0433-0016

3-[5,6-dichloro-2-[3-[5,6-dichloro-1-ethyl-3-(3-sulfonatopropyl)benzimidazol-3-ium-2-yl]prop-2-enylidene]-3-ethylbenzimidazol-1-yl]propane-1-sulfonate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_856
Screen concentration 11.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 3911780
SMILES CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CCCS(=O)(=O)[O-])Cl)Cl)CC)CCCS(=O)(=O)[O-])Cl)Cl
Standardized SMILES CCN1C(=CC=Cc2[n+](CCCS(=O)(=O)[O-])c3cc(Cl)c(Cl)cc3n2CC)N(CCCS(=O)(=O)[O-])c4cc(Cl)c(Cl)cc14
Molecular weight 711.4844
ALogP 2.93
H-bond donor count 0
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.74
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3911780
Download HIP data (tab-delimited text)  (excel)
Gene:CDC21(YOR074C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.11||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:EMG1(YLR186W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.13||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:LUC7(YDL087C)|FD-Score:3.73|P-value:9.51E-5|Clearance:0.1||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MTR2(YKL186C)|FD-Score:4.96|P-value:3.53E-7|Clearance:1.23||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NOP1(YDL014W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.06||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:POP8(YBL018C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RDS3(YPR094W)|FD-Score:5.96|P-value:1.25E-9|Clearance:1.23||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RPS31(YLR167W)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.06||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RVB2(YPL235W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.08||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SPB1(YCL054W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.06||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:TAF9(YMR236W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.05||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:CDC21(YOR074C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.11||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:EMG1(YLR186W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.13||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:LUC7(YDL087C)|FD-Score:3.73|P-value:9.51E-5|Clearance:0.1||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MTR2(YKL186C)|FD-Score:4.96|P-value:3.53E-7|Clearance:1.23||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NOP1(YDL014W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.06||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:POP8(YBL018C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RDS3(YPR094W)|FD-Score:5.96|P-value:1.25E-9|Clearance:1.23||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RPS31(YLR167W)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.06||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RVB2(YPL235W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.08||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SPB1(YCL054W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.06||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:TAF9(YMR236W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.05||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3911780
Download HOP data (tab-delimited text)  (excel)
Gene:ADE3(YGR204W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM19(YIL087C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ASE1(YOR058C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:CBT1(YKL208W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CIT3(YPR001W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:EAF5(YEL018W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:EFG1(YGR271C-A)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELG1(YOR144C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMP24(YGL200C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:EPS1(YIL005W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FET4(YMR319C)|FD-Score:4.28|P-value:9.16E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FYV12(YOR183W_p)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GUD1(YDL238C)|FD-Score:4.52|P-value:3.06E-6||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HCR1(YLR192C)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IES6(YEL044W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IWR1(YDL115C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JJJ2(YJL162C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:LIP2(YLR239C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAG1(YER142C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAL13(YGR288W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAL32(YBR299W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MMS22(YLR320W)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN2(YBR015C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MPM1(YJL066C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRT4(YKL009W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSW1(YDR268W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NHA1(YLR138W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:PAH1(YMR165C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR12(YPL058C)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PFD1(YJL179W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PFK2(YMR205C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMS1(YNL082W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:RAD27(YKL113C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAD9(YDR217C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:ROX3(YBL093C)|FD-Score:5.63|P-value:8.86E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL20B(YOR312C)|FD-Score:6|P-value:1.02E-9||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SEM1(YDR363W-A)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SFG1(YOR315W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SLH1(YGR271W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPS100(YHR139C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SUV3(YPL029W)|FD-Score:4.86|P-value:5.84E-7||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TRX3(YCR083W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:YBR053C(YBR053C_p)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YDR271C(YDR271C_d)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YEH2(YLR020C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YEL014C(YEL014C_d)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YML089C(YML089C_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL198C(YNL198C_d)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YSP3(YOR003W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ADE3(YGR204W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM19(YIL087C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ASE1(YOR058C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:CBT1(YKL208W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CIT3(YPR001W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:EAF5(YEL018W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:EFG1(YGR271C-A)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELG1(YOR144C)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMP24(YGL200C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:EPS1(YIL005W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FET4(YMR319C)|FD-Score:4.28|P-value:9.16E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FYV12(YOR183W_p)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GUD1(YDL238C)|FD-Score:4.52|P-value:3.06E-6||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HCR1(YLR192C)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IES6(YEL044W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IWR1(YDL115C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JJJ2(YJL162C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:LIP2(YLR239C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAG1(YER142C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAL13(YGR288W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAL32(YBR299W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MMS22(YLR320W)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN2(YBR015C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MPM1(YJL066C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRT4(YKL009W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSW1(YDR268W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NHA1(YLR138W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:PAH1(YMR165C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR12(YPL058C)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PFD1(YJL179W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PFK2(YMR205C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMS1(YNL082W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:RAD27(YKL113C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAD9(YDR217C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:ROX3(YBL093C)|FD-Score:5.63|P-value:8.86E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL20B(YOR312C)|FD-Score:6|P-value:1.02E-9||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SEM1(YDR363W-A)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SFG1(YOR315W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate Gene:SLH1(YGR271W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SPS100(YHR139C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SUV3(YPL029W)|FD-Score:4.86|P-value:5.84E-7||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TRX3(YCR083W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:YBR053C(YBR053C_p)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Putative protein of unknown function; induced by cell wall perturbation Gene:YDR271C(YDR271C_d)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YEH2(YLR020C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YEL014C(YEL014C_d)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YML089C(YML089C_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL198C(YNL198C_d)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YSP3(YOR003W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Putative precursor to the subtilisin-like protease III

