0325-0417

2,3,5,6-tetrabromocyclohexa-2,5-diene-1,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_816
Screen concentration 81.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 68101
SMILES C1(=C(C(=O)C(=C(C1=O)Br)Br)Br)Br
Standardized SMILES BrC1=C(Br)C(=O)C(=C(Br)C1=O)Br
Molecular weight 423.679
ALogP 2.5
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 28.05
% growth inhibition (Hom. pool) 5.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 68101
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:-3.75|P-value:8.74E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BPL1(YDL141W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.07||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:ERG11(YHR007C)|FD-Score:-10.1|P-value:1.83E-24|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSH49(YOR319W)|FD-Score:-5.06|P-value:2.15E-7|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRA1(YBR140C)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:IRR1(YIL026C)|FD-Score:-9.32|P-value:5.73E-21|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NUP57(YGR119C)|FD-Score:3.81|P-value:6.96E-5|Clearance:0.07||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PAM18(YLR008C)|FD-Score:3.74|P-value:9.26E-5|Clearance:0.02||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:PRE4(YFR050C)|FD-Score:3.89|P-value:5.06E-5|Clearance:0.08||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE6(YOL038W)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.27||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP2(YNR011C)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PUP1(YOR157C)|FD-Score:-5.24|P-value:7.99E-8|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFC2(YJR068W)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIB5(YBR256C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPA43(YOR340C)|FD-Score:-8.08|P-value:3.22E-16|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RRN6(YBL014C)|FD-Score:-3.2|P-value:6.84E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SKP1(YDR328C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.03||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SNU56(YDR240C)|FD-Score:4.62|P-value:1.92E-6|Clearance:0.73||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRM1(YGL097W)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.19||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:THG1(YGR024C)|FD-Score:-4.44|P-value:4.58E-6|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:UTP8(YGR128C)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.03||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YKL083W(YKL083W_d)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.04||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR076C(YLR076C_d)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:ARP7(YPR034W)|FD-Score:-3.75|P-value:8.74E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BPL1(YDL141W)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.07||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:ERG11(YHR007C)|FD-Score:-10.1|P-value:1.83E-24|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSH49(YOR319W)|FD-Score:-5.06|P-value:2.15E-7|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRA1(YBR140C)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:IRR1(YIL026C)|FD-Score:-9.32|P-value:5.73E-21|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NUP57(YGR119C)|FD-Score:3.81|P-value:6.96E-5|Clearance:0.07||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PAM18(YLR008C)|FD-Score:3.74|P-value:9.26E-5|Clearance:0.02||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:PRE4(YFR050C)|FD-Score:3.89|P-value:5.06E-5|Clearance:0.08||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE6(YOL038W)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.27||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP2(YNR011C)|FD-Score:-3.17|P-value:7.69E-4|Clearance:0||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PUP1(YOR157C)|FD-Score:-5.24|P-value:7.99E-8|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFC2(YJR068W)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIB5(YBR256C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPA43(YOR340C)|FD-Score:-8.08|P-value:3.22E-16|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RRN6(YBL014C)|FD-Score:-3.2|P-value:6.84E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SKP1(YDR328C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.03||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SNU56(YDR240C)|FD-Score:4.62|P-value:1.92E-6|Clearance:0.73||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRM1(YGL097W)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.19||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:THG1(YGR024C)|FD-Score:-4.44|P-value:4.58E-6|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:UTP8(YGR128C)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.03||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YKL083W(YKL083W_d)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.04||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR076C(YLR076C_d)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 68101
Download HOP data (tab-delimited text)  (excel)
