0330-0104

(Z)-4,4,4-trichloro-1-(4-nitrophenyl)but-2-en-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_836
Screen concentration 1.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 46494820
SMILES C1=CC(=CC=C1C(=O)C=CC(Cl)(Cl)Cl)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(cc1)C(=O)C=CC(Cl)(Cl)Cl
Molecular weight 294.5185
ALogP 3.61
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.4
% growth inhibition (Hom. pool) 9.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 46494820
Download HIP data (tab-delimited text)  (excel)
Gene:APC4(YDR118W)|FD-Score:-3.21|P-value:6.60E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:CBF2(YGR140W)|FD-Score:-4.96|P-value:3.59E-7|Clearance:0||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:GPI1(YGR216C)|FD-Score:-3.14|P-value:8.47E-4|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:MSL5(YLR116W)|FD-Score:3.35|P-value:4.10E-4|Clearance:0.3||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NOP56(YLR197W)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PMI40(YER003C)|FD-Score:5|P-value:2.82E-7|Clearance:0.75||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRP19(YLL036C)|FD-Score:5.4|P-value:3.29E-8|Clearance:0.75||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:PRP42(YDR235W)|FD-Score:3.35|P-value:4.07E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RPB10(YOR210W)|FD-Score:3.74|P-value:9.20E-5|Clearance:0.18||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB3(YIL021W)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.52||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC4(YFL005W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SPT14(YPL175W)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.21||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:TFC6(YDR362C)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.01||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YDR396W(YDR396W_d)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:APC4(YDR118W)|FD-Score:-3.21|P-value:6.60E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress Gene:CBF2(YGR140W)|FD-Score:-4.96|P-value:3.59E-7|Clearance:0||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:GPI1(YGR216C)|FD-Score:-3.14|P-value:8.47E-4|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:MSL5(YLR116W)|FD-Score:3.35|P-value:4.10E-4|Clearance:0.3||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NOP56(YLR197W)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PMI40(YER003C)|FD-Score:5|P-value:2.82E-7|Clearance:0.75||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRP19(YLL036C)|FD-Score:5.4|P-value:3.29E-8|Clearance:0.75||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:PRP42(YDR235W)|FD-Score:3.35|P-value:4.07E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RPB10(YOR210W)|FD-Score:3.74|P-value:9.20E-5|Clearance:0.18||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB3(YIL021W)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.52||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC4(YFL005W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SPT14(YPL175W)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.21||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:TFC6(YDR362C)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.01||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YDR396W(YDR396W_d)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 46494820
Download HOP data (tab-delimited text)  (excel)
Gene:AIM6(YDL237W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ARG80(YMR042W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:AZF1(YOR113W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BGL2(YGR282C)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BNA7(YDR428C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:CCE1(YKL011C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHS6(YJL099W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CIN2(YPL241C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CUS2(YNL286W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DBF2(YGR092W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DOT1(YDR440W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DSE4(YNR067C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ECM29(YHL030W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ELP2(YGR200C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EPT1(YHR123W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:FLR1(YBR008C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FYV1(YDR024W_d)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:HOS2(YGL194C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HOT13(YKL084W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:IXR1(YKL032C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LTV1(YKL143W)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MFM1(YPL060W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:PEP12(YOR036W)|FD-Score:7.21|P-value:2.80E-13||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:RAD51(YER095W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RIC1(YLR039C)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL19A(YBR084C-A)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SEC66(YBR171W)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIR1(YKR101W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SKN1(YGR143W)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SOL2(YCR073W-A)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:STR2(YJR130C)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:TMA17(YDL110C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TRP3(YKL211C)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:7.31|P-value:1.34E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS45(YGL095C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YET1(YKL065C)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YJL119C(YJL119C_d)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL030W(YKL030W_d)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLR012C(YLR012C_p)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR434C(YLR434C_d)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YNL013C(YNL013C_d)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL035C(YNL035C_p)|FD-Score:4.3|P-value:8.36E-6||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL226W(YNL226W_d)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNR065C(YNR065C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR331C(YOR331C_d)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL272C(YPL272C_p)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:AIM6(YDL237W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ARG80(YMR042W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:AZF1(YOR113W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BGL2(YGR282C)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BNA7(YDR428C)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:CCE1(YKL011C)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHS6(YJL099W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CIN2(YPL241C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CUS2(YNL286W)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