0438-0306

2,5,6,7,8-pentafluoro-3-methylnaphthalene-1,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_857
Screen concentration 1.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 4686136
SMILES CC1=C(C(=O)C2=C(C1=O)C(=C(C(=C2F)F)F)F)F
Standardized SMILES CC1=C(F)C(=O)c2c(F)c(F)c(F)c(F)c2C1=O
Molecular weight 262.1323
ALogP 2.94
H-bond donor count 0
H-bond acceptor count 7
Response signature copper-dependent oxidative stress

Pool Growth Kinetics
% growth inhibition (Het. pool) 23.24
% growth inhibition (Hom. pool) 6.9


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4686136
Download HIP data (tab-delimited text)  (excel)
Gene:CAK1(YFL029C)|FD-Score:7.77|P-value:3.80E-15|Clearance:3.43||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CWC2(YDL209C)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:CYR1(YJL005W)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.01||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:DED1(YOR204W)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:GLC7(YER133W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MET30(YIL046W)|FD-Score:3.88|P-value:5.21E-5|Clearance:0.32||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MIM1(YOL026C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.23||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NUP49(YGL172W)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.06||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PRP24(YMR268C)|FD-Score:4.35|P-value:6.92E-6|Clearance:0.33||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RAP1(YNL216W)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPN3(YER021W)|FD-Score:4.02|P-value:2.97E-5|Clearance:0.13||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:TIM44(YIL022W)|FD-Score:3.22|P-value:6.47E-4|Clearance:0.05||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:YJL195C(YJL195C_d)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJR023C(YJR023C_d)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.33||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:CAK1(YFL029C)|FD-Score:7.77|P-value:3.80E-15|Clearance:3.43||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CWC2(YDL209C)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:CYR1(YJL005W)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.01||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:DED1(YOR204W)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:GLC7(YER133W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MET30(YIL046W)|FD-Score:3.88|P-value:5.21E-5|Clearance:0.32||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MIM1(YOL026C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.23||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NUP49(YGL172W)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.06||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PRP24(YMR268C)|FD-Score:4.35|P-value:6.92E-6|Clearance:0.33||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RAP1(YNL216W)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPN3(YER021W)|FD-Score:4.02|P-value:2.97E-5|Clearance:0.13||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:TIM44(YIL022W)|FD-Score:3.22|P-value:6.47E-4|Clearance:0.05||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:YJL195C(YJL195C_d)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJR023C(YJR023C_d)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.33||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4686136
Download HOP data (tab-delimited text)  (excel)
Gene:ANP1(YEL036C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:BUD2(YKL092C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CCS1(YMR038C)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DBF2(YGR092W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DYN3(YMR299C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FCY21(YER060W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GCG1(YER163C_p)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:HUR1(YGL168W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IOC3(YFR013W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:5.81|P-value:3.07E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM12(YOL009C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MND2(YIR025W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MON2(YNL297C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NAT5(YOR253W)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NDE1(YMR145C)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:PMR1(YGL167C)|FD-Score:7.1|P-value:6.31E-13||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PPT2(YPL148C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PST2(YDR032C)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PUS5(YLR165C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAS1(YOR101W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RNR4(YGR180C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL12B(YDR418W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:SAT4(YCR008W)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SLC1(YDL052C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SLI1(YGR212W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOD1(YJR104C)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO16(YHR153C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SPS19(YNL202W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:UBC8(YEL012W)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:YBR062C(YBR062C_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YCP4(YCR004C)|FD-Score:4.56|P-value:2.56E-6||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR015C(YGR015C_p)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YHR097C(YHR097C_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIH1(YCR059C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL024C(YIL024C_p)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YKL097C(YKL097C_d)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YML047W-A(YML047W-A_d)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:4.45|P-value:4.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS6(YIR039C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT35(YHR105W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ANP1(YEL036C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:BUD2(YKL092C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CCS1(YMR038C)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DBF2(YGR092W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DYN3(YMR299C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:FCY21(YER060W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GCG1(YER163C_p)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:HUR1(YGL168W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IOC3(YFR013W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:MAC1(YMR021C)|FD-Score:5.81|P-value:3.07E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM12(YOL009C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MND2(YIR025W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MON2(YNL297C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NAT5(YOR253W)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NDE1(YMR145C)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:PMR1(YGL167C)|FD-Score:7.1|P-value:6.31E-13||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PPT2(YPL148C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PST2(YDR032C)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PUS5(YLR165C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAS1(YOR101W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RNR4(YGR180C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL12B(YDR418W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:SAT4(YCR008W)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SLC1(YDL052C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SLI1(YGR212W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOD1(YJR104C)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO16(YHR153C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SPS19(YNL202W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:UBC8(YEL012W)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:YBR062C(YBR062C_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YCP4(YCR004C)|FD-Score:4.56|P-value:2.56E-6||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR015C(YGR015C_p)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YHR097C(YHR097C_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIH1(YCR059C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL024C(YIL024C_p)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YKL097C(YKL097C_d)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YML047W-A(YML047W-A_d)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:4.45|P-value:4.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS6(YIR039C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT35(YHR105W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFL029C7.773.80E-153.43CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YMR268C4.356.92E-60.33PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YER021W4.022.97E-50.14RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YIL046W3.885.21E-50.32MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YJR023C_d3.561.86E-40.33YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YJL005W3.236.16E-40.01CYR1Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
YIL022W3.226.47E-40.05TIM44Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
YGL172W3.177.61E-40.06NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YOL026C3.119.29E-40.23MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YEL032W2.880.001960.05MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YKL173W2.840.002280.02SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YGR190C_d2.820.002440.06YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YNL110C2.760.002910.06NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YDR243C2.700.003460.02PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YJL203W2.680.003720.05PRP21Subunit of the SF3a splicing factor complex, required for spliceosome assembly

