k007-0158

3-chloro-N-[(E)-[phenyl(pyridin-4-yl)methylidene]amino]aniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_813
Screen concentration 173.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6523916
SMILES C1=CC=C(C=C1)C(=NNC2=CC(=CC=C2)Cl)C3=CC=NC=C3
Standardized SMILES Clc1cccc(NN=C(c2ccccc2)c3ccncc3)c1
Molecular weight 307.7769
ALogP 4.61
H-bond donor count 1
H-bond acceptor count 3
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.52
% growth inhibition (Hom. pool) 9.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6523916
Download HIP data (tab-delimited text)  (excel)
Gene:CCA1(YER168C)|FD-Score:3.26|P-value:5.53E-4|Clearance:0.11||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:ERG25(YGR060W)|FD-Score:4.57|P-value:2.46E-6|Clearance:0.48||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:4.85|P-value:6.12E-7|Clearance:0.28||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:FCF2(YLR051C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.04||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:FRS1(YLR060W)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.27||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:FRS2(YFL022C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.04||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:HEM12(YDR047W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.03||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSH49(YOR319W)|FD-Score:5.54|P-value:1.52E-8|Clearance:0.69||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LCD1(YDR499W)|FD-Score:3.29|P-value:5.08E-4|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:NOP9(YJL010C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.01||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PAM18(YLR008C)|FD-Score:6.67|P-value:1.24E-11|Clearance:1.14||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:RRP42(YDL111C)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEC13(YLR208W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SFT1(YKL006C-A)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.08||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SPB4(YFL002C)|FD-Score:-3.93|P-value:4.32E-5|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TOA2(YKL058W)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.02||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:3.81|P-value:6.92E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:CCA1(YER168C)|FD-Score:3.26|P-value:5.53E-4|Clearance:0.11||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:ERG25(YGR060W)|FD-Score:4.57|P-value:2.46E-6|Clearance:0.48||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:4.85|P-value:6.12E-7|Clearance:0.28||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:FCF2(YLR051C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.04||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:FRS1(YLR060W)|FD-Score:4.09|P-value:2.20E-5|Clearance:0.27||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:FRS2(YFL022C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.04||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:HEM12(YDR047W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.03||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSH49(YOR319W)|FD-Score:5.54|P-value:1.52E-8|Clearance:0.69||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LCD1(YDR499W)|FD-Score:3.29|P-value:5.08E-4|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:NOP9(YJL010C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.01||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PAM18(YLR008C)|FD-Score:6.67|P-value:1.24E-11|Clearance:1.14||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:RRP42(YDL111C)|FD-Score:-3.33|P-value:4.30E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEC13(YLR208W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SFT1(YKL006C-A)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.08||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SPB4(YFL002C)|FD-Score:-3.93|P-value:4.32E-5|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TOA2(YKL058W)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.02||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:YDL196W(YDL196W_d)|FD-Score:3.81|P-value:6.92E-5|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6523916
Download HOP data (tab-delimited text)  (excel)
Gene:AHC1(YOR023C)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AIM41(YOR215C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALD2(YMR170C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ARC18(YLR370C)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BCK1(YJL095W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:9.34|P-value:4.73E-21||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:5.65|P-value:8.08E-9||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CSG2(YBR036C)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DIG2(YDR480W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DRS2(YAL026C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM31(YBR176W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ERV14(YGL054C)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FAR3(YMR052W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FET4(YMR319C)|FD-Score:5.64|P-value:8.41E-9||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FZF1(YGL254W)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GCN2(YDR283C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCR2(YNL199C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCS1(YDL226C)|FD-Score:13.6|P-value:1.83E-42||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDA1(YEL042W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GET3(YDL100C)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GPX2(YBR244W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:8.63|P-value:3.16E-18||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRD1(YOL013C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HSP150(YJL159W)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:HUT1(YPL244C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:LRS4(YDR439W)|FD-Score:7.42|P-value:5.70E-14||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MET22(YOL064C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MMR1(YLR190W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MRN1(YPL184C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP49(YKL167C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL28(YDR462W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MVP1(YMR004W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NAB6(YML117W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NEM1(YHR004C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NMD2(YHR077C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NRM1(YNR009W)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NTC20(YBR188C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:NTE1(YML059C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OPI3(YJR073C)|FD-Score:5.84|P-value:2.54E-9||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:4.87|P-value:5.62E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAH1(YMR165C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR16(YNL231C)|FD-Score:4.28|P-value:9.16E-6||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX4(YGR133W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