0312-0008

(3Z)-3-[(4-chlorophenyl)hydrazinylidene]butan-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_834
Screen concentration 75.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 6385573
SMILES CC(=NNC1=CC=C(C=C1)Cl)C(=O)C
Standardized SMILES CC(=O)C(=NNc1ccc(Cl)cc1)C
Molecular weight 210.6601
ALogP 2.3
H-bond donor count 1
H-bond acceptor count 3
Response signature NEO1

Pool Growth Kinetics
% growth inhibition (Het. pool) 29.17
% growth inhibition (Hom. pool) 7.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6385573
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:4|P-value:3.17E-5|Clearance:0.2||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BRX1(YOL077C)|FD-Score:-4.15|P-value:1.63E-5|Clearance:0||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CDC39(YCR093W)|FD-Score:3.8|P-value:7.09E-5|Clearance:0.22||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:ERG11(YHR007C)|FD-Score:9.75|P-value:9.03E-23|Clearance:3.9||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:10.1|P-value:1.90E-24|Clearance:0.38||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:12.6|P-value:1.84E-36|Clearance:2.42||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ERG7(YHR072W)|FD-Score:5.85|P-value:2.44E-9|Clearance:0.45||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:NEO1(YIL048W)|FD-Score:23.1|P-value:1.42E-118|Clearance:10.57||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PSF3(YOL146W)|FD-Score:5.4|P-value:3.29E-8|Clearance:0.21||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPN11(YFR004W)|FD-Score:-3.68|P-value:1.14E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:3.09|P-value:9.86E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RSC6(YCR052W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.39||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC2(YNL272C)|FD-Score:3.19|P-value:7.02E-4|Clearance:0.02||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:TIM17(YJL143W)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.04||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:UTP6(YDR449C)|FD-Score:3.13|P-value:8.74E-4|Clearance:0.04||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL086C(YJL086C_d)|FD-Score:4.9|P-value:4.71E-7|Clearance:0.9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YPT1(YFL038C)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.29||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:ARP4(YJL081C)|FD-Score:4|P-value:3.17E-5|Clearance:0.2||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BRX1(YOL077C)|FD-Score:-4.15|P-value:1.63E-5|Clearance:0||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CDC39(YCR093W)|FD-Score:3.8|P-value:7.09E-5|Clearance:0.22||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:ERG11(YHR007C)|FD-Score:9.75|P-value:9.03E-23|Clearance:3.9||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:10.1|P-value:1.90E-24|Clearance:0.38||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:12.6|P-value:1.84E-36|Clearance:2.42||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ERG7(YHR072W)|FD-Score:5.85|P-value:2.44E-9|Clearance:0.45||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:NEO1(YIL048W)|FD-Score:23.1|P-value:1.42E-118|Clearance:10.57||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PSF3(YOL146W)|FD-Score:5.4|P-value:3.29E-8|Clearance:0.21||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPN11(YFR004W)|FD-Score:-3.68|P-value:1.14E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:3.09|P-value:9.86E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RSC6(YCR052W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.39||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC2(YNL272C)|FD-Score:3.19|P-value:7.02E-4|Clearance:0.02||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:TIM17(YJL143W)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.04||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:UTP6(YDR449C)|FD-Score:3.13|P-value:8.74E-4|Clearance:0.04||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YJL086C(YJL086C_d)|FD-Score:4.9|P-value:4.71E-7|Clearance:0.9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YPT1(YFL038C)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.29||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6385573
Download HOP data (tab-delimited text)  (excel)
Gene:ARO1(YDR127W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:8.75|P-value:1.03E-18||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASR1(YPR093C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG15(YCR068W)|FD-Score:4.76|P-value:9.64E-7||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATP17(YDR377W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BEM4(YPL161C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BRP1(YGL007W_d)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSC1(YDL037C)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BTS1(YPL069C)|FD-Score:5.79|P-value:3.51E-9||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CCW12(YLR110C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDA1(YLR307W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC50(YCR094W)|FD-Score:6.22|P-value:2.44E-10||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COG5(YNL051W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS1(YNL336W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSG2(YBR036C)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DFG5(YMR238W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DOT6(YER088C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DRS2(YAL026C)|FD-Score:4.3|P-value:8.38E-6||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ENV10(YLR065C)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FET4(YMR319C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCN2(YDR283C)|FD-Score:8.85|P-value:4.27E-19||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:8.23|P-value:9.48E-17||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.93|P-value:2.05E-19||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCN5(YGR252W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:10.4|P-value:1.93E-25||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET2(YER083C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HSP82(YPL240C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HUG1(YML058W-A)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:7.81|P-value:2.79E-15||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:KAR4(YCL055W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KDX1(YKL161C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LOT5(YKL183W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MCK1(YNL307C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MLH3(YPL164C)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRPS35(YGR165W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSB3(YNL293W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NCE102(YPR149W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:ORM2(YLR350W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PEX3(YDR329C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PEX6(YNL329C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PHO88(YBR106W)|FD-Score:7.43|P-value:5.33E-14||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PST1(YDR055W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTK2(YJR059W)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAM1(YDL090C)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCE1(YMR274C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCY1(YJL204C)|FD-Score:11.8|P-value:2.18E-32||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGP1(YDR137W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL35B(YDL136W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX1(YBR228W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SLX9(YGR081C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SOL1(YNR034W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPO23(YBR250W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SPT23(YKL020C)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SWI4(YER111C)|FD-Score:5.78|P-value:3.84E-9||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYM1(YLR251W)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TOS6(YNL300W_p)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRP1(YDR007W)|FD-Score:6.83|P-value:4.15E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.57|P-value:2.56E-11||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.78|P-value:5.87E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.46|P-value:4.42E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:9.78|P-value:7.04E-23||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP6(YFR010W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VAC8(YEL013W)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VEL1(YGL258W)|FD-Score:6.02|P-value:8.74E-10||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:YBR062C(YBR062C_p)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR232C(YBR232C_d)|FD-Score:7.26|P-value:1.90E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR300C(YBR300C_d)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR087W(YCR087W_d)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.78|P-value:6.14E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Putative protein of unknown function Gene:YGR126W(YGR126W_p)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR130C(YGR130C)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHL008C(YHL008C_p)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIP5(YGL161C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YNR066C(YNR066C_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:ARO1(YDR127W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:8.75|P-value:1.03E-18||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASR1(YPR093C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG15(YCR068W)|FD-Score:4.76|P-value:9.64E-7||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATP17(YDR377W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BEM4(YPL161C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BRP1(YGL007W_d)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSC1(YDL037C)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BTS1(YPL069C)|FD-Score:5.79|P-value:3.51E-9||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CCW12(YLR110C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDA1(YLR307W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDC50(YCR094W)|FD-Score:6.22|P-value:2.44E-10||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COG5(YNL051W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS1(YNL336W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSG2(YBR036C)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DFG5(YMR238W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DOT6(YER088C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DRS2(YAL026C)|FD-Score:4.3|P-value:8.38E-6||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ENV10(YLR065C)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FET4(YMR319C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCN2(YDR283C)|FD-Score:8.85|P-value:4.27E-19||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:8.23|P-value:9.48E-17||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.93|P-value:2.05E-19||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCN5(YGR252W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:10.4|P-value:1.93E-25||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET2(YER083C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HSP82(YPL240C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HUG1(YML058W-A)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:7.81|P-value:2.79E-15||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:KAR4(YCL055W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KDX1(YKL161C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LOT5(YKL183W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MCK1(YNL307C)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MLH3(YPL164C)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRPS35(YGR165W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSB3(YNL293W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NCE102(YPR149W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:ORM2(YLR350W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PEX3(YDR329C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PEX6(YNL329C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PHO88(YBR106W)|FD-Score:7.43|P-value:5.33E-14||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PST1(YDR055W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTK2(YJR059W)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RAM1(YDL090C)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCE1(YMR274C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCY1(YJL204C)|FD-Score:11.8|P-value:2.18E-32||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGP1(YDR137W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL35B(YDL136W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX1(YBR228W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SLX9(YGR081C)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SOL1(YNR034W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPO23(YBR250W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:SPT23(YKL020C)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SWI4(YER111C)|FD-Score:5.78|P-value:3.84E-9||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYM1(YLR251W)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TOS6(YNL300W_p)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRP1(YDR007W)|FD-Score:6.83|P-value:4.15E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.57|P-value:2.56E-11||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.78|P-value:5.87E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.46|P-value:4.42E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:9.78|P-value:7.04E-23||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP6(YFR010W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:VAC8(YEL013W)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VEL1(YGL258W)|FD-Score:6.02|P-value:8.74E-10||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:YBR062C(YBR062C_p)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR232C(YBR232C_d)|FD-Score:7.26|P-value:1.90E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR300C(YBR300C_d)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCR049C(YCR049C_d)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR087W(YCR087W_d)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL241W(YDL241W_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.78|P-value:6.14E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Putative protein of unknown function Gene:YGR126W(YGR126W_p)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR130C(YGR130C)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YHL008C(YHL008C_p)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIP5(YGL161C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YNR066C(YNR066C_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative membrane-localized protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W23.101.42E-11810.60NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YMR134W_p12.601.84E-362.42ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YGR060W10.101.90E-240.38ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YHR007C9.759.03E-233.90ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YHR072W5.852.44E-90.45ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YOL146W5.403.29E-80.21PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YFL038C5.201.02E-70.29YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YJL086C_d4.904.71E-70.90YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YJL081C4.003.17E-50.20ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YCR093W3.807.09E-50.23CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YCR052W3.581.72E-40.39RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YNL272C3.197.02E-40.02SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
YJL143W3.177.57E-40.04TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YDR449C3.138.74E-40.04UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR064W3.099.86E-40.08RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C11.802.18E-32RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YDL226C10.401.93E-25GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YDR108W9.787.04E-23TRS85Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
YEL009C8.932.05E-19GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR283C8.854.27E-19GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDR035W8.751.03E-18ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YKR026C8.239.48E-17GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YKR019C7.812.79E-15IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDR354W7.464.42E-14TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YBR106W7.435.33E-14PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YBR232C_d7.261.90E-13YBR232C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR007W6.834.15E-12TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKL211C6.785.87E-12TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR008C_d6.786.14E-12YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER090W6.572.56E-11TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p

GO enrichment analysis for SGTC_834
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4810SGTC_20365160441 105.0 μMChembridge (Fragment library)1345340.08NEO1
0.4760SGTC_1797st048695 56.8 μMTimTec (Natural product derivative library)5922620.09375NEO1
0.4091.81E-236SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.125NEO1
0.4005.92E-226SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.125NEO1
0.3982.08E-223SGTC_7680384-0033 77.3 μMChemDiv (Drug-like library)39653050.0740741fatty acid desaturase (OLE1)
0.3981.02E-222SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.0769231NEO1
0.3752.95E-196SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0740741NEO1
0.3551.51E-174SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.134615
0.3538.92E-173SGTC_270tamoxifen 5.5 μMMiscellaneous53760.0892857NEO1
0.3512.11E-170SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.355556fatty acid desaturase (OLE1)
0.3492.61E-168SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0576923plasma membrane duress
0.3487.74E-168SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.131148plasma membrane duress
0.3427.55E-162SGTC_2725haloperidol 53.2 μMMiscellaneous35590.186441plasma membrane duress
0.3394.60E-159SGTC_12300170-0365 3.8 μMChemDiv (Drug-like library)40189320.0833333endomembrane recycling
0.3385.77E-158SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.192308fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1030844-010451.84 μM0.4255375804ChemDiv (Drug-like library)206.241062.10514ERAD & cell cycle
SGTC_3730844-010425.9 μM0.4255375804ChemDiv (Drug-like library)206.241062.10514ERAD & cell cycle
SGTC_750159-0059199.01 μM0.45377270ChemDiv (Drug-like library)251.238621.99916fatty acid desaturase (OLE1)
SGTC_22456637752200 μM0.394737681890Chembridge (Fragment library)199.63421.35112
SGTC_1025k007-073170 μM0.3809526256118ChemDiv (Drug-like library)272.2743233.26425
SGTC_5681193-00977.48 μM0.3571433646150ChemDiv (Drug-like library)242.658941.98724ERAD & cell cycle
SGTC_6371181-05197.26 μM0.3555566252091ChemDiv (Drug-like library)250.253860.70925fatty acid desaturase (OLE1)
SGTC_553k007-025645.7 μM0.3333336287000ChemDiv (Drug-like library)229.2528832.48414heme biosynthesis & mitochondrial translocase
SGTC_384k007-01445.16 μM0.3269236824886ChemDiv (Drug-like library)303.743561.91234ERAD & cell cycle
SGTC_7003453-2283142 μM0.326087565664ChemDiv (Drug-like library)263.719462.88912amide catabolism
SGTC_2872904403725.97 μM0.3255816465195Chembridge (Drug-like library)284.1131234.14912