0469-0573

4,5,6-trichloro-3H-1,3-benzoxazole-2-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_864
Screen concentration 172.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 689425
SMILES C1=C2C(=C(C(=C1Cl)Cl)Cl)NC(=S)O2
Standardized SMILES Sc1oc2cc(Cl)c(Cl)c(Cl)c2n1
Molecular weight 254.5209
ALogP 4.7
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.54
% growth inhibition (Hom. pool) 2.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 689425
Download HIP data (tab-delimited text)  (excel)
Gene:MCM10(YIL150C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MOT1(YPL082C)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.09||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NMT1(YLR195C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.24||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NOP14(YDL148C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.21||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRE2(YPR103W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.28||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RER2(YBR002C)|FD-Score:3.95|P-value:3.92E-5|Clearance:0.28||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC4(YOL094C)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.09||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIO1(YOR119C)|FD-Score:-5.05|P-value:2.18E-7|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:SRP54(YPR088C)|FD-Score:-6.63|P-value:1.64E-11|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TIF34(YMR146C)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.28||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:VRG4(YGL225W)|FD-Score:-3.18|P-value:7.25E-4|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDL196W(YDL196W_d)|FD-Score:-3.82|P-value:6.65E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YIP1(YGR172C)|FD-Score:4.31|P-value:8.02E-6|Clearance:0.28||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:YPL142C(YPL142C_d)|FD-Score:4.38|P-value:5.82E-6|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:MCM10(YIL150C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MOT1(YPL082C)|FD-Score:3.23|P-value:6.23E-4|Clearance:0.09||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NMT1(YLR195C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.24||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NOP14(YDL148C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.21||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRE2(YPR103W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.28||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RER2(YBR002C)|FD-Score:3.95|P-value:3.92E-5|Clearance:0.28||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFC4(YOL094C)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.09||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIO1(YOR119C)|FD-Score:-5.05|P-value:2.18E-7|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:SRP54(YPR088C)|FD-Score:-6.63|P-value:1.64E-11|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TIF34(YMR146C)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.28||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:VRG4(YGL225W)|FD-Score:-3.18|P-value:7.25E-4|Clearance:0||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDL196W(YDL196W_d)|FD-Score:-3.82|P-value:6.65E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YIP1(YGR172C)|FD-Score:4.31|P-value:8.02E-6|Clearance:0.28||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:YPL142C(YPL142C_d)|FD-Score:4.38|P-value:5.82E-6|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 689425
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG36(YJL185C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:BUB3(YOR026W)|FD-Score:-3.75|P-value:8.77E-5||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:BUD31(YCR063W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:BZZ1(YHR114W)|FD-Score:4.27|P-value:9.80E-6||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:COX12(YLR038C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CRD1(YDL142C)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CTA1(YDR256C)|FD-Score:-3.21|P-value:6.52E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:FUI1(YBL042C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GTT3(YEL017W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HSP42(YDR171W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HXT12(YIL170W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:ISC10(YER180C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:JJJ2(YJL162C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:LIP5(YOR196C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LST7(YGR057C)|FD-Score:6.06|P-value:6.63E-10||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MBR1(YKL093W)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MEK1(YOR351C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MET32(YDR253C)|FD-Score:-4.31|P-value:8.01E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MET5(YJR137C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MPH1(YIR002C)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPL17(YNL252C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS5(YBR251W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC6(YHR151C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NCA3(YJL116C)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NUM1(YDR150W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OM14(YBR230C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPT2(YPR194C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PEX14(YGL153W)|FD-Score:-4.62|P-value:1.93E-6||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PFS1(YHR185C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHR1(YOR386W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PMT6(YGR199W)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases