3825-7150

N-(2,4-dimethylphenyl)-2-(3,4,5-trimethoxyphenyl)quinazolin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_810
Screen concentration 143.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 22330411
SMILES CC1=CC(=C(C=C1)NC2=NC(=NC3=CC=CC=C32)C4=CC(=C(C(=C4)OC)OC)OC)C
Standardized SMILES COc1cc(cc(OC)c1OC)c2nc(Nc3ccc(C)cc3C)c4ccccc4n2
Molecular weight 415.4843
ALogP 6.02
H-bond donor count 1
H-bond acceptor count 6
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.06
% growth inhibition (Hom. pool) 9.5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 22330411
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:-3.14|P-value:8.51E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-3.97|P-value:3.65E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:IRA1(YBR140C)|FD-Score:-3.58|P-value:1.73E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:18|P-value:5.49E-73|Clearance:11.7||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:6.34|P-value:1.18E-10|Clearance:2.62||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:PGA1(YNL158W)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.32||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RLP24(YLR009W)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB8(YOR224C)|FD-Score:-4.03|P-value:2.77E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL25(YOL127W)|FD-Score:-3.2|P-value:6.91E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-4.5|P-value:3.42E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS20(YHL015W)|FD-Score:-3.87|P-value:5.34E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.96|P-value:3.71E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:-3.26|P-value:5.56E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RVB1(YDR190C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.27||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC26(YDR238C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.02||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TIF35(YDR429C)|FD-Score:-4.5|P-value:3.38E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRE2(YOR256C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.15||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:-3.73|P-value:9.61E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:CCT3(YJL014W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:-3.14|P-value:8.51E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-3.97|P-value:3.65E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:IRA1(YBR140C)|FD-Score:-3.58|P-value:1.73E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:18|P-value:5.49E-73|Clearance:11.7||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:6.34|P-value:1.18E-10|Clearance:2.62||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:PGA1(YNL158W)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.32||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RLP24(YLR009W)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB8(YOR224C)|FD-Score:-4.03|P-value:2.77E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL25(YOL127W)|FD-Score:-3.2|P-value:6.91E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-4.5|P-value:3.42E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS20(YHL015W)|FD-Score:-3.87|P-value:5.34E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.96|P-value:3.71E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:-3.26|P-value:5.56E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RVB1(YDR190C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.27||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC26(YDR238C)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.02||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TIF35(YDR429C)|FD-Score:-4.5|P-value:3.38E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRE2(YOR256C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.15||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:-3.73|P-value:9.61E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 22330411
Download HOP data (tab-delimited text)  (excel)
Gene:ACF2(YLR144C)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:-5.11|P-value:1.61E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKL1(YBR059C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:BEM4(YPL161C)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUD17(YNR027W)|FD-Score:-6.71|P-value:9.60E-12||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BUD28(YLR062C_d)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBF1(YJR060W)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:DUF1(YOL087C)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM12(YHR021W-A_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity Gene:HST1(YOL068C)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IES3(YLR052W)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:KIN2(YLR096W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KRE28(YDR532C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:LEU3(YLR451W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MAL31(YBR298C)|FD-Score:-3.89|P-value:4.96E-5||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MCM16(YPR046W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MID1(YNL291C)|FD-Score:4.97|P-value:3.37E-7||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:MST1(YKL194C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:OPI3(YJR073C)|FD-Score:-4.87|P-value:5.71E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAU11(YGL261C_p)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDR8(YLR266C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PER1(YCR044C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX19(YDL065C)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO4(YFR034C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:RFM1(YOR279C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL7B(YPL198W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTF1(YGL244W)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SOM1(YEL059C-A)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SSD1(YDR293C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STR3(YGL184C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SUC2(YIL162W)|FD-Score:4.88|P-value:5.41E-7||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUM1(YDR310C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SVP26(YHR181W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:TEF2(YBR118W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP9(YER098W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VMA1(YDL185W)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS70(YJR126C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR100C(YCR100C_p)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Putative protein of unknown function Gene:YDR133C(YDR133C_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YIL102C(YIL102C_p)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Putative protein of unknown function Gene:YJR037W(YJR037W_d)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YJR151W-A(YJR151W-A_p)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKL118W(YKL118W_d)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function Gene:YMR105W-A(YMR105W-A_p)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Putative protein of unknown function Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function Gene:YNR068C(YNR068C_p)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YOR050C(YOR050C_d)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR072W(YOR072W_d)|FD-Score:6.78|P-value:5.94E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL225W(YPL225W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR127W(YPR127W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YTP1(YNL237W)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ACF2(YLR144C)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:-5.11|P-value:1.61E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKL1(YBR059C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:BEM4(YPL161C)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUD17(YNR027W)|FD-Score:-6.71|P-value:9.60E-12||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:BUD28(YLR062C_d)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBF1(YJR060W)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:DUF1(YOL087C)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM12(YHR021W-A_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity