0574-0004

phenyl N-[(Z)-2-cyano-3-(4-methylphenyl)prop-2-enoyl]carbamate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_871
Screen concentration 149.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 9551173
SMILES CC1=CC=C(C=C1)C=C(C#N)C(=O)NC(=O)OC2=CC=CC=C2
Standardized SMILES Cc1ccc(C=C(C#N)C(=O)NC(=O)Oc2ccccc2)cc1
Molecular weight 306.3154
ALogP 3.84
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.38
% growth inhibition (Hom. pool) 10.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9551173
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.15||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ACC1(YNR016C)|FD-Score:9.39|P-value:3.02E-21|Clearance:0.27||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:10.2|P-value:1.32E-24|Clearance:0.5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:6.33|P-value:1.24E-10|Clearance:0.34||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:5.34|P-value:4.56E-8|Clearance:0.29||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DAM1(YGR113W)|FD-Score:3.86|P-value:5.76E-5|Clearance:0.02||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:DFR1(YOR236W)|FD-Score:9.12|P-value:3.82E-20|Clearance:0.07||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FCP1(YMR277W)|FD-Score:4.32|P-value:7.71E-6|Clearance:0.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LSM2(YBL026W)|FD-Score:4.29|P-value:8.80E-6|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NSE4(YDL105W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP145(YGL092W)|FD-Score:4.43|P-value:4.61E-6|Clearance:0.11||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:NUP49(YGL172W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP57(YGR119C)|FD-Score:3.83|P-value:6.31E-5|Clearance:0.04||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PAB1(YER165W)|FD-Score:11.4|P-value:1.34E-30|Clearance:1.07||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:RAD53(YPL153C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.01||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO1(YPR165W)|FD-Score:5.99|P-value:1.07E-9|Clearance:0.64||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB3(YIL021W)|FD-Score:6.34|P-value:1.15E-10|Clearance:0.01||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB5(YBR154C)|FD-Score:4.87|P-value:5.59E-7|Clearance:0.19||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB7(YDR404C)|FD-Score:9.05|P-value:7.16E-20|Clearance:0.08||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL32(YBL092W)|FD-Score:5.05|P-value:2.16E-7|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPO26(YPR187W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPO31(YOR116C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.06||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RSC58(YLR033W)|FD-Score:13.4|P-value:3.64E-41|Clearance:1.95||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:8.97|P-value:1.47E-19|Clearance:0.88||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:10.4|P-value:1.73E-25|Clearance:0.2||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RVB1(YDR190C)|FD-Score:4.44|P-value:4.53E-6|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SFH1(YLR321C)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.32||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SNU23(YDL098C)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.31||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPB4(YFL002C)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.03||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC105(YGL093W)|FD-Score:8.09|P-value:3.08E-16|Clearance:0.24||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STH1(YIL126W)|FD-Score:9.67|P-value:2.05E-22|Clearance:0.28||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUP35(YDR172W)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.24||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFC3(YAL001C)|FD-Score:6.36|P-value:1.01E-10|Clearance:0.02||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIM12(YBR091C)|FD-Score:7.58|P-value:1.70E-14|Clearance:1.22||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:YEF3(YLR249W)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.09||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YJL195C(YJL195C_d)|FD-Score:-5.07|P-value:2.01E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTH1(YPR107C)|FD-Score:7.84|P-value:2.22E-15|Clearance:0.26||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:AAR2(YBL074C)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.15||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ACC1(YNR016C)|FD-Score:9.39|P-value:3.02E-21|Clearance:0.27||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:10.2|P-value:1.32E-24|Clearance:0.5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:6.33|P-value:1.24E-10|Clearance:0.34||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:5.34|P-value:4.56E-8|Clearance:0.29||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DAM1(YGR113W)|FD-Score:3.86|P-value:5.76E-5|Clearance:0.02||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:DFR1(YOR236W)|FD-Score:9.12|P-value:3.82E-20|Clearance:0.07||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:FCP1(YMR277W)|FD-Score:4.32|P-value:7.71E-6|Clearance:0.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LSM2(YBL026W)|FD-Score:4.29|P-value:8.80E-6|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NSE4(YDL105W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:NUP145(YGL092W)|FD-Score:4.43|P-value:4.61E-6|Clearance:0.11||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:NUP49(YGL172W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP57(YGR119C)|FD-Score:3.83|P-value:6.31E-5|Clearance:0.04||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PAB1(YER165W)|FD-Score:11.4|P-value:1.34E-30|Clearance:1.07||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:RAD53(YPL153C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.01||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO1(YPR165W)|FD-Score:5.99|P-value:1.07E-9|Clearance:0.64||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB3(YIL021W)|FD-Score:6.34|P-value:1.15E-10|Clearance:0.01||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB5(YBR154C)|FD-Score:4.87|P-value:5.59E-7|Clearance:0.19||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB7(YDR404C)|FD-Score:9.05|P-value:7.16E-20|Clearance:0.08||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL32(YBL092W)|FD-Score:5.05|P-value:2.16E-7|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPO26(YPR187W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPO31(YOR116C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.06||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RSC58(YLR033W)|FD-Score:13.4|P-value:3.64E-41|Clearance:1.95||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:8.97|P-value:1.47E-19|Clearance:0.88||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:10.4|P-value:1.73E-25|Clearance:0.2||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RVB1(YDR190C)|FD-Score:4.44|P-value:4.53E-6|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