Gene:ALY2(YJL084C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:COQ8(YGL119W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:CPA2(YJR109C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CYB2(YML054C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DIA3(YDL024C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:FAR8(YMR029C)|FD-Score:-4.48|P-value:3.68E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FCY2(YER056C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FEN1(YCR034W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GAL1(YBR020W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:HBT1(YDL223C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HEF3(YNL014W)|FD-Score:5.29|P-value:6.02E-8||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:ILV6(YCL009C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IRC6(YFR043C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE6(YPR159W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MBR1(YKL093W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MDL1(YLR188W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MFA2(YNL145W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MKT1(YNL085W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MLS1(YNL117W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MPD2(YOL088C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:NTC20(YBR188C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:NUP170(YBL079W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PAD1(YDR538W)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PCL9(YDL179W)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:RAD10(YML095C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:REC107(YJR021C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:SHH4(YLR164W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SLM5(YCR024C)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPT4(YGR063C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR2(YDR297W)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TCB1(YOR086C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRM732(YMR259C_p)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UBA3(YPR066W)|FD-Score:4.82|P-value:7.26E-7||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VEL1(YGL258W)|FD-Score:-4.07|P-value:2.40E-5||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VID22(YLR373C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBL044W(YBL044W_p)|FD-Score:-5.85|P-value:2.39E-9||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YBR226C(YBR226C_d)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR089W(YDR089W_p)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YDR415C(YDR415C_p)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Putative aminopeptidase Gene:YEA6(YEL006W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YET1(YKL065C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGL010W(YGL010W_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR053C(YGR053C_p)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLL059C(YLL059C_d)|FD-Score:4.82|P-value:7.32E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function Gene:YOR345C(YOR345C_d)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YOR378W(YOR378W_p)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:ALY2(YJL084C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:COQ8(YGL119W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:CPA2(YJR109C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CYB2(YML054C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DIA3(YDL024C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:FAR8(YMR029C)|FD-Score:-4.48|P-value:3.68E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FCY2(YER056C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress Gene:FEN1(YCR034W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GAL1(YBR020W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:HBT1(YDL223C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HEF3(YNL014W)|FD-Score:5.29|P-value:6.02E-8||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:ILV6(YCL009C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IRC6(YFR043C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE6(YPR159W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MBR1(YKL093W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MDL1(YLR188W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MFA2(YNL145W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MKT1(YNL085W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MLS1(YNL117W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MPD2(YOL088C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:NTC20(YBR188C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:NUP170(YBL079W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PAD1(YDR538W)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PCL9(YDL179W)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:RAD10(YML095C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:REC107(YJR021C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:SHH4(YLR164W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SLM5(YCR024C)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPT4(YGR063C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR2(YDR297W)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TCB1(YOR086C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRM732(YMR259C_p)|FD-Score:-3.93|P-value:4.18E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:UBA3(YPR066W)|FD-Score:4.82|P-value:7.26E-7||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VEL1(YGL258W)|FD-Score:-4.07|P-value:2.40E-5||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VID22(YLR373C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBL044W(YBL044W_p)|FD-Score:-5.85|P-value:2.39E-9||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YBR226C(YBR226C_d)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR089W(YDR089W_p)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YDR415C(YDR415C_p)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Putative