Gene:PRM2(YIL037C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PSP2(YML017W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTH2(YBL057C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUS7(YOR243C)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD50(YNL250W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RKM1(YPL208W)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPL14A(YKL006W)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:4.81|P-value:7.50E-7||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:-3.9|P-value:4.77E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEC66(YBR171W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLI1(YGR212W)|FD-Score:6.52|P-value:3.63E-11||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SLM6(YBR266C_d)|FD-Score:3.77|P-value:8.00E-5||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNO2(YNL334C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SRY1(YKL218C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSK1(YLR006C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TPS3(YMR261C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TYW1(YPL207W)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:WSC2(YNL283C)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL053W(YBL053W_d)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR074W(YBR074W_p)|FD-Score:5.04|P-value:2.36E-7||SGD DESC:Putative metalloprotease Gene:YBR138C(YBR138C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDL032W(YDL032W_d)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR286C(YDR286C_p)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YGR026W(YGR026W_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR210C(YGR210C_p)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR022C-A(YHR022C-A_p)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHR180W(YHR180W_d)|FD-Score:-5.77|P-value:4.00E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL215C(YJL215C_d)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR079W(YJR079W_p)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKL136W(YKL136W_d)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function Gene:YML009C-A(YML009C-A_d)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL022C(YNL022C_p)|FD-Score:-3.85|P-value:5.85E-5||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNR029C(YNR029C_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR063W(YNR063W_p)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR342C(YOR342C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:-6.03|P-value:8.45E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL102C(YPL102C_d)|FD-Score:4.77|P-value:9.41E-7||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:AFT1(YGL071W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG36(YJL185C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:BUB3(YOR026W)|FD-Score:-3.75|P-value:8.77E-5||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:BUD31(YCR063W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:BZZ1(YHR114W)|FD-Score:4.27|P-value:9.80E-6||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:COX12(YLR038C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CRD1(YDL142C)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CTA1(YDR256C)|FD-Score:-3.21|P-value:6.52E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:FUI1(YBL042C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GTT3(YEL017W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HSP42(YDR171W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:HXT12(YIL170W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:ISC10(YER180C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:JJJ2(YJL162C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:LIP5(YOR196C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LST7(YGR057C)|FD-Score:6.06|P-value:6.63E-10||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MBR1(YKL093W)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MEK1(YOR351C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MET32(YDR253C)|FD-Score:-4.31|P-value:8.01E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MET5(YJR137C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MPH1(YIR002C)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPL17(YNL252C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS5(YBR251W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTC6(YHR151C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NCA3(YJL116C)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NUM1(YDR150W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OM14(YBR230C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPT2(YPR194C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PEX14(YGL153W)|FD-Score:-4.62|P-value:1.93E-6||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PFS1(YHR185C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHR1(YOR386W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PMT6(YGR199W)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases Gene:PRM2(YIL037C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PSP2(YML017W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTH2(YBL057C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUS7(YOR243C)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD50(YNL250W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RKM1(YPL208W)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPL14A(YKL006W)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:4.81|P-value:7.50E-7||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:-3.9|P-value:4.77E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEC66(YBR171W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLI1(YGR212W)|FD-Score:6.52|P-value:3.63E-11||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SLM6(YBR266C_d)|FD-Score:3.77|P-value:8.00E-5||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNO2(YNL334C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SRY1(YKL218C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSK1(YLR006C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TPS3(YMR261C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TYW1(YPL207W)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:WSC2(YNL283C)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL053W(YBL053W_d)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR074W(YBR074W_p)|FD-Score:5.04|P-value:2.36E-7||SGD