0527-0199

3-[2-(4-bromophenyl)iminohydrazinyl]-5-nitrobenzoic acid

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_868
Screen concentration 213.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2849450
SMILES C1=CC(=CC=C1N=NNC2=CC(=CC(=C2)C(=O)O)[N+](=O)[O-])Br
Standardized SMILES OC(=O)c1cc(NN=Nc2ccc(Br)cc2)cc(c1)[N+](=O)[O-]
Molecular weight 365.139
ALogP 3.86
H-bond donor count 2
H-bond acceptor count 7
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 33.2
% growth inhibition (Hom. pool) 7.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2849450
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:-3.43|P-value:3.00E-4|Clearance:0||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:BET3(YKR068C)|FD-Score:3.17|P-value:7.72E-4|Clearance:0.08||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CDC9(YDL164C)|FD-Score:6.14|P-value:4.10E-10|Clearance:0.27||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:ERG11(YHR007C)|FD-Score:12.7|P-value:3.15E-37|Clearance:4.85||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.1||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:5.87|P-value:2.22E-9|Clearance:0.43||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:FOL3(YMR113W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GPI13(YLL031C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:RPS15(YOL040C)|FD-Score:3.79|P-value:7.48E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP4(YHR069C)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.27||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SUI2(YJR007W)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.43||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TID3(YIL144W)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.04||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YJL086C(YJL086C_d)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.54||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:5.43|P-value:2.76E-8|Clearance:0.8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL238C(YPL238C_d)|FD-Score:7.84|P-value:2.18E-15|Clearance:1.7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:AAR2(YBL074C)|FD-Score:-3.43|P-value:3.00E-4|Clearance:0||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:BET3(YKR068C)|FD-Score:3.17|P-value:7.72E-4|Clearance:0.08||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CDC9(YDL164C)|FD-Score:6.14|P-value:4.10E-10|Clearance:0.27||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:ERG11(YHR007C)|FD-Score:12.7|P-value:3.15E-37|Clearance:4.85||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:4.21|P-value:1.29E-5|Clearance:0.1||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:ERG29(YMR134W_p)|FD-Score:5.87|P-value:2.22E-9|Clearance:0.43||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:FOL3(YMR113W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GPI13(YLL031C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:RPS15(YOL040C)|FD-Score:3.79|P-value:7.48E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP4(YHR069C)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.27||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SUI2(YJR007W)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.43||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TID3(YIL144W)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.04||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YJL086C(YJL086C_d)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.54||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:5.43|P-value:2.76E-8|Clearance:0.8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL238C(YPL238C_d)|FD-Score:7.84|P-value:2.18E-15|Clearance:1.7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2849450
Download HOP data (tab-delimited text)  (excel)
Gene:ACN9(YDR511W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AFT1(YGL071W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO2(YGL148W)|FD-Score:3.82|P-value:6.59E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG36(YJL185C)|FD-Score:-4.56|P-value:2.59E-6||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:ATP14(YLR295C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:4.9|P-value:4.71E-7||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BUD7(YOR299W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:5.22|P-value:9.03E-8||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CEX1(YOR112W)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CHS7(YHR142W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLN2(YPL256C)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CNE1(YAL058W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:COG5(YNL051W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CTA1(YDR256C)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CYS3(YAL012W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:-4.3|P-value:8.63E-6||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DRS2(YAL026C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ERG2(YMR202W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FET4(YMR319C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FPR4(YLR449W)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GAL80(YML051W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GAS1(YMR307W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN5(YGR252W)|FD-Score:6.02|P-value:8.76E-10||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:27.7|P-value:1.38E-169||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GTO1(YGR154C)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:GYP1(YOR070C)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HHO1(YPL127C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:ILV1(YER086W)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ISA2(YPR067W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JJJ2(YJL162C)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KTI11(YBL071W-A)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MDJ2(YNL328C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MEP3(YPR138C)|FD-Score:-4.36|P-value:6.61E-6||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MRPL22(YNL177C)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS11(YMR164C)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:OCA6(YDR067C_p)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