0851-0618

N-[(E)-(4-iodophenyl)methylideneamino]-4-morpholin-4-yl-6-pyrrolidin-1-yl-1,3,5-triazin-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_884
Screen concentration 334.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 9642473
SMILES C1CCN(C1)C2=NC(=NC(=N2)NN=CC3=CC=C(C=C3)I)N4CCOCC4
Standardized SMILES Ic1ccc(C=NNc2nc(nc(n2)N3CCOCC3)N4CCCC4)cc1
Molecular weight 479.3181
ALogP 4.18
H-bond donor count 1
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.23
% growth inhibition (Hom. pool) 2.38


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9642473
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.09||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:CWC25(YNL245C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.03||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:ERG25(YGR060W)|FD-Score:-3.79|P-value:7.59E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:FMN1(YDR236C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.66||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLE1(YDL207W)|FD-Score:3.12|P-value:9.07E-4|Clearance:0.09||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:ILV3(YJR016C)|FD-Score:4.4|P-value:5.32E-6|Clearance:0.66||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:IMP3(YHR148W)|FD-Score:-3.43|P-value:2.96E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NUF2(YOL069W)|FD-Score:4.97|P-value:3.30E-7|Clearance:0.66||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:ORC4(YPR162C)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.14||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPB2(YOR151C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPC40(YPR110C)|FD-Score:-6|P-value:1.01E-9|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RRP36(YOR287C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.07||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:SGD1(YLR336C)|FD-Score:4.34|P-value:6.98E-6|Clearance:0.66||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SUA5(YGL169W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.03||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SWC4(YGR002C)|FD-Score:-3.22|P-value:6.51E-4|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TFB3(YDR460W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.2||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TYS1(YGR185C)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.01||SGD DESC:Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress Gene:URA6(YKL024C)|FD-Score:-3.8|P-value:7.20E-5|Clearance:0||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) Gene:UTP4(YDR324C)|FD-Score:-3.14|P-value:8.49E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YNL181W(YNL181W_p)|FD-Score:4.97|P-value:3.39E-7|Clearance:0.66||SGD DESC:Putative oxidoreductase; required for cell viability Gene:ADE13(YLR359W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.09||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:CWC25(YNL245C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.03||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:ERG25(YGR060W)|FD-Score:-3.79|P-value:7.59E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:FMN1(YDR236C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.66||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLE1(YDL207W)|FD-Score:3.12|P-value:9.07E-4|Clearance:0.09||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:ILV3(YJR016C)|FD-Score:4.4|P-value:5.32E-6|Clearance:0.66||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:IMP3(YHR148W)|FD-Score:-3.43|P-value:2.96E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NUF2(YOL069W)|FD-Score:4.97|P-value:3.30E-7|Clearance:0.66||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:ORC4(YPR162C)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.14||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPB2(YOR151C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPC40(YPR110C)|FD-Score:-6|P-value:1.01E-9|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RRP36(YOR287C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.07||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:SGD1(YLR336C)|FD-Score:4.34|P-value:6.98E-6|Clearance:0.66||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SUA5(YGL169W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.03||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SWC4(YGR002C)|FD-Score:-3.22|P-value:6.51E-4|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:TFB3(YDR460W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.2||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TYS1(YGR185C)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.01||SGD DESC:Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress Gene:URA6(YKL024C)|FD-Score:-3.8|P-value:7.20E-5|Clearance:0||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) Gene:UTP4(YDR324C)|FD-Score:-3.14|P-value:8.49E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YNL181W(YNL181W_p)|FD-Score:4.97|P-value:3.39E-7|Clearance:0.66||SGD DESC:Putative oxidoreductase; required for cell viability

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9642473
Download HOP data (tab-delimited text)  (excel)
