0929-0073

(5Z)-5-(1H-indol-3-ylmethylidene)-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_891
Screen concentration 133.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5603632
SMILES C1=CC=C2C(=C1)C(=CN2)C=C3C(=O)NC(=O)S3
Standardized SMILES O=C1NC(=O)C(=Cc2c[nH]c3ccccc23)S1
Molecular weight 244.2691
ALogP 2.24
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.11
% growth inhibition (Hom. pool) 7.53


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5603632
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.06||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CWC25(YNL245C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.01||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:ERG26(YGL001C)|FD-Score:3.2|P-value:6.88E-4|Clearance:0.04||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:NOP9(YJL010C)|FD-Score:3.8|P-value:7.09E-5|Clearance:0.07||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:RPL3(YOR063W)|FD-Score:3.73|P-value:9.46E-5|Clearance:0.47||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RVB2(YPL235W)|FD-Score:5.54|P-value:1.55E-8|Clearance:1.73||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SPC110(YDR356W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.01||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TPI1(YDR050C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.02||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:YCG1(YDR325W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL152W(YDL152W_d)|FD-Score:-3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:AFG2(YLR397C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.06||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CWC25(YNL245C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.01||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:ERG26(YGL001C)|FD-Score:3.2|P-value:6.88E-4|Clearance:0.04||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:NOP9(YJL010C)|FD-Score:3.8|P-value:7.09E-5|Clearance:0.07||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:RPL3(YOR063W)|FD-Score:3.73|P-value:9.46E-5|Clearance:0.47||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RVB2(YPL235W)|FD-Score:5.54|P-value:1.55E-8|Clearance:1.73||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SPC110(YDR356W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.01||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TPI1(YDR050C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.02||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:YCG1(YDR325W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL152W(YDL152W_d)|FD-Score:-3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5603632
Download HOP data (tab-delimited text)  (excel)
Gene:ARG2(YJL071W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARO1(YDR127W)|FD-Score:4.88|P-value:5.21E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO9(YHR137W)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG19(YOL082W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:BCH1(YMR237W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BIO3(YNR058W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CCC1(YLR220W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CDC10(YCR002C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:COG6(YNL041C)|FD-Score:-4.56|P-value:2.57E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX13(YGL191W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:COX7(YMR256C)|FD-Score:4.54|P-value:2.80E-6||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRG1(YHR209W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:ECM33(YBR078W)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FTR1(YER145C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FUS1(YCL027W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GAS1(YMR307W)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN20(YFR009W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.85|P-value:6.25E-7||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.75|P-value:4.56E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPA2(YER020W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:IES5(YER092W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:MDH1(YKL085W)|FD-Score:-3.19|P-value:7.05E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MRPS8(YMR158W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NKP1(YDR383C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:PAU11(YGL261C_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PGD1(YGL025C)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO23(YNL097C)|FD-Score:4.47|P-value:3.93E-6||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PRY3(YJL078C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:PXL1(YKR090W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RDL2(YOR286W)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:REC102(YLR329W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RPN4(YDL020C)|FD-Score:-7.42|P-value:5.75E-14||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SCW10(YMR305C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SFM1(YOR021C)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SHC1(YER096W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIN3(YOL004W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SNT309(YPR101W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SRO77(YBL106C)|FD-Score:4.83|P-value:6.73E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:TIP1(YBR067C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TPS3(YMR261C)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP13(YBL067C)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:YBR139W(YBR139W)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBR141C(YBR141C_p)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL049C(YCL049C_p)|FD-Score:-3.74|P-value:9.07E-5||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.74|P-value:7.91E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:-4.47|P-value:3.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YEF1(YEL041W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFL012W(YFL012W_p)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFR006W(YFR006W_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR050C(YGR050C_p)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR053C(YGR053C_p)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Putative protein of unknown function Gene:YGR093W(YGR093W_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YHL008C(YHL008C_p)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIL032C(YIL032C_d)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL043W(YJL043W_p)|FD-Score:4.4|P-value:5.29E-6||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YMR206W(YMR206W_p)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YMR253C(YMR253C_p)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YOL035C(YOL035C_d)|FD-Score:5.1|P-value:1.67E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR309C(YOR309C_d)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:ARG2(YJL071W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARO1(YDR127W)|FD-Score:4.88|P-value:5.21E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO9(YHR137W)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG19(YOL082W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:BCH1(YMR237W)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BIO3(YNR058W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CCC1(YLR220W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CDC10(YCR002C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:COG6(YNL041C)|FD-Score:-4.56|P-value:2.57E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX13(YGL191W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:COX7(YMR256C)|FD-Score:4.54|P-value:2.80E-6||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRG1(YHR209W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:ECM33(YBR078W)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FTR1(YER145C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FUS1(YCL027W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GAS1(YMR307W)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN20(YFR009W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.85|P-value:6.25E-7||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.75|P-value:4.56E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPA2(YER020W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:IES5(YER092W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:MDH1(YKL085W)|FD-Score:-3.19|P-value:7.05E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MRPS8(YMR158W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NKP1(YDR383C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:PAU11(YGL261C_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PGD1(YGL025C)|FD-Score:4.33|P-value:7.59E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO23(YNL097C)|FD-Score:4.47|P-value:3.93E-6||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PRY3(YJL078C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:PXL1(YKR090W)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RDL2(YOR286W)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss Gene:REC102(YLR329W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RPN4(YDL020C)|FD-Score:-7.42|P-value:5.75E-14||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SCW10(YMR305C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SFM1(YOR021C)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SHC1(YER096W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIN3(YOL004W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SNT309(YPR101W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SRO77(YBL106C)|FD-Score:4.83|P-value:6.73E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:TIP1(YBR067C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TPS3(YMR261C)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP13(YBL067C)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:YBR139W(YBR139W)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBR141C(YBR141C_p)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL049C(YCL049C_p)|FD-Score:-3.74|P-value:9.07E-5||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.74|P-value:7.91E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:-4.47|P-value:3.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YEF1(YEL041W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFL012W(YFL012W_p)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFR006W(YFR006W_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR050C(YGR050C_p)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR053C(YGR053C_p)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Putative protein of unknown function Gene:YGR093W(YGR093W_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YHL008C(YHL008C_p)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIL032C(YIL032C_d)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL043W(YJL043W_p)|FD-Score:4.4|P-value:5.29E-6||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YMR206W(YMR206W_p)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YMR253C(YMR253C_p)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YOL035C(YOL035C_d)|FD-Score:5.1|P-value:1.67E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR309C(YOR309C_d)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL235W5.541.55E-81.73RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YJL010C3.807.09E-50.07NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YOR063W3.739.46E-50.47RPL3Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus
YLR397C3.265.58E-40.06AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YGL001C3.206.88E-40.04ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YDR325W3.167.85E-40.04YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YDR356W3.129.13E-40.01SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YNL245C3.119.30E-40.01CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YDR050C3.109.69E-40.02TPI1Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease
YGL061C3.080.001020.04DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YIL106W3.040.001180.04MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YDL097C3.000.001365.90E-4RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YDR160W3.000.001370.07SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YEL026W2.920.001730.01SNU13RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein
YNR035C2.910.001810.16ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d6.747.91E-12YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YEL009C5.754.56E-9GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YMR261C5.257.55E-8TPS3Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
YOL035C_d5.101.67E-7YOL035C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR307W5.091.77E-7GAS1Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery
YDR127W4.885.21E-7ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YKR026C4.856.25E-7GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YBL106C4.836.73E-7SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YKL211C4.621.90E-6TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YBR196C-B_p4.582.37E-6YBR196C-B_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YMR256C4.542.80E-6COX7Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YOR309C_d4.513.24E-6YOR309C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
YNL097C4.473.93E-6PHO23Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3
YIL092W_p4.464.09E-6YIL092W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
YHR137W4.415.19E-6ARO9Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism

GO enrichment analysis for SGTC_891
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2882.26E-113SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.057971
0.2541.82E-87SGTC_8900929-0006 47.2 μMChemDiv (Drug-like library)13781690.321429
0.2541.90E-87SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.0657895
0.2531.58E-86SGTC_18825615643 20.0 μMMiscellaneous22530750.133333TRP & mitochondrial translation
0.2471.67E-82SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0337079
0.2446.95E-81SGTC_5423381-0244 89.3 μMChemDiv (Drug-like library)11128430.0833333
0.2415.66E-79SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.057971
0.2404.24E-78SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.0481928
0.2367.90E-76SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0493827
0.2361.91E-75SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.169231
0.2358.69E-75SGTC_24785763493 32.3 μMMiscellaneous13655760.220339
0.2342.17E-74SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0694444
0.2331.23E-73SGTC_24865283202 22.4 μMMiscellaneous13777510.413043
0.2251.47E-68SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.38
0.2247.20E-68SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.372549

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_440929-005561.21 μM0.5476191379590ChemDiv (Drug-like library)239.678142.60613
SGTC_2102537781672.76 μM0.4444441379702Chembridge (Fragment library)195.19521.33714
SGTC_12860929-0070131 μM0.441420408ChemDiv (Drug-like library)251.258461.68325
SGTC_1994st07294740.9 μM0.4385965286565TimTec (Natural product derivative library)397.44914.74823
SGTC_8920929-007629 μM0.4318185751038ChemDiv (Drug-like library)219.259662.42813TRP & mitochondrial translation
SGTC_12840929-004316 μM0.4318181239883ChemDiv (Drug-like library)223.2235432.14714
SGTC_205252651827.14 μM0.4318181239884Chembridge (Fragment library)223.2235432.14714
SGTC_380929-005158.27 μM0.4255321379719ChemDiv (Drug-like library)223.2235432.14714
SGTC_245553777129.75 μM0.4255322243908Miscellaneous219.259662.42813
SGTC_210849-011322.99 μM0.4130431560156ChemDiv (Drug-like library)188.182722.10312