1114-0266

5-(2-bromoanilino)-3-phenyl-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_821
Screen concentration 29.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 3123235
SMILES C1=CC=C(C=C1)N2C(=O)C(SC2=O)NC3=CC=CC=C3Br
Standardized SMILES Brc1ccccc1NC2SC(=O)N(C2=O)c3ccccc3
Molecular weight 363.229
ALogP 4.45
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.55
% growth inhibition (Hom. pool) 3.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3123235
Download HIP data (tab-delimited text)  (excel)
Gene:CDC6(YJL194W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.02||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DBP2(YNL112W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.05||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DIM1(YPL266W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:MYO2(YOR326W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.23||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:PSF2(YJL072C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPP1(YHR062C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC13(YLR208W)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAF12(YDR145W)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.47||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFB4(YPR056W)|FD-Score:4.82|P-value:7.32E-7|Clearance:0.23||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TOM22(YNL131W)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.09||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YCS4(YLR272C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YHR020W(YHR020W)|FD-Score:7.23|P-value:2.38E-13|Clearance:2.42||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:CDC6(YJL194W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.02||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DBP2(YNL112W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.05||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DIM1(YPL266W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:MYO2(YOR326W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.23||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:PSF2(YJL072C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPP1(YHR062C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC13(YLR208W)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAF12(YDR145W)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.47||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TFB4(YPR056W)|FD-Score:4.82|P-value:7.32E-7|Clearance:0.23||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TOM22(YNL131W)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.09||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YCS4(YLR272C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YHR020W(YHR020W)|FD-Score:7.23|P-value:2.38E-13|Clearance:2.42||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3123235
Download HOP data (tab-delimited text)  (excel)
Gene:AKR1(YDR264C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:APQ13(YJL075C_d)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARO3(YDR035W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:CCE1(YKL011C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CKA1(YIL035C)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CMP2(YML057W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COG8(YML071C)|FD-Score:5.65|P-value:7.91E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL3(YIR032C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DSK2(YMR276W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:ELP6(YMR312W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMP47(YFL048C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:EXG2(YDR261C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FCY22(YER060W-A)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FPR4(YLR449W)|FD-Score:5.58|P-value:1.21E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GCN4(YEL009C)|FD-Score:5.23|P-value:8.68E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GGA2(YHR108W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GMH1(YKR030W)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GUP1(YGL084C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HBT1(YDL223C)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HEK2(YBL032W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HRD3(YLR207W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:IRC20(YLR247C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:MDL1(YLR188W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MPM1(YJL066C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MVB12(YGR206W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NTH2(YBR001C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OST6(YML019W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PEX7(YDR142C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PKP2(YGL059W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMP1(YCR024C-A)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUF4(YGL014W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RCY1(YJL204C)|FD-Score:4.34|P-value:7.13E-6||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:SFB3(YHR098C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGA1(YIL099W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SHE2(YKL130C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:TAT2(YOL020W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:THI21(YPL258C)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TPK2(YPL203W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP1(YDR007W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.21|P-value:2.60E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.39|P-value:5.73E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5|P-value:2.90E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:URA2(YJL130C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VPS52(YDR484W)|FD-Score:4.89|P-value:4.95E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:3.73|P-value:9.41E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR016W(YBR016W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR284W(YBR284W_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR401W(YDR401W_d)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YGR026W(YGR026W_p)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YHR140W(YHR140W_p)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIA6(YIL006W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YKR011C(YKR011C_p)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YML082W(YML082W_p)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL143C(YNL143C_p)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL235C(YNL235C_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL057W(YOL057W)|FD-Score:-3.84|P-value:6.22E-5||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL073C(YOL073C_p)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR318C(YOR318C_d)|FD-Score:-3.93|P-value:4.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPL191C(YPL191C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT6(YLR262C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:6.12|P-value:4.76E-10||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:AKR1(YDR264C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:APQ13(YJL075C_d)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARO3(YDR035W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:CCE1(YKL011C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CKA1(YIL035C)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CMP2(YML057W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COG8(YML071C)|FD-Score:5.65|P-value:7.91E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL3(YIR032C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DSK2(YMR276W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:ELP6(YMR312W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMP47(YFL048C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:EXG2(YDR261C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FCY22(YER060W-A)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FPR4(YLR449W)|FD-Score:5.58|P-value:1.21E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GCN4(YEL009C)|FD-Score:5.23|P-value:8.68E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GGA2(YHR108W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GMH1(YKR030W)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GUP1(YGL084C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HBT1(YDL223C)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HEK2(YBL032W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HRD3(YLR207W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:IRC20(YLR247C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:MDL1(YLR188W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MPM1(YJL066C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MVB12(YGR206W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NTH2(YBR001C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OST6(YML019W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PEX7(YDR142C)|FD-Score:4.37|P-value:6.09E-6||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PKP2(YGL059W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMP1(YCR024C-A)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUF4(YGL014W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RCY1(YJL204C)|FD-Score:4.34|P-value:7.13E-6||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:SFB3(YHR098C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGA1(YIL099W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SHE2(YKL130C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:TAT2(YOL020W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:THI21(YPL258C)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TPK2(YPL203W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP1(YDR007W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.21|P-value:2.60E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.39|P-value:5.73E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5|P-value:2.90E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:URA2(YJL130C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VPS52(YDR484W)|FD-Score:4.89|P-value:4.95E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:3.73|P-value:9.41E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR016W(YBR016W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR284W(YBR284W_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR401W(YDR401W_d)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YGR026W(YGR026W_p)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YHR140W(YHR140W_p)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIA6(YIL006W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YKR011C(YKR011C_p)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YML082W(YML082W_p)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL143C(YNL143C_p)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL235C(YNL235C_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL057W(YOL057W)|FD-Score:-3.84|P-value:6.22E-5||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL073C(YOL073C_p)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR318C(YOR318C_d)|FD-Score:-3.93|P-value:4.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPL191C(YPL191C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT6(YLR262C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:6.12|P-value:4.76E-10||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR020W7.232.38E-132.42YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YPR056W4.827.32E-70.23TFB4Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
YDR145W4.592.23E-60.47TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YHR062C4.121.89E-50.48RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR326W3.641.39E-40.23MYO2Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
YNL112W3.413.28E-40.05DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YJL194W3.363.93E-40.02CDC6Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YNL131W3.344.24E-40.09TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YLR208W3.245.88E-40.21SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YDR292C3.040.001190.05SRP101Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
YKL006C-A2.980.001420.04SFT1Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
YOL010W2.950.001600.04RCL1Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
YJR068W2.910.001830.03RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YOR361C2.880.001990.10PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YOL130W2.780.002720.03ALR1Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C6.212.60E-10TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YHR017W6.124.76E-10YSC83Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
YML071C5.657.91E-9COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR449W5.581.21E-8FPR4Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
YEL009C5.238.68E-8GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YML082W_p5.141.41E-7YML082W_pPutative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene
YGL026C5.002.90E-7TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YDR484W4.894.95E-7VPS52Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
YKR030W4.885.29E-7GMH1Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting
YKL011C4.681.43E-6CCE1Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
YOL073C_p4.473.94E-6YOL073C_pSubunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2
YKR013W4.464.11E-6PRY2Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication
YDR354W4.395.73E-6TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR142C4.376.09E-6PEX7Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
YJL204C4.347.13E-6RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth

GO enrichment analysis for SGTC_821
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2541.40E-87SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0735294
0.2523.61E-86SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0178571
0.2386.48E-77SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.145161
0.2262.76E-69SGTC_18825615643 20.0 μMMiscellaneous22530750.103896TRP & mitochondrial translation
0.2252.23E-68SGTC_18815652484 16.0 μMMiscellaneous22551290.183099TRP & mitochondrial translation
0.2231.09E-67SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.206349
0.2221.25E-66SGTC_24785763493 32.3 μMMiscellaneous13655760.0909091
0.2182.66E-64SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.0810811
0.2159.84E-63SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.09375
0.2131.90E-61SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0869565
0.2106.24E-60SGTC_7551995-0008 37.3 μMChemDiv (Drug-like library)6895330.0847458
0.2094.35E-59SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0967742
0.2081.04E-58SGTC_24885268135 45.7 μMMiscellaneous12703560.155172
0.2061.41E-57SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.101449
0.2038.03E-56SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.0481928

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13021159-0335255 μM0.659091649284ChemDiv (Drug-like library)314.358963.68715
SGTC_2103537813810 μM0.326087244142Chembridge (Fragment library)241.242040.84403
SGTC_11791021-14319.29 μM0.3207552840958ChemDiv (Drug-like library)362.240983.81603
SGTC_8850865-010616.8 μM0.3061222793380ChemDiv (Drug-like library)239.678142.47603TSC3-RPN4
SGTC_6811642-000626.6 μM0.2741944446530ChemDiv (Drug-like library)461.599027.51215
SGTC_2474532157043.32 μM0.2727272841384Miscellaneous297.37153.55303
SGTC_5891227-007034.1 μM0.2727272841384ChemDiv (Drug-like library)297.37153.55303
SGTC_20985376439882.5 nM0.2678572844116Chembridge (Fragment library)255.268621.04903
SGTC_14873970-07952.82 μM0.2641513765103ChemDiv (Drug-like library)320.13811.59203DNA intercalators
SGTC_14863970-07934.53 μM0.262844330ChemDiv (Drug-like library)320.13811.59203