3966-4455

5-[[5-(4-fluorophenyl)furan-2-yl]methylidene]-2-sulfanylidene-1,3-diazinane-4,6-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_806
Screen concentration 5.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 714982
SMILES C1=CC(=CC=C1C2=CC=C(O2)C=C3C(=O)NC(=S)NC3=O)F
Standardized SMILES Fc1ccc(cc1)c2oc(C=C3C(=O)NC(=S)NC3=O)cc2
Molecular weight 316.307
ALogP 3.17
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.95
% growth inhibition (Hom. pool) 4.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 714982
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.12||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ATP16(YDL004W)|FD-Score:-3.26|P-value:5.63E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CAK1(YFL029C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.13||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT7(YJL111W)|FD-Score:4.29|P-value:8.86E-6|Clearance:0.26||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC31(YOR257W)|FD-Score:5.34|P-value:4.73E-8|Clearance:0.45||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC53(YDL132W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.04||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:EFB1(YAL003W)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.04||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:LSM5(YER146W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MED11(YMR112C)|FD-Score:-3.14|P-value:8.31E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:MED8(YBR193C)|FD-Score:3.91|P-value:4.65E-5|Clearance:0.19||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:RIO1(YOR119C)|FD-Score:-3.24|P-value:6.00E-4|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPB5(YBR154C)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.05||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL15A(YLR029C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RVB2(YPL235W)|FD-Score:-6.34|P-value:1.15E-10|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAR1(YPL218W)|FD-Score:4.89|P-value:5.15E-7|Clearance:0.22||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SCC4(YER147C)|FD-Score:4.03|P-value:2.77E-5|Clearance:0.12||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SEC13(YLR208W)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.37||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SPP41(YDR464W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.06||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SPT5(YML010W)|FD-Score:-4.77|P-value:9.05E-7|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SUI3(YPL237W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.01||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:THG1(YGR024C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.03||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TIF6(YPR016C)|FD-Score:5.37|P-value:3.96E-8|Clearance:0.45||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TOM40(YMR203W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YCG1(YDR325W)|FD-Score:-4.83|P-value:6.92E-7|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YJL009W(YJL009W_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL032W(YJL032W_d)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Gene:ARB1(YER036C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.12||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ATP16(YDL004W)|FD-Score:-3.26|P-value:5.63E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CAK1(YFL029C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.13||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT7(YJL111W)|FD-Score:4.29|P-value:8.86E-6|Clearance:0.26||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC31(YOR257W)|FD-Score:5.34|P-value:4.73E-8|Clearance:0.45||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC53(YDL132W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.04||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:EFB1(YAL003W)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.04||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:LSM5(YER146W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MED11(YMR112C)|FD-Score:-3.14|P-value:8.31E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:MED8(YBR193C)|FD-Score:3.91|P-value:4.65E-5|Clearance:0.19||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:RIO1(YOR119C)|FD-Score:-3.24|P-value:6.00E-4|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPB5(YBR154C)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.05||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL15A(YLR029C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RVB2(YPL235W)|FD-Score:-6.34|P-value:1.15E-10|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAR1(YPL218W)|FD-Score:4.89|P-value:5.15E-7|Clearance:0.22||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SCC4(YER147C)|FD-Score:4.03|P-value:2.77E-5|Clearance:0.12||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SEC13(YLR208W)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.37||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SPP41(YDR464W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.06||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SPT5(YML010W)|FD-Score:-4.77|P-value:9.05E-7|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SUI3(YPL237W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.01||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:THG1(YGR024C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.03||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TIF6(YPR016C)|FD-Score:5.37|P-value:3.96E-8|Clearance:0.45||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TOM40(YMR203W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YCG1(YDR325W)|FD-Score:-4.83|P-value:6.92E-7|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YJL009W(YJL009W_d)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL032W(YJL032W_d)|FD-Score:-3.29|P-value:4.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 714982
Download HOP data (tab-delimited text)  (excel)
