3852-0053

1-[3-butyl-5-hydroxy-5-(trifluoromethyl)-4H-pyrazol-1-yl]-2-(2-methylphenoxy)ethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_833
Screen concentration 558.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3123681
SMILES CCCCC1=NN(C(C1)(C(F)(F)F)O)C(=O)COC2=CC=CC=C2C
Standardized SMILES CCCCC1=NN(C(=O)COc2ccccc2C)C(O)(C1)C(F)(F)F
Molecular weight 358.3555
ALogP 3.68
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.78
% growth inhibition (Hom. pool) 3.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3123681
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.16||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:DAD2(YKR083C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.04||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP2(YNL112W)|FD-Score:7.2|P-value:2.99E-13|Clearance:1.79||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GPI19(YDR437W)|FD-Score:3.21|P-value:6.65E-4|Clearance:0.06||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:LCB1(YMR296C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.05||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LSM3(YLR438C-A)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.08||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCM2(YBL023C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.02||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:PHS1(YJL097W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:RPC34(YNR003C)|FD-Score:4.82|P-value:7.34E-7|Clearance:0.91||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RTT105(YER104W)|FD-Score:3.87|P-value:5.46E-5|Clearance:0.18||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SPC42(YKL042W)|FD-Score:5.41|P-value:3.21E-8|Clearance:0.59||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAF10(YDR167W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.03||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YGL074C(YGL074C_d)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YJL009W(YJL009W_d)|FD-Score:3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJR012C(YJR012C_p)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.21||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:ABF1(YKL112W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.16||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:DAD2(YKR083C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.04||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP2(YNL112W)|FD-Score:7.2|P-value:2.99E-13|Clearance:1.79||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GPI19(YDR437W)|FD-Score:3.21|P-value:6.65E-4|Clearance:0.06||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:LCB1(YMR296C)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.05||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LSM3(YLR438C-A)|FD-Score:3.12|P-value:9.11E-4|Clearance:0.08||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCM2(YBL023C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.02||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:PHS1(YJL097W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:RPC34(YNR003C)|FD-Score:4.82|P-value:7.34E-7|Clearance:0.91||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RTT105(YER104W)|FD-Score:3.87|P-value:5.46E-5|Clearance:0.18||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SPC42(YKL042W)|FD-Score:5.41|P-value:3.21E-8|Clearance:0.59||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAF10(YDR167W)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.03||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YGL074C(YGL074C_d)|FD-Score:3.9|P-value:4.77E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YJL009W(YJL009W_d)|FD-Score:3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJR012C(YJR012C_p)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.21||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3123681
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:APE2(YKL157W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:APP1(YNL094W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:APQ13(YJL075C_d)|FD-Score:5.8|P-value:3.33E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARG3(YJL088W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO2(YGL148W)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:CCR4(YAL021C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIA4(YHR011W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIT1(YDR403W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:FLD1(YLR404W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:GDA1(YEL042W)|FD-Score:4.85|P-value:6.32E-7||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GLC8(YMR311C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HMO1(YDR174W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HTL1(YCR020W-B)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IDP2(YLR174W)|FD-Score:4.27|P-value:9.79E-6||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:5.23|P-value:8.38E-8||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:ISU2(YOR226C)|FD-Score:-3.91|P-value:4.58E-5||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:LDH1(YBR204C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LST4(YKL176C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRP49(YKL167C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MTG1(YMR097C)|FD-Score:-3.73|P-value:9.42E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MZM1(YDR493W)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NTH2(YBR001C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAC1(YOR269W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAF1(YBR279W)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PAN3(YKL025C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDB1(YBR221C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PTC6(YCR079W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RAD5(YLR032W)|FD-Score:4.35|P-value:6.66E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAV2(YDR202C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:SIP5(YMR140W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SOL2(YCR073W-A)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPS4(YOR313C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SRB8(YCR081W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STL1(YDR536W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:SYM1(YLR251W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TDA8(YAL064C-A_p)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TLG2(YOL018C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA19(YKL056C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRM2(YKR056W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TUP1(YCR084C)|FD-Score:-4.35|P-value:6.78E-6||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:VID22(YLR373C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA13(YPR036W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA22(YHR060W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS34(YLR240W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR053C(YBR053C_p)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR056W(YBR056W_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR178W(YBR178W_d)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCL049C(YCL049C_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR278C(YDR278C_d)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL020C(YEL020C_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YER039C-A(YER039C-A_p)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR111W(YGR111W_p)|FD-Score:4.82|P-value:7.11E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJL007C(YJL007C_d)|FD-Score:-5.42|P-value:2.90E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL206C(YJL206C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YKR011C(YKR011C_p)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLL053C(YLL053C_p)|FD-Score:6.63|P-value:1.64E-11||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YOR029W(YOR029W_d)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL039W(YPL039W_p)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:ADE1(YAR015W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:APE2(YKL157W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:APP1(YNL094W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:APQ13(YJL075C_d)|FD-Score:5.8|P-value:3.33E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARG3(YJL088W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO2(YGL148W)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:CCR4(YAL021C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIA4(YHR011W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIT1(YDR403W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:FLD1(YLR404W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:GDA1(YEL042W)|FD-Score:4.85|P-value:6.32E-7||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GLC8(YMR311C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HBT1(YDL223C)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HMO1(YDR174W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HTL1(YCR020W-B)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IDP2(YLR174W)|FD-Score:4.27|P-value:9.79E-6||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:5.23|P-value:8.38E-8||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:ISU2(YOR226C)|FD-Score:-3.91|P-value:4.58E-5||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:LDH1(YBR204C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LST4(YKL176C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRP49(YKL167C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MTG1(YMR097C)|FD-Score:-3.73|P-value:9.42E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MZM1(YDR493W)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NTH2(YBR001C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAC1(YOR269W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAF1(YBR279W)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PAN3(YKL025C)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDB1(YBR221C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PTC6(YCR079W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RAD5(YLR032W)|FD-Score:4.35|P-value:6.66E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAV2(YDR202C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:SIP5(YMR140W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SOL2(YCR073W-A)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPS4(YOR313C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SRB8(YCR081W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STL1(YDR536W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:SYM1(YLR251W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TDA8(YAL064C-A_p)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TLG2(YOL018C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA19(YKL056C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRM2(YKR056W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TUP1(YCR084C)|FD-Score:-4.35|P-value:6.78E-6||SGD DESC:General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes Gene:VID22(YLR373C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA13(YPR036W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA22(YHR060W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS34(YLR240W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR053C(YBR053C_p)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YBR056W(YBR056W_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR178W(YBR178W_d)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCL049C(YCL049C_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR278C(YDR278C_d)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL020C(YEL020C_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YER039C-A(YER039C-A_p)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR111W(YGR111W_p)|FD-Score:4.82|P-value:7.11E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJL007C(YJL007C_d)|FD-Score:-5.42|P-value:2.90E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL206C(YJL206C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YKR011C(YKR011C_p)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLL053C(YLL053C_p)|FD-Score:6.63|P-value:1.64E-11||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YOR029W(YOR029W_d)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR304C-A(YOR304C-A_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL039W(YPL039W_p)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL112W7.202.99E-131.79DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YKL042W5.413.21E-80.59SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YNR003C4.827.34E-70.91RPC34RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
YGL074C_d3.904.77E-50.03YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YER104W3.875.46E-50.18RTT105Protein with a role in regulation of Ty1 transposition
YMR296C3.691.14E-40.05LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YKL112W3.641.38E-40.16ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YKR083C3.482.53E-40.04DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YJL009W_d3.442.94E-44.14E-4YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YBL023C3.442.95E-40.01MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YJR012C_p3.423.11E-40.21YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YDR437W3.216.65E-40.06GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
YDR167W3.158.21E-40.03TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YLR438C-A3.129.11E-40.08LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YNL038W3.040.001190.00GPI15Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLL053C_p6.631.64E-11YLL053C_pPutative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin
YJL075C_d5.803.33E-9APQ13_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate
YKR011C_p5.482.08E-8YKR011C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
YDR493W5.452.54E-8MZM1Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog
YJL037W5.238.38E-8IRC18Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci
YER039C-A_p4.914.66E-7YER039C-A_pPutative protein of unknown function; YER039C-A is not an essential gene
YEL042W4.856.32E-7GDA1Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate
YGR111W_p4.827.11E-7YGR111W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YML071C4.641.72E-6COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDL223C4.513.24E-6HBT1Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
YLR032W4.356.66E-6RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YLR174W4.279.79E-6IDP2Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication
YKL025C4.082.29E-5PAN3Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
YHR206W4.022.91E-5SKN7Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
YKL176C4.003.22E-5LST4Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface

GO enrichment analysis for SGTC_833
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0961.92E-13SGTC_329k048-0121 21.3 μMChemDiv (Drug-like library)68329020.075RSC & ERG11
0.0872.40E-11SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0752688Golgi
0.0857.46E-11SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.075Golgi
0.0831.50E-10SGTC_22057238770 200.0 μMChembridge (Fragment library)2798670.118421
0.0773.39E-9SGTC_1078idebenone 36.1 μMMiscellaneous36860.125Golgi
0.0714.02E-8SGTC_3660181-0206 4.3 μMChemDiv (Drug-like library)2748740.0447761Golgi
0.0707.15E-8SGTC_8211114-0266 29.2 μMChemDiv (Drug-like library)31232350.0875
0.0691.28E-7SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0666667heme requiring
0.0663.52E-7SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.076087Golgi
0.0657.45E-7SGTC_10258-0098 57.4 μMChemDiv (Drug-like library)67900320.0795455heme biosynthesis & mitochondrial translocase
0.0649.32E-7SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0714286Golgi
0.0631.38E-6SGTC_14133910-0535 34.4 μMChemDiv (Drug-like library)28769510.147727
0.0631.38E-6SGTC_3061672-3436 11.6 μMChemDiv (Drug-like library)67973780.0824742Golgi
0.0621.60E-6SGTC_20044023728 41.9 μMChembridge (Fragment library)3600840.0714286
0.0603.54E-6SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.0759494

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5363852-057810.6 μM0.4637682869208ChemDiv (Drug-like library)443.214574.03317
SGTC_3046909221249.47 μM0.34782625236637Chembridge (Drug-like library)328.3806433.404
SGTC_3156909993149.47 μM0.32857118111053Chembridge (Drug-like library)344.835243.85903
SGTC_12153852-057711.2 μM0.3243242884876ChemDiv (Drug-like library)429.187993.95627
SGTC_2798781363745.45 μM0.323529956368Chembridge (Drug-like library)277.2660263.34714
SGTC_831k061-1017217 μM0.301371240153ChemDiv (Drug-like library)512.553125.3636translation
SGTC_1032k061-101898.7 μM0.301374005393ChemDiv (Drug-like library)540.606285.93526
SGTC_3214534-389065.3 μM0.298701699746ChemDiv (Drug-like library)345.82333.24704RPP1 & pyrimidine depletion
SGTC_3157909927449.47 μM0.27631617217662Chembridge (Drug-like library)339.43143.29104
SGTC_269exalamide29 μM0.2727273316Miscellaneous221.295463.05612ergosterol biosynthesis