DBF2(YGR092W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DOT1(YDR440W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DSE4(YNR067C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ECM29(YHL030W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ELP2(YGR200C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EPT1(YHR123W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:FLR1(YBR008C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FYV1(YDR024W_d)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:HOS2(YGL194C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HOT13(YKL084W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:IXR1(YKL032C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LTV1(YKL143W)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MFM1(YPL060W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:PEP12(YOR036W)|FD-Score:7.21|P-value:2.80E-13||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:RAD51(YER095W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RIC1(YLR039C)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL19A(YBR084C-A)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SEC66(YBR171W)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIR1(YKR101W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SKN1(YGR143W)|FD-Score:4.29|P-value:8.92E-6||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SOL2(YCR073W-A)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:STR2(YJR130C)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:TMA17(YDL110C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TRP3(YKL211C)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:7.31|P-value:1.34E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS45(YGL095C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YET1(YKL065C)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YJL119C(YJL119C_d)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL030W(YKL030W_d)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLR012C(YLR012C_p)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR434C(YLR434C_d)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YNL013C(YNL013C_d)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL035C(YNL035C_p)|FD-Score:4.3|P-value:8.36E-6||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL226W(YNL226W_d)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNR065C(YNR065C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR331C(YOR331C_d)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL272C(YPL272C_p)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLL036C5.403.29E-80.75PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YER003C5.002.82E-70.75PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YIL021W4.261.04E-50.52RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YOR210W3.749.20E-50.18RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YPL175W3.561.83E-40.21SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YDR362C3.363.94E-40.01TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YDR235W3.354.07E-40.00PRP42U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
YLR116W3.354.10E-40.30MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YFL029C3.040.001170.01CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YGR083C3.040.001190.10GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YDR164C2.940.001630.07SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
YGR147C2.880.002010.02NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YDR487C2.850.002160.06RIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
YMR208W2.800.002580.08ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YGR128C2.710.003330.01UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A7.311.34E-13TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOR036W7.212.80E-13PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YBR171W6.281.72E-10SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YKL143W5.991.07E-9LTV1Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature
YDR024W_d5.131.44E-7FYV1_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin
YNL226W_d5.101.69E-7YNL226W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
YGR282C4.651.68E-6BGL2Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance
YNL035C_p4.308.36E-6YNL035C_pProtein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress
YGR143W4.298.92E-6SKN1Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p
YPL272C_p4.191.39E-5YPL272C_pPutative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene
YHR123W4.141.77E-5EPT1sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
YDL136W4.052.55E-5RPL35BRibosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
YLR012C_p4.052.58E-5YLR012C_pPutative protein of unknown function; YLR012C is not an essential gene
YKL011C4.013.05E-5CCE1Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
YLR039C3.904.81E-5RIC1Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes

GO enrichment analysis for SGTC_836
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2351.18E-74SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.434783TSC3-RPN4
0.2061.69E-57SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.285714TSC3-RPN4
0.1951.15E-51SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.114286TSC3-RPN4
0.1871.51E-47SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0338983TSC3-RPN4
0.1843.12E-46SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.166667TSC3-RPN4
0.1797.93E-44SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.232143TSC3-RPN4
0.1791.31E-43SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0757576TSC3-RPN4
0.1756.29E-42SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.148148
0.1757.41E-42SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.109091TSC3-RPN4
0.1751.03E-41SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0857143
0.1682.07E-38SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.0555556
0.1646.32E-37SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.084507
0.1641.09E-36SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.163636TSC3-RPN4
0.1632.05E-36SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0517241TSC3-RPN4
0.1633.21E-36SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.078125TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1813st0512468.6 μM0.5641035291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_520329-0193875 nM0.4347833739292ChemDiv (Drug-like library)325.575642.80814TSC3-RPN4
SGTC_2350330-00875 mM0.42857146494818ChemDiv (Drug-like library)444.653284.76806TSC3-RPN4
SGTC_990109-0120622.8 μM0.386364759414ChemDiv (Drug-like library)321.126163.25713
SGTC_1828st05581149 μM0.3684215877589TimTec (Natural product derivative library)407.803265.69205
SGTC_5671080-054268.4 μM0.365950415ChemDiv (Drug-like library)296.277483.3115
SGTC_1967st07017751.4 μM0.355729214TimTec (Natural product derivative library)389.400665.14705
SGTC_12370330-013346.1 μM0.340426X1237ChemDiv (Drug-like library)308.548422.24342ergosterol biosynthesis
SGTC_12480469-068758.6 μM0.344017482ChemDiv (Drug-like library)284.223722.88805
SGTC_12600700-16177.75 μM0.3333332853078ChemDiv (Drug-like library)235.235922.80914calcium & mitochondrial duress