4251-0442

(NZ)-N-[(Z)-4-(4-ethoxyphenyl)but-3-en-2-ylidene]hydroxylamine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_812
Screen concentration 148.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5723007
SMILES CCOC1=CC=C(C=C1)C=CC(=NO)C
Standardized SMILES CCOc1ccc(C=CC(=NO)C)cc1
Molecular weight 205.253
ALogP 2.43
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.7
% growth inhibition (Hom. pool) 13.35


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5723007
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.17||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CSL4(YNL232W)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.11||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:LCB2(YDR062W)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.06||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MOB1(YIL106W)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:-3.79|P-value:7.51E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POP8(YBL018C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP6(YBR055C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.07||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:QNS1(YHR074W)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.24||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RDS3(YPR094W)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.06||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RRP12(YPL012W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.1||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SEN2(YLR105C)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.11||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:YJL015C(YJL015C_d)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YPI1(YFR003C)|FD-Score:5.05|P-value:2.15E-7|Clearance:1.02||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:ACT1(YFL039C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.17||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CSL4(YNL232W)|FD-Score:4.03|P-value:2.78E-5|Clearance:0.11||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:LCB2(YDR062W)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.06||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MOB1(YIL106W)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:-3.79|P-value:7.51E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POP8(YBL018C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP6(YBR055C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.07||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:QNS1(YHR074W)|FD-Score:3.86|P-value:5.77E-5|Clearance:0.24||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RDS3(YPR094W)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.06||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RRP12(YPL012W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.1||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SEN2(YLR105C)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.11||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:YJL015C(YJL015C_d)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YPI1(YFR003C)|FD-Score:5.05|P-value:2.15E-7|Clearance:1.02||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5723007
Download HOP data (tab-delimited text)  (excel)
Gene:AHC2(YCR082W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:6.31|P-value:1.42E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:AZF1(YOR113W)|FD-Score:4.31|P-value:8.07E-6||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BDH2(YAL061W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:CKB1(YGL019W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CYS3(YAL012W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DMA1(YHR115C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS4(YPR017C)|FD-Score:5.49|P-value:2.00E-8||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM19(YLR390W)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:LEU3(YLR451W)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MSB3(YNL293W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NSG1(YHR133C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OPI3(YJR073C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC10(YGR078C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PKH2(YOL100W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRM5(YIL117C)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTC7(YHR076W)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:SHS1(YDL225W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SNT309(YPR101W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPG1(YGR236C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPO12(YHR152W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SPR3(YGR059W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SSD1(YDR293C)|FD-Score:-3.77|P-value:8.25E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TMA20(YER007C-A)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:5.29|P-value:6.27E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP9(YER098W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VAM7(YGL212W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VMS1(YDR049W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VTH1(YIL173W)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YAP1(YML007W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:5.85|P-value:2.48E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:-5.71|P-value:5.67E-9||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YIL054W(YIL054W_p)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR297W(YLR297W_p)|FD-Score:5.3|P-value:5.64E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-3.79|P-value:7.58E-5||SGD DESC:Putative protein of unknown function Gene:YNR014W(YNR014W_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YOL118C(YOL118C_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AHC2(YCR082W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:6.31|P-value:1.42E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:AZF1(YOR113W)|FD-Score:4.31|P-value:8.07E-6||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BDH2(YAL061W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:CKB1(YGL019W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CYS3(YAL012W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DMA1(YHR115C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS4(YPR017C)|FD-Score:5.49|P-value:2.00E-8||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM19(YLR390W)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:LEU3(YLR451W)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MSB3(YNL293W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NSG1(YHR133C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OPI3(YJR073C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC10(YGR078C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PKH2(YOL100W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRM5(YIL117C)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTC7(YHR076W)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:SHS1(YDL225W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SNT309(YPR101W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPG1(YGR236C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPO12(YHR152W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SPR3(YGR059W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SSD1(YDR293C)|FD-Score:-3.77|P-value:8.25E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TMA20(YER007C-A)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:5.29|P-value:6.27E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP9(YER098W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VAM7(YGL212W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VMS1(YDR049W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VTH1(YIL173W)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YAP1(YML007W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:5.85|P-value:2.48E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:-5.71|P-value:5.67E-9||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YIL054W(YIL054W_p)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR297W(YLR297W_p)|FD-Score:5.3|P-value:5.64E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YMR242W-A(YMR242W-A_p)|FD-Score:-3.79|P-value:7.58E-5||SGD DESC:Putative protein of unknown function Gene:YNR014W(YNR014W_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YOL118C(YOL118C_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR003C5.052.15E-71.02YPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
YNL232W4.032.78E-50.11CSL4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
YDR062W3.924.47E-50.06LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YHR074W3.865.77E-50.24QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YBL018C3.621.48E-40.02POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR105C3.601.59E-40.11SEN2Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
YFL039C3.492.40E-40.17ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YBR055C3.334.41E-40.07PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YPR094W3.255.73E-40.06RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YPL012W3.197.13E-40.10RRP12Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
YJR007W3.090.001000.01SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YPR161C3.080.001030.10SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YDL196W_d2.980.001420.02YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YGR128C2.960.001520.04UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YPR169W2.920.001740.12JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR127W6.311.42E-10ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR008C_d5.852.48E-9YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR017C5.492.00E-8DSS4Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
YLR297W_p5.305.64E-8YLR297W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
YKL211C5.296.27E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIR023W4.671.49E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YOR113W4.318.07E-6AZF1Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity
YGR078C4.181.46E-5PAC10Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
YLR390W3.894.98E-5ECM19Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YIL054W_p3.895.02E-5YIL054W_pProtein of unknown function; expressed at both mRNA and protein levels
YLR451W3.778.14E-5LEU3Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
YML021C3.701.10E-4UNG1Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
YGL212W3.651.30E-4VAM7Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
YGL019W3.522.14E-4CKB1Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
YHR152W3.512.26E-4SPO12Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis

GO enrichment analysis for SGTC_812
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2479.43E-83SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.047619
0.2198.92E-65SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0632911
0.2183.18E-64SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0645161
0.2015.22E-55SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.0483871
0.2002.30E-54SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0909091
0.1982.16E-53SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.218182
0.1982.42E-53SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0517241
0.1923.29E-50SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0615385
0.1911.31E-49SGTC_474dl-dihydrosphingosine 5.0 μMICCB bioactive library30587390.08
0.1899.71E-49SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.149254
0.1892.15E-48SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.037037
0.1862.96E-47SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.203125
0.1831.38E-45SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.208955
0.1825.78E-45SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.0677966
0.1803.93E-44SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.220588

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1737st03555769.7 μM0.55716916TimTec (Natural product derivative library)286.75284.69802
SGTC_6491123-0311367 μM0.404255693629ChemDiv (Drug-like library)241.28513.51613endomembrane recycling
SGTC_9501165-050894.1 μM0.3673476148369ChemDiv (Drug-like library)303.376082.59214
SGTC_1738st03555574 μM0.365331140TimTec (Natural product derivative library)270.2982034.23903TSC3-RPN4
SGTC_26092,3,4-trihydroxy-4'-ethoxybenzophenone70 μM0.3333336708739Microsource (Natural product library)274.268662.8435redox potentiating
SGTC_11730443-0020122 μM0.3265315331295ChemDiv (Drug-like library)254.280563.44313TSC3-RPN4
SGTC_13071187-159912.2 μM0.3191491538471ChemDiv (Drug-like library)296.403365.3512PDR1
SGTC_25272',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone63.64 μM0.3137266728940Microsource (Natural product library)288.295243.06625
SGTC_25772',4'-dihydroxy-4-methoxychalcone15.32 μM0.3137265711223Microsource (Natural product library)270.279963.20124
SGTC_25792',5'-dihydroxy-4-methoxychalcone13.23 μM0.3137265355888Microsource (Natural product library)270.279963.20124