SFH1(YLR321C)|FD-Score:3.8|P-value:7.32E-5|Clearance:0.32||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SNU23(YDL098C)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.31||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPB4(YFL002C)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.03||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC105(YGL093W)|FD-Score:8.09|P-value:3.08E-16|Clearance:0.24||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STH1(YIL126W)|FD-Score:9.67|P-value:2.05E-22|Clearance:0.28||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUP35(YDR172W)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.24||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFC3(YAL001C)|FD-Score:6.36|P-value:1.01E-10|Clearance:0.02||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIM12(YBR091C)|FD-Score:7.58|P-value:1.70E-14|Clearance:1.22||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:YEF3(YLR249W)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.09||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YJL195C(YJL195C_d)|FD-Score:-5.07|P-value:2.01E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTH1(YPR107C)|FD-Score:7.84|P-value:2.22E-15|Clearance:0.26||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9551173
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACN9(YDR511W)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AFT1(YGL071W)|FD-Score:-6.34|P-value:1.12E-10||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO1(YDR127W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP6(YLR085C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATP14(YLR295C)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AXL1(YPR122W)|FD-Score:7.03|P-value:1.01E-12||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BTS1(YPL069C)|FD-Score:9|P-value:1.09E-19||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUL1(YMR275C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CIN1(YOR349W)|FD-Score:6.06|P-value:6.61E-10||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:12|P-value:1.33E-33||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB2(YPR119W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CNN1(YFR046C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COG5(YNL051W)|FD-Score:-5.65|P-value:7.85E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.95|P-value:3.93E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:13.3|P-value:9.73E-41||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DGR2(YKL121W_p)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIA4(YHR011W)|FD-Score:5.3|P-value:5.67E-8||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIF1(YLR437C)|FD-Score:5.9|P-value:1.78E-9||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:EAF1(YDR359C)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ENV10(YLR065C)|FD-Score:6.07|P-value:6.24E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ESBP6(YNL125C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:EXG1(YLR300W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FBP26(YJL155C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FIG1(YBR040W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:FRE6(YLL051C)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:FYV10(YIL097W)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAL1(YBR020W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:4.41|P-value:5.29E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GYP1(YOR070C)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HMG1(YML075C)|FD-Score:8.81|P-value:6.27E-19||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:5.21|P-value:9.52E-8||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IES2(YNL215W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:-6.3|P-value:1.46E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JJJ3(YJR097W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LNP1(YHR192W_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LSM7(YNL147W)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LST7(YGR057C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LTE1(YAL024C)|FD-Score:15.7|P-value:7.66E-56||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCM21(YDR318W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MED1(YPR070W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET22(YOL064C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MOD5(YOR274W)|FD-Score:-4.41|P-value:5.19E-6||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL38(YKL170W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MTM1(YGR257C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MUD1(YBR119W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MZM1(YDR493W)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NNF2(YGR089W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NOP13(YNL175C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:OYE2(YHR179W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET111(YMR257C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:POL32(YJR043C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PSP2(YML017W)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTK2(YJR059W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RGP1(YDR137W)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.89|P-value:5.02E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RNR3(YIL066C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPH1(YER169W)|FD-Score:6.33|P-value:1.25E-10||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:RXT2(YBR095C)|FD-Score:4.52|P-value:3.02E-6||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SCS2(YER120W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SET2(YJL168C)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SIS2(YKR072C)|FD-Score:5.23|P-value:8.63E-8||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SOH1(YGL127C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRL2(YLR082C)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SRO9(YCL037C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRX1(YKL086W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SUR2(YDR297W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWC3(YAL011W)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWC5(YBR231C)|FD-Score:7.06|P-value:8.45E-13||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TDA7(YNL176C_p)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TEF4(YKL081W)|FD-Score:-4.86|P-value:5.93E-7||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TFB5(YDR079C-A)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TFS1(YLR178C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP3(YKL211C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-4.34|P-value:7.04E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:19.4|P-value:1.53E-84||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UPS1(YLR193C)|FD-Score:5.24|P-value:8.14E-8||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VAC14(YLR386W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VFA1(YER128W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS52(YDR484W)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:-5.93|P-value:1.49E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:7.62|P-value:1.29E-14||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBL059W(YBL059W_p)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL062W(YBL062W_d)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W(YBR056W_p)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDR431W(YDR431W_d)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR537C(YDR537C_d)|FD-Score:6.14|P-value:4.11E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEL043W(YEL043W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER188W(YER188W_d)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YFH7(YFR007W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL072C(YGL072C_d)|FD-Score:4.28|P-value:9.