0771-0151

2-methyl-1-[(E)-2-(4-nitrophenyl)ethenyl]sulfonylimidazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_877
Screen concentration 33.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 6166523
SMILES CC1=NC=CN1S(=O)(=O)C=CC2=CC=C(C=C2)[N+](=O)[O-]
Standardized SMILES Cc1nccn1S(=O)(=O)C=Cc2ccc(cc2)[N+](=O)[O-]
Molecular weight 293.2984
ALogP 1.17
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.58
% growth inhibition (Hom. pool) 2.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6166523
Download HIP data (tab-delimited text)  (excel)
Gene:BDP1(YNL039W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.03||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DML1(YMR211W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.11||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG13(YML126C)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.06||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:GUS1(YGL245W)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.07||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:KRE9(YJL174W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:LSM4(YER112W)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MIM1(YOL026C)|FD-Score:3.84|P-value:6.19E-5|Clearance:0.43||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:SEC65(YML105C)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.02||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMX3(YPR182W)|FD-Score:-3.1|P-value:9.52E-4|Clearance:0||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:SPT16(YGL207W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.04||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TFC6(YDR362C)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YPI1(YFR003C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.01||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:BDP1(YNL039W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.03||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:DML1(YMR211W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.11||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG13(YML126C)|FD-Score:3.15|P-value:8.14E-4|Clearance:0.06||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:GUS1(YGL245W)|FD-Score:3.34|P-value:4.24E-4|Clearance:0.07||SGD DESC:Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:KRE9(YJL174W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:LSM4(YER112W)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MIM1(YOL026C)|FD-Score:3.84|P-value:6.19E-5|Clearance:0.43||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:SEC65(YML105C)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.02||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMX3(YPR182W)|FD-Score:-3.1|P-value:9.52E-4|Clearance:0||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:SPT16(YGL207W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.04||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TFC6(YDR362C)|FD-Score:-3.26|P-value:5.62E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YPI1(YFR003C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.01||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6166523
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:CBS2(YDR197W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CEX1(YOR112W)|FD-Score:-4.15|P-value:1.65E-5||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COX12(YLR038C)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CWC15(YDR163W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DYN2(YDR424C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:FIG1(YBR040W)|FD-Score:5.28|P-value:6.62E-8||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:HMG2(YLR450W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:IME1(YJR094C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:MAG1(YER142C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAP2(YBL091C)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MLC2(YPR188C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MTQ2(YDR140W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:PAI3(YMR174C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PFK1(YGR240C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POR2(YIL114C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:POX1(YGL205W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PRO2(YOR323C)|FD-Score:5.21|P-value:9.38E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS2(YER099C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PUF2(YPR042C)|FD-Score:4.88|P-value:5.38E-7||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RDH54(YBR073W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RPL43B(YJR094W-A)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS21A(YKR057W)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:SIA1(YOR137C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SKI2(YLR398C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SNA2(YDR525W-A)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOM1(YEL059C-A)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:STI1(YOR027W)|FD-Score:6.02|P-value:8.72E-10||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:STP4(YDL048C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIM21(YGR033C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TPP1(YMR156C)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP1(YDR007W)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VAB2(YEL005C)|FD-Score:7.1|P-value:6.32E-13||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YDR401W(YDR401W_d)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR015C(YGR015C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YHR180W(YHR180W_d)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL135W(YJL135W_d)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YLR271W(YLR271W_p)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YNR066C(YNR066C_p)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR277C(YOR277C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR333C(YOR333C_d)|FD-Score:-3.88|P-value:5.29E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth Gene:YOR385W(YOR385W_p)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPT53(YNL093W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:AAH1(YNL141W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:CBS2(YDR197W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CEX1(YOR112W)|FD-Score:-4.15|P-value:1.65E-5||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:COX12(YLR038C)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CWC15(YDR163W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DYN2(YDR424C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:FIG1(YBR040W)|FD-Score:5.28|P-value:6.62E-8||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:HMG2(YLR450W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:IME1(YJR094C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:MAG1(YER142C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MAP2(YBL091C)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MLC2(YPR188C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MTQ2(YDR140W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:PAI3(YMR174C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PFK1(YGR240C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIH1(YHR034C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POR2(YIL114C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:POX1(YGL205W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PRO2(YOR323C)|FD-Score:5.21|P-value:9.38E-8||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS2(YER099C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PUF2(YPR042C)|FD-Score:4.88|P-value:5.38E-7||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RDH54(YBR073W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RPL43B(YJR094W-A)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS21A(YKR057W)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:SIA1(YOR137C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SKI2(YLR398C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SNA2(YDR525W-A)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOM1(YEL059C-A)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs Gene:STI1(YOR027W)|FD-Score:6.02|P-value:8.72E-10||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:STP4(YDL048C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIM21(YGR033C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TPP1(YMR156C)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP1(YDR007W)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VAB2(YEL005C)|FD-Score:7.1|P-value:6.32E-13||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YDR401W(YDR401W_d)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR015C(YGR015C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YHR180W(YHR180W_d)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL135W(YJL135W_d)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YLR271W(YLR271W_p)|FD-Score:-3.82|P-value:6.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YNR066C(YNR066C_p)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR277C(YOR277C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR333C(YOR333C_d)|FD-Score:-3.88|P-value:5.29E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth Gene:YOR385W(YOR385W_p)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPT53(YNL093W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL026C3.846.19E-50.43MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YGL207W3.413.23E-40.04SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YNL039W3.373.75E-40.03BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
YGL245W3.344.24E-40.07GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YMR211W3.275.42E-40.11DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YFR003C3.167.85E-40.01YPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
YML126C3.158.14E-40.06ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YML105C3.099.98E-40.02SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YGL044C3.070.001070.15RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YFR002W2.920.001770.02NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YGL069C_d2.890.001900.02YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YPL012W2.880.002020.24RRP12Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats
YFL038C2.640.004150.04YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YGL065C2.600.004720.06ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YDL097C2.540.005620.01RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL005C7.106.32E-13VAB2Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter
YOR027W6.028.72E-10STI1Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
YBR040W5.286.62E-8FIG1Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating
YOR323C5.219.38E-8PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YKR057W5.111.60E-7RPS21AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
YNL141W5.002.81E-7AAH1Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome
YPR042C4.885.38E-7PUF2Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YBR073W4.741.06E-6RDH54DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p
YJR094W-A4.631.81E-6RPL43BRibosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YDR401W_d4.523.03E-6YDR401W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBL091C4.473.91E-6MAP2Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p
YLR398C4.261.00E-5SKI2Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YDL048C4.111.94E-5STP4Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress
YGR015C_p4.022.89E-5YGR015C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
YHR034C3.904.72E-5PIH1Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II

GO enrichment analysis for SGTC_877
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0881.60E-11SGTC_11453546-0597 10.2 μMChemDiv (Drug-like library)8753340.112676mitochondrial processes
0.0782.10E-9SGTC_9963970-0799 1.8 μMChemDiv (Drug-like library)66156480.0714286
0.0731.67E-8SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.0746269
0.0673.02E-7SGTC_1659st012954 42.1 μMTimTec (Natural product derivative library)242072620.0705882
0.0655.04E-7SGTC_30709117005 49.5 μMChembridge (Drug-like library)87790160.0704225
0.0631.50E-6SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.0731707amide catabolism
0.0603.74E-6SGTC_7710250-0039 27.3 μMChemDiv (Drug-like library)30051150.119403
0.0595.40E-6SGTC_6604031-1742 102.0 μMChemDiv (Drug-like library)67387060.12987redox potentiating
0.0596.59E-6SGTC_1802st050112 57.9 μMTimTec (Natural product derivative library)46625630.0506329
0.0552.22E-5SGTC_9531188-0045 9.7 μMChemDiv (Drug-like library)61444260.16949260S ribosome export
0.0543.06E-5SGTC_24855633444 17.8 μMMiscellaneous57242390.112676redox potentiating
0.0543.16E-5SGTC_10184259-0013 28.1 μMChemDiv (Drug-like library)68012620.0649351
0.0526.00E-5SGTC_31759106517 49.5 μMChembridge (Drug-like library)414680730.0985916iron homeostasis
0.0526.33E-5SGTC_20464110841 106.0 μMChembridge (Fragment library)441196300.0508475iron homeostasis
0.0518.34E-5SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.04

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9491165-0505146 μM0.4038466244267ChemDiv (Drug-like library)304.321082.15415
SGTC_840443-0269128.82 μM0.3508776150058ChemDiv (Drug-like library)266.294564.16703
SGTC_8610443-0273110 μM0.3272736149169ChemDiv (Drug-like library)268.267383.43914
SGTC_1813st0512468.6 μM0.3207555291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_1410443-027432.33 μM0.3157891627241ChemDiv (Drug-like library)268.267383.43914mitochondrial response to ROS
SGTC_5671080-054268.4 μM0.3050855950415ChemDiv (Drug-like library)296.277483.3115
SGTC_246457174157.67 μM0.2950825285744Miscellaneous318.37083.67115
SGTC_340469-068381.75 μM0.2931033690923ChemDiv (Drug-like library)337.307942.59217SWF1 & branched chain AA biosynthesis
SGTC_10024049-0206172 μM0.2881364662749ChemDiv (Drug-like library)310.3008432.90316
SGTC_24005203494178.7 μM0.286777674Miscellaneous399.398784.55216RSC complex & mRNA processing