Gene:HST1(YOL068C)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IES3(YLR052W)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:KIN2(YLR096W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KRE28(YDR532C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:LEU3(YLR451W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MAL31(YBR298C)|FD-Score:-3.89|P-value:4.96E-5||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MCM16(YPR046W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MID1(YNL291C)|FD-Score:4.97|P-value:3.37E-7||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:MST1(YKL194C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:OPI3(YJR073C)|FD-Score:-4.87|P-value:5.71E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAU11(YGL261C_p)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDR8(YLR266C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PER1(YCR044C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX19(YDL065C)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PHO4(YFR034C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:RFM1(YOR279C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL7B(YPL198W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTF1(YGL244W)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SOM1(YEL059C-A)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:SSD1(YDR293C)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STR3(YGL184C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SUC2(YIL162W)|FD-Score:4.88|P-value:5.41E-7||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUM1(YDR310C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SVP26(YHR181W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:TEF2(YBR118W)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP9(YER098W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VMA1(YDL185W)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS70(YJR126C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR100C(YCR100C_p)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Putative protein of unknown function Gene:YDR133C(YDR133C_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YIL102C(YIL102C_p)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Putative protein of unknown function Gene:YJR037W(YJR037W_d)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YJR151W-A(YJR151W-A_p)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKL118W(YKL118W_d)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR361C-A(YLR361C-A_p)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function Gene:YMR105W-A(YMR105W-A_p)|FD-Score:-3.96|P-value:3.78E-5||SGD DESC:Putative protein of unknown function Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function Gene:YNR068C(YNR068C_p)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YOR050C(YOR050C_d)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR072W(YOR072W_d)|FD-Score:6.78|P-value:5.94E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL225W(YPL225W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR127W(YPR127W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YTP1(YNL237W)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W18.005.49E-7311.70LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W6.341.18E-102.62NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR190C3.721.01E-40.27RVB1ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YNL158W3.452.82E-40.32PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YDR238C3.138.70E-40.02SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YOR256C3.119.21E-40.15TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YIR006C2.960.001530.22PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YHR107C2.740.003050.01CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YNL306W2.730.003140.11MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YNL310C2.630.004320.05ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YDR243C2.570.005020.04PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YNL240C2.530.005700.00NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YHR020W2.530.005730.05YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YDR224C2.480.006580.04HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YAL034C-B_d2.440.007300.00YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR072W_d6.785.94E-12YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YNL291C4.973.37E-7MID1N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
YIL162W4.885.41E-7SUC2Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively
YNR068C_p4.483.68E-6YNR068C_pPutative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form
YGL261C_p4.444.52E-6PAU11_pPutative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2
YJR151W-A_p4.279.97E-6YJR151W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis
YDR310C4.241.12E-5SUM1Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
YPR074C3.924.44E-5TKL1Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
YIL102C_p3.904.74E-5YIL102C_pPutative protein of unknown function
YPL198W3.885.14E-5RPL7BRibosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
YCR100C_p3.875.53E-5YCR100C_pPutative protein of unknown function
YOL068C3.817.07E-5HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YPR127W3.797.50E-5YPR127WPutative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YDR133C_d3.532.04E-4YDR133C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
YHR021W-A_p3.532.05E-4ECM12_pPutative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity

GO enrichment analysis for SGTC_810
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3415.07E-161SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.13513560S ribosome export
0.3287.75E-148SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.16853960S ribosome export
0.3171.40E-137SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.16455760S ribosome export
0.3082.31E-130SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.17567660S ribosome export
0.3053.17E-127SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.090909160S ribosome export
0.2947.81E-118SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.11267660S ribosome export
0.2831.79E-109SGTC_32229128301 49.5 μMChembridge (Drug-like library)49113580.13580260S ribosome export
0.2678.32E-97SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.160S ribosome export
0.2641.67E-94SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.12328860S ribosome export
0.2612.58E-92SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.16455760S ribosome export
0.2541.15E-87SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.1549360S ribosome export
0.2512.95E-85SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.084337360S ribosome export
0.2474.47E-83SGTC_14043909-7820 48.3 μMChemDiv (Drug-like library)237413730.58928660S ribosome export
0.2451.04E-81SGTC_6450845-0943 5.4 μMChemDiv (Drug-like library)18851060.2460S ribosome export
0.2411.59E-78SGTC_14073909-7909 12.3 μMChemDiv (Drug-like library)44117800.35294160S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14043909-782048.3 μM0.58928623741373ChemDiv (Drug-like library)371.431745.551560S ribosome export
SGTC_4253825-701713.1 μM0.453397440ChemDiv (Drug-like library)329.3702435.78914
SGTC_14053909-789412 μM0.4406787104060ChemDiv (Drug-like library)293.362963.7111460S ribosome export
SGTC_14033909-781562.9 μM0.4307696615483ChemDiv (Drug-like library)389.4222035.11216
SGTC_14063909-790611.9 μM0.42623X1406ChemDiv (Drug-like library)307.389543.7734160S ribosome export
SGTC_12900986-024910.2 μM0.38181853329ChemDiv (Drug-like library)300.353844.88613DNA intercalators
SGTC_3287911717337.06 μM0.3770492789709Chembridge (Drug-like library)329.82393.6692460S ribosome export
SGTC_14073909-790912.3 μM0.3529414411780ChemDiv (Drug-like library)319.400244.2011460S ribosome export
SGTC_1983454-287316.19 μM0.35710957ChemDiv (Drug-like library)297.782044.7461360S ribosome export
SGTC_558k064-002721.3 μM0.3442625088526ChemDiv (Drug-like library)334.79895.55113DNA damage response