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR094W5.961.25E-91.23RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YKL186C4.963.53E-71.23MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YDL087C3.739.51E-50.10LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YOR074C3.631.41E-40.11CDC21Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YCL054W3.522.14E-40.06SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YDL014W3.462.68E-40.06NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YMR236W3.403.33E-40.05TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YPL235W3.354.01E-40.08RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YLR186W3.275.31E-40.13EMG1Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
YBL018C3.158.28E-40.01POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR167W3.148.52E-40.06RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YBR055C3.070.001060.01PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YLR166C3.070.001080.14SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YER104W2.920.001720.07RTT105Protein with a role in regulation of Ty1 transposition
YLR359W2.860.002140.06ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR312C6.001.02E-9RPL20BRibosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
YBL093C5.638.86E-9ROX3Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
YOR183W_p5.422.94E-8FYV12_pProtein of unknown function, required for survival upon exposure to K1 killer toxin
YDR363W-A5.393.47E-8SEM1Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress
YLR320W4.992.96E-7MMS22Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
YPL029W4.865.84E-7SUV3ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron
YLR239C4.741.07E-6LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YDL238C4.523.06E-6GUD1Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
YOR058C4.473.94E-6ASE1Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress
YMR319C4.289.16E-6FET4Low-affinity Fe(II) transporter of the plasma membrane
YPR001W4.221.25E-5CIT3Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
YOR315W4.191.40E-5SFG1Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate
YER142C4.102.08E-5MAG13-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
YJL162C4.092.14E-5JJJ2Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein
YDL115C3.953.89E-5IWR1RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_856
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0904.83E-12SGTC_31699102970 49.5 μMChembridge (Drug-like library)170278390.103448RPP1 & pyrimidine depletion
0.0905.21E-12SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.052083360S ribosome export
0.0612.53E-6SGTC_1543azetidyl-2-carboxylic acid 198.0 μMTimTec (Pure natural product library)164860.0131579
0.0595.02E-6SGTC_250118-0223 35.7 μMChemDiv (Drug-like library)53336280.043956
0.0587.43E-6SGTC_1554st069299 38.7 μMTimTec (Pure natural product library)242079990.0430108
0.0589.03E-6SGTC_458nppb 133.0 μMICCB bioactive library45490.0736842
0.0571.03E-5SGTC_14544239-0054 42.6 μMChemDiv (Drug-like library)13407670.0882353
0.0552.45E-5SGTC_1230083-0120 66.7 μMChemDiv (Drug-like library)160017010.121212
0.0534.48E-5SGTC_470149-0135 24.2 μMChemDiv (Drug-like library)52391550.060241TSC3-RPN4
0.0518.41E-5SGTC_21685727979 200.0 μMChembridge (Fragment library)6952100.0595238
0.0501.15E-4SGTC_7820083-0095 30.5 μMChemDiv (Drug-like library)35917290.0891089
0.0501.28E-4SGTC_31589100007 49.5 μMChembridge (Drug-like library)192972330.0752688
0.0501.38E-4SGTC_14013807-4679 7.6 μMChemDiv (Drug-like library)32914560.0421053tubulin folding & SWR complex
0.0491.52E-4SGTC_12981079-1278 263.0 μMChemDiv (Drug-like library)28409500.0638298DNA damage response
0.0482.31E-4SGTC_30879117406 49.5 μMChembridge (Drug-like library)425460930.117647

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_760433-0009400.33 μM0.3736262841569ChemDiv (Drug-like library)499.579261.01506
SGTC_12470448-00435.84 μM0.3571433089013ChemDiv (Drug-like library)370.379547.99200ERG2
SGTC_9021120-00022.07 μM0.2446813280475ChemDiv (Drug-like library)455.013667.96501azole & statin
SGTC_13812324-006662.1 μM0.23595510885928ChemDiv (Drug-like library)402.293766.78503
SGTC_5471120-0019610 nM0.2315793676939ChemDiv (Drug-like library)455.013667.96501azole & statin
SGTC_3832324-03957.37 μM0.1888893146421ChemDiv (Drug-like library)361.5237.25302azole & statin
SGTC_23369001819198.65 μM0.1866672989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_396dithiazanine1.25 μM0.18478310578Miscellaneous518.476593.92904
SGTC_1000086-012849.59 μM0.16049493297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress
SGTC_5914299-0174154 μM0.1590915509088ChemDiv (Drug-like library)368.835183.27915