.12E-5|Clearance:0.24||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SFT1(YKL006C-A)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.03||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SPB1(YCL054W)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SWC4(YGR002C)|FD-Score:-3.79|P-value:7.41E-5|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TID3(YIL144W)|FD-Score:3.16|P-value:7.84E-4|Clearance:0.01||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TOP2(YNL088W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.45||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TRS23(YDR246W)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.05||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YJL032W(YJL032W_d)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YOL134C(YOL134C_d)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YTH1(YPR107C)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.15||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:RPN2(YIL075C)|FD-Score:3.8|P-value:7.12E-5|Clearance:0.24||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SFT1(YKL006C-A)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.03||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SPB1(YCL054W)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SWC4(YGR002C)|FD-Score:-3.79|P-value:7.41E-5|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TID3(YIL144W)|FD-Score:3.16|P-value:7.84E-4|Clearance:0.01||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TOP2(YNL088W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.45||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TRS23(YDR246W)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.05||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YJL032W(YJL032W_d)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:YOL134C(YOL134C_d)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YTH1(YPR107C)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.15||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1649755
Download HOP data (tab-delimited text)  (excel)
Gene:ACA1(YER045C)|FD-Score:5.79|P-value:3.47E-9||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ADE17(YMR120C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ADO1(YJR105W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ADP1(YCR011C)|FD-Score:3.83|P-value:6.34E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:APS2(YJR058C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:AQY2(YLL052C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARI1(YGL157W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:COG8(YML071C)|FD-Score:6.22|P-value:2.43E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL7(YIR031C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DBP1(YPL119C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FKS1(YLR342W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GPT2(YKR067W)|FD-Score:-3.79|P-value:7.64E-5||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HNT2(YDR305C)|FD-Score:6.01|P-value:9.08E-10||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:IGO2(YHR132W-A)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:IRS4(YKR019C)|FD-Score:9.35|P-value:4.40E-21||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAL11(YGR289C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MRPL20(YKR085C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MXR1(YER042W)|FD-Score:-3.78|P-value:7.96E-5||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NEJ1(YLR265C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PAU5(YFL020C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PCL10(YGL134W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PIB1(YDR313C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIN3(YPR154W)|FD-Score:4.84|P-value:6.56E-7||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PLM2(YDR501W)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication Gene:PRM9(YAR031W)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:QDR3(YBR043C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:REC114(YMR133W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:REH1(YLR387C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF1(YBR275C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RMD9(YGL107C)|FD-Score:-4.42|P-value:4.99E-6||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:ROD1(YOR018W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:6.29|P-value:1.61E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:SAF1(YBR280C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SEF1(YBL066C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SOL2(YCR073W-A)|FD-Score:4|P-value:3.11E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:5.4|P-value:3.31E-8||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO21(YOL091W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SUR1(YPL057C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:7.14|P-value:4.77E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TCB1(YOR086C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TEF2(YBR118W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP4(YDR354W)|FD-Score:4.32|P-value:7.98E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMR1(YHL035C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS45(YGL095C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS62(YGR141W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:WAR1(YML076C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:WHI2(YOR043W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:YDR008C(YDR008C_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR338C(YDR338C_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YER046W-A(YER046W-A_d)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL132W(YGL132W_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YGL235W(YGL235W_p)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YIL029C(YIL029C_p)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YLR434C(YLR434C_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR057C(YMR057C_d)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR111C(YMR111C_p)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR253C(YMR253C_p)|FD-Score:6.73|P-value:8.53E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL042W-B(YNL042W-B_p)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Putative protein of unknown function Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-5.05|P-value:2.21E-7||SGD DESC:Putative protein of unknown function Gene:YOR097C(YOR097C_p)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR186W(YOR186W_p)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPK2(YMR104C)|FD-Score:4.93|P-value:4.15E-7||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPL229W(YPL229W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR123C(YPR123C_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTA6(YPL074W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ACA1(YER045C)|FD-Score:5.79|P-value:3.47E-9||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ADE17(YMR120C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ADO1(YJR105W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ADP1(YCR011C)|FD-Score:3.83|P-value:6.34E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:APS2(YJR058C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex Gene:AQY2(YLL052C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARI1(YGL157W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:COG8(YML071C)|FD-Score:6.22|P-value:2.43E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL7(YIR031C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DBP1(YPL119C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FKS1(YLR342W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GPT2(YKR067W)|FD-Score:-3.79|P-value:7.64E-5||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HNT2(YDR305C)|FD-Score:6.01|P-value:9.08E-10||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:IGO2(YHR132W-A)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:IRS4(YKR019C)|FD-Score:9.35|P-value:4.40E-21||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAL11(YGR289C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MRPL20(YKR085C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MXR1(YER042W)|FD-Score:-3.78|P-value:7.96E-5||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NEJ1(YLR265C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PAU5(YFL020C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PCL10(YGL134W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PIB1(YDR313C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIN3(YPR154W)|FD-Score:4.84|P-value:6.56E-7||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PLM2(YDR501W)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication Gene:PRM9(YAR031W)|FD-Score:-3.88|P-value:5.17E-5||SGD DESC:Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family Gene:QDR3(YBR043C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:REC114(YMR133W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:REH1(YLR387C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF1(YBR275C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RMD9(YGL107C)|FD-Score:-4.42|P-value:4.99E-6||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:ROD1(YOR018W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:6.29|P-value:1.61E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:SAF1(YBR280C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SEF1(YBL066C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SOL2(YCR073W-A)|FD-Score:4|P-value:3.11E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:5.4|P-value:3.31E-8||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO21(YOL091W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SUR1(YPL057C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:7.14|P-value:4.77E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TCB1(YOR086C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TEF2(YBR118W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP4(YDR354W)|FD-Score:4.32|P-value:7.98E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMR1(YHL035C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS45(YGL095C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS62(YGR141W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:WAR1(YML076C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:WHI2(YOR043W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression Gene:YDR008C(YDR008C_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR338C(YDR338C_p)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YER046W-A(YER046W-A_d)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL132W(YGL132W_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YGL235W(YGL235W_p)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YIL029C(YIL029C_p)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YLR434C(YLR434C_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR057C(YMR057C_d)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR111C(YMR111C_p)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR253C(YMR253C_p)|FD-Score:6.73|P-value:8.53E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL042W-B(YNL042W-B_p)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Putative protein of unknown function Gene:YNL067W-B(YNL067W-B_p)|FD-Score:-5.05|P-value:2.21E-7||SGD DESC:Putative protein of unknown function Gene:YOR097C(YOR097C_p)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR186W(YOR186W_p)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPK2(YMR104C)|FD-Score:4.93|P-value:4.15E-7||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPL229W(YPL229W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR123C(YPR123C_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YTA6(YPL074W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL088W4.251.06E-50.45TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YIL075C3.807.12E-50.24RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YJL032W_d3.561.85E-40.09YJL032W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
YPR107C3.472.56E-40.15YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YCR052W3.324.47E-40.11RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YDR246W3.216.60E-40.05TRS23One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
YIL144W3.167.84E-40.01TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YKL006C-A3.158.14E-40.03SFT1Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
YOL134C_d3.129.14E-40.05YOL134C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
YLL031C3.060.001100.01GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YKL059C3.060.001120.10MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YDR196C2.950.001580.09CAB5Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
YDL141W2.860.002110.02BPL1Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
YKL012W2.840.002260.01PRP40U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
YHR058C2.830.002330.08MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR019C9.354.40E-21IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YJL004C7.144.77E-13SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YMR253C_p6.738.53E-12YMR253C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
YLR287C-A6.291.61E-10RPS30AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
YML071C6.222.43E-10COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR305C6.019.08E-10HNT2Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins
YER045C5.793.47E-9ACA1ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication
YER150W5.403.31E-8SPI1GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p
YMR104C4.934.15E-7YPK2Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
YPR154W4.846.56E-7PIN3Protein that induces appearance of [PIN+] prion when overproduced
YOL018C4.552.74E-6TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YGL235W_p4.424.90E-6YGL235W_pPutative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YNL042W-B_p4.405.44E-6YNL042W-B_pPutative protein of unknown function
YDR354W4.327.98E-6TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YLR434C_d4.241.12E-5YLR434C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W

GO enrichment analysis for SGTC_798
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1561.72E-33SGTC_507nimesulide 162.0 μMMiscellaneous44950.0632911
0.1454.92E-29SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0704225Golgi
0.1402.13E-27SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.430769Golgi
0.1404.36E-27SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.0923077Golgi
0.1396.71E-27SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0641026
0.1366.77E-26SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0833333Golgi
0.1344.09E-25SGTC_1078idebenone 36.1 μMMiscellaneous36860.0273973Golgi
0.1301.01E-23SGTC_2231micatex 6.9 μMMiscellaneous207370.101695
0.1301.55E-23SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.0769231
0.1292.74E-23SGTC_2811672-3440 14.9 μMChemDiv (Drug-like library)68330000.181818Golgi
0.1271.19E-22SGTC_22777947813 106.9 μMChembridge (Fragment library)12467980.0945946RSC complex & mRNA processing
0.1257.21E-22SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0857143
0.1231.88E-21SGTC_7273032-0951 202.0 μMChemDiv (Drug-like library)36528360.075
0.1194.26E-20SGTC_1090132-0036 33.7 μMChemDiv (Drug-like library)672740.0649351
0.1196.23E-20SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.101449

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1100850-0284111.64 μM0.553906265ChemDiv (Drug-like library)391.26066.11103
SGTC_1380850-025940.92 μM0.4477616813004ChemDiv (Drug-like library)407.265.04614cell wall
SGTC_8071315-015148 μM0.4347834011080ChemDiv (Drug-like library)393.39425.6805
SGTC_6051315-0399119 μM0.43076924020461ChemDiv (Drug-like library)381.254666.69203Golgi
SGTC_7771315-0167517 μM0.4242424221767ChemDiv (Drug-like library)381.254666.69203Golgi
SGTC_5741315-039740 μM0.397266794277ChemDiv (Drug-like library)437.285984.17615
SGTC_774k060-0086136 μM0.3934435911235ChemDiv (Drug-like library)280.324443.67815
SGTC_5591550-0661158 μM0.3731345830875ChemDiv (Drug-like library)427.710427.2303
SGTC_7381315-000372.4 μM0.3661976251474ChemDiv (Drug-like library)372.846886.38803
SGTC_6941315-043843.2 μM0.3561646793154ChemDiv (Drug-like library)378.80843.90215