Gene:ANT1(YPR128C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARC18(YLR370C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASC1(YMR116C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATC1(YDR184C)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG22(YCL038C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AZR1(YGR224W)|FD-Score:5.29|P-value:6.11E-8||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BDS1(YOL164W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BUD7(YOR299W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CPD1(YGR247W)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:DIA4(YHR011W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DOM34(YNL001W)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:ERP6(YGL002W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:FDC1(YDR539W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FEN1(YCR034W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FUM1(YPL262W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAL7(YBR018C)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GEP7(YGL057C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLE2(YER107C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPG1(YGL121C)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:ICL1(YER065C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:ILV1(YER086W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IRC15(YPL017C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LOC1(YFR001W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MCK1(YNL307C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MET13(YGL125W)|FD-Score:-3.41|P-value:3.31E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MRP49(YKL167C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSC1(YML128C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSS11(YMR164C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NFI1(YOR156C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NGL2(YMR285C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:OSM1(YJR051W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAA1(YDR071C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PEP4(YPL154C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PER1(YCR044C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PRD1(YCL057W)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PTR2(YKR093W)|FD-Score:4.91|P-value:4.55E-7||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RIM20(YOR275C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIT1(YMR283C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL14B(YHL001W)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRD1(YIL153W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RTK1(YDL025C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RVS161(YCR009C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SCD6(YPR129W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SHH4(YLR164W)|FD-Score:5.21|P-value:9.32E-8||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SNO1(YMR095C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPO22(YIL073C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SSN3(YPL042C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSO1(YPL232W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:TRM82(YDR165W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBP9(YER098W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YCR100C(YCR100C_p)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Putative protein of unknown function Gene:YIL029C(YIL029C_p)|FD-Score:4.81|P-value:7.39E-7||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL067C(YIL067C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL043W(YJL043W_p)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YLR031W(YLR031W_p)|FD-Score:-3.97|P-value:3.56E-5||SGD DESC:Putative protein of unknown function Gene:YOL035C(YOL035C_d)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YRF1-6(YNL339C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:ANT1(YPR128C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARC18(YLR370C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASC1(YMR116C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATC1(YDR184C)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG22(YCL038C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AZR1(YGR224W)|FD-Score:5.29|P-value:6.11E-8||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BDS1(YOL164W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BUD7(YOR299W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CPD1(YGR247W)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:DIA4(YHR011W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DOM34(YNL001W)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:ERP6(YGL002W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:FDC1(YDR539W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FEN1(YCR034W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FUM1(YPL262W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAL7(YBR018C)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GEP7(YGL057C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLE2(YER107C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GPG1(YGL121C)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:ICL1(YER065C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:ILV1(YER086W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IRC15(YPL017C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:LOC1(YFR001W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MCK1(YNL307C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MET13(YGL125W)|FD-Score:-3.41|P-value:3.31E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MRP49(YKL167C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSC1(YML128C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSS11(YMR164C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NFI1(YOR156C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NGL2(YMR285C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:OSM1(YJR051W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAA1(YDR071C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PEP4(YPL154C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PER1(YCR044C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PRD1(YCL057W)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PTR2(YKR093W)|FD-Score:4.91|P-value:4.55E-7||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RIM20(YOR275C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIT1(YMR283C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL14B(YHL001W)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRD1(YIL153W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RTK1(YDL025C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RVS161(YCR009C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SCD6(YPR129W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SHH4(YLR164W)|FD-Score:5.21|P-value:9.32E-8||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SNO1(YMR095C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPO22(YIL073C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SSN3(YPL042C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SSO1(YPL232W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:TRM82(YDR165W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UBP9(YER098W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YCR100C(YCR100C_p)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Putative