1582-0027

N-(3-methoxyphenyl)-4-nitro-2,1,3-benzoxadiazol-7-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_815
Screen concentration 36.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 3128206
SMILES COC1=CC=CC(=C1)NC2=CC=C(C3=NON=C23)[N+](=O)[O-]
Standardized SMILES COc1cccc(Nc2ccc(c3nonc23)[N+](=O)[O-])c1
Molecular weight 286.2429
ALogP 2.83
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.41
% growth inhibition (Hom. pool) 8.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3128206
Download HIP data (tab-delimited text)  (excel)
Gene:CBF5(YLR175W)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.03||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:DPB11(YJL090C)|FD-Score:5.81|P-value:3.18E-9|Clearance:1||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DSN1(YIR010W)|FD-Score:-3.13|P-value:8.62E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FBA1(YKL060C)|FD-Score:-3.98|P-value:3.45E-5|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FCF2(YLR051C)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.01||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:FRS1(YLR060W)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.01||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:IQG1(YPL242C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.03||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAR1(YNL188W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.07||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:KRE33(YNL132W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:PRP5(YBR237W)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RLP7(YNL002C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.13||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPP1(YHR062C)|FD-Score:4.58|P-value:2.29E-6|Clearance:1||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCM3(YDL139C)|FD-Score:3.09|P-value:9.96E-4|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC5(YDR166C)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.06||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SUI2(YJR007W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.05||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFB4(YPR056W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.1||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TRE2(YOR256C)|FD-Score:5.59|P-value:1.12E-8|Clearance:1||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YJL195C(YJL195C_d)|FD-Score:-4.48|P-value:3.77E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YLR458W(YLR458W_d)|FD-Score:4.67|P-value:1.50E-6|Clearance:1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:CBF5(YLR175W)|FD-Score:3.12|P-value:9.14E-4|Clearance:0.03||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:DPB11(YJL090C)|FD-Score:5.81|P-value:3.18E-9|Clearance:1||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DSN1(YIR010W)|FD-Score:-3.13|P-value:8.62E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:FBA1(YKL060C)|FD-Score:-3.98|P-value:3.45E-5|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FCF2(YLR051C)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.01||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:FRS1(YLR060W)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.01||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:IQG1(YPL242C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.03||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAR1(YNL188W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.07||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:KRE33(YNL132W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:PRP5(YBR237W)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RLP7(YNL002C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.13||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPP1(YHR062C)|FD-Score:4.58|P-value:2.29E-6|Clearance:1||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCM3(YDL139C)|FD-Score:3.09|P-value:9.96E-4|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC5(YDR166C)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.06||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SUI2(YJR007W)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.05||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFB4(YPR056W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.1||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TRE2(YOR256C)|FD-Score:5.59|P-value:1.12E-8|Clearance:1||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YJL195C(YJL195C_d)|FD-Score:-4.48|P-value:3.77E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YLR458W(YLR458W_d)|FD-Score:4.67|P-value:1.50E-6|Clearance:1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3128206
Download HOP data (tab-delimited text)  (excel)
Gene:APC9(YLR102C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:APP1(YNL094W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:AQR1(YNL065W)|FD-Score:5.37|P-value:3.92E-8||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ASC1(YMR116C)|FD-Score:3.72|P-value:9.82E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATG29(YPL166W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BLS1(YLR408C_p)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:CAP1(YKL007W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COX6(YHR051W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CPA1(YOR303W)|FD-Score:-5.28|P-value:6.60E-8||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:DAL2(YIR029W)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DCI1(YOR180C)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DDR2(YOL052C-A)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIN7(YDR263C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DPH5(YLR172C)|FD-Score:3.76|P-value:8.41E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ECM30(YLR436C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:EXG2(YDR261C)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FAP1(YNL023C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FCY2(YER056C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FUI1(YBL042C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GMH1(YKR030W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:HGH1(YGR187C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HIS3(YOR202W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:HXT8(YJL214W)|FD-Score:3.78|P-value:7.98E-5||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IBA57(YJR122W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IRC24(YIR036C_p)|FD-Score:3.78|P-value:7.98E-5||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:ISC10(YER180C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ITT1(YML068W)|FD-Score:5.84|P-value:2.63E-9||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:LEU1(YGL009C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:MAC1(YMR021C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDL1(YLR188W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MND2(YIR025W)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRP1(YDR347W)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPS28(YDR337W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC2(YDR205W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSS1(YMR023C)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NAS6(YGR232W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:NRM1(YNR009W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OAZ1(YPL052W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:OPI3(YJR073C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSW1(YOR255W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PCK1(YKR097W)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PCL9(YDL179W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PUF4(YGL014W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:QRI5(YLR204W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAS2(YNL098C)|FD-Score:4.81|P-value:7.53E-7||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RPL2B(YIL018W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL35B(YDL136W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA3(YLR221C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SGM1(YJR134C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SIT1(YEL065W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p Gene:SPI1(YER150W)|FD-Score:5.21|P-value:9.49E-8||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SSK1(YLR006C)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SUM1(YDR310C)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TCB3(YML072C)|FD-Score:4.29|P-value:8.94E-6||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TIR1(YER011W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TSC3(YBR058C-A)|FD-Score:10.2|P-value:8.28E-25||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP18(YMR071C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:TWF1(YGR080W)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly Gene:URH1(YDR400W)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:VBA1(YMR088C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA3(YEL027W)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:YBR074W(YBR074W_p)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Putative metalloprotease Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR064C(YCR064C_d)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL118W(YDL118W_p)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR131C(YDR131C_p)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YDR537C(YDR537C_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YDR541C(YDR541C_p)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER186C(YER186C_p)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative protein of unknown function Gene:YET1(YKL065C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGR137W(YGR137W_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR237C(YGR237C_p)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHL017W(YHL017W_p)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIR020W-A(YIR020W-A_d)|FD-Score:5.58|P-value:1.21E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJR098C(YJR098C_p)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR125W(YLR125W_p)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YNR061C(YNR061C_p)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOR277C(YOR277C_d)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPR027C(YPR027C_p)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function Gene:YPR092W(YPR092W_d)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ZWF1(YNL241C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:APC9(YLR102C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:APP1(YNL094W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:AQR1(YNL065W)|FD-Score:5.37|P-value:3.92E-8||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ASC1(YMR116C)|FD-Score:3.72|P-value:9.82E-5||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATG29(YPL166W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BLS1(YLR408C_p)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:CAP1(YKL007W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COX6(YHR051W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CPA1(YOR303W)|FD-Score:-5.28|P-value:6.60E-8||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:DAL2(YIR029W)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DCI1(YOR180C)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DDR2(YOL052C-A)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIN7(YDR263C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DPH5(YLR172C)|FD-Score:3.76|P-value:8.41E-5||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ECM30(YLR436C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:EXG2(YDR261C)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FAP1(YNL023C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FCY2(YER056C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FUI1(YBL042C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GMH1(YKR030W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:HGH1(YGR187C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HIS3(YOR202W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:HXT8(YJL214W)|FD-Score:3.78|P-value:7.98E-5||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IBA57(YJR122W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IRC24(YIR036C_p)|FD-Score:3.78|P-value:7.98E-5||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:ISC10(YER180C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ITT1(YML068W)|FD-Score:5.84|P-value:2.63E-9||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:LEU1(YGL009C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:MAC1(YMR021C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDL1(YLR188W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MND2(YIR025W)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRP1(YDR347W)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRPS28(YDR337W)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC2(YDR205W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSS1(YMR023C)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NAS6(YGR232W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:NRM1(YNR009W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OAZ1(YPL052W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:OPI3(YJR073C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSW1(YOR255W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PCK1(YKR097W)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PCL9(YDL179W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PUF4(YGL014W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:QRI5(YLR204W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAS2(YNL098C)|FD-Score:4.81|P-value:7.53E-7||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RPL2B(YIL018W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL35B(YDL136W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA3(YLR221C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SGM1(YJR134C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SIT1(YEL065W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p