PAC10(YGR078C)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PCL1(YNL289W)|FD-Score:-4.3|P-value:8.68E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX14(YGL153W)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PHO13(YDL236W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PHO87(YCR037C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PHO88(YBR106W)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIN2(YOR104W)|FD-Score:6.97|P-value:1.53E-12||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PPT2(YPL148C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PYC2(YBR218C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:10.6|P-value:9.13E-27||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RER1(YCL001W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RIM21(YNL294C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL19A(YBR084C-A)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIL1(YOL031C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SPS100(YHR139C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRX1(YKL086W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SWA2(YDR320C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:THI12(YNL332W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:TRS85(YDR108W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBX4(YMR067C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VAC7(YNL054W)|FD-Score:5.43|P-value:2.86E-8||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VMA7(YGR020C)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS1(YKR001C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:WSC2(YNL283C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL032W(YDL032W_d)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR401W(YDR401W_d)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YHR180W(YHR180W_d)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLL054C(YLL054C_p)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR311C(YLR311C_d)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR027W(YMR027W_p)|FD-Score:-3.84|P-value:6.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL043C(YNL043C_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL050C(YNL050C_p)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function Gene:YPL261C(YPL261C_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR022C(YPR022C_p)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPT31(YER031C)|FD-Score:9.77|P-value:7.95E-23||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ACN9(YDR511W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AFT1(YGL071W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO2(YGL148W)|FD-Score:3.82|P-value:6.59E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG36(YJL185C)|FD-Score:-4.56|P-value:2.59E-6||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:ATP14(YLR295C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:4.9|P-value:4.71E-7||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:5.17|P-value:1.14E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BUD7(YOR299W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:5.22|P-value:9.03E-8||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CEX1(YOR112W)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CHS7(YHR142W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLN2(YPL256C)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CNE1(YAL058W)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:COG5(YNL051W)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CTA1(YDR256C)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CYS3(YAL012W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:-4.3|P-value:8.63E-6||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DRS2(YAL026C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ERG2(YMR202W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FET4(YMR319C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FPR4(YLR449W)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GAL80(YML051W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GAS1(YMR307W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN5(YGR252W)|FD-Score:6.02|P-value:8.76E-10||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:27.7|P-value:1.38E-169||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GTO1(YGR154C)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:GYP1(YOR070C)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HHO1(YPL127C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:ILV1(YER086W)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ISA2(YPR067W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JJJ2(YJL162C)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KTI11(YBL071W-A)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MDJ2(YNL328C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MEP3(YPR138C)|FD-Score:-4.36|P-value:6.61E-6||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MRPL22(YNL177C)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS11(YMR164C)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:OCA6(YDR067C_p)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PAC10(YGR078C)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PCL1(YNL289W)|FD-Score:-4.3|P-value:8.68E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX14(YGL153W)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PHO13(YDL236W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PHO87(YCR037C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PHO88(YBR106W)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIN2(YOR104W)|FD-Score:6.97|P-value:1.53E-12||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PPT2(YPL148C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PYC2(YBR218C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:10.6|P-value:9.13E-27||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RER1(YCL001W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RIM21(YNL294C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL19A(YBR084C-A)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIL1(YOL031C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SPS100(YHR139C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRX1(YKL086W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SWA2(YDR320C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:THI12(YNL332W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:TRS85(YDR108W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBX4(YMR067C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VAC7(YNL054W)|FD-Score:5.43|P-value:2.86E-8||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VMA7(YGR020C)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS1(YKR001C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:WSC2(YNL283C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDL032W(YDL032W_d)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR401W(YDR401W_d)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YHR180W(YHR180W_d)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLL054C(YLL054C_p)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR311C(YLR311C_d)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR027W(YMR027W_p)|FD-Score:-3.84|P-value:6.