0488-0499

3-N-[(E)-benzylideneamino]-1,2,5-oxadiazole-3,4-diamine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_865
Screen concentration 301.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 9634651
SMILES C1=CC=C(C=C1)C=NNC2=NON=C2N
Standardized SMILES Nc1nonc1NN=Cc2ccccc2
Molecular weight 203.2007
ALogP 1.58
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.78
% growth inhibition (Hom. pool) 5.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9634651
Download HIP data (tab-delimited text)  (excel)
Gene:BET2(YPR176C)|FD-Score:3.84|P-value:6.12E-5|Clearance:0.33||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:DBP6(YNR038W)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.33||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:GPI11(YDR302W)|FD-Score:-3.41|P-value:3.24E-4|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:NUF2(YOL069W)|FD-Score:3.73|P-value:9.67E-5|Clearance:0.33||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUT2(YPR168W)|FD-Score:3.14|P-value:8.55E-4|Clearance:0.07||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:ORC4(YPR162C)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.33||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RFA1(YAR007C)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RPC25(YKL144C)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPS13(YDR064W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SNP1(YIL061C)|FD-Score:3.25|P-value:5.79E-4|Clearance:0.11||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:UBA1(YKL210W)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.12||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:ULP1(YPL020C)|FD-Score:-3.44|P-value:2.93E-4|Clearance:0||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:YGR114C(YGR114C_d)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YPL238C(YPL238C_d)|FD-Score:3.28|P-value:5.17E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR177C(YPR177C_d)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:BET2(YPR176C)|FD-Score:3.84|P-value:6.12E-5|Clearance:0.33||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:DBP6(YNR038W)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.33||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:GPI11(YDR302W)|FD-Score:-3.41|P-value:3.24E-4|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:NUF2(YOL069W)|FD-Score:3.73|P-value:9.67E-5|Clearance:0.33||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUT2(YPR168W)|FD-Score:3.14|P-value:8.55E-4|Clearance:0.07||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:ORC4(YPR162C)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.33||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RFA1(YAR007C)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RPC25(YKL144C)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPS13(YDR064W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SNP1(YIL061C)|FD-Score:3.25|P-value:5.79E-4|Clearance:0.11||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:UBA1(YKL210W)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.12||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:ULP1(YPL020C)|FD-Score:-3.44|P-value:2.93E-4|Clearance:0||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:YGR114C(YGR114C_d)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YPL238C(YPL238C_d)|FD-Score:3.28|P-value:5.17E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR177C(YPR177C_d)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9634651
Download HOP data (tab-delimited text)  (excel)
Gene:CAF130(YGR134W)|FD-Score:4.76|P-value:9.69E-7||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CAF4(YKR036C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CEX1(YOR112W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COG5(YNL051W)|FD-Score:-5.83|P-value:2.74E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-4.65|P-value:1.64E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-4.27|P-value:9.63E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EAP1(YKL204W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EOS1(YNL080C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ESC2(YDR363W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FIG4(YNL325C)|FD-Score:7.77|P-value:3.93E-15||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GDH1(YOR375C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GPB1(YOR371C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HAP2(YGL237C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HCS1(YKL017C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HRD1(YOL013C)|FD-Score:4.41|P-value:5.25E-6||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IRS4(YKR019C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IZH3(YLR023C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:KEL3(YPL263C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:KTI11(YBL071W-A)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LOA1(YPR139C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MCH2(YKL221W)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MNR2(YKL064W)|FD-Score:5.34|P-value:4.62E-8||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MOS1(YCL057C-A)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MSS1(YMR023C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NNF2(YGR089W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PCL10(YGL134W)|FD-Score:3.29|P-value:5.10E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PEX12(YMR026C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PFS1(YHR185C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:POF1(YCL047C)|FD-Score:7.68|P-value:7.96E-15||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:POR1(YNL055C)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PPN1(YDR452W)|FD-Score:-4.5|P-value:3.40E-6||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PRR1(YKL116C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PSO2(YMR137C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD16(YBR114W)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD24(YER173W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RAX2(YLR084C)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RGP1(YDR137W)|FD-Score:-5.38|P-value:3.68E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS24A(YER074W