1013-0258

(5E)-3-[(4-chloroanilino)methyl]-5-[(4-methoxyphenyl)methylidene]-2-sulfanylidene-1,3-thiazolidin-4-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_819
Screen concentration 27.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 5753198
SMILES COC1=CC=C(C=C1)C=C2C(=O)N(C(=S)S2)CNC3=CC=C(C=C3)Cl
Standardized SMILES COc1ccc(C=C2SC(=S)N(CNc3ccc(Cl)cc3)C2=O)cc1
Molecular weight 390.9069
ALogP 5.13
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.68
% growth inhibition (Hom. pool) 14.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5753198
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:-3.33|P-value:4.41E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:-3.35|P-value:4.09E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DBP9(YLR276C)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.04||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:HEM3(YDL205C)|FD-Score:-6.48|P-value:4.50E-11|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:JAC1(YGL018C)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:MEX67(YPL169C)|FD-Score:-3.46|P-value:2.69E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:PMA1(YGL008C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.13||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:PRE5(YMR314W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.01||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RAT1(YOR048C)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.26||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RPN8(YOR261C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPT1(YKL145W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.37||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSP5(YER125W)|FD-Score:-3.5|P-value:2.36E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC13(YLR208W)|FD-Score:6.62|P-value:1.80E-11|Clearance:2.1||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC27(YGL137W)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.25||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SRP101(YDR292C)|FD-Score:4.52|P-value:3.07E-6|Clearance:0.33||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:TRE2(YOR256C)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.06||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:CCT2(YIL142W)|FD-Score:-3.33|P-value:4.41E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:-3.35|P-value:4.09E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DBP9(YLR276C)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.04||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:HEM3(YDL205C)|FD-Score:-6.48|P-value:4.50E-11|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:JAC1(YGL018C)|FD-Score:-3.68|P-value:1.17E-4|Clearance:0||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:MEX67(YPL169C)|FD-Score:-3.46|P-value:2.69E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:PMA1(YGL008C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.13||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:PRE5(YMR314W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.01||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RAT1(YOR048C)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.26||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RPN8(YOR261C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPT1(YKL145W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.37||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSP5(YER125W)|FD-Score:-3.5|P-value:2.36E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC13(YLR208W)|FD-Score:6.62|P-value:1.80E-11|Clearance:2.1||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC27(YGL137W)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.25||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SRP101(YDR292C)|FD-Score:4.52|P-value:3.07E-6|Clearance:0.33||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:TRE2(YOR256C)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.06||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5753198
Download HOP data (tab-delimited text)  (excel)
Gene:AHT1(YHR093W_d)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARO1(YDR127W)|FD-Score:10.4|P-value:1.33E-25||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:8.93|P-value:2.08E-19||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BGL2(YGR282C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BIO4(YNR057C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BRP1(YGL007W_d)|FD-Score:7.82|P-value:2.56E-15||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BST1(YFL025C)|FD-Score:6.98|P-value:1.46E-12||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCP1(YKR066C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CHS7(YHR142W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CHZ1(YER030W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CKA2(YOR061W)|FD-Score:4.31|P-value:8.07E-6||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLC1(YGR167W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CMP2(YML057W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CSM4(YPL200W)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTK3(YML112W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CUR1(YPR158W)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DHH1(YDL160C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DIA3(YDL024C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ERD1(YDR414C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG2(YMR202W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAR10(YLR238W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FAU1(YER183C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FKS1(YLR342W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GCN2(YDR283C)|FD-Score:9.31|P-value:6.23E-21||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:9.18|P-value:2.18E-20||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:8.95|P-value:1.74E-19||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:10.5|P-value:7.29E-26||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDA1(YEL042W)|FD-Score:7.57|P-value:1.91E-14||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GTR2(YGR163W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HEF3(YNL014W)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HIM1(YDR317W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HOS2(YGL194C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HXT17(YNR072W)|FD-Score:5.23|P-value:8.66E-8||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:ITC1(YGL133W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KNH1(YDL049C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LGE1(YPL055C)|FD-Score:4.31|P-value:8.15E-6||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MAD2(YJL030W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MLS1(YNL117W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL22(YNL177C