0843-0131

N-[(Z)-(4-chlorophenyl)methylideneamino]-2-thiophen-2-ylacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_882
Screen concentration 351.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5425421
SMILES C1=CSC(=C1)CC(=O)NN=CC2=CC=C(C=C2)Cl
Standardized SMILES Clc1ccc(C=NNC(=O)Cc2cccs2)cc1
Molecular weight 278.7572
ALogP 3.34
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 32.84
% growth inhibition (Hom. pool) 3.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5425421
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.62|P-value:1.94E-6|Clearance:0.23||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BET4(YJL031C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.02||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CBF2(YGR140W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.08||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC11(YJR076C)|FD-Score:4.39|P-value:5.78E-6|Clearance:0.17||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CLF1(YLR117C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.08||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:DBF4(YDR052C)|FD-Score:-3.67|P-value:1.23E-4|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:FOL3(YMR113W)|FD-Score:3.15|P-value:8.20E-4|Clearance:0.05||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:MEC1(YBR136W)|FD-Score:6.6|P-value:2.12E-11|Clearance:1.32||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NOP1(YDL014W)|FD-Score:5.15|P-value:1.34E-7|Clearance:0.22||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSE4(YDL105W)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RLP24(YLR009W)|FD-Score:4.93|P-value:4.13E-7|Clearance:0.21||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB3(YIL021W)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.07||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:3.48|P-value:2.46E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C53 Gene:RPL33A(YPL143W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.13||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:SPN1(YPR133C)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.01||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRB7(YDR308C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:TIF34(YMR146C)|FD-Score:8.93|P-value:2.11E-19|Clearance:2.34||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TUB1(YML085C)|FD-Score:3.55|P-value:1.89E-4|Clearance:0.01||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP25(YIL091C)|FD-Score:5.28|P-value:6.49E-8|Clearance:0.13||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YGR114C(YGR114C_d)|FD-Score:4.69|P-value:1.37E-6|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR230W(YLR230W_d)|FD-Score:-5.14|P-value:1.38E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOL134C(YOL134C_d)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YOR146W(YOR146W_d)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ACC1(YNR016C)|FD-Score:4.62|P-value:1.94E-6|Clearance:0.23||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:BET4(YJL031C)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.02||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CBF2(YGR140W)|FD-Score:3.36|P-value:3.90E-4|Clearance:0.08||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC11(YJR076C)|FD-Score:4.39|P-value:5.78E-6|Clearance:0.17||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CLF1(YLR117C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.08||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:DBF4(YDR052C)|FD-Score:-3.67|P-value:1.23E-4|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:FOL3(YMR113W)|FD-Score:3.15|P-value:8.20E-4|Clearance:0.05||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:MEC1(YBR136W)|FD-Score:6.6|P-value:2.12E-11|Clearance:1.32||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NOP1(YDL014W)|FD-Score:5.15|P-value:1.34E-7|Clearance:0.22||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSE4(YDL105W)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RLP24(YLR009W)|FD-Score:4.93|P-value:4.13E-7|Clearance:0.21||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB3(YIL021W)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.07||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:3.48|P-value:2.46E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C53 Gene:RPL33A(YPL143W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.13||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:SPN1(YPR133C)|FD-Score:4.22|P-value:1.24E-5|Clearance:0.01||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRB7(YDR308C)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.06||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:TIF34(YMR146C)|FD-Score:8.93|P-value:2.11E-19|Clearance:2.34||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TUB1(YML085C)|FD-Score:3.55|P-value:1.89E-4|Clearance:0.01||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP25(YIL091C)|FD-Score:5.28|P-value:6.49E-8|Clearance:0.13||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YGR114C(YGR114C_d)|FD-Score:4.69|P-value:1.37E-6|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR230W(YLR230W_d)|FD-Score:-5.14|P-value:1.38E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YOL134C(YOL134C_d)|FD-Score:4.72|P-value:1.16E-6|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YOR146W(YOR146W_d)|FD-Score:3.72|P-value:9.89E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5425421