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SER3(YER081W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLP1(YOR154W_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SPT3(YDR392W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRL2(YLR082C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SRN2(YLR119W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:STB5(YHR178W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:TEF4(YKL081W)|FD-Score:-5.03|P-value:2.45E-7||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TLG2(YOL018C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRM13(YOL125W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UBP15(YMR304W)|FD-Score:-3.79|P-value:7.51E-5||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:URA8(YJR103W)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-6.86|P-value:3.53E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.46|P-value:2.36E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS9(YML097C)|FD-Score:4.97|P-value:3.36E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YAL004W(YAL004W_d)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL066W(YAL066W_d)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR138C(YBR138C_p)|FD-Score:5.31|P-value:5.57E-8||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:6.27|P-value:1.78E-10||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDR391C(YDR391C_p)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YET2(YMR040W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YFH7(YFR007W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YFL052W(YFL052W_p)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHL012W(YHL012W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YIL108W(YIL108W_p)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL118W(YJL118W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YLR402W(YLR402W_d)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL035C(YNL035C_p)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL324W(YNL324W_d)|FD-Score:13.7|P-value:2.72E-43||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR186W(YOR186W_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPK9(YOR291W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPL041C(YPL041C_p)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPT6(YLR262C)|FD-Score:-5.98|P-value:1.14E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:CAF130(YGR134W)|FD-Score:4.76|P-value:9.69E-7||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CAF4(YKR036C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CEX1(YOR112W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COG5(YNL051W)|FD-Score:-5.83|P-value:2.74E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-4.65|P-value:1.64E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-4.27|P-value:9.63E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:EAP1(YKL204W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EOS1(YNL080C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ESC2(YDR363W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FIG4(YNL325C)|FD-Score:7.77|P-value:3.93E-15||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GDH1(YOR375C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GPB1(YOR371C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HAP2(YGL237C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HCS1(YKL017C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HRD1(YOL013C)|FD-Score:4.41|P-value:5.25E-6||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IRS4(YKR019C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IZH3(YLR023C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:KEL3(YPL263C)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:KTI11(YBL071W-A)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LOA1(YPR139C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MCH2(YKL221W)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MNR2(YKL064W)|FD-Score:5.34|P-value:4.62E-8||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MOS1(YCL057C-A)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MSS1(YMR023C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NNF2(YGR089W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PCL10(YGL134W)|FD-Score:3.29|P-value:5.10E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PEX12(YMR026C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PFS1(YHR185C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:POF1(YCL047C)|FD-Score:7.68|P-value:7.96E-15||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:POR1(YNL055C)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PPN1(YDR452W)|FD-Score:-4.5|P-value:3.40E-6||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PRR1(YKL116C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PSO2(YMR137C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD16(YBR114W)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD24(YER173W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RAX2(YLR084C)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RGP1(YDR137W)|FD-Score:-5.38|P-value:3.68E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.56|P-value:2.55E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS24A(YER074W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:-4.64|P-value:1.74E-6||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SER3(YER081W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLP1(YOR154W_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p Gene:SPT3(YDR392W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRL2(YLR082C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SRN2(YLR119W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:STB5(YHR178W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:TEF4(YKL081W)|FD-Score:-5.03|P-value:2.45E-7||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TLG2(YOL018C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRM13(YOL125W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UBP15(YMR304W)|FD-Score:-3.79|P-value:7.51E-5||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:URA8(YJR103W)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-6.86|P-value:3.53E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.46|P-value:2.36E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS9(YML097C)|FD-Score:4.97|P-value:3.36E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YAL004W(YAL004W_d)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL066W(YAL066W_d)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR138C(YBR138C_p)|FD-Score:5.31|P-value:5.57E-8||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:6.27|P-value:1.78E-10||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDR391C(YDR391C_p)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YET2(YMR040W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YFH7(YFR007W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YFL052W(YFL052W_p)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHL012W(YHL012W_p)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YIL108W(YIL108W_p)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL118W(YJL118W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YLR402W(YLR402W_d)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL035C(YNL035C_p)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL324W(YNL324W_d)|FD-Score:13.7|P-value:2.72E-43||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR186W(YOR186W_p)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPK9(YOR291W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPL041C(YPL041C_p)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPT6(YLR262C)|FD-Score:-5.98|P-value:1.14E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPR162C4.211.28E-50.33ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YGR114C_d3.993.36E-50.33YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YNR038W3.914.62E-50.33DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YPR176C3.846.12E-50.33BET2Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YOL069W3.739.67E-50.33NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YKL210W3.403.41E-40.12UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YPL238C_d3.285.17E-40.01YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YDR064W3.275.37E-40.02RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YIL061C3.255.79E-40.11SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YPR168W3.148.55E-40.07NUT2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
YMR281W3.070.001080.22GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YMR047C2.840.002240.01NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
YOL134C_d2.830.002320.12YOL134C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
YNL182C2.710.003400.03IPI3Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene
YFL024C2.680.003730.06EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL324W_d13.702.72E-43YNL324W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL325C7.773.93E-15FIG4Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain
YCL047C7.687.96E-15POF1ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p
YCL002C_p6.271.78E-10YCL002C_pPutative protein of unknown function; YCL002C is not an essential gene
YKL064W5.344.62E-8MNR2Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations
YBR138C_p5.315.57E-8YBR138C_pCytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
YCL057C-A5.111.63E-7MOS1Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1
YML097C4.973.36E-7VPS9A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
YGR134W4.769.69E-7CAF130Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation
YNL055C4.434.62E-6POR1Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication
YOL013C4.415.25E-6HRD1Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
YJR103W4.385.84E-6URA8Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
YOR375C4.241.13E-5GDH1NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
YOR291W4.231.19E-5YPK9Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
YKL116C4.072.31E-5PRR1Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor

GO enrichment analysis for SGTC_865
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1802.73E-44SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.0701754ergosterol biosynthesis
0.1783.13E-43SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.102941
0.1511.20E-31SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride 61.8 μMTimTec (Natural product derivative library)170377580.0735294ergosterol biosynthesis
0.1424.90E-28SGTC_830170-0301 39.0 μMChemDiv (Drug-like library)30897160.0181818endomembrane recycling
0.1411.92E-27SGTC_21075363541 170.2 μMChembridge (Fragment library)2440520.0701754
0.1375.99E-26SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.1fatty acid desaturase (OLE1)
0.1367.24E-26SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.0746269
0.1331.36E-24SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.104478fatty acid desaturase (OLE1)
0.1255.31E-22SGTC_21856113692 200.0 μMChembridge (Fragment library)8741500.05ergosterol biosynthesis
0.1233.29E-21SGTC_7064262-0057 46.9 μMChemDiv (Drug-like library)8832980.0535714fatty acid desaturase (OLE1)
0.1194.64E-20SGTC_8710574-0004 149.0 μMChemDiv (Drug-like library)95511730.145161
0.1194.78E-20SGTC_30549094397 49.5 μMChembridge (Drug-like library)25983540.0983607fatty acid desaturase (OLE1)
0.1165.10E-19SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.056338plasma membrane duress
0.1094.15E-17SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.0566038plasma membrane duress
0.1062.32E-16SGTC_1752st044819 83.0 μMTimTec (Natural product derivative library)4412090.111111

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2427554505542.75 μM0.3829795372858Miscellaneous250.298463.71113
SGTC_1165k072-027530.3 μM0.3333336153523ChemDiv (Drug-like library)211.262422.78413
SGTC_774k060-0086136 μM0.3207555911235ChemDiv (Drug-like library)280.324443.67815
SGTC_623k072-010816.9 μM0.325382803ChemDiv (Drug-like library)254.31032.92315heme biosynthesis & mitochondrial translocase
SGTC_24435493264126.67 μM0.3137265340055Miscellaneous269.321642.39725
SGTC_208k072-009812.37 μM0.31255948901ChemDiv (Drug-like library)231.68092.96213heme biosynthesis & mitochondrial translocase
SGTC_7334069-000756.1 μM0.3018875717425ChemDiv (Drug-like library)309.193764.32814ERAD & cell cycle
SGTC_2424553792859.2 μM0.3018875382802Miscellaneous254.31032.92315
SGTC_1883229-0966278.47 μM0.2962965722370ChemDiv (Drug-like library)269.321643.83225
SGTC_243954920307.2 μM0.2962965722370Miscellaneous269.321643.83225