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NBP2(YDR162C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:OPI3(YJR073C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PET117(YER058W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET127(YOR017W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHO88(YBR106W)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIF1(YML061C)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RCR1(YBR005W)|FD-Score:4.86|P-value:5.81E-7||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:RDS1(YCR106W)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RPL2B(YIL018W)|FD-Score:-3.9|P-value:4.74E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPN4(YDL020C)|FD-Score:-3.93|P-value:4.19E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS11A(YDR025W)|FD-Score:-4.78|P-value:8.58E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SDT1(YGL224C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SIP3(YNL257C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SMF3(YLR034C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SPE2(YOL052C)|FD-Score:-3.87|P-value:5.39E-5||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPI1(YER150W)|FD-Score:-3.77|P-value:8.09E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SUC2(YIL162W)|FD-Score:4.78|P-value:8.74E-7||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUR2(YDR297W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWD1(YAR003W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:TRP1(YDR007W)|FD-Score:13.4|P-value:3.32E-41||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8.61|P-value:3.61E-18||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:19.1|P-value:8.80E-82||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.74|P-value:1.03E-22||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:9.64|P-value:2.65E-22||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBA3(YPR066W)|FD-Score:-5.52|P-value:1.69E-8||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBR1(YGR184C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VPS13(YLL040C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:XBP1(YIL101C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL118W(YDL118W_p)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL172C(YDL172C_d)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:8.28|P-value:6.30E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Putative protein of unknown function Gene:YGL117W(YGL117W_p)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative protein of unknown function Gene:YGR026W(YGR026W_p)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR139W(YGR139W_d)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL147C(YJL147C_p)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL077W(YKL077W_p)|FD-Score:8|P-value:6.04E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR125W(YLR125W_p)|FD-Score:6.12|P-value:4.65E-10||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YMR102C(YMR102C_p)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL029C(YOL029C_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL225W(YPL225W)|FD-Score:5.9|P-value:1.80E-9||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YSP1(YHR155W)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:AHT1(YHR093W_d)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARO1(YDR127W)|FD-Score:10.4|P-value:1.33E-25||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:8.93|P-value:2.08E-19||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BGL2(YGR282C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BIO4(YNR057C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BRP1(YGL007W_d)|FD-Score:7.82|P-value:2.56E-15||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BST1(YFL025C)|FD-Score:6.98|P-value:1.46E-12||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCP1(YKR066C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CHS7(YHR142W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CHZ1(YER030W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CKA2(YOR061W)|FD-Score:4.31|P-value:8.07E-6||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLC1(YGR167W)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CMP2(YML057W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CSM4(YPL200W)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTK3(YML112W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CUR1(YPR158W)|FD-Score:4.39|P-value:5.66E-6||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DHH1(YDL160C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DIA3(YDL024C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ERD1(YDR414C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG2(YMR202W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAR10(YLR238W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FAU1(YER183C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FKS1(YLR342W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:GCN2(YDR283C)|FD-Score:9.31|P-value:6.23E-21||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:9.18|P-value:2.18E-20||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:8.95|P-value:1.74E-19||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:10.5|P-value:7.29E-26||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDA1(YEL042W)|FD-Score:7.57|P-value:1.91E-14||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GTR2(YGR163W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HEF3(YNL014W)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HIM1(YDR317W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HOS2(YGL194C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HXT17(YNR072W)|FD-Score:5.23|P-value:8.66E-8||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:ITC1(YGL133W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KNH1(YDL049C)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LGE1(YPL055C)|FD-Score:4.31|P-value:8.15E-6||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MAD2(YJL030W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MLS1(YNL117W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL22(YNL177C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NBP2(YDR162C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:OPI3(YJR073C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PET117(YER058W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET127(YOR017W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHO88(YBR106W)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIF1(YML061C)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RCR1(YBR005W)|FD-Score:4.86|P-value:5.81E-7||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:RDS1(YCR106W)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RPL2B(YIL018W)|FD-Score:-3.9|P-value:4.74E-5||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPN4(YDL020C)|FD-Score:-3.93|P-value:4.19E