0109-0203

6-methyl-2-phenyl-1H-benzimidazole

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_838
Screen concentration 71.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 759422
SMILES CC1=CC2=C(C=C1)N=C(N2)C3=CC=CC=C3
Standardized SMILES Cc1ccc2[nH]c(nc2c1)c3ccccc3
Molecular weight 208.2585
ALogP 3.76
H-bond donor count 1
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.91
% growth inhibition (Hom. pool) 12.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 759422
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:-4.08|P-value:2.23E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CAB3(YKL088W)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.02||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:IPL1(YPL209C)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.05||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:NEO1(YIL048W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.07||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NSE1(YLR007W)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP159(YIL115C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.19||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:RPP1(YHR062C)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.21||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.8|P-value:7.24E-5|Clearance:0.43||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC2(YNL272C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.06||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SPP382(YLR424W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.01||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SPP41(YDR464W)|FD-Score:3.87|P-value:5.52E-5|Clearance:0.07||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:YPL238C(YPL238C_d)|FD-Score:7.31|P-value:1.35E-13|Clearance:3.24||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ARC40(YBR234C)|FD-Score:-4.08|P-value:2.23E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CAB3(YKL088W)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.02||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:IPL1(YPL209C)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.05||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:NEO1(YIL048W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.07||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NSE1(YLR007W)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP159(YIL115C)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.19||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:RPP1(YHR062C)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.21||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:3.8|P-value:7.24E-5|Clearance:0.43||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC2(YNL272C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.06||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SPP382(YLR424W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.01||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SPP41(YDR464W)|FD-Score:3.87|P-value:5.52E-5|Clearance:0.07||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:YPL238C(YPL238C_d)|FD-Score:7.31|P-value:1.35E-13|Clearance:3.24||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 759422
Download HOP data (tab-delimited text)  (excel)
Gene:AIM36(YMR157C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AQR1(YNL065W)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:COG8(YML071C)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM1(YCR086W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DBR1(YKL149C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DPH1(YIL103W)|FD-Score:5.95|P-value:1.31E-9||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM29(YHL030W)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ELP2(YGR200C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:GCN4(YEL009C)|FD-Score:-5.68|P-value:6.87E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GIM5(YML094W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HTA1(YDR225W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IRC7(YFR055W_p)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:IRS4(YKR019C)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MDM20(YOL076W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEF1(YLR069C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MEI4(YER044C-A)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MGM101(YJR144W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MOH1(YBL049W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPL4(YLR439W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL51(YPR100W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSN1(YOL116W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:OCA1(YNL099C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PHO87(YCR037C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:5.68|P-value:6.64E-9||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RBK1(YCR036W)|FD-Score:4.89|P-value:4.98E-7||SGD DESC:Putative ribokinase Gene:RGP1(YDR137W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL20B(YOR312C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:SEM1(YDR363W-A)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SLX5(YDL013W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF2(YOR290C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNZ2(YNL333W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPS100(YHR139C)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SYS1(YJL004C)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:THI2(YBR240C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRS85(YDR108W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VMA16(YHR026W)|FD-Score:4.64|P-value:1.73E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS63(YLR261C_d)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR203W(YDR203W_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL185C(YGL185C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR125W(YHR125W_d)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL007C(YJL007C_d)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL063C(YKL063C_p)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YLR111W(YLR111W_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR255C(YLR255C_d)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:AIM36(YMR157C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AQR1(YNL065W)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:COG8(YML071C)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM1(YCR086W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DBR1(YKL149C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DPH1(YIL103W)|FD-Score:5.95|P-value:1.31E-9||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM29(YHL030W)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ELP2(YGR200C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:GCN4(YEL009C)|FD-Score:-5.68|P-value:6.87E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GIM5(YML094W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HTA1(YDR225W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IRC7(YFR055W_p)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:IRS4(YKR019C)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MDM20(YOL076W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEF1(YLR069C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MEI4(YER044C-A)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MGM101(YJR144W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MOH1(YBL049W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPL4(YLR439W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL51(YPR100W)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSN1(YOL116W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:OCA1(YNL099C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PHO87(YCR037C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:3.09|P-value:9.92E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:5.68|P-value:6.64E-9||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RBK1(YCR036W)|FD-Score:4.89|P-value:4.98E-7||SGD DESC:Putative ribokinase Gene:RGP1(YDR137W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL20B(YOR312C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:SEM1(YDR363W-A)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SLX5(YDL013W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF2(YOR290C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNZ2(YNL333W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPS100(YHR139C)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SYS1(YJL004C)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:THI2(YBR240C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRS85(YDR108W)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VMA16(YHR026W)|FD-Score:4.64|P-value:1.73E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS63(YLR261C_d)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR203W(YDR203W_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL185C(YGL185C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR125W(YHR125W_d)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL007C(YJL007C_d)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL063C(YKL063C_p)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YLR111W(YLR111W_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR255C(YLR255C_d)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL238C_d7.311.35E-133.24YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YHR062C4.072.33E-50.20RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR464W3.875.52E-50.07SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YDR064W3.807.24E-50.43RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YIL048W3.373.74E-40.07NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YPL209C3.304.90E-40.05IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YLR424W3.255.78E-40.01SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YNL272C3.246.04E-40.06SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
YKL088W3.187.48E-40.02CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YIL115C3.167.98E-40.19NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YDL014W2.970.001510.10NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YIL026C2.870.002050.13IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YLR075W2.740.003100.01RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YBL092W2.730.003160.04RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YOL130W2.690.003580.00ALR1Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL103W5.951.31E-9DPH1Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
YNR052C5.686.64E-9POP2RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
YDR108W5.131.44E-7TRS85Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
YDR268W5.131.45E-7MSW1Mitochondrial tryptophanyl-tRNA synthetase
YCR036W4.894.98E-7RBK1Putative ribokinase
YHR026W4.641.73E-6VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YHR125W_d4.621.95E-6YHR125W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKR019C4.425.04E-6IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDR363W-A4.405.40E-6SEM1Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress
YJL007C_d4.405.48E-6YJL007C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YML071C4.092.11E-5COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR240C4.032.77E-5THI2Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type
YNL099C4.003.11E-5OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YDR203W_d3.944.04E-5YDR203W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL004C3.904.85E-5SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation

GO enrichment analysis for SGTC_838
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1301.25E-23SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.169231Golgi
0.1279.79E-23SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.113208Golgi
0.1249.36E-22SGTC_8490386-0493 241.0 μMChemDiv (Drug-like library)7261190.232558
0.1181.07E-19SGTC_389tpck 16.7 μMMiscellaneous4396470.172414
0.1172.17E-19SGTC_4982,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 150.0 μMICCB bioactive library23740.1Golgi
0.1158.72E-19SGTC_15324',5'-dihydroxyflavone 78.7 μMTimTec (Pure natural product library)1655210.0892857Golgi
0.1141.58E-18SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.122449Golgi
0.1128.65E-18SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.148936Golgi
0.1111.45E-17SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.245902Golgi
0.1087.74E-17SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.138462RPP1 & pyrimidine depletion
0.1062.26E-16SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.078125Golgi
0.1054.24E-16SGTC_8780828-0289 13.7 μMChemDiv (Drug-like library)7453500.189655
0.1041.22E-15SGTC_16014'-hydroxyflavanone 55.5 μMTimTec (Pure natural product library)1655060.0892857RPP1 & pyrimidine depletion
0.1023.59E-15SGTC_560957-0365 83.2 μMChemDiv (Drug-like library)67523600.15493Golgi
0.1023.86E-15SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.0862069

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7800109-0199192 μM0.5610414ChemDiv (Drug-like library)208.258483.75611
SGTC_660109-018742.4 μM0.473684759417ChemDiv (Drug-like library)228.676963.93511
SGTC_6990375-0592111 μM0.465116762578ChemDiv (Drug-like library)251.326283.71822iron homeostasis
SGTC_5382922-083853.3 μM0.44186777039ChemDiv (Drug-like library)259.305223.43222Golgi
SGTC_6200109-0052432 μM0.372549493037ChemDiv (Drug-like library)462.502583.34655
SGTC_13601598-002239.8 μM0.355556761525ChemDiv (Drug-like library)248.322345.07712ERG2
SGTC_3303912561840.58 μM0.34615422695467Chembridge (Drug-like library)332.422144.32714
SGTC_13151237-00193.69 μM0.340909825140ChemDiv (Drug-like library)246.26343.7303
SGTC_6271391-0720175 μM0.31746997839ChemDiv (Drug-like library)381.426565.60714
SGTC_20084012253172 μM0.317073603940Chembridge (Fragment library)185.225142.05513