Gene:SPI1(YER150W)|FD-Score:5.21|P-value:9.49E-8||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SSK1(YLR006C)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SUM1(YDR310C)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TCB3(YML072C)|FD-Score:4.29|P-value:8.94E-6||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TIR1(YER011W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TSC3(YBR058C-A)|FD-Score:10.2|P-value:8.28E-25||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP18(YMR071C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:TWF1(YGR080W)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly Gene:URH1(YDR400W)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways Gene:VBA1(YMR088C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA3(YEL027W)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:YBR074W(YBR074W_p)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Putative metalloprotease Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR064C(YCR064C_d)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL118W(YDL118W_p)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR131C(YDR131C_p)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YDR537C(YDR537C_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YDR541C(YDR541C_p)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER186C(YER186C_p)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative protein of unknown function Gene:YET1(YKL065C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGR137W(YGR137W_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR237C(YGR237C_p)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHL017W(YHL017W_p)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIR020W-A(YIR020W-A_d)|FD-Score:5.58|P-value:1.21E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJR098C(YJR098C_p)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR125W(YLR125W_p)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YNR061C(YNR061C_p)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOR277C(YOR277C_d)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPR027C(YPR027C_p)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function Gene:YPR092W(YPR092W_d)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ZWF1(YNL241C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL090C5.813.18E-91.00DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YOR256C5.591.12E-81.00TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YLR458W_d4.671.50E-61.00YLR458W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis
YHR062C4.582.29E-61.00RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YNL132W3.591.68E-43.78E-4KRE33Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YPR056W3.591.68E-40.10TFB4Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
YNL002C3.492.43E-40.13RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YLR060W3.363.95E-40.01FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YPL242C3.344.14E-40.03IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YLR051C3.314.67E-40.01FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YJR007W3.304.85E-40.05SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YDR166C3.245.88E-40.06SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YNL188W3.197.13E-40.07KAR1Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
YLR175W3.129.14E-40.03CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YDL139C3.099.96E-40.00SCM3Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A10.208.28E-25TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML068W5.842.63E-9ITT1Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
YLR204W5.601.06E-8QRI5Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
YIR020W-A_d5.581.21E-8YIR020W-A_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YNL065W5.373.92E-8AQR1Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YER150W5.219.49E-8SPI1GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p
YOR180C5.091.79E-7DCI1Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed
YNL098C4.817.53E-7RAS2GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
YNR061C_p4.671.53E-6YNR061C_pProtein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress
YIR025W4.661.58E-6MND2Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
YDR337W4.631.82E-6MRPS28Mitochondrial ribosomal protein of the small subunit
YDR261C4.572.39E-6EXG2Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YGR080W4.532.89E-6TWF1Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly
YLR408C_p4.483.77E-6BLS1_pPutative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
YDR347W4.464.08E-6MRP1Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein

GO enrichment analysis for SGTC_815
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2252.05E-68SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.123457TSC3-RPN4
0.2248.62E-68SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0277778TSC3-RPN4
0.2211.70E-66SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0857143TSC3-RPN4
0.2014.26E-55SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.161765TSC3-RPN4
0.1923.17E-50SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0909091TSC3-RPN4
0.1885.51E-48SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0704225TSC3-RPN4
0.1887.51E-48SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.171429TSC3-RPN4
0.1771.04E-42SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.0147059TSC3-RPN4
0.1762.19E-42SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0746269TSC3-RPN4
0.1762.84E-42SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0724638TSC3-RPN4
0.1763.89E-42SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.105882TSC3-RPN4
0.1714.10E-40SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.166667TSC3-RPN4
0.1718.08E-40SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.142857TSC3-RPN4
0.1631.59E-36SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.1125
0.1628.48E-36SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.056338TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8201582-006059.9 μM0.5384623848840ChemDiv (Drug-like library)290.661983.50816
SGTC_2842900067519.48 μM0.3965522989072Chembridge (Drug-like library)258.272563.114
SGTC_8991083-0020478 μM0.387097767123ChemDiv (Drug-like library)306.701143.15714
SGTC_9292805-0021398 μM0.3661973098024ChemDiv (Drug-like library)369.39443.07216
SGTC_1764st04450293.5 μM0.368407TimTec (Natural product derivative library)214.223.27413
SGTC_9123474-00172.18 μM0.3548393360550ChemDiv (Drug-like library)312.367983.94307
SGTC_13591582-0056520 nM0.3548393661189ChemDiv (Drug-like library)304.688563.99416
SGTC_2900532852885.29 μM0.322034795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_410478-063282.64 μM0.3214293750424ChemDiv (Drug-like library)229.234642.52724
SGTC_3204911418349.47 μM0.31666717027248Chembridge (Drug-like library)329.2463533.40317