0330-0132

4-(4-nitrophenyl)-6-(trichloromethyl)-5,6-dihydro-1,3-thiazin-2-imine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_844
Screen concentration 15.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 2828079
SMILES C1C(SC(=N)N=C1C2=CC=C(C=C2)[N+](=O)[O-])C(Cl)(Cl)Cl
Standardized SMILES [O-][N+](=O)c1ccc(cc1)C2=NC(=N)SC(C2)C(Cl)(Cl)Cl
Molecular weight 352.6241
ALogP 4.33
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.49
% growth inhibition (Hom. pool) 8.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2828079
Download HIP data (tab-delimited text)  (excel)
Gene:CDC1(YDR182W)|FD-Score:4.87|P-value:5.70E-7|Clearance:0.53||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:PGA3(YML125C)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.16||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:POL3(YDL102W)|FD-Score:5.29|P-value:6.02E-8|Clearance:0.43||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:RNT1(YMR239C)|FD-Score:-3.13|P-value:8.60E-4|Clearance:0||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RRP5(YMR229C)|FD-Score:4.34|P-value:7.27E-6|Clearance:0.81||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:SEC4(YFL005W)|FD-Score:7.14|P-value:4.57E-13|Clearance:1.85||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:YLR230W(YLR230W_d)|FD-Score:-3.45|P-value:2.83E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOR146W(YOR146W_d)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:CDC1(YDR182W)|FD-Score:4.87|P-value:5.70E-7|Clearance:0.53||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:PGA3(YML125C)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.16||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:POL3(YDL102W)|FD-Score:5.29|P-value:6.02E-8|Clearance:0.43||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:RNT1(YMR239C)|FD-Score:-3.13|P-value:8.60E-4|Clearance:0||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RRP5(YMR229C)|FD-Score:4.34|P-value:7.27E-6|Clearance:0.81||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:SEC4(YFL005W)|FD-Score:7.14|P-value:4.57E-13|Clearance:1.85||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:YLR230W(YLR230W_d)|FD-Score:-3.45|P-value:2.83E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOR146W(YOR146W_d)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2828079
Download HOP data (tab-delimited text)  (excel)
Gene:AAT1(YKL106W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:AIM9(YER080W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AKR1(YDR264C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALT2(YDR111C_p)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:ARO1(YDR127W)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.68|P-value:1.40E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASR1(YPR093C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:EMP47(YFL048C)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ERP5(YHR110W)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FUN30(YAL019W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GCN2(YDR283C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.22|P-value:1.05E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GLO2(YDR272W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GPG1(YGL121C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:KTR2(YKR061W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LDH1(YBR204C)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LRS4(YDR439W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAK3(YPR051W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:PAC2(YER007W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDR16(YNL231C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX17(YNL214W)|FD-Score:-3.74|P-value:9.32E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PEX31(YGR004W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFK26(YIL107C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:RAS1(YOR101W)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCE1(YMR274C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RHO5(YNL180C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPS7A(YOR096W)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RSR1(YGR152C)|FD-Score:3.59|P-value:1.69E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTF1(YGL244W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SHE2(YKL130C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SKG1(YKR100C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SOD1(YJR104C)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOL3(YHR163W)|FD-Score:-5.68|P-value:6.88E-9||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPS100(YHR139C)|FD-Score:-3.82|P-value:6.69E-5||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:TPO1(YLL028W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.3|P-value:5.76E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.03|P-value:5.07E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.37|P-value:3.96E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VMS1(YDR049W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS71(YML041C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR016W(YBR016W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDL218W(YDL218W_p)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR008C(YDR008C_d)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL218W(YJL218W_p)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YMR084W(YMR084W_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YPL162C(YPL162C_p)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPT53(YNL093W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:AAT1(YKL106W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:AIM9(YER080W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AKR1(YDR264C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALT2(YDR111C_p)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:ARO1(YDR127W)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.68|P-value:1.40E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASR1(YPR093C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:EMP47(YFL048C)|FD-Score:3.75|P-value:8.90E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ERP5(YHR110W)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FUN30(YAL019W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GCN2(YDR283C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.22|P-value:1.05E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GLO2(YDR272W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GPG1(YGL121C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:KTR2(YKR061W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LDH1(YBR204C)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LRS4(YDR439W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAK3(YPR051W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:PAC2(YER007W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDR16(YNL231C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX17(YNL214W)|FD-Score:-3.74|P-value:9.32E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PEX31(YGR004W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFK26(YIL107C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:RAS1(YOR101W)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCE1(YMR274C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RHO5(YNL180C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPS7A(YOR096W)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RSR1(YGR152C)|FD-Score:3.59|P-value:1.69E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:RTF1(YGL244W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SHE2(YKL130C)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SKG1(YKR100C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SOD1(YJR104C)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOL3(YHR163W)|FD-Score:-5.68|P-value:6.88E-9||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPS100(YHR139C)|FD-Score:-3.82|P-value:6.69E-5||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:TPO1(YLL028W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:5.97|P-value:1.20E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.3|P-value:5.76E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.03|P-value:5.07E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.37|P-value:3.96E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VMS1(YDR049W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS71(YML041C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR016W(YBR016W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDL218W(YDL218W_p)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin Gene:YDR008C(YDR008C_d)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL218W(YJL218W_p)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YMR084W(YMR084W_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YPL162C(YPL162C_p)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPT53(YNL093W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFL005W7.144.57E-131.85SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YDL102W5.296.02E-80.43POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YDR182W4.875.70E-70.53CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YMR229C4.347.27E-60.81RRP5RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
YOR146W_d3.532.10E-40.29YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YML125C3.236.09E-40.16PGA3Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
YBR102C3.070.001050.06EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YCL052C3.020.001280.07PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YGL099W2.940.001630.03LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YOL149W2.910.001810.02DCP1Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
YLL035W2.890.001918.34E-4GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YHR143W-A2.890.001920.28RPC10RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
YDR187C_d2.610.004530.05YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YDR487C2.550.005310.03RIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
YJL090C2.530.005780.00DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL009C8.221.05E-16GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YKL211C8.035.07E-16TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR007W5.971.20E-9TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR354W5.373.96E-8TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YER090W5.305.76E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR283C5.111.59E-7GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDR008C_d4.992.95E-7YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR184W4.963.61E-7GDB1Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
YDR035W4.681.40E-6ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YJL218W_p4.483.72E-6YJL218W_pPutative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene
YJR104C4.366.39E-6SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YER007W4.151.67E-5PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YNL093W3.973.58E-5YPT53Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
YBR016W3.953.93E-5YBR016WTail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
YKR061W3.944.10E-5KTR2Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_844
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4191.36E-249SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.084507
0.3904.96E-213SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.084507
0.3891.56E-212SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0166667
0.3851.66E-207SGTC_18825615643 20.0 μMMiscellaneous22530750.0722892TRP & mitochondrial translation
0.3816.48E-203SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0609756
0.3801.20E-201SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0882353
0.3756.42E-197SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0722892
0.3704.04E-191SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0746269
0.3632.24E-183SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0958904
0.3622.29E-182SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.0666667
0.3602.57E-180SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0454545
0.3592.10E-179SGTC_24885268135 45.7 μMMiscellaneous12703560.0757576
0.3593.09E-179SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.046875
0.3551.06E-174SGTC_18815652484 16.0 μMMiscellaneous22551290.060241TRP & mitochondrial translation
0.3512.76E-170SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0547945

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12370330-013346.1 μM0.384615X1237ChemDiv (Drug-like library)308.548422.24342ergosterol biosynthesis
SGTC_8360330-01041.35 μM0.30188746494820ChemDiv (Drug-like library)294.518543.6103
SGTC_1820st05376518.6 μM0.2916673690829TimTec (Natural product derivative library)428.459845.4106RPP1 & pyrimidine depletion
SGTC_520329-0193875 nM0.2711863739292ChemDiv (Drug-like library)325.575642.80814TSC3-RPN4
SGTC_11403229-18482.98 μM0.2678572849525ChemDiv (Drug-like library)254.285542.62105
SGTC_7791360-0071233 μM0.267566ChemDiv (Drug-like library)246.2853.09614fatty acid desaturase (OLE1)
SGTC_12600700-16177.75 μM0.2545452853078ChemDiv (Drug-like library)235.235922.80914calcium & mitochondrial duress
SGTC_2350330-00875 mM0.25396846494818ChemDiv (Drug-like library)444.653284.76806TSC3-RPN4
SGTC_1903970-07903.72 μM0.2456142832352ChemDiv (Drug-like library)286.23960.73805
SGTC_1360097-0015404.48 μM0.2407414861337ChemDiv (Drug-like library)494.448642.62701160S ribosome export