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL043C(YNL043C_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL050C(YNL050C_p)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Putative protein of unknown function Gene:YPL261C(YPL261C_d)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR022C(YPR022C_p)|FD-Score:5.1|P-value:1.69E-7||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPT31(YER031C)|FD-Score:9.77|P-value:7.95E-23||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR007C12.703.15E-374.85ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YPL238C_d7.842.18E-151.70YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YDL164C6.144.10E-100.27CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YMR134W_p5.872.22E-90.43ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YJL195C_d5.432.76E-80.80YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YJR007W4.631.81E-60.42SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YGR060W4.211.29E-50.10ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YIL144W4.112.02E-50.04TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YHR069C4.072.39E-50.27RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YOL040C3.797.48E-50.08RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YJL086C_d3.711.04E-40.54YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YKR068C3.177.72E-40.08BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YOR319W3.080.001030.10HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YOR224C2.990.001410.02RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YPR187W2.960.001520.02RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL226C27.701.38E-169GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YJL204C10.609.13E-27RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YER031C9.777.95E-23YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
YBR106W7.331.16E-13PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YOR104W6.971.53E-12PIN2Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated
YGR252W6.028.76E-10GCN5Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
YNL010W_p5.601.10E-8YNL010W_pPutative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation
YPL256C5.551.39E-8CLN2G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
YNL054W5.432.86E-8VAC7Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock
YCR094W5.229.03E-8CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YAL058W5.181.12E-7CNE1Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
YPL069C5.171.14E-7BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDR256C5.171.18E-7CTA1Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation
YPR022C_p5.101.69E-7YPR022C_pPutative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YGR020C5.091.79E-7VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane

GO enrichment analysis for SGTC_868
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5090SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.278689fatty acid desaturase (OLE1)
0.3953.17E-219SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0483871fatty acid desaturase (OLE1)
0.3885.19E-211SGTC_258haloperidol 50.8 μMMiscellaneous35590.102564fatty acid desaturase (OLE1)
0.3814.96E-203SGTC_7680384-0033 77.3 μMChemDiv (Drug-like library)39653050.193548fatty acid desaturase (OLE1)
0.3544.69E-174SGTC_13081187-1617 41.4 μMChemDiv (Drug-like library)28825700.0666667fatty acid desaturase (OLE1)
0.3533.54E-173SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.0506329fatty acid desaturase (OLE1)
0.3427.12E-162SGTC_254nsc-64875 3.8 μMMiscellaneous56993NAergosterol depletion effects on membrane
0.3411.21E-160SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.0526316fatty acid desaturase (OLE1)
0.3219.70E-142SGTC_577r071-0003 38.6 μMChemDiv (Drug-like library)45685170.0634921fatty acid desaturase (OLE1)
0.3189.63E-139SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.118421fatty acid desaturase (OLE1)
0.3051.39E-127SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.112676fatty acid desaturase (OLE1)
0.3043.19E-126SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.103896fatty acid desaturase (OLE1)
0.3032.54E-125SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.0519481plasma membrane duress
0.3021.08E-124SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.0597015fatty acid desaturase (OLE1)
0.2979.78E-121SGTC_8340312-0008 75.7 μMChemDiv (Drug-like library)63855730.214286NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6880527-0193473 μM0.644007154ChemDiv (Drug-like library)286.24293.10827fatty acid desaturase (OLE1)
SGTC_990109-0120622.8 μM0.431373759414ChemDiv (Drug-like library)321.126163.25713
SGTC_1150527-0192112.04 μM0.3928574233931ChemDiv (Drug-like library)276.678464.14315
SGTC_1340269-00186.92 μM0.3928574378915ChemDiv (Drug-like library)411.121283.04617
SGTC_12590671-013243 μM0.338983X1259ChemDiv (Drug-like library)371.206443.44641
SGTC_5671080-054268.4 μM0.3333335950415ChemDiv (Drug-like library)296.277483.3115
SGTC_9262268-140159.4 μM0.3281252931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_6371181-05197.26 μM0.3275866252091ChemDiv (Drug-like library)250.253860.70925fatty acid desaturase (OLE1)
SGTC_750159-0059199.01 μM0.3166675377270ChemDiv (Drug-like library)251.238621.99916fatty acid desaturase (OLE1)
SGTC_7251497-096319 μM0.3050853549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress