0312-0010

(E)-3-(1-acetylindol-3-yl)prop-2-enal

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_835
Screen concentration 67.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 1626196
SMILES CC(=O)N1C=C(C2=CC=CC=C21)C=CC=O
Standardized SMILES CC(=O)n1cc(C=CC=O)c2ccccc12
Molecular weight 213.2319
ALogP 2.23
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.36
% growth inhibition (Hom. pool) 6.75


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1626196
Download HIP data (tab-delimited text)  (excel)
Gene:ERV1(YGR029W)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.61||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:GCD10(YNL062C)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GRS1(YBR121C)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.15||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:KRE5(YOR336W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.08||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NEO1(YIL048W)|FD-Score:-3.75|P-value:8.73E-5|Clearance:0||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PGA3(YML125C)|FD-Score:-3.97|P-value:3.54E-5|Clearance:0||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:UTP20(YBL004W)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YGR265W(YGR265W_d)|FD-Score:4.79|P-value:8.37E-7|Clearance:0.92||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:ZIM17(YNL310C)|FD-Score:-3.89|P-value:5.04E-5|Clearance:0||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix Gene:ERV1(YGR029W)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.61||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:GCD10(YNL062C)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GRS1(YBR121C)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.15||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:KRE5(YOR336W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.08||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:NEO1(YIL048W)|FD-Score:-3.75|P-value:8.73E-5|Clearance:0||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PGA3(YML125C)|FD-Score:-3.97|P-value:3.54E-5|Clearance:0||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:UTP20(YBL004W)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YGR265W(YGR265W_d)|FD-Score:4.79|P-value:8.37E-7|Clearance:0.92||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:ZIM17(YNL310C)|FD-Score:-3.89|P-value:5.04E-5|Clearance:0||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1626196
Download HOP data (tab-delimited text)  (excel)
Gene:ADE17(YMR120C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ARO7(YPR060C)|FD-Score:6.72|P-value:8.91E-12||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG7(YHR171W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BUD27(YFL023W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CHS5(YLR330W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CLB2(YPR119W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CPR7(YJR032W)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CYS4(YGR155W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:FRK1(YPL141C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUM1(YPL262W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCG1(YER163C_p)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GIR2(YDR152W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLN3(YER040W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GMC2(YLR445W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GPM3(YOL056W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GUP1(YGL084C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HOL1(YNR055C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:HPR1(YDR138W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HRB1(YNL004W)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:LRE1(YCL051W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSM1(YJL124C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MET28(YIR017C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MRM1(YOR201C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:NAP1(YKR048C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NBP2(YDR162C)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PHO8(YDR481C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PHO88(YBR106W)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMC1(YGL006W)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:ROT2(YBR229C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPN14(YGL004C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RRG9(YNL213C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SFP1(YLR403W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:TEF4(YKL081W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:UTH1(YKR042W)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS64(YDR200C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:WHI4(YDL224C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YAL065C(YAL065C_p)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YDL009C(YDL009C_p)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YHR202W(YHR202W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YLR126C(YLR126C_p)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function Gene:YNR014W(YNR014W_p)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YPL108W(YPL108W_p)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:ADE17(YMR120C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:ARO7(YPR060C)|FD-Score:6.72|P-value:8.91E-12||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG7(YHR171W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BUD27(YFL023W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CHS5(YLR330W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CLB2(YPR119W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CPR7(YJR032W)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CYS4(YGR155W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:FRK1(YPL141C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUM1(YPL262W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCG1(YER163C_p)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GIR2(YDR152W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLN3(YER040W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GMC2(YLR445W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GPM3(YOL056W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GUP1(YGL084C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HOL1(YNR055C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:HPR1(YDR138W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HRB1(YNL004W)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:LRE1(YCL051W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSM1(YJL124C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MET28(YIR017C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MRM1(YOR201C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:NAP1(YKR048C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NBP2(YDR162C)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PHO8(YDR481C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PHO88(YBR106W)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMC1(YGL006W)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:ROT2(YBR229C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPN14(YGL004C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RRG9(YNL213C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SFP1(YLR403W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:TEF4(YKL081W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:UTH1(YKR042W)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS64(YDR200C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:WHI4(YDL224C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YAL065C(YAL065C_p)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YDL009C(YDL009C_p)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YHR202W(YHR202W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YLR126C(YLR126C_p)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function Gene:YNR014W(YNR014W_p)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YPL108W(YPL108W_p)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR265W_d4.798.37E-70.92YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YGR029W3.875.49E-50.60ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YBR121C3.265.51E-40.15GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YOR336W3.119.28E-40.08KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YJL061W3.030.001210.00NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YLR086W3.030.001220.02SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YPR108W3.020.001280.02RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YDL208W3.000.001360.03NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YDR187C_d2.970.001480.05YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YJR123W2.920.001760.03RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YDR472W2.890.001930.04TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YBR136W2.850.002210.03MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YGL112C2.820.002420.17TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YKL018W2.640.004120.05SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YNL290W2.590.004760.01RFC3Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR060C6.728.91E-12ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YKR042W5.364.18E-8UTH1Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
YNR014W_p5.022.61E-7YNR014W_pPutative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication
YKR048C4.711.24E-6NAP1Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress
YER163C_p4.671.48E-6GCG1_pGamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
YDL224C4.651.70E-6WHI4Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
YDR162C4.513.17E-6NBP2Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
YHR202W_p4.241.10E-5YHR202W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
YDR200C4.171.50E-5VPS64Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
YGR155W3.885.15E-5CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YLR445W3.826.63E-5GMC2Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor
YER040W3.739.39E-5GLN3Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source
YKL081W3.701.08E-4TEF4Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
YIR017C3.691.10E-4MET28Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism
YJR032W3.661.29E-4CPR7Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity

GO enrichment analysis for SGTC_835
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1172.56E-19SGTC_30469092212 49.5 μMChembridge (Drug-like library)252366370.138462
0.0939.02E-13SGTC_9641287-0107 19.4 μMChemDiv (Drug-like library)515168120.105263cell wall
0.0822.20E-10SGTC_31019120531 49.5 μMChembridge (Drug-like library)248249040.084507
0.0741.09E-8SGTC_1656st012921 11.0 μMTimTec (Natural product derivative library)67328210.15
0.0715.42E-8SGTC_840443-0269 128.8 μMChemDiv (Drug-like library)61500580.145161
0.0681.68E-7SGTC_33039125618 40.6 μMChembridge (Drug-like library)226954670.0882353
0.0604.29E-6SGTC_9342950-0684 338.0 μMChemDiv (Drug-like library)11212530.138889
0.0604.53E-6SGTC_2791273-0055 2.9 μMChemDiv (Drug-like library)28475620.0491803azole & statin
0.0595.32E-6SGTC_990109-0120 622.8 μMChemDiv (Drug-like library)7594140.103448
0.0561.67E-5SGTC_29077721985 69.9 μMChembridge (Drug-like library)32678810.194444
0.0561.77E-5SGTC_1124fisetin 1.2 μMTimTec (Natural product library)52816140.0634921
0.0543.17E-5SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.112676
0.0543.32E-5SGTC_390dec-rvkr-cmk 16.7 μMMiscellaneous99620750.0470588calcium & mitochondrial duress
0.0534.91E-5SGTC_29579076800 11.5 μMChembridge (Drug-like library)164596150.0985916
0.0535.00E-5SGTC_2580diallyl disulphide 81.4 μMMicrosource (Natural product library)165900.0232558

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_10503232-115669.3 μM0.466667700529ChemDiv (Drug-like library)237.276523.0311RSC complex & mRNA processing
SGTC_12730849-009614.1 μM0.3673475286025ChemDiv (Drug-like library)216.235882.82302RPP1 & pyrimidine depletion
SGTC_1968st06959043.6 μM0.3148156384066TimTec (Natural product derivative library)229.254523.03102
SGTC_2552juarezic acid87.02 μM0.239131549512Microsource (Natural product library)174.19592.39512TSC3-RPN4
SGTC_7924534-1957155 μM0.2372881717904ChemDiv (Drug-like library)282.337022.73412
SGTC_8860866-0150153 μM0.235294271463ChemDiv (Drug-like library)199.248423.14201
SGTC_22597964009200 μM0.235294313134Chembridge (Fragment library)216.232582.7513superoxide
SGTC_1691st01993384.6 μM0.2307696176082TimTec (Natural product derivative library)236.308344.67401
SGTC_1776st04924945.9 μM0.2307691822314TimTec (Natural product derivative library)435.473944.73613
SGTC_2753fluvastatin17.09 μM0.228571446155Miscellaneous411.4659434.24435azole & statin