0271-0008

2-phenylimino-1-benzothiophen-3-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_842
Screen concentration 11.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 1221709
SMILES C1=CC=C(C=C1)N=C2C(=O)C3=CC=CC=C3S2
Standardized SMILES O=C1C(=Nc2ccccc2)Sc3ccccc13
Molecular weight 239.2924
ALogP 3.71
H-bond donor count 0
H-bond acceptor count 3
Response signature heme biosynthesis & mitochondrial translocase

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.96
% growth inhibition (Hom. pool) 11.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1221709
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:5.33|P-value:4.84E-8|Clearance:0.49||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CAK1(YFL029C)|FD-Score:-3.78|P-value:7.80E-5|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:DBP2(YNL112W)|FD-Score:4.21|P-value:1.30E-5|Clearance:0.36||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG11(YHR007C)|FD-Score:-3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:EXO84(YBR102C)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.1||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GCD1(YOR260W)|FD-Score:3.2|P-value:6.96E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM1(YDR232W)|FD-Score:4.84|P-value:6.54E-7|Clearance:0.51||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HEM12(YDR047W)|FD-Score:12.8|P-value:8.36E-38|Clearance:2.88||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM2(YGL040C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.06||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HEM3(YDL205C)|FD-Score:13.6|P-value:2.25E-42|Clearance:2.88||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HYP2(YEL034W)|FD-Score:9.4|P-value:2.78E-21|Clearance:2.88||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MIA40(YKL195W)|FD-Score:6.12|P-value:4.60E-10|Clearance:0.79||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NOG1(YPL093W)|FD-Score:-3.13|P-value:8.83E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:OLE1(YGL055W)|FD-Score:-3.81|P-value:7.03E-5|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PAP1(YKR002W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.03||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PDC2(YDR081C)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PKC1(YBL105C)|FD-Score:3.84|P-value:6.05E-5|Clearance:0.15||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:10.2|P-value:8.25E-25|Clearance:2.88||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:-3.39|P-value:3.54E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPL25(YOL127W)|FD-Score:-3.12|P-value:9.03E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN3(YER021W)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSC58(YLR033W)|FD-Score:4.32|P-value:7.96E-6|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SFH1(YLR321C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SUB2(YDL084W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.1||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWD2(YKL018W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.04||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TIF34(YMR146C)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM17(YJL143W)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOA2(YKL058W)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.03||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:4.33|P-value:7.51E-6|Clearance:0.01||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTR5(YEL035C_p)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL196W(YDL196W_d)|FD-Score:6.52|P-value:3.52E-11|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL238C(YPL238C_d)|FD-Score:-3.25|P-value:5.87E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARP4(YJL081C)|FD-Score:5.33|P-value:4.84E-8|Clearance:0.49||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CAK1(YFL029C)|FD-Score:-3.78|P-value:7.80E-5|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:DBP2(YNL112W)|FD-Score:4.21|P-value:1.30E-5|Clearance:0.36||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG11(YHR007C)|FD-Score:-3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:EXO84(YBR102C)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.1||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GCD1(YOR260W)|FD-Score:3.2|P-value:6.96E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM1(YDR232W)|FD-Score:4.84|P-value:6.54E-7|Clearance:0.51||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HEM12(YDR047W)|FD-Score:12.8|P-value:8.36E-38|Clearance:2.88||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM2(YGL040C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.06||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HEM3(YDL205C)|FD-Score:13.6|P-value:2.25E-42|Clearance:2.88||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HYP2(YEL034W)|FD-Score:9.4|P-value:2.78E-21|Clearance:2.88||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MIA40(YKL195W)|FD-Score:6.12|P-value:4.60E-10|Clearance:0.79||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NOG1(YPL093W)|FD-Score:-3.13|P-value:8.83E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:OLE1(YGL055W)|FD-Score:-3.81|P-value:7.03E-5|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PAP1(YKR002W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.03||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PDC2(YDR081C)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PKC1(YBL105C)|FD-Score:3.84|P-value:6.05E-5|Clearance:0.15||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:10.2|P-value:8.25E-25|Clearance:2.88||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:-3.39|P-value:3.54E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPL25(YOL127W)|FD-Score:-3.12|P-value:9.03E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN3(YER021W)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSC58(YLR033W)|FD-Score:4.32|P-value:7.96E-6|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SFH1(YLR321C)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SUB2(YDL084W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.1||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWD2(YKL018W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.04||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TIF34(YMR146C)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM17(YJL143W)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOA2(YKL058W)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.03||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:4.33|P-value:7.51E-6|Clearance:0.01||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTR5(YEL035C_p)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL196W(YDL196W_d)|FD-Score:6.52|P-value:3.52E-11|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL238C(YPL238C_d)|FD-Score:-3.25|P-value:5.87E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1221709