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACB1(YGR037C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE3(YGR204W)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADH4(YGL256W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ADH6(YMR318C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ALB1(YJL122W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ALR2(YFL050C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:ARA1(YBR149W)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:CPT1(YNL130C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:DBF20(YPR111W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DSS1(YMR287C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EFG1(YGR271C-A)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FRE6(YLL051C)|FD-Score:7.11|P-value:5.73E-13||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:FUR4(YBR021W)|FD-Score:-5.21|P-value:9.45E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GTT3(YEL017W)|FD-Score:6.36|P-value:1.03E-10||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HIS2(YFR025C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:JEM1(YJL073W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LOH1(YJL038C)|FD-Score:-4.67|P-value:1.48E-6||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MAL32(YBR299W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MKK1(YOR231W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:6.71|P-value:9.58E-12||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MRP20(YDR405W)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MUD1(YBR119W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MXR1(YER042W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAM2(YLR382C)|FD-Score:4.32|P-value:7.76E-6||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NGL1(YOL042W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OM14(YBR230C)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPI8(YKR035C_d)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:POR1(YNL055C)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RKM1(YPL208W)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPH1(YER169W)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL16B(YNL069C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL2B(YIL018W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL41B(YDL133C-A)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SOL4(YGR248W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SSA2(YLL024C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:TMA23(YMR269W)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TSR2(YLR435W)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:URA8(YJR103W)|FD-Score:4.28|P-value:9.47E-6||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VAC7(YNL054W)|FD-Score:4.97|P-value:3.32E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VHS2(YIL135C)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPH2(YKL119C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS21(YOR089C)|FD-Score:4.31|P-value:8.13E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YEH2(YLR020C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YFL052W(YFL052W_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHR140W(YHR140W_p)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Putative integral membrane protein of unknown function Gene:YKL018C-A(YKL018C-A_p)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR374C(YLR374C_d)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML090W(YML090W_d)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YML131W(YML131W_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YNL162W-A(YNL162W-A_p)|FD-Score:4.83|P-value:6.82E-7||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOL160W(YOL160W_d)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL102C(YPL102C_d)|FD-Score:8.04|P-value:4.59E-16||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPT53(YNL093W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:AAD6(YFL056C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACB1(YGR037C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE3(YGR204W)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADH4(YGL256W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ADH6(YMR318C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ALB1(YJL122W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ALR2(YFL050C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:ARA1(YBR149W)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:CPT1(YNL130C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:DBF20(YPR111W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DSS1(YMR287C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EFG1(YGR271C-A)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FRE6(YLL051C)|FD-Score:7.11|P-value:5.73E-13||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:FUR4(YBR021W)|FD-Score:-5.21|P-value:9.45E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GTT3(YEL017W)|FD-Score:6.36|P-value:1.03E-10||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HIS2(YFR025C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:JEM1(YJL073W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LOH1(YJL038C)|FD-Score:-4.67|P-value:1.48E-6||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MAL32(YBR299W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MKK1(YOR231W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:6.71|P-value:9.58E-12||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MRP20(YDR405W)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MUD1(YBR119W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MXR1(YER042W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAM2(YLR382C)|FD-Score:4.32|P-value:7.76E-6||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NGL1(YOL042W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OM14(YBR230C)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPI8(YKR035C_d)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:POR1(YNL055C)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RKM1(YPL208W)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPH1(YER169W)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL16B(YNL069C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL2B(YIL018W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL41B(YDL133C-A)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SOL4(YGR248W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SSA2(YLL024C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:TMA23(YMR269W)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TSR2(YLR435W)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:URA8(YJR103W)|FD-Score:4.28|P-value:9.47E-6||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VAC7(YNL054W)|FD-Score:4.97|P-value:3.32E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VHS2(YIL135C)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPH2(YKL119C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS21(YOR089C)|FD-Score:4.31|P-value:8.13E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YEH2(YLR020C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YFL052W(YFL052W_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity Gene:YHR140W(YHR140W_p)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Putative integral membrane protein of unknown function Gene:YKL018C-A(YKL018C-A_p)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR374C(YLR374C_d)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YML090W(YML090W_d)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YML131W(YML131W_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YNL162W-A(YNL162W-A_p)|FD-Score:4.83|P-value:6.82E-7||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOL160W(YOL160W_d)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL102C(YPL102C_d)|FD-Score:8.04|P-value:4.59E-16||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPT53(YNL093W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR146C8.932.11E-192.34TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YBR136W6.602.12E-111.32MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YIL091C5.286.49E-80.13UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YDL014W5.151.34E-70.22NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YLR009W4.934.13E-70.21RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YOL134C_d4.721.16E-60.03YOL134C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
YGR114C_d4.691.37E-60.07YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YNR016C4.621.94E-60.23ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YJR076C4.395.78E-60.17CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YPR133C4.221.24E-50.01SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YGR115C_d4.201.32E-50.48YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YOR146W_d3.729.89E-50.09YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YLR117C3.631.40E-40.08CLF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
YML085C3.551.89E-40.01TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YDR308C3.541.99E-40.06SRB7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL102C_d8.044.59E-16YPL102C_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4
YLL051C7.115.73E-13FRE6Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
YHR204W6.719.58E-12MNL1Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation
YEL017W6.361.03E-10GTT3Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery
YNL054W4.973.32E-7VAC7Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock
YML090W_d4.836.80E-7YML090W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source
YNL162W-A_p4.836.82E-7YNL162W-A_pPutative protein of unknown function; identified by homology
YHR140W_p4.572.44E-6YHR140W_pPutative integral membrane protein of unknown function
YLR435W4.552.71E-6TSR2Protein with a potential role in pre-rRNA processing
YDR405W4.473.99E-6MRP20Mitochondrial ribosomal protein of the large subunit
YLR382C4.327.76E-6NAM2Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
YOR089C4.318.13E-6VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YNL055C4.318.16E-6POR1Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication
YJR103W4.289.47E-6URA8Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
YOL042W4.221.20E-5NGL1Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_882
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0808.03E-10SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.107143translation
0.0699.43E-8SGTC_3021094-0063 18.9 μMChemDiv (Drug-like library)54402890.135135
0.0699.85E-8SGTC_7560508-1999 28.3 μMChemDiv (Drug-like library)67538530.136986RPP1 & pyrimidine depletion
0.0681.89E-7SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.146667translation
0.0672.28E-7SGTC_544k015-0020 52.4 μMChemDiv (Drug-like library)30083040.0952381
0.0649.86E-7SGTC_22176934799 96.1 μMChembridge (Fragment library)6756060.129032TSC3-RPN4
0.0631.29E-6SGTC_10503232-1156 69.3 μMChemDiv (Drug-like library)7005290.109375RSC complex & mRNA processing
0.0631.44E-6SGTC_15141189-1591 158.0 μMChemDiv (Drug-like library)7676980.0882353
0.0571.26E-5SGTC_3214534-3890 65.3 μMChemDiv (Drug-like library)6997460.162162RPP1 & pyrimidine depletion
0.0543.25E-5SGTC_6851278-0033 54.9 μMChemDiv (Drug-like library)35905720.051724160S ribosome export
0.0543.52E-5SGTC_11544088-0362 150.0 μMChemDiv (Drug-like library)66183040.0857143
0.0534.31E-5SGTC_10995-nonyloxytryptamine 2.9 μMNIH Clinical Collection235818250.0540541
0.0519.26E-5SGTC_1040074-0074 591.0 μMChemDiv (Drug-like library)39431240.134328
0.0519.65E-5SGTC_309k408-0051 1.5 μMChemDiv (Drug-like library)36404630.102273
0.0501.19E-4SGTC_631k072-0230 41.5 μMChemDiv (Drug-like library)57731470.26153860S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_24635473429200 μM0.5416675399405Miscellaneous273.717582.23113ERG2
SGTC_24605552655174.22 μM0.450985401989Miscellaneous259.6912.62513amide catabolism
SGTC_11841269-1718139 μM0.4339625403821ChemDiv (Drug-like library)260.311582.6214
SGTC_24675474540200 μM0.4259265413519Miscellaneous276.718222.92313
SGTC_7051124-0212310 μM0.4038469554485ChemDiv (Drug-like library)293.1484.01212
SGTC_9252064-127259.6 μM0.3770496815817ChemDiv (Drug-like library)333.726481.44825
SGTC_2995906776771.43 μM0.35087711673567Chembridge (Drug-like library)280.773123.18222
SGTC_12011889-337245.9 μM0.3448285392199ChemDiv (Drug-like library)282.337023.49113Golgi
SGTC_12001643-019318.4 μM0.3432845403974ChemDiv (Drug-like library)347.753063.56315
SGTC_13631636-006310.4 μM0.3392866072185ChemDiv (Drug-like library)344.815323.95914Golgi