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL167C7.106.31E-13PMR1High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
YMR021C5.813.07E-9MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YJR104C4.671.49E-6SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YCR004C4.562.56E-6YCP4Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR038C4.533.01E-6CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YPR123C_d4.454.28E-6YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YDR032C4.385.95E-6PST2Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
YHR153C4.221.23E-5SPO16Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
YPR124W4.221.24E-5CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YGR092W4.201.32E-5DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YCR059C4.171.52E-5YIH1Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
YCR008W4.131.79E-5SAT4Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
YGR015C_p3.924.34E-5YGR015C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
YOR385W_p3.924.40E-5YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YMR145C3.787.82E-5NDE1Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain

GO enrichment analysis for SGTC_857
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2092.90E-59SGTC_1060092-0003 11.6 μMChemDiv (Drug-like library)10721480.111111copper-dependent oxidative stress
0.2004.29E-54SGTC_6260214-0009 33.0 μMChemDiv (Drug-like library)34378740.0384615copper-dependent oxidative stress
0.1995.09E-54SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.114286copper-dependent oxidative stress
0.1884.92E-48SGTC_20805212524 12.4 μMChembridge (Fragment library)2601830.131579copper-dependent oxidative stress
0.1837.67E-46SGTC_200481-0013 4.9 μMChemDiv (Drug-like library)1820450.105263
0.1822.70E-45SGTC_10004048-0884 8.7 μMChemDiv (Drug-like library)130400880.0535714copper-dependent oxidative stress
0.1811.49E-44SGTC_10014048-4355 44.4 μMChemDiv (Drug-like library)218242330.0333333copper-dependent oxidative stress
0.1735.97E-41SGTC_1000086-0128 49.6 μMChemDiv (Drug-like library)932970.0816327copper-dependent oxidative stress
0.1721.14E-40SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.137255copper-dependent oxidative stress
0.1623.65E-36SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.131579copper-dependent oxidative stress
0.1531.76E-32SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.0888889superoxide
0.1524.78E-32SGTC_6120302-0167 22.4 μMChemDiv (Drug-like library)2358210.116279copper-dependent oxidative stress
0.1526.83E-32SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.179487superoxide
0.1432.36E-28SGTC_27815567276 71.4 μMChembridge (Drug-like library)28532740.0925926
0.1352.04E-25SGTC_7543474-0146 171.0 μMChemDiv (Drug-like library)247620780.0821918copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_110438-03071.3 μM0.4583334011476ChemDiv (Drug-like library)299.0054133.31706
SGTC_1550gossypolone36.6 μM0.2142865478622TimTec (Pure natural product library)546.521444.956410
SGTC_20685226020147 μM0.1842111810509Chembridge (Fragment library)195.602441.82112
SGTC_1556plumbagin1.48 μM0.17948710205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_2575plumbagin510 nM0.17948710205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_2681menadione3.18 μM0.1764714055Miscellaneous172.180022.20402superoxide
SGTC_394menadione8.33 μM0.1764714055Miscellaneous172.180022.20402superoxide
SGTC_495decylubiquinone155 μM0.1752971ICCB bioactive library322.43915.06204copper-dependent oxidative stress
SGTC_1078idebenone36.1 μM0.1707323686Miscellaneous338.43853.83615Golgi
SGTC_20024023503131 μM0.1707324736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_1370438-033285.39 μM0.1666672063393ChemDiv (Drug-like library)512.3515066.619212ubiquinone biosynthesis & proteasome
SGTC_470aa-861153 μM0.1666671967ICCB bioactive library326.429345.75713sphingolipid biosynthesis & PDR1