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM37(YNL100W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALG12(YNR030W)|FD-Score:7.18|P-value:3.36E-13||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALT2(YDR111C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:API2(YDR525W_d)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARC18(YLR370C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG15(YCR068W)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BMH2(YDR099W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CIN4(YMR138W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COA4(YLR218C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:CYS4(YGR155W)|FD-Score:4.91|P-value:4.64E-7||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:FRE5(YOR384W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLR1(YPL091W)|FD-Score:16.2|P-value:2.00E-59||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:IES6(YEL044W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IOC2(YLR095C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRS4(YKR019C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:5.85|P-value:2.49E-9||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MGM1(YOR211C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MGR1(YCL044C)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MIC14(YDR031W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNN2(YBR015C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:NEM1(YHR004C)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:OPI3(YJR073C)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OST6(YML019W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:POP2(YNR052C)|FD-Score:-4.5|P-value:3.46E-6||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRO2(YOR323C)|FD-Score:5.29|P-value:6.26E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTK2(YJR059W)|FD-Score:-3.76|P-value:8.33E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP2(YOR208W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RHO4(YKR055W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPN4(YDL020C)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:8.73|P-value:1.27E-18||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFP1(YLR403W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLC1(YDL052C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SLT2(YHR030C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SRO9(YCL037C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STP1(YDR463W)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWF1(YDR126W)|FD-Score:21.6|P-value:8.17E-104||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TMA17(YDL110C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:UBP16(YPL072W)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:VAM7(YGL212W)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS34(YLR240W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YCR001W(YCR001W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:5.34|P-value:4.59E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YFH7(YFR007W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGR266W(YGR266W)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YIR021W-A(YIR021W-A_p)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLL044W(YLL044W_d)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YML012C-A(YML012C-A_d)|FD-Score:5.27|P-value:6.72E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR087W(YMR087W)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL184C(YNL184C_p)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR139C(YOR139C_d)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL162C(YPL162C_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL185W(YPL185W_d)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR064W(YPR064W_d)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR089W(YPR089W_p)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR197C(YPR197C_d)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSW1(YBR148W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YTA7(YGR270W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:AFT1(YGL071W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM37(YNL100W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALG12(YNR030W)|FD-Score:7.18|P-value:3.36E-13||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALT2(YDR111C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:API2(YDR525W_d)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARC18(YLR370C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG15(YCR068W)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BMH2(YDR099W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CIN4(YMR138W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COA4(YLR218C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:CYS4(YGR155W)|FD-Score:4.91|P-value:4.64E-7||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:FRE5(YOR384W)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLR1(YPL091W)|FD-Score:16.2|P-value:2.00E-59||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:IES6(YEL044W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IOC2(YLR095C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRS4(YKR019C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:5.85|P-value:2.49E-9||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MGM1(YOR211C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MGR1(YCL044C)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MIC14(YDR031W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNN2(YBR015C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:NEM1(YHR004C)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:OPI3(YJR073C)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OST6(YML019W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:POP2(YNR052C)|FD-Score:-4.5|P-value:3.46E-6||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRO2(YOR323C)|FD-Score:5.29|P-value:6.26E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTK2(YJR059W)|FD-Score:-3.76|P-value:8.33E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTP2(YOR208W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RHO4(YKR055W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPN4(YDL020C)|FD-Score:5.84|P-value:2.67E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:8.73|P-value:1.27E-18||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SFP1(YLR403W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLC1(YDL052C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SLT2(YHR030C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SRO9(YCL037C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