aminopeptidase Gene:YEA6(YEL006W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YET1(YKL065C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGL010W(YGL010W_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGR053C(YGR053C_p)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLL059C(YLL059C_d)|FD-Score:4.82|P-value:7.32E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function Gene:YOR345C(YOR345C_d)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YOR378W(YOR378W_p)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR240C4.621.92E-60.73SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YFR050C3.895.06E-50.08PRE4Beta 7 subunit of the 20S proteasome
YGR119C3.816.96E-50.07NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YLR008C3.749.26E-50.02PAM18Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
YGR128C3.721.02E-40.03UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR328C3.691.12E-40.03SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YDL141W3.661.25E-40.07BPL1Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
YLR076C_d3.601.62E-40.03YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YOL038W3.561.82E-40.27PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YKL083W_d3.304.89E-40.04YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YDR047W3.265.64E-40.00HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YGL097W3.255.73E-40.20SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YBR002C3.060.001120.07RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YLR075W2.990.001390.01RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YPR136C_d2.990.001420.06YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL014W5.296.02E-8HEF3Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication
YOL088C5.181.09E-7MPD2Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
YER056C5.141.35E-7FCY2Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
YPR066W4.827.26E-7UBA3Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YLL059C_d4.827.32E-7YLL059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL093W4.405.41E-6MBR1Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants
YOR316C4.261.02E-5COT1Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
YGR063C4.221.21E-5SPT4Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
YBR226C_d4.131.82E-5YBR226C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W
YML082W_p4.042.65E-5YML082W_pPutative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene
YDL024C4.022.86E-5DIA3Protein of unknown function, involved in invasive and pseudohyphal growth
YOR086C4.013.08E-5TCB1Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
YGR053C_p3.934.31E-5YGR053C_pPutative protein of unknown function
YDL179W3.797.46E-5PCL9Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p
YJL084C3.778.26E-5ALY2Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_816
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2002.33E-54SGTC_8184072-2707 53.5 μMChemDiv (Drug-like library)8828710.0263158
0.1863.16E-47SGTC_8171391-0715 46.0 μMChemDiv (Drug-like library)13539460.0178571
0.1151.08E-18SGTC_490phenamil 164.0 μMMiscellaneous47550.0444444heme requiring
0.1041.38E-15SGTC_9661300-0360 30.2 μMChemDiv (Drug-like library)705880.0277778heme requiring
0.0946.03E-13SGTC_2739tacrine 100.9 μMMiscellaneous19350.0277778heme requiring
0.0903.66E-12SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.053571460S ribosome export
0.0855.71E-11SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.125superoxide
0.0857.58E-11SGTC_11433454-2408 5.1 μMChemDiv (Drug-like library)X11430.018181860S ribosome export
0.0831.68E-10SGTC_29137966556 36.6 μMChembridge (Drug-like library)29765160.035714360S ribosome export
0.0723.47E-8SGTC_22077258803 200.0 μMChembridge (Fragment library)36667990.117647
0.0723.42E-8SGTC_273hydroxyurea 18.1 mMMiscellaneous36570.0526316heme biosynthesis & mitochondrial translocase
0.0699.65E-8SGTC_2645abamectin 100.0 μMMicrosource (Natural product library)57022080.0285714heme requiring
0.0691.05E-7SGTC_32439134147 49.5 μMChembridge (Drug-like library)242821800.047619
0.0681.57E-7SGTC_2840988-0104 32.6 μMChemDiv (Drug-like library)46665320.0444444heme requiring
0.0681.95E-7SGTC_32879117173 37.1 μMChembridge (Drug-like library)27897090.021739160S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_162k030-001537.8 μM0.2222221809920ChemDiv (Drug-like library)304.922922.16712iron homeostasis
SGTC_20545233173129 μM0.2222226889Chembridge (Fragment library)226.026861.41812mitochondrial response to ROS
SGTC_1459k035-003135.7 μM0.2068973877128ChemDiv (Drug-like library)260.471922.08312DNA intercalators
SGTC_1069diphenylcyclopropenone8.49 μM0.265057NIH Clinical Collection206.23933.30101
SGTC_1891st05907326.2 μM0.193548688754TimTec (Natural product derivative library)243.0292632.04603TSC3-RPN4
SGTC_110438-03071.3 μM0.1904764011476ChemDiv (Drug-like library)299.0054133.31706
SGTC_23469031566143.75 μM0.1785714771086Chembridge (Fragment library)256.09921.10612Golgi
SGTC_1060092-000311.58 μM0.1764711072148ChemDiv (Drug-like library)355.182143.67503copper-dependent oxidative stress
SGTC_544k015-002052.4 μM0.1764713008304ChemDiv (Drug-like library)316.14943.20802
SGTC_1693xanthone101.9 μM0.1739137020TimTec (Natural product derivative library)196.201422.96402DNA damage response