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL047C-A(YJL047C-A_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function Gene:YJL120W(YJL120W_d)|FD-Score:4.76|P-value:9.56E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL182C(YJL182C_d)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJL193W(YJL193W_p)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YJR003C(YJR003C_p)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL075C(YKL075C_p)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKR047W(YKR047W_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR415C(YLR415C_p)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function Gene:YNL010W(YNL010W_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL324W(YNL324W_d)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:12.4|P-value:1.19E-35||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-4.27|P-value:9.64E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ACB1(YGR037C)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACN9(YDR511W)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AFT1(YGL071W)|FD-Score:-6.34|P-value:1.12E-10||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO1(YDR127W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP6(YLR085C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATP14(YLR295C)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AXL1(YPR122W)|FD-Score:7.03|P-value:1.01E-12||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BTS1(YPL069C)|FD-Score:9|P-value:1.09E-19||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUL1(YMR275C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CIN1(YOR349W)|FD-Score:6.06|P-value:6.61E-10||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:12|P-value:1.33E-33||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB2(YPR119W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CNN1(YFR046C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COG5(YNL051W)|FD-Score:-5.65|P-value:7.85E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.95|P-value:3.93E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:-4.03|P-value:2.80E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:13.3|P-value:9.73E-41||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DGR2(YKL121W_p)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIA4(YHR011W)|FD-Score:5.3|P-value:5.67E-8||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIF1(YLR437C)|FD-Score:5.9|P-value:1.78E-9||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:EAF1(YDR359C)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ENV10(YLR065C)|FD-Score:6.07|P-value:6.24E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ESBP6(YNL125C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:EXG1(YLR300W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FBP26(YJL155C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FIG1(YBR040W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:FRE6(YLL051C)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:FYV10(YIL097W)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAL1(YBR020W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:4.41|P-value:5.29E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GYP1(YOR070C)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HMG1(YML075C)|FD-Score:8.81|P-value:6.27E-19||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:5.21|P-value:9.52E-8||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IES2(YNL215W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:-6.3|P-value:1.46E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JJJ3(YJR097W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LNP1(YHR192W_p)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LSM7(YNL147W)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:LST7(YGR057C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LTE1(YAL024C)|FD-Score:15.7|P-value:7.66E-56||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCM21(YDR318W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MED1(YPR070W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET22(YOL064C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MOD5(YOR274W)|FD-Score:-4.41|P-value:5.19E-6||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL38(YKL170W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MTM1(YGR257C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MUD1(YBR119W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MZM1(YDR493W)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NNF2(YGR089W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NOP13(YNL175C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:OYE2(YHR179W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET111(YMR257C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:POL32(YJR043C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PSP2(YML017W)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTK2(YJR059W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RGP1(YDR137W)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.89|P-value:5.02E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RNR3(YIL066C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPH1(YER169W)|FD-Score:6.33|P-value:1.25E-10||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:RXT2(YBR095C)|FD-Score:4.52|P-value:3.02E-6||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SCS2(YER120W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SET2(YJL168C)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SIS2(YKR072C)|FD-Score:5.23|P-value:8.63E-8||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SOH1(YGL127C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRL2(YLR082C)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:SRO9(YCL037C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRX1(YKL086W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SUR2(YDR297W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWC3(YAL011W)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWC5(YBR231C)|FD-Score:7.06|P-value:8.45E-13||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TDA7(YNL176C_p)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TEF4(YKL081W)|FD-Score:-4.86|P-value:5.93E-7||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TFB5(YDR079C-A)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TFS1(YLR178C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP3(YKL211C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-4.34|P-value:7.04E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:19.4|P-value:1.53E-84||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UPS1(YLR193C)|FD-Score:5.24|P-value:8.14E-8||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VAC14(YLR386W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VFA1(YER128W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS52(YDR484W)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS61(YDR136C_d)|FD-Score:-5.93|P-value:1.49E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:7.62|P-value:1.29E-14||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBL059W(YBL059W_p)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL062W(YBL062W_d)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W(YBR056W_p)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YDR431W(YDR431W_d)|FD-Score:-3.42|P-value:3.