Gene:ACS1(YAL054C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AFT1(YGL071W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM24(YJR080C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:APN1(YKL114C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:BNA1(YJR025C)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BSC5(YNR069C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BSP1(YPR171W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CDC26(YFR036W)|FD-Score:7.18|P-value:3.51E-13||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHZ1(YER030W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COQ5(YML110C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:COX10(YPL172C)|FD-Score:4.32|P-value:7.81E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:DAP1(YPL170W)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBF2(YGR092W)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:ELP4(YPL101W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FKH2(YNL068C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FLO8(YER109C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:GEP4(YHR100C)|FD-Score:4.32|P-value:7.75E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLR1(YPL091W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSY2(YLR258W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HGH1(YGR187C)|FD-Score:4.76|P-value:9.74E-7||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:KIN4(YOR233W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LEA1(YPL213W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LGE1(YPL055C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MCM16(YPR046W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDM32(YOR147W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MET1(YKR069W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MGR1(YCL044C)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:NCS2(YNL119W)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PDC5(YLR134W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PET111(YMR257C)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX10(YDR265W)|FD-Score:-4.42|P-value:4.97E-6||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PEX34(YCL056C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHO89(YBR296C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIB1(YDR313C)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PRY2(YKR013W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD30(YDR419W)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RPS30A(YLR287C-A)|FD-Score:7.53|P-value:2.52E-14||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:5.03|P-value:2.50E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTN2(YDL204W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTR2(YDR066C_p)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEY1(YOR165W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SOM1(YEL059C-A)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPO22(YIL073C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STM1(YLR150W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWR1(YDR334W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TCB3(YML072C)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TSC3(YBR058C-A)|FD-Score:10.7|P-value:4.43E-27||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP23(YDR084C)|FD-Score:4.97|P-value:3.36E-7||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMS1(YDR049W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS73(YGL104C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YCR007C(YCR007C_p)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR018C(YDR018C_p)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR537C(YDR537C_d)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YER121W(YER121W_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YER137C(YER137C_p)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:Putative protein of unknown function Gene:YFL015C(YFL015C_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YIL161W(YIL161W_p)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YJR124C(YJR124C_p)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YMR103C(YMR103C_d)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR265C(YMR265C_p)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Putative protein of unknown function Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function Gene:YNL285W(YNL285W_d)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL099C(YOL099C_d)|FD-Score:5.35|P-value:4.34E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPL102C(YPL102C_d)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR063C(YPR063C_p)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:ER-localized protein of unknown function Gene:YSC83(YHR017W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ACS1(YAL054C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AFT1(YGL071W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM24(YJR080C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:APN1(YKL114C)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:BNA1(YJR025C)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BSC5(YNR069C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BSP1(YPR171W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CDC26(YFR036W)|FD-Score:7.18|P-value:3.51E-13||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHZ1(YER030W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COQ5(YML110C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:COX10(YPL172C)|FD-Score:4.32|P-value:7.81E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:DAP1(YPL170W)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBF2(YGR092W)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:ELP4(YPL101W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FKH2(YNL068C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FLO8(YER109C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:GEP4(YHR100C)|FD-Score:4.32|P-value:7.75E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLR1(YPL091W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSY2(YLR258W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HGH1(YGR187C)|FD-Score:4.76|P-value:9.74E-7||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:KIN4(YOR233W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LEA1(YPL213W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LGE1(YPL055C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MCM16(YPR046W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDM32(YOR147W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MET1(YKR069W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MGR1(YCL044C)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:NCS2(YNL119W)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PDC5(YLR134W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PET111(YMR257C)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX10(YDR265W)|FD-Score:-4.42|P-value:4.97E-6||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PEX34(YCL056C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHO89(YBR296C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIB1(YDR313C)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PRY2(YKR013W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD30(YDR419W)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RPS30A(YLR287C-A)|FD-Score:7.53|P-value:2.52E-14||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:5.03|P-value:2.50E