DESC:Putative metalloprotease Gene:YBR138C(YBR138C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDL032W(YDL032W_d)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL119C(YDL119C_p)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR286C(YDR286C_p)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site Gene:YGR026W(YGR026W_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR210C(YGR210C_p)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR022C-A(YHR022C-A_p)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YHR180W(YHR180W_d)|FD-Score:-5.77|P-value:4.00E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL215C(YJL215C_d)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR079W(YJR079W_p)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKL136W(YKL136W_d)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function Gene:YML009C-A(YML009C-A_d)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL022C(YNL022C_p)|FD-Score:-3.85|P-value:5.85E-5||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNR029C(YNR029C_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR063W(YNR063W_p)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR342C(YOR342C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:-6.03|P-value:8.45E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL102C(YPL102C_d)|FD-Score:4.77|P-value:9.41E-7||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL142C_d4.385.82E-60.28YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YGR172C4.318.02E-60.28YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YMR146C4.121.91E-50.28TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YPR103W4.102.11E-50.28PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YBR002C3.953.92E-50.28RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YLR195C3.671.19E-40.24NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YDL148C3.442.94E-40.21NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YPL082C3.236.23E-40.09MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YIL150C3.148.53E-40.00MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YOL094C3.148.58E-40.09RFC4Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YDR413C_d3.050.001150.00YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YBR136W3.040.001160.17MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YMR047C2.880.002010.05NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
YDL015C2.820.002370.00TSC13Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
YDL030W2.820.002400.03PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR212W6.523.63E-11SLI1N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
YER090W6.212.67E-10TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YGR057C6.066.63E-10LST7Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
YBR074W_p5.042.36E-7YBR074W_pPutative metalloprotease
YML063W4.817.50E-7RPS1BRibosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication
YPL102C_d4.779.41E-7YPL102C_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4
YJL162C4.721.20E-6JJJ2Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein
YML009C-A_d4.661.56E-6YML009C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBL053W_d4.572.42E-6YBL053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNL334C4.503.47E-6SNO2Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
YHR114W4.279.80E-6BZZ1SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins
YDL032W_d4.231.15E-5YDL032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene
YLR006C4.121.93E-5SSK1Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
YHR185C4.092.20E-5PFS1Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation
YJL185C4.082.27E-5ATG36Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene

GO enrichment analysis for SGTC_864
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1424.10E-28SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.115385TSC3-RPN4
0.1287.55E-23SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)5150859NA
0.1125.22E-18SGTC_2231micatex 6.9 μMMiscellaneous207370.0731707
0.1023.17E-15SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.0701754
0.1024.50E-15SGTC_22967971656 126.5 μMChembridge (Fragment library)6665700.0793651
0.0992.58E-14SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.047619
0.0978.40E-14SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.0384615
0.0952.07E-13SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.101695
0.0952.28E-13SGTC_20645220758 200.0 μMChembridge (Fragment library)7885180.0333333
0.0945.39E-13SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.06
0.0921.16E-12SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0909091
0.0911.94E-12SGTC_14554262-0120 131.0 μMChemDiv (Drug-like library)15295870.0425532plasma membrane duress
0.0872.33E-11SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.05
0.0872.47E-11SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.030303TSC3-RPN4
0.0856.68E-11SGTC_1876myriocin 605.0 nMMiscellaneous301119NA

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_100279-021242.58 μM0.61290339851ChemDiv (Drug-like library)238.455283.33312TRP & mitochondrial translation
SGTC_190279-021438.43 μM0.3513513566274ChemDiv (Drug-like library)237.447342.94503TRP & mitochondrial translation
SGTC_2398522821348.84 μM0.22580771Miscellaneous216.020921.99912iron homeostasis
SGTC_23276189656200 μM0.2083335349088Chembridge (Fragment library)282.702882.18634
SGTC_20315180455200 μM0.19354829949Chembridge (Fragment library)177.031241.66522
SGTC_13561502-078185.8 μM0.192308567702ChemDiv (Drug-like library)284.761823.53804fatty acid desaturase (OLE1)
SGTC_23579067248200 μM0.1914899150235Chembridge (Fragment library)225.628421.18413
SGTC_1459k035-003135.7 μM0.1860473877128ChemDiv (Drug-like library)260.471922.08312DNA intercalators
SGTC_20685226020147 μM0.1860471810509Chembridge (Fragment library)195.602441.82112
SGTC_1526st05151875 μM0.185185673993TimTec (Pure natural product library)266.677043.44504