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS11A(YDR025W)|FD-Score:-4.78|P-value:8.58E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SDT1(YGL224C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SIP3(YNL257C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SMF3(YLR034C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SPE2(YOL052C)|FD-Score:-3.87|P-value:5.39E-5||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPI1(YER150W)|FD-Score:-3.77|P-value:8.09E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SUC2(YIL162W)|FD-Score:4.78|P-value:8.74E-7||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUR2(YDR297W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWD1(YAR003W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:TRP1(YDR007W)|FD-Score:13.4|P-value:3.32E-41||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8.61|P-value:3.61E-18||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:19.1|P-value:8.80E-82||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.74|P-value:1.03E-22||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:9.64|P-value:2.65E-22||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBA3(YPR066W)|FD-Score:-5.52|P-value:1.69E-8||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBR1(YGR184C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VPS13(YLL040C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:XBP1(YIL101C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL118W(YDL118W_p)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL172C(YDL172C_d)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:8.28|P-value:6.30E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Putative protein of unknown function Gene:YGL117W(YGL117W_p)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative protein of unknown function Gene:YGR026W(YGR026W_p)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR139W(YGR139W_d)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL147C(YJL147C_p)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL077W(YKL077W_p)|FD-Score:8|P-value:6.04E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR125W(YLR125W_p)|FD-Score:6.12|P-value:4.65E-10||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YMR102C(YMR102C_p)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:3.77|P-value:8.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL029C(YOL029C_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL225W(YPL225W)|FD-Score:5.9|P-value:1.80E-9||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YSP1(YHR155W)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W6.621.80E-112.10SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YDR292C4.523.07E-60.33SRP101Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
YOR261C4.191.41E-50.01RPN8Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
YKL145W4.181.45E-50.37RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YGL137W3.816.90E-50.25SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YGL008C3.561.85E-40.13PMA1Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
YLR276C3.432.99E-40.04DBP9DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
YMR314W3.393.46E-40.01PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YOR048C3.383.60E-40.26RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YOR256C3.129.05E-40.06TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YBL097W3.060.001100.04BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YIL115C3.020.001260.13NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YER148W2.890.001940.06SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YBR160W2.820.002380.04CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YKL154W2.780.002690.02SRP102Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C19.108.80E-82TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR007W13.403.32E-41TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YEL009C10.507.29E-26GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR127W10.401.33E-25ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR354W9.741.03E-22TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGL026C9.642.65E-22TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YDR283C9.316.23E-21GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YFR009W9.182.18E-20GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YKR026C8.951.74E-19GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YGL148W8.932.08E-19ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YER090W8.613.61E-18TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR008C_d8.286.30E-17YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL077W_p8.006.04E-16YKL077W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YGL007W_d7.822.56E-15BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YEL042W7.571.91E-14GDA1Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate

GO enrichment analysis for SGTC_819
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5250SGTC_18825615643 20.0 μMMiscellaneous22530750.590164TRP & mitochondrial translation
0.5250SGTC_24885268135 45.7 μMMiscellaneous12703560.179104
0.5190SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0625
0.5170SGTC_18815652484 16.0 μMMiscellaneous22551290.315068TRP & mitochondrial translation
0.5160SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.114943
0.5150SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0625
0.5130SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.25TRP & mitochondrial translation
0.5020SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0454545
0.5020SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0493827
0.4980SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.265625
0.4940SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.188406
0.4880SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0958904
0.4820SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.029703
0.4640SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0493827
0.4626.89E-310SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0853659

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1882561564320 μM0.5901642253075Miscellaneous392.8979635.84215TRP & mitochondrial translation
SGTC_242856156437.78 μM0.5901642253075Miscellaneous392.8979635.84215
SGTC_2447574174233.78 μM0.4923081776504Miscellaneous433.318884.80515
SGTC_2453573113222.18 μM0.451362040Miscellaneous436.266794.16614
SGTC_5203584-0023682 μM0.4166671340790ChemDiv (Drug-like library)293.361342.81505
SGTC_2445574259735.57 μM0.3888891774255Miscellaneous433.318884.80515
SGTC_9403232-128754.8 μM0.3857141585493ChemDiv (Drug-like library)380.48172.87607NEO1
SGTC_2433560787427.82 μM0.3823532252428Miscellaneous374.84134.23515
SGTC_2426558204632.21 μM0.3802822251934Miscellaneous386.3968033.64817
SGTC_24375741531144.16 μM0.3752262990Miscellaneous402.4392634.24617
SGTC_2451585622345.7 μM0.3692312179896Miscellaneous269.339942.31105