3253-0474

N-[(Z)-[(Z)-2-chloro-3-phenylprop-2-enylidene]amino]-2-methylfuran-3-carboxamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_827
Screen concentration 693.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5402065
SMILES CC1=C(C=CO1)C(=O)NN=CC(=CC2=CC=CC=C2)Cl
Standardized SMILES Cc1occc1C(=O)NN=CC(=Cc2ccccc2)Cl
Molecular weight 288.7289
ALogP 2.66
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.29
% growth inhibition (Hom. pool) 7.95


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5402065
Download HIP data (tab-delimited text)  (excel)
Gene:COF1(YLL050C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.27||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CSE1(YGL238W)|FD-Score:7.8|P-value:2.98E-15|Clearance:2.26||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:MOT1(YPL082C)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.02||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NUP57(YGR119C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.2||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PRE4(YFR050C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.02||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RTT105(YER104W)|FD-Score:4.04|P-value:2.70E-5|Clearance:0.16||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC1(YDR164C)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.07||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SKP1(YDR328C)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.02||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SRP102(YKL154W)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.19||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:TUB1(YML085C)|FD-Score:3.88|P-value:5.27E-5|Clearance:0.21||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP11(YKL099C)|FD-Score:-3.87|P-value:5.38E-5|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YAE1(YJR067C)|FD-Score:-3.11|P-value:9.27E-4|Clearance:0||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YBR190W(YBR190W_d)|FD-Score:6.79|P-value:5.68E-12|Clearance:2.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YJR012C(YJR012C_p)|FD-Score:4.53|P-value:2.99E-6|Clearance:0.28||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:COF1(YLL050C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.27||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CSE1(YGL238W)|FD-Score:7.8|P-value:2.98E-15|Clearance:2.26||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:MOT1(YPL082C)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.02||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NUP57(YGR119C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.2||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PRE4(YFR050C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.02||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RTT105(YER104W)|FD-Score:4.04|P-value:2.70E-5|Clearance:0.16||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC1(YDR164C)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.07||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SKP1(YDR328C)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.02||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SRP102(YKL154W)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.19||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:TUB1(YML085C)|FD-Score:3.88|P-value:5.27E-5|Clearance:0.21||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP11(YKL099C)|FD-Score:-3.87|P-value:5.38E-5|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YAE1(YJR067C)|FD-Score:-3.11|P-value:9.27E-4|Clearance:0||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YBR190W(YBR190W_d)|FD-Score:6.79|P-value:5.68E-12|Clearance:2.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YJR012C(YJR012C_p)|FD-Score:4.53|P-value:2.99E-6|Clearance:0.28||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5402065
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM46(YHR199C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APJ1(YNL077W)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARE2(YNR019W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARG5,6(YER069W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ATP10(YLR393W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:ATX1(YNL259C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:CSR2(YPR030W)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTR1(YPR124W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DOM34(YNL001W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DSF2(YBR007C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUR1,2(YBR208C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:ECM19(YLR390W)|FD-Score:7.55|P-value:2.21E-14||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EFT2(YDR385W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:ERG5(YMR015C)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAA3(YIL009W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FAT1(YBR041W)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FMS1(YMR020W)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:GTO3(YMR251W)|FD-Score:5.23|P-value:8.49E-8||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HFD1(YMR110C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:IDP2(YLR174W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:INM2(YDR287W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:LTV1(YKL143W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MAE1(YKL029C)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MAL33(YBR297W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCM22(YJR135C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MDS3(YGL197W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MMR1(YLR190W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MNE1(YOR350C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:PDR16(YNL231C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PET10(YKR046C)|FD-Score:4.32|P-value:7.89E-6||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PMU1(YKL128C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PRD1(YCL057W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RAD59(YDL059C)|FD-Score:-4.49|P-value:3.64E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RAV2(YDR202C)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REF2(YDR195W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:ROX3(YBL093C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL43B(YJR094W-A)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT7(YLL030C_d)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:SIZ1(YDR409W)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SQS1(YNL224C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SSK1(YLR006C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TAT2(YOL020W)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TGS1(YPL157W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TMA19(YKL056C)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:UBC5(YDR059C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:UBP5(YER144C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VAC7(YNL054W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VMA16(YHR026W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YCK3(YER123W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR124W(YDR124W_p)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YHR202W(YHR202W_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL163C(YIL163C_p)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR003C(YJR003C_p)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YJR115W(YJR115W_p)|FD-Score:5.29|P-value:6.23E-8||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKL044W(YKL044W_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL131W(YKL131W_d)|FD-Score:5.61|P-value:9.97E-9||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL285W(YNL285W_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL019W-A(YOL019W-A_p)|FD-Score:4.82|P-value:7.03E-7||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOL075C(YOL075C_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative ABC transporter Gene:YOL085C(YOL085C_d)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR019W(YOR019W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR331C(YOR331C_d)|FD-Score:5.49|P-value:2.06E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:ADK1(YDR226W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM46(YHR199C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APJ1(YNL077W)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARE2(YNR019W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARG5,6(YER069W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ATP10(YLR393W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:ATX1(YNL259C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:CSR2(YPR030W)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTR1(YPR124W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DOM34(YNL001W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DSF2(YBR007C_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUR1,2(YBR208C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:ECM19(YLR390W)|FD-Score:7.55|P-value:2.21E-14||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EFT2(YDR385W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:ERG5(YMR015C)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FAA3(YIL009W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FAT1(YBR041W)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FMS1(YMR020W)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:GTO3(YMR251W)|FD-Score:5.23|P-value:8.49E-8||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HFD1(YMR110C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:IDP2(YLR174W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:INM2(YDR287W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:LTV1(YKL143W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MAE1(YKL029C)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MAL33(YBR297W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCM22(YJR135C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MDS3(YGL197W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MMR1(YLR190W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MNE1(YOR350C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:PDR16(YNL231C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PET10(YKR046C)|FD-Score:4.32|P-value:7.89E-6||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PMU1(YKL128C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PRD1(YCL057W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RAD59(YDL059C)|FD-Score:-4.49|P-value:3.64E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RAV2(YDR202C)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REF2(YDR195W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:ROX3(YBL093C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL43B(YJR094W-A)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT7(YLL030C_d)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:SIZ1(YDR409W)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SQS1(YNL224C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SSK1(YLR006C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TAT2(YOL020W)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TGS1(YPL157W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TMA19(YKL056C)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:UBC5(YDR059C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:UBP5(YER144C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VAC7(YNL054W)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VMA16(YHR026W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YCK3(YER123W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR124W(YDR124W_p)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YHR202W(YHR202W_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIL163C(YIL163C_p)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR003C(YJR003C_p)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YJR115W(YJR115W_p)|FD-Score:5.29|P-value:6.23E-8||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKL044W(YKL044W_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL131W(YKL131W_d)|FD-Score:5.61|P-value:9.97E-9||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL285W(YNL285W_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL019W-A(YOL019W-A_p)|FD-Score:4.82|P-value:7.03E-7||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOL075C(YOL075C_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative ABC transporter Gene:YOL085C(YOL085C_d)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR019W(YOR019W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOR331C(YOR331C_d)|FD-Score:5.49|P-value:2.06E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL238W7.802.98E-152.26CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YBR190W_d6.795.68E-122.26YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YJR012C_p4.532.99E-60.28YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YDR328C4.251.07E-50.02SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YKL154W4.231.19E-50.19SRP102Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
YER104W4.042.70E-50.16RTT105Protein with a role in regulation of Ty1 transposition
YML085C3.885.27E-50.21TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YFR050C3.661.24E-40.02PRE4Beta 7 subunit of the 20S proteasome
YGR119C3.641.35E-40.20NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YPL082C3.452.85E-40.02MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YDR164C3.433.07E-40.07SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
YLL050C3.363.92E-40.27COF1Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
YLR310C3.090.001010.13CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YJR023C_d2.960.001550.02YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YHR164C2.940.001656.37E-4DNA2Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR390W7.552.21E-14ECM19Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKL131W_d5.619.97E-9YKL131W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR331C_d5.492.06E-8YOR331C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W
YJR115W_p5.296.23E-8YJR115W_pPutative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
YMR251W5.238.49E-8GTO3Omega class glutathione transferase; putative cytosolic localization
YKR098C4.993.09E-7UBP11Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins
YOL019W-A_p4.827.03E-7YOL019W-A_pIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YGL197W4.751.04E-6MDS3Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain
YBL093C4.592.23E-6ROX3Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
YNL077W4.483.74E-6APJ1Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
YKR046C4.327.89E-6PET10Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange
YER144C4.251.07E-5UBP5Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck
YDR124W_p4.171.52E-5YDR124W_pPutative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor
YBR007C_p4.161.62E-5DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YNL001W4.151.64E-5DOM34Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_827
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0612.36E-6SGTC_10553448-0672 86.0 μMChemDiv (Drug-like library)7483780.153846
0.0571.05E-5SGTC_14464239-0190 62.0 μMChemDiv (Drug-like library)13400850.121951
0.0552.83E-5SGTC_246cycloheximide 667.0 μMICCB bioactive library167600400.0410959
0.0543.85E-5SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.135135fatty acid desaturase (OLE1)
0.0535.05E-5SGTC_1063disulfiram 1.8 μMNIH Clinical Collection31170.0363636
0.0511.02E-4SGTC_2546rhodinyl acetate 100.0 μMMicrosource (Natural product library)67086820.0793651
0.0501.31E-4SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.084507
0.0491.90E-4SGTC_20795212404 121.0 μMChembridge (Fragment library)7584620.03125
0.0482.12E-4SGTC_3252810-4341 181.0 μMChemDiv (Drug-like library)7749700.0821918amide catabolism
0.0482.39E-4SGTC_7874152-0075 138.0 μMChemDiv (Drug-like library)95665390.0985916
0.0463.67E-4SGTC_22887946289 167.3 μMChembridge (Fragment library)12460520.15942
0.0463.95E-4SGTC_2585alliin 100.0 μMMicrosource (Natural product library)873100.0634921
0.0455.15E-4SGTC_9483448-0295 75.3 μMChemDiv (Drug-like library)6785690.0895522
0.0455.25E-4SGTC_31429086498 49.5 μMChembridge (Drug-like library)252363670.113924iron homeostasis
0.0446.28E-4SGTC_850868-0259 160.1 μMChemDiv (Drug-like library)831730.1587360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1483253-0744288.01 μM0.5090916893655ChemDiv (Drug-like library)347.20652.89113
SGTC_7051124-0212310 μM0.3818189554485ChemDiv (Drug-like library)293.1484.01212
SGTC_21886126565140.68 μM0.357143874518Chembridge (Fragment library)208.237020.86114mitochondrial processes
SGTC_6541124-027180.9 μM0.3015875403748ChemDiv (Drug-like library)309.115462.58524
SGTC_30833-058795.58 μM0.2857145382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_12011889-337245.9 μM0.2857145392199ChemDiv (Drug-like library)282.337023.49113Golgi
SGTC_24605552655174.22 μM0.2833335401989Miscellaneous259.6912.62513amide catabolism
SGTC_7531322-0200289 μM0.2769235403907ChemDiv (Drug-like library)275.216961.85526
SGTC_563tetrahydrosecaminediol614 μM0.268657334274ChemDiv (Drug-like library)624.898248.24734amide catabolism
SGTC_8981000-084928.3 μM0.2656256751807ChemDiv (Drug-like library)356.374042.72334