PEX7(YDR142C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PRS4(YBL068W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:8.85|P-value:4.24E-19||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM13(YMR154C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM9(YMR063W)|FD-Score:5.66|P-value:7.62E-9||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RML2(YEL050C)|FD-Score:-5.29|P-value:6.13E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPN4(YDL020C)|FD-Score:8.67|P-value:2.12E-18||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSC1(YGR056W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT103(YDR289C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAS3(YBL052C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SAT4(YCR008W)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SBH2(YER019C-A)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SED4(YCR067C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SLT2(YHR030C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMY1(YKL079W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNF8(YPL002C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPE3(YPR069C)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SSD1(YDR293C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUC2(YIL162W)|FD-Score:4.88|P-value:5.28E-7||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUR4(YLR372W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCB1(YOR086C)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TEA1(YOR337W)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TMA19(YKL056C)|FD-Score:-4.89|P-value:5.01E-7||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRM9(YML014W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:8.06|P-value:3.74E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TUS1(YLR425W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:TVP18(YMR071C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VPS28(YPL065W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:WSC4(YHL028W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YAP1802(YGR241C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBL044W(YBL044W_p)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL083C(YBL083C_d)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YDL172C(YDL172C_d)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YEF1(YEL041W)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YEH2(YLR020C)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YEL010W(YEL010W_d)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function Gene:YER187W(YER187W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YGR139W(YGR139W_d)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:-4.02|P-value:2.94E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL118W(YJL118W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR194C(YLR194C)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YML012C-A(YML012C-A_d)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML054C-A(YML054C-A_p)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:15.8|P-value:2.54E-56||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL261C(YPL261C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPS1(YLR120C)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT31(YER031C)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:AHC1(YOR023C)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AIM41(YOR215C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALD2(YMR170C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ARC18(YLR370C)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BCK1(YJL095W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:9.34|P-value:4.73E-21||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:5.65|P-value:8.08E-9||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CSG2(YBR036C)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DIG2(YDR480W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DRS2(YAL026C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM31(YBR176W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ERV14(YGL054C)|FD-Score:3.75|P-value:8.82E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA1(YOR317W)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FAR3(YMR052W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FET4(YMR319C)|FD-Score:5.64|P-value:8.41E-9||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FZF1(YGL254W)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GCN2(YDR283C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCR2(YNL199C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCS1(YDL226C)|FD-Score:13.6|P-value:1.83E-42||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDA1(YEL042W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GET3(YDL100C)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GPX2(YBR244W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:8.63|P-value:3.16E-18||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRD1(YOL013C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HSP150(YJL159W)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:HUT1(YPL244C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:LRS4(YDR439W)|FD-Score:7.42|P-value:5.70E-14||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MET22(YOL064C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MMR1(YLR190W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MRN1(YPL184C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP49(YKL167C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL28(YDR462W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MVP1(YMR004W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NAB6(YML117W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NEM1(YHR004C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NMD2(YHR077C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:NRM1(YNR009W)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:NTC20(YBR188C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:NTE1(YML059C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OPI3(YJR073C)|FD-Score:5.84|P-value:2.54E-9||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:4.87|P-value:5.62E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAH1(YMR165C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR16(YNL231C)|FD-Score:4.28|P-value:9.16E-6||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX4(YGR133W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PEX7(YDR142C)|FD-Score:-3.21|P-value:6.53E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PRS4(YBL068W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:8.85|P-value:4.24E-19||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM13(YMR154C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM9(YMR063W)|FD-Score:5.66|P-value:7.62E-9||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RML2(YEL050C)|FD-Score:-5.29|P-value:6.13E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPN4(YDL020C)|FD-Score:8.67|P-value:2.12E-18||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSC1(YGR056W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT103(YDR289C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAS3(YBL052C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SAT4(YCR008W)|FD-Score:4.9|P-value:4.89E-7||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SBH2(YER019C-A)