4488-1585

2-[(3-methylbenzoyl)amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_803
Screen concentration 147.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 747204
SMILES CC1=CC=CC(=C1)C(=O)NC2=C(C3=C(S2)CCCC3)C(=O)O
Standardized SMILES Cc1cccc(c1)C(=O)Nc2sc3CCCCc3c2C(=O)O
Molecular weight 315.3868
ALogP 3.9
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.78
% growth inhibition (Hom. pool) 2.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 747204
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:-3.27|P-value:5.43E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCL1(YPR025C)|FD-Score:-3.16|P-value:7.77E-4|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:ERG1(YGR175C)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.37||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:FHL1(YPR104C)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GPI11(YDR302W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:INN1(YNL152W)|FD-Score:-3.12|P-value:9.07E-4|Clearance:0||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:NRD1(YNL251C)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.37||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PRP6(YBR055C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFA1(YAR007C)|FD-Score:-3.64|P-value:1.39E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RIO2(YNL207W)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.01||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPA190(YOR341W)|FD-Score:4.4|P-value:5.46E-6|Clearance:0.37||SGD DESC:RNA polymerase I largest subunit A190 Gene:SPT6(YGR116W)|FD-Score:3.1|P-value:9.58E-4|Clearance:0.2||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SUA5(YGL169W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.34||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI3(YPL237W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.05||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:THI80(YOR143C)|FD-Score:3.8|P-value:7.18E-5|Clearance:0.22||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:TIM10(YHR005C-A)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.04||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOP2(YNL088W)|FD-Score:3.83|P-value:6.50E-5|Clearance:0.02||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YGR265W(YGR265W_d)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:BMS1(YPL217C)|FD-Score:-3.27|P-value:5.43E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCL1(YPR025C)|FD-Score:-3.16|P-value:7.77E-4|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:ERG1(YGR175C)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.37||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:FHL1(YPR104C)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:GPI11(YDR302W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:INN1(YNL152W)|FD-Score:-3.12|P-value:9.07E-4|Clearance:0||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:NRD1(YNL251C)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.37||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PRP6(YBR055C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFA1(YAR007C)|FD-Score:-3.64|P-value:1.39E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RIO2(YNL207W)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.01||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPA190(YOR341W)|FD-Score:4.4|P-value:5.46E-6|Clearance:0.37||SGD DESC:RNA polymerase I largest subunit A190 Gene:SPT6(YGR116W)|FD-Score:3.1|P-value:9.58E-4|Clearance:0.2||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SUA5(YGL169W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.34||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI3(YPL237W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.05||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:THI80(YOR143C)|FD-Score:3.8|P-value:7.18E-5|Clearance:0.22||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:TIM10(YHR005C-A)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.04||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOP2(YNL088W)|FD-Score:3.83|P-value:6.50E-5|Clearance:0.02||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YGR265W(YGR265W_d)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 747204
Download HOP data (tab-delimited text)  (excel)
Gene:ALK2(YBL009W)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ARA2(YMR041C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ARL1(YBR164C)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AST2(YER101C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BSC1(YDL037C)|FD-Score:-4.04|P-value:2.73E-5||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COX12(YLR038C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CRP1(YHR146W)|FD-Score:4.79|P-value:8.42E-7||SGD DESC:Protein that binds to cruciform DNA structures Gene:DNL4(YOR005C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:DPB3(YBR278W)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ELP6(YMR312W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMC4(YGL231C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERP3(YDL018C)|FD-Score:-4.44|P-value:4.44E-6||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GAL2(YLR081W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GIC1(YHR061C)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GPB2(YAL056W)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX2(YDR513W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HAL1(YPR005C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HXT12(YIL170W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IDH2(YOR136W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:KGD1(YIL125W)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LSC2(YGR244C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAM1(YER106W)|FD-Score:4.9|P-value:4.72E-7||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MCM16(YPR046W)|FD-Score:-4.8|P-value:7.76E-7||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MRPS12(YNR036C)|FD-Score:5.74|P-value:4.63E-9||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSA1(YOR066W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:NTC20(YBR188C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PDC5(YLR134W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PIB2(YGL023C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PSY2(YNL201C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RAD24(YER173W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RTN2(YDL204W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTS3(YGR161C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SCD6(YPR129W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SHU1(YHL006C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SSB1(YDL229W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STV1(YMR054W)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TRM10(YOL093W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRM11(YOL124C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:UBC8(YEL012W)|FD-Score:4.76|P-value:9.48E-7||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VID24(YBR105C)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:YAL066W(YAL066W_d)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR250C(YDR250C_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR379C-A(YDR379C-A)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YEL023C(YEL023C_p)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YER038W-A(YER038W-A_d)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER079W(YER079W_p)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Putative protein of unknown function Gene:YFL042C(YFL042C_p)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YHL041W(YHL041W_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YHR035W(YHR035W_p)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YJR124C(YJR124C_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YLF2(YHL014C)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR363W-A(YLR363W-A_p)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YML100W-A(YML100W-A_p)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR265C(YMR265C_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative protein of unknown function Gene:YNR073C(YNR073C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL036W(YOL036W_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOL099C(YOL099C_d)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR092W(YPR092W_d)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ALK2(YBL009W)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ARA2(YMR041C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ARL1(YBR164C)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AST2(YER101C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:BSC1(YDL037C)|FD-Score:-4.04|P-value:2.73E-5||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COX12(YLR038C)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CRP1(YHR146W)|FD-Score:4.79|P-value:8.42E-7||SGD DESC:Protein that binds to cruciform DNA structures Gene:DNL4(YOR005C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:DPB3(YBR278W)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ELP6(YMR312W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMC4(YGL231C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERP3(YDL018C)|FD-Score:-4.44|P-value:4.44E-6||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GAL2(YLR081W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GIC1(YHR061C)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GPB2(YAL056W)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GRX2(YDR513W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HAL1(YPR005C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HXT12(YIL170W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IDH2(YOR136W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:KGD1(YIL125W)|FD-Score:-3.76|P-value:8.62E-5||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LSC2(YGR244C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAM1(YER106W)|FD-Score:4.9|P-value:4.72E-7||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MCM16(YPR046W)|FD-Score:-4.8|P-value:7.76E-7||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MRPS12(YNR036C)|FD-Score:5.74|P-value:4.63E-9||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSA1(YOR066W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:NTC20(YBR188C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PDC5(YLR134W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PIB2(YGL023C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PSY2(YNL201C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RAD24(YER173W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RTN2(YDL204W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTS3(YGR161C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SCD6(YPR129W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SHU1(YHL006C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SSB1(YDL229W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STV1(YMR054W)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TRM10(YOL093W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRM11(YOL124C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:UBC8(YEL012W)|FD-Score:4.76|P-value:9.48E-7||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VID24(YBR105C)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:YAL066W(YAL066W_d)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR250C(YDR250C_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR379C-A(YDR379C-A)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YEL023C(YEL023C_p)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YER038W-A(YER038W-A_d)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER079W(YER079W_p)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Putative protein of unknown function Gene:YFL042C(YFL042C_p)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YHL041W(YHL041W_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YHR035W(YHR035W_p)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YJR124C(YJR124C_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YLF2(YHL014C)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR363W-A(YLR363W-A_p)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YML100W-A(YML100W-A_p)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR265C(YMR265C_p)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Putative protein of unknown function Gene:YNR073C(YNR073C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL036W(YOL036W_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOL099C(YOL099C_d)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR092W(YPR092W_d)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR341W4.405.46E-60.37RPA190RNA polymerase I largest subunit A190
YNL251C4.241.10E-50.37NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YGR175C4.201.36E-50.37ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YNL088W3.836.50E-50.02TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YOR143C3.807.18E-50.22THI80Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
YHR005C-A3.581.70E-40.04TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YPL237W3.541.98E-40.05SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YGR265W_d3.492.41E-40.04YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YGL169W3.452.77E-40.34SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YNL207W3.119.29E-40.01RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YGR116W3.109.58E-40.20SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YPL169C2.910.001820.00MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YPL238C_d2.900.001850.02YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YOR329C2.880.001980.00SCD5Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
YKL082C2.880.001990.09RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNR036C5.744.63E-9MRPS12Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
YER106W4.904.72E-7MAM1Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle
YLR363W-A_p4.856.12E-7YLR363W-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
YHR146W4.798.42E-7CRP1Protein that binds to cruciform DNA structures
YEL012W4.769.48E-7UBC8Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
YER079W_p4.671.47E-6YER079W_pPutative protein of unknown function
YBR278W4.651.68E-6DPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
YER101C4.473.94E-6AST2Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
YMR054W4.434.80E-6STV1Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole
YHR061C4.366.54E-6GIC1Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress
YJR090C3.924.47E-5GRR1F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification
YGL023C3.865.64E-5PIB2Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
YBR164C3.846.05E-5ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YHL014C3.749.15E-5YLF2Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOL036W_p3.729.86E-5YOL036W_pProtein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication

GO enrichment analysis for SGTC_803
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0921.45E-12SGTC_797k781-0471 270.0 μMChemDiv (Drug-like library)237381290.166667RPP1 & pyrimidine depletion
0.0815.18E-10SGTC_1805st050013 60.3 μMTimTec (Natural product derivative library)40371440.116883
0.0772.59E-9SGTC_14294049-0205 9.5 μMChemDiv (Drug-like library)227744780.115942
0.0673.08E-7SGTC_22987976326 193.8 μMChembridge (Fragment library)22358180.102941
0.0655.64E-7SGTC_1705st027868 56.3 μMTimTec (Natural product derivative library)2929480.0746269
0.0596.60E-6SGTC_1901st060216 58.6 μMTimTec (Natural product derivative library)6887920.138462
0.0552.42E-5SGTC_10044084-0029 199.0 μMChemDiv (Drug-like library)7168940.169014
0.0543.56E-5SGTC_2595hexamethylquercetagetin 100.0 μMMicrosource (Natural product library)3863310.0779221
0.0527.08E-5SGTC_9453381-0768 11.3 μMChemDiv (Drug-like library)11262360.0833333endomembrane recycling
0.0511.02E-4SGTC_12780874-0585 172.0 μMChemDiv (Drug-like library)7663060.142857
0.0501.12E-4SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.129032DNA intercalators
0.0491.53E-4SGTC_546peri-Naphthalide 147.0 μMChemDiv (Drug-like library)2238240.111111DNA damage response
0.0491.89E-4SGTC_1950st077106 7.2 μMTimTec (Natural product derivative library)138318640.0978261RNA processing & uracil transport
0.0482.03E-4SGTC_1535tetrahydropapaveroline hydrobromide 54.3 μMTimTec (Pure natural product library)27242780.0985916RPP1 & pyrimidine depletion
0.0482.14E-4SGTC_29469065502 71.4 μMChembridge (Drug-like library)164891010.179487

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_10134112-3890241 μM0.75705171ChemDiv (Drug-like library)331.386183.39725TSC3-RPN4
SGTC_9133487-1245167 μM0.5818184016153ChemDiv (Drug-like library)349.831844.53424
SGTC_2833900131545.46 μM0.4444442989378Chembridge (Drug-like library)358.411522.55425amide catabolism
SGTC_8143342-056996 μM0.43754346283ChemDiv (Drug-like library)305.34893.06125
SGTC_21826325192200 μM0.415094736248Chembridge (Fragment library)226.273762.35422Golgi
SGTC_21935925765200 μM0.410714877370Chembridge (Fragment library)241.28512.49622
SGTC_20134024628200 μM0.4074075230760Chembridge (Fragment library)226.273762.35422
SGTC_9413237-0776460 μM0.392857760008ChemDiv (Drug-like library)275.322922.66224
SGTC_2824799840451.95 μM0.392857837851Chembridge (Drug-like library)257.35073.64212
SGTC_1704st02880570.8 μM0.3833332876101TimTec (Natural product derivative library)282.358622.5724