0438-0351

4-[2,6-dinitro-4-(trifluoromethyl)phenoxy]benzonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_858
Screen concentration 2.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 2799119
SMILES C1=CC(=CC=C1C#N)OC2=C(C=C(C=C2[N+](=O)[O-])C(F)(F)F)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1cc(cc(c1Oc2ccc(cc2)C#N)[N+](=O)[O-])C(F)(F)F
Molecular weight 353.2098
ALogP 4
H-bond donor count 0
H-bond acceptor count 9
Response signature copper-dependent oxidative stress

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.2
% growth inhibition (Hom. pool) 5.71


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2799119
Download HIP data (tab-delimited text)  (excel)
Gene:DUT1(YBR252W)|FD-Score:3.74|P-value:9.37E-5|Clearance:0.01||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERG12(YMR208W)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.01||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FRS2(YFL022C)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.22||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GRS1(YBR121C)|FD-Score:-3.28|P-value:5.10E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HEM12(YDR047W)|FD-Score:5.68|P-value:6.89E-9|Clearance:0.91||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HRT1(YOL133W)|FD-Score:3.72|P-value:9.93E-5|Clearance:0.16||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:HYP2(YEL034W)|FD-Score:4.03|P-value:2.82E-5|Clearance:0.13||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MAK21(YDR060W)|FD-Score:3.2|P-value:6.94E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:PFS2(YNL317W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.02||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PKC1(YBL105C)|FD-Score:3.26|P-value:5.66E-4|Clearance:0.06||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PMI40(YER003C)|FD-Score:6.3|P-value:1.51E-10|Clearance:0.91||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPN3(YER021W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RRS1(YOR294W)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.12||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC6(YCR052W)|FD-Score:4.76|P-value:9.50E-7|Clearance:0.52||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:TFC6(YDR362C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.06||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOA2(YKL058W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.16||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRR1(YDR353W)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTR5(YEL035C_p)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.13||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:DUT1(YBR252W)|FD-Score:3.74|P-value:9.37E-5|Clearance:0.01||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ERG12(YMR208W)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.01||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FRS2(YFL022C)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.22||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GRS1(YBR121C)|FD-Score:-3.28|P-value:5.10E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HEM12(YDR047W)|FD-Score:5.68|P-value:6.89E-9|Clearance:0.91||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HRT1(YOL133W)|FD-Score:3.72|P-value:9.93E-5|Clearance:0.16||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:HYP2(YEL034W)|FD-Score:4.03|P-value:2.82E-5|Clearance:0.13||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MAK21(YDR060W)|FD-Score:3.2|P-value:6.94E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:PFS2(YNL317W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.02||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PKC1(YBL105C)|FD-Score:3.26|P-value:5.66E-4|Clearance:0.06||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PMI40(YER003C)|FD-Score:6.3|P-value:1.51E-10|Clearance:0.91||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RPN3(YER021W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RRS1(YOR294W)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.12||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC6(YCR052W)|FD-Score:4.76|P-value:9.50E-7|Clearance:0.52||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:TFC6(YDR362C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.06||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOA2(YKL058W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.16||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRR1(YDR353W)|FD-Score:-3.41|P-value:3.20E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTR5(YEL035C_p)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.13||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2799119
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM26(YKL037W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM45(YPR004C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ARA2(YMR041C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ASI1(YMR119W)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BGL2(YGR282C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CCS1(YMR038C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX14(YML129C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CTH1(YDR151C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:CYS3(YAL012W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DIE2(YGR227W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:EMC5(YIL027C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENT3(YJR125C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:GET2(YER083C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLY1(YEL046C)|FD-Score:8.41|P-value:2.08E-17||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GUF1(YLR289W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:GUP1(YGL084C)|FD-Score:6.04|P-value:7.55E-10||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HEH2(YDR458C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HOM2(YDR158W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HXT2(YMR011W)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ICY2(YPL250C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:MAF1(YDR005C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MEI4(YER044C-A)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MET22(YOL064C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MSD1(YPL104W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NAT1(YDL040C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NAT5(YOR253W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NBA1(YOL070C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:PEX34(YCL056C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHO88(YBR106W)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIB2(YGL023C)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PRO2(YOR323C)|FD-Score:7.94|P-value:1.01E-15||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSR1(YLL010C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PST1(YDR055W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:RAD17(YOR368W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD59(YDL059C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RCE1(YMR274C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:REG1(YDR028C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPL34B(YIL052C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIS2(YKR072C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SNF8(YPL002C)|FD-Score:4|P-value:3.15E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOD1(YJR104C)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STF2(YGR008C)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:SWC5(YBR231C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCM62(YBR044C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TPK2(YPL203W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP4(YDR354W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:12.9|P-value:3.40E-38||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR1(YGR184C)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VCX1(YDL128W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:VPS25(YJR102C)|FD-Score:5.36|P-value:4.13E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function Gene:YBL044W(YBL044W_p)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBR099C(YBR099C_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR232C(YBR232C_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR025C(YCR025C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDR095C(YDR095C_d)|FD-Score:-3.75|P-value:8.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YER087C-A(YER087C-A_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGR125W(YGR125W_p)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR130C(YGR130C)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YKL044W(YKL044W_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YNL122C(YNL122C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOL099C(YOL099C_d)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR123C(YPR123C_d)|FD-Score:5.87|P-value:2.13E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:AFT1(YGL071W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM26(YKL037W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM45(YPR004C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ARA2(YMR041C)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Gene:ASI1(YMR119W)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BGL2(YGR282C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CCS1(YMR038C)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX14(YML129C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CTH1(YDR151C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:CYS3(YAL012W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DIE2(YGR227W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:EMC5(YIL027C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENT3(YJR125C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:GET2(YER083C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLY1(YEL046C)|FD-Score:8.41|P-value:2.08E-17||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GUF1(YLR289W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:GUP1(YGL084C)|FD-Score:6.04|P-value:7.55E-10||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HEH2(YDR458C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HOM2(YDR158W)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HXT2(YMR011W)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ICY2(YPL250C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:MAF1(YDR005C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MEI4(YER044C-A)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MET22(YOL064C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MSD1(YPL104W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NAT1(YDL040C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NAT5(YOR253W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NBA1(YOL070C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:PEX34(YCL056C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHO88(YBR106W)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIB2(YGL023C)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PRO2(YOR323C)|FD-Score:7.94|P-value:1.01E-15||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSR1(YLL010C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PST1(YDR055W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:RAD17(YOR368W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD59(YDL059C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RCE1(YMR274C)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:REG1(YDR028C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPL34B(YIL052C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIS2(YKR072C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SNF8(YPL002C)|FD-Score:4|P-value:3.15E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOD1(YJR104C)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STF2(YGR008C)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress Gene:SWC5(YBR231C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCM62(YBR044C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TPK2(YPL203W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP4(YDR354W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:12.9|P-value:3.40E-38||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR1(YGR184C)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VCX1(YDL128W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:VPS25(YJR102C)|FD-Score:5.36|P-value:4.13E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function Gene:YBL044W(YBL044W_p)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBR099C(YBR099C_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YBR232C(YBR232C_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR025C(YCR025C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDR095C(YDR095C_d)|FD-Score:-3.75|P-value:8.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YER087C-A(YER087C-A_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGR125W(YGR125W_p)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR130C(YGR130C)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YKL044W(YKL044W_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YNL122C(YNL122C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOL099C(YOL099C_d)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR123C(YPR123C_d)|FD-Score:5.87|P-value:2.13E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER003C6.301.51E-100.91PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YDR047W5.686.89E-90.91HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YCR052W4.769.50E-70.52RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YFL022C4.251.09E-50.22FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YEL034W4.032.82E-50.13HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YOR294W3.904.79E-50.12RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YMR208W3.787.95E-50.04ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YBR252W3.749.37E-50.01DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YOL133W3.729.93E-50.16HRT1RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF
YDR362C3.561.84E-40.06TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YNL317W3.502.33E-40.02PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YER021W3.482.48E-40.05RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YJL085W3.433.03E-40.01EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YKL058W3.423.14E-40.16TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YBL105C3.265.66E-40.06PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A12.903.40E-38TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YEL046C8.412.08E-17GLY1Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
YOR323C7.941.01E-15PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YGL084C6.047.55E-10GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YPR123C_d5.872.13E-9YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YJR102C5.364.13E-8VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YJR104C4.973.33E-7SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YPL104W4.741.09E-6MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
YLR289W4.711.25E-6GUF1Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
YGR184C4.562.61E-6UBR1E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)
YGR130C4.523.07E-6YGR130CComponent of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
YOL099C_d4.473.86E-6YOL099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes
YMR119W4.385.92E-6ASI1Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
YBR106W4.279.69E-6PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YNR025C_d4.241.11E-5YNR025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase

GO enrichment analysis for SGTC_858
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3003.46E-123SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0625TSC3-RPN4
0.2982.43E-121SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0645161TSC3-RPN4
0.2781.48E-105SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0967742TSC3-RPN4
0.2654.46E-95SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0727273
0.2647.53E-95SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0684932TSC3-RPN4
0.2611.67E-92SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0769231TSC3-RPN4
0.2603.78E-92SGTC_10014048-4355 44.4 μMChemDiv (Drug-like library)218242330.126761copper-dependent oxidative stress
0.2531.01E-86SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.15873TSC3-RPN4
0.2481.50E-83SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.09375TSC3-RPN4
0.2471.55E-82SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.151515TSC3-RPN4
0.2445.60E-81SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0864198TSC3-RPN4
0.2354.39E-75SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0793651TSC3-RPN4
0.2331.71E-73SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.114754TSC3-RPN4
0.2324.24E-73SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0779221
0.2271.11E-69SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0491803

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9821491-051827.5 μM0.3448283091256ChemDiv (Drug-like library)416.265653.336211
SGTC_7680384-003377.3 μM0.3269233965305ChemDiv (Drug-like library)275.216962.43216fatty acid desaturase (OLE1)
SGTC_480382-001855 μM0.288462762596ChemDiv (Drug-like library)231.204163.04214
SGTC_13581509-00222.48 μM0.2727273258434ChemDiv (Drug-like library)340.204162.48408
SGTC_9691313-0238134 μM0.255212874ChemDiv (Drug-like library)352.264753.37117mitochondrial processes
SGTC_1663st01414055.4 μM0.2463774207120TimTec (Natural product derivative library)361.307742.311760S ribosome export
SGTC_2361509-00115.31 μM0.2456143107280ChemDiv (Drug-like library)295.20662.5906
SGTC_3861509-00113.07 μM0.2456143107280ChemDiv (Drug-like library)295.20662.5906
SGTC_1725st03628420.55 μM0.241379707914TimTec (Natural product derivative library)243.17511.67806
SGTC_16erodoxin22.81 μM0.2352943967339ChemDiv (Drug-like library)277.0292.3505unfolded protein response
SGTC_14974476-297841.3 μM0.2333331528379ChemDiv (Drug-like library)331.310193.24317