STP1(YDR463W)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWF1(YDR126W)|FD-Score:21.6|P-value:8.17E-104||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TMA17(YDL110C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:UBP16(YPL072W)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:VAM7(YGL212W)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS34(YLR240W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YCR001W(YCR001W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:5.34|P-value:4.59E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YFH7(YFR007W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGR266W(YGR266W)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YIR021W-A(YIR021W-A_p)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLL044W(YLL044W_d)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YML012C-A(YML012C-A_d)|FD-Score:5.27|P-value:6.72E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR087W(YMR087W)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL184C(YNL184C_p)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR139C(YOR139C_d)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL162C(YPL162C_p)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL185W(YPL185W_d)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR064W(YPR064W_d)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR089W(YPR089W_p)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR197C(YPR197C_d)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSW1(YBR148W)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YTA7(YGR270W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL205C13.602.25E-422.88HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YDR047W12.808.36E-382.88HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YBR192W10.208.25E-252.88RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YEL034W9.402.78E-212.88HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YDL196W_d6.523.52E-110.40YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YKL195W6.124.60E-100.79MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YJL081C5.334.84E-80.49ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YDR232W4.846.54E-70.51HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YMR203W4.337.51E-60.01TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YLR033W4.327.96E-60.11RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YNL112W4.211.30E-50.36DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YBL105C3.846.05E-50.15PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YEL035C_p3.701.09E-40.05UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YBR102C3.651.31E-40.10EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YPR107C3.551.90E-40.02YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR126W21.608.17E-104SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YPL091W16.202.00E-59GLR1Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
YBR171W8.731.27E-18SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YNR030W7.183.36E-13ALG12Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
YAL024C5.852.49E-9LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YDL020C5.842.67E-9RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YCR064C_d5.344.59E-8YCR064C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
YOR323C5.296.26E-8PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YML012C-A_d5.276.72E-8YML012C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
YPL072W5.161.21E-7UBP16Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria
YGR155W4.914.64E-7CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YPR089W_p4.817.54E-7YPR089W_pProtein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p
YHR004C4.503.36E-6NEM1Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
YJR073C4.503.43E-6OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YPL185W_d4.493.59E-6YPL185W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C

GO enrichment analysis for SGTC_842
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3679.18E-188SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0483871heme biosynthesis & mitochondrial translocase
0.3004.15E-123SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.084507heme biosynthesis & mitochondrial translocase
0.2892.68E-114SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.18superoxide
0.2843.12E-110SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.192308heme biosynthesis & mitochondrial translocase
0.2796.75E-106SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.0983607SWF1 & branched chain AA biosynthesis
0.2743.70E-102SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.2superoxide
0.2601.55E-91SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.111111heme biosynthesis & mitochondrial translocase
0.2575.11E-90SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.142857heme biosynthesis & mitochondrial translocase
0.2464.48E-82SGTC_10441222-0021 1.8 μMChemDiv (Drug-like library)43067030.0645161heme biosynthesis & mitochondrial translocase
0.2444.64E-81SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0576923heme requiring
0.2361.50E-75SGTC_208k072-0098 12.4 μMChemDiv (Drug-like library)59489010.0925926heme biosynthesis & mitochondrial translocase
0.2356.96E-75SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.0483871heme biosynthesis & mitochondrial translocase
0.2358.81E-75SGTC_20345197015 53.1 μMChembridge (Fragment library)7806760.105263SWF1 & branched chain AA biosynthesis
0.2342.19E-74SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.0754717heme biosynthesis & mitochondrial translocase
0.2311.84E-72SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.107143heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1693xanthone101.9 μM0.2857147020TimTec (Natural product derivative library)196.201422.96402DNA damage response
SGTC_2532xanthone78.9 μM0.2857147020Microsource (Natural product library)196.201422.96402
SGTC_1069diphenylcyclopropenone8.49 μM0.27272765057NIH Clinical Collection206.23933.30101
SGTC_1915st06710530.6 μM0.2702769751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_2681menadione3.18 μM0.2631584055Miscellaneous172.180022.20402superoxide
SGTC_394menadione8.33 μM0.2631584055Miscellaneous172.180022.20402superoxide
SGTC_112phenothiazine287.22 μM0.2571437108ChemDiv (Drug-like library)199.271563.66812unfolded protein response
SGTC_90092-000210.45 μM0.255814759368ChemDiv (Drug-like library)276.286082.92603
SGTC_200481-00134.94 μM0.25182045ChemDiv (Drug-like library)241.070142.68602
SGTC_1169aniline yellow25.8 μM0.256051ChemDiv (Drug-like library)197.235843.45213
SGTC_14244048-1941275 μM0.244898X1424ChemDiv (Drug-like library)291.366924.0921
SGTC_290299-000412.98 μM0.24390216871ChemDiv (Drug-like library)188.179421.44503