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR537C(YDR537C_d)|FD-Score:6.14|P-value:4.11E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEL043W(YEL043W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER188W(YER188W_d)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YFH7(YFR007W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL072C(YGL072C_d)|FD-Score:4.28|P-value:9.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL047C-A(YJL047C-A_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function Gene:YJL120W(YJL120W_d)|FD-Score:4.76|P-value:9.56E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL182C(YJL182C_d)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YJL193W(YJL193W_p)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YJR003C(YJR003C_p)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL075C(YKL075C_p)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKR047W(YKR047W_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR415C(YLR415C_p)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Putative protein of unknown function Gene:YNL010W(YNL010W_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL324W(YNL324W_d)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:12.4|P-value:1.19E-35||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-4.27|P-value:9.64E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W13.403.64E-411.95RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YER165W11.401.34E-301.07PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YML127W10.401.73E-250.20RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YJL081C10.201.32E-240.50ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YIL126W9.672.05E-220.28STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YNR016C9.393.02E-210.27ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YOR236W9.123.82E-200.07DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YDR404C9.057.16E-200.08RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YFR037C8.971.47E-190.89RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YGL093W8.093.08E-160.24SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YPR107C7.842.22E-150.26YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YBR091C7.581.70E-141.22TIM12Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
YAL001C6.361.01E-100.02TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YIL021W6.341.15E-100.01RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YPR034W6.331.24E-100.34ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A19.401.53E-84TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YAL024C15.707.66E-56LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YGR092W13.309.73E-41DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YKL126W12.401.19E-35YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YNL298W12.001.33E-33CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YPL069C9.001.09E-19BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YML075C8.816.27E-19HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
YDR485C7.621.29E-14VPS72Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YBR231C7.068.45E-13SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YPR122W7.031.01E-12AXL1Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
YER169W6.331.25E-10RPH1JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
YDR537C_d6.144.11E-10YDR537C_dDubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
YLR065C6.076.24E-10ENV10Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
YOR349W6.066.61E-10CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YLR437C5.901.78E-9DIF1Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein

GO enrichment analysis for SGTC_871
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5060SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.109375
0.4073.24E-234SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.306452RSC complex & mRNA processing
0.3968.32E-221SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0675676RSC complex & mRNA processing
0.3855.31E-208SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.157895RSC complex & mRNA processing
0.3795.63E-201SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.126761RSC complex & mRNA processing
0.3715.29E-192SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.131148TSC3-RPN4
0.3575.63E-177SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.119403RSC complex & mRNA processing
0.3533.63E-173SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0533333RSC complex & mRNA processing
0.3512.56E-170SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.107143
0.3414.64E-160SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.2
0.3409.09E-160SGTC_5941487-1584 10.8 μMChemDiv (Drug-like library)50133320.0769231
0.3366.48E-156SGTC_1651st011928 86.1 μMTimTec (Natural product derivative library)7201980.25RSC complex & mRNA processing
0.3271.50E-147SGTC_14934348-0038 111.0 μMChemDiv (Drug-like library)8004240.0857143RSC complex & mRNA processing
0.3218.92E-141SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.141026
0.3072.75E-128SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0923077RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_13621611-0397101 μM0.44680927816ChemDiv (Drug-like library)227.258523.65612
SGTC_510ag-490170 μM0.3965525328779ICCB bioactive library294.304662.44434
SGTC_1652st01193278 μM0.3064521551076TimTec (Natural product derivative library)245.230742.10905RSC complex & mRNA processing
SGTC_7521896-0090130 μM0.305085772567ChemDiv (Drug-like library)294.147182.04913TSC3-RPN4
SGTC_3260913710949.47 μM0.30357117478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_12890976-007369.1 μM0.2777783391136ChemDiv (Drug-like library)253.29582.7812
SGTC_503tyrphostin 8294 μM0.26530692970ICCB bioactive library170.167441.77313TSC3-RPN4
SGTC_2984902210938.96 μM0.2580656459207Chembridge (Drug-like library)269.338263.57512
SGTC_1985st07138265.5 μM0.2539686123237TimTec (Natural product derivative library)305.325762.66906
SGTC_3091911637249.47 μM0.25396817169793Chembridge (Drug-like library)285.337662.95313