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTN2(YDL204W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RTR2(YDR066C_p)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEY1(YOR165W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SOM1(YEL059C-A)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SPO22(YIL073C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:STM1(YLR150W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWR1(YDR334W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TCB3(YML072C)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TSC3(YBR058C-A)|FD-Score:10.7|P-value:4.43E-27||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP23(YDR084C)|FD-Score:4.97|P-value:3.36E-7||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMS1(YDR049W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS73(YGL104C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YCR007C(YCR007C_p)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR018C(YDR018C_p)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR537C(YDR537C_d)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YER121W(YER121W_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YER137C(YER137C_p)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:Putative protein of unknown function Gene:YFL015C(YFL015C_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YIL161W(YIL161W_p)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YJR124C(YJR124C_p)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YMR103C(YMR103C_d)|FD-Score:4.28|P-value:9.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR265C(YMR265C_p)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Putative protein of unknown function Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function Gene:YNL285W(YNL285W_d)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL099C(YOL099C_d)|FD-Score:5.35|P-value:4.34E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPL102C(YPL102C_d)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR063C(YPR063C_p)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:ER-localized protein of unknown function Gene:YSC83(YHR017W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR016C5.373.96E-80.45TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YOR257W5.344.73E-80.45CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YPL218W4.895.15E-70.22SAR1GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport
YLR208W4.661.55E-60.37SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YJL111W4.298.86E-60.26CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YER147C4.032.77E-50.12SCC4Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
YBR193C3.914.65E-50.19MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YPL237W3.711.02E-40.01SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YDL132W3.701.08E-40.04CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YGR024C3.661.27E-40.03THG1tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
YER146W3.621.45E-40.12LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YJL009W_d3.502.29E-40.08YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YDR464W3.433.03E-40.06SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YMR203W3.373.74E-40.03TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YFL029C3.344.15E-40.13CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A10.704.43E-27TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YLR287C-A7.532.52E-14RPS30AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
YFR036W7.183.51E-13CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YER137C_p5.511.82E-8YER137C_pPutative protein of unknown function
YOL099C_d5.354.34E-8YOL099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes
YJR145C5.032.50E-7RPS4AProtein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
YDR084C4.973.36E-7TVP23Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YGR187C4.769.74E-7HGH1Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress
YNL068C4.602.12E-6FKH2Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication
YER030W4.572.44E-6CHZ1Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
YPL213W4.503.44E-6LEA1Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
YJR025C4.395.66E-6BNA13-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YHR100C4.327.75E-6GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YPL172C4.327.81E-6COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YMR103C_d4.289.55E-6YMR103C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_806
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2511.53E-85SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0307692TSC3-RPN4
0.2273.77E-70SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0632911TSC3-RPN4
0.2212.74E-66SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.101695TSC3-RPN4
0.2175.11E-64SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.084507TSC3-RPN4
0.2073.04E-58SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.169492TSC3-RPN4
0.2018.66E-55SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0588235TSC3-RPN4
0.1958.59E-52SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.115385TSC3-RPN4
0.1931.81E-50SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0869565TSC3-RPN4
0.1912.06E-49SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.046875TSC3-RPN4
0.1891.60E-48SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.136364TSC3-RPN4
0.1883.08E-48SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0634921TSC3-RPN4
0.1839.34E-46SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.169492TSC3-RPN4
0.1779.57E-43SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0793651
0.1761.63E-42SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.08TSC3-RPN4
0.1762.90E-42SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.108108

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5883970-114452.9 μM0.4107141888707ChemDiv (Drug-like library)425.2609434.86425
SGTC_2065522159634.26 μM0.382979788581Chembridge (Fragment library)238.286141.71724
SGTC_23067482397179.52 μM0.352941891448Chembridge (Fragment library)282.702882.18634RPP1 & pyrimidine depletion
SGTC_23276189656200 μM0.3529415349088Chembridge (Fragment library)282.702882.18634
SGTC_2305744352269.23 μM0.3272735415886Chembridge (Fragment library)279.2901031.51625
SGTC_12840929-004316 μM0.3061221239883ChemDiv (Drug-like library)223.2235432.14714
SGTC_205252651827.14 μM0.3061221239884Chembridge (Fragment library)223.2235432.14714
SGTC_5564058-006351.5 μM0.2950826615692ChemDiv (Drug-like library)417.276424.07924
SGTC_21365328288199.3 μM0.26262395Chembridge (Fragment library)222.220540.81724
SGTC_22095-(5-methyl-2-thenylidene)-Barbituric acid122.83 μM0.254902616764Chembridge (Fragment library)236.247120.96324