protein of unknown function Gene:YIL029C(YIL029C_p)|FD-Score:4.81|P-value:7.39E-7||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL067C(YIL067C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL043W(YJL043W_p)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YLR031W(YLR031W_p)|FD-Score:-3.97|P-value:3.56E-5||SGD DESC:Putative protein of unknown function Gene:YOL035C(YOL035C_d)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YRF1-6(YNL339C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL069W4.973.30E-70.66NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YNL181W_p4.973.39E-70.66YNL181W_pPutative oxidoreductase; required for cell viability
YDR236C4.523.16E-60.66FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YJR016C4.405.32E-60.66ILV3Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
YLR336C4.346.98E-60.66SGD1Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
YLR359W3.681.16E-40.09ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YGL169W3.591.65E-40.03SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YDR460W3.561.86E-40.20TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YOR287C3.363.84E-40.07RRP36Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
YNL245C3.304.91E-40.03CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YGR185C3.265.51E-40.01TYS1Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
YPR162C3.265.63E-40.14ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YDL207W3.129.07E-40.09GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YPL128C3.030.001230.09TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YER126C2.930.001670.00NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR018C6.212.67E-10GAL7Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism
YGR224W5.296.11E-8AZR1Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole
YLR164W5.219.32E-8SHH4Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner
YBR293W4.934.01E-7VBA2Permease of basic amino acids in the vacuolar membrane
YKR093W4.914.55E-7PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YIL029C_p4.817.39E-7YIL029C_pPutative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W
YMR164C4.631.84E-6MSS11Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals
YLR370C4.582.32E-6ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YCR009C4.415.20E-6RVS161Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress
YGR247W4.376.30E-6CPD1Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress
YOR275C4.356.85E-6RIM20Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
YHR011W4.337.49E-6DIA4Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth
YCL038C4.121.90E-5ATG22Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation
YOL035C_d4.092.18E-5YOL035C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR034W4.082.26E-5FEN1Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_884
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0741.21E-8SGTC_1903st060211 78.3 μMTimTec (Natural product derivative library)6889600.0547945
0.0655.26E-7SGTC_1664st014217 9.0 μMTimTec (Natural product derivative library)39827820.044943860S ribosome export
0.0586.93E-6SGTC_23307979373 200.0 μMChembridge (Fragment library)18630070.069444460S ribosome export
0.0589.07E-6SGTC_13792144-0755 36.5 μMChemDiv (Drug-like library)53923270.133333
0.0571.33E-5SGTC_2130NSC 7 2.5 μMChembridge (Fragment library)214480.047619copper-dependent oxidative stress
0.0552.77E-5SGTC_23186029776 164.0 μMChembridge (Fragment library)8183250.0735294
0.0534.41E-5SGTC_1576erythromycin ethylsuccinate 23.2 μMTimTec (Pure natural product library)32560.023622
0.0525.84E-5SGTC_11760848-0131 83.3 μMChemDiv (Drug-like library)67690400.0864198Golgi
0.0525.92E-5SGTC_1705st027868 56.3 μMTimTec (Natural product derivative library)2929480.0769231
0.0518.20E-5SGTC_32359132602 49.5 μMChembridge (Drug-like library)49122400.16
0.0519.46E-5SGTC_1474466-0538 29.2 μMChemDiv (Drug-like library)67426870.0597015ergosterol biosynthesis
0.0501.11E-4SGTC_8860866-0150 153.0 μMChemDiv (Drug-like library)2714630.0746269
0.0491.63E-4SGTC_13833049-0032 20.7 μMChemDiv (Drug-like library)7128640.083333360S ribosome export
0.0472.98E-4SGTC_29469065502 71.4 μMChembridge (Drug-like library)164891010.0714286
0.0473.18E-4SGTC_22667929956 200.0 μMChembridge (Fragment library)29671530.0694444

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_11961588-0294184 μM0.547176144269ChemDiv (Drug-like library)355.4125834.57918
SGTC_12770873-003622 μM0.355965735ChemDiv (Drug-like library)411.500664.78239PDR1
SGTC_774k060-0086136 μM0.2656255911235ChemDiv (Drug-like library)280.324443.67815
SGTC_8650488-0499301 μM0.2586219634651ChemDiv (Drug-like library)203.200661.58426
SGTC_24435493264126.67 μM0.2580655340055Miscellaneous269.321642.39725
SGTC_1689st02026553.49 μM0.255758320TimTec (Natural product derivative library)336.38443.40405
SGTC_1824st05372368.6 μM0.245902675225TimTec (Natural product derivative library)291.347083.39704
SGTC_2427554505542.75 μM0.2459025372858Miscellaneous250.298463.71113
SGTC_623k072-010816.9 μM0.2419355382803ChemDiv (Drug-like library)254.31032.92315heme biosynthesis & mitochondrial translocase
SGTC_213453248361.46 μM0.236364699272Chembridge (Fragment library)258.272561.23804