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SED4(YCR067C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SLT2(YHR030C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMY1(YKL079W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNF8(YPL002C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPE3(YPR069C)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SSD1(YDR293C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUC2(YIL162W)|FD-Score:4.88|P-value:5.28E-7||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUR4(YLR372W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCB1(YOR086C)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TEA1(YOR337W)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TMA19(YKL056C)|FD-Score:-4.89|P-value:5.01E-7||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRM9(YML014W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:8.06|P-value:3.74E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TUS1(YLR425W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:TVP18(YMR071C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VPS28(YPL065W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:WSC4(YHL028W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YAP1802(YGR241C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBL044W(YBL044W_p)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL083C(YBL083C_d)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YDL172C(YDL172C_d)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YEF1(YEL041W)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YEH2(YLR020C)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YEL010W(YEL010W_d)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function Gene:YER187W(YER187W_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YGR139W(YGR139W_d)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:-4.02|P-value:2.94E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL118W(YJL118W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR194C(YLR194C)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YML012C-A(YML012C-A_d)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML054C-A(YML054C-A_p)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:15.8|P-value:2.54E-56||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL261C(YPL261C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPS1(YLR120C)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT31(YER031C)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR008C6.671.24E-111.14PAM18Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
YOR319W5.541.52E-80.69HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YMR134W_p4.856.12E-70.28ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YGR060W4.572.46E-60.48ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YLR060W4.092.20E-50.27FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YDL196W_d3.816.92E-50.14YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YJL010C3.671.19E-40.01NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YLR051C3.661.26E-40.04FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YFL022C3.621.48E-40.04FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YLR208W3.581.70E-40.21SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YMR290W-A_d3.373.79E-40.05YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YDR047W3.324.57E-40.03HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YDR499W3.295.08E-40.00LCD1Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
YKL058W3.285.15E-40.02TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YER168C3.265.53E-40.11CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W15.802.54E-56YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDL226C13.601.83E-42GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YPL069C9.344.73E-21BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YJL204C8.854.24E-19RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YDL020C8.672.12E-18RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YOR070C8.633.16E-18GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YKL211C8.063.74E-16TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR439W7.425.70E-14LRS4Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
YBR036C6.562.70E-11CSG2Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
YJR073C5.842.54E-9OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YMR063W5.667.62E-9RIM9Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
YCR094W5.658.08E-9CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YMR319C5.648.41E-9FET4Low-affinity Fe(II) transporter of the plasma membrane
YMR154C5.121.53E-7RIM13Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
YER031C4.983.13E-7YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

GO enrichment analysis for SGTC_813
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4052.55E-231SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.166667fatty acid desaturase (OLE1)
0.3891.40E-212SGTC_7680384-0033 77.3 μMChemDiv (Drug-like library)39653050.0634921fatty acid desaturase (OLE1)
0.3887.66E-212SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.145161fatty acid desaturase (OLE1)
0.3454.48E-165SGTC_577r071-0003 38.6 μMChemDiv (Drug-like library)45685170.111111fatty acid desaturase (OLE1)
0.3201.61E-140SGTC_2725haloperidol 53.2 μMMiscellaneous35590.112676plasma membrane duress
0.3141.56E-135SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0422535plasma membrane duress
0.3051.20E-127SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.171053plasma membrane duress
0.3029.44E-125SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.111111plasma membrane duress
0.3021.38E-124SGTC_20365160441 105.0 μMChembridge (Fragment library)1345340.105263NEO1
0.2943.71E-118SGTC_10054092-0690 4.4 μMChemDiv (Drug-like library)15293430.0869565plasma membrane duress
0.2918.01E-116SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.2plasma membrane duress
0.2851.45E-110SGTC_218alverine citrate 93.8 μMMiscellaneous217180.115385fatty acid desaturase (OLE1)
0.2847.52E-110SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.084507endomembrane recycling
0.2834.69E-109SGTC_258haloperidol 50.8 μMMiscellaneous35590.112676fatty acid desaturase (OLE1)
0.2813.11E-107SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.149254fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_208k072-009812.37 μM0.5227275948901ChemDiv (Drug-like library)231.68092.96213heme biosynthesis & mitochondrial translocase
SGTC_384k007-01445.16 μM0.3928576824886ChemDiv (Drug-like library)303.743561.91234ERAD & cell cycle
SGTC_553k007-025645.7 μM0.3541676287000ChemDiv (Drug-like library)229.2528832.48414heme biosynthesis & mitochondrial translocase
SGTC_5840986-024615.4 μM0.35416753315ChemDiv (Drug-like library)304.772925.56712heme requiring
SGTC_352k048-00075.11 μM0.3272736811911ChemDiv (Drug-like library)404.484363.34223ERAD & cell cycle
SGTC_12550566-0045221 μM0.3207552788469ChemDiv (Drug-like library)304.728323.04223excess fatty acid
SGTC_2900532852885.29 μM0.320755795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_563tetrahydrosecaminediol614 μM0.316667334274ChemDiv (Drug-like library)624.898248.24734amide catabolism
SGTC_2787528454071.43 μM0.314815736958Chembridge (Drug-like library)291.729563.24613
SGTC_2892904735858.44 μM0.